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Citations for Thermo Fisher :
5801 - 5850 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The DNA fragment was labeled with 5-(3-aminoallyl)dUTP by nick translation according to the manufacturer’s protocol (Ares DNA labeling kit, Molecular Probes, Eugene, OR, USA), and the incorporated dUTPs were labeled with amino-reactive Alexa Fluor 647 dye by using the same Ares DNA labeling kit ...
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bioRxiv - Neuroscience 2022Quote: ... Dishes were washed with PBS 5 times for 3 min each before applying donkey anti-mouse Alexa Fluor 568 secondary antibodies (A10037, Invitrogen; 1:2000 diluted) and Alexa Fluor 647 phalloidin (A22287 ...
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bioRxiv - Neuroscience 2023Quote: ... and gravitational sedimentation by washing 3 times in wash media composed of DMEM/F12 supplemented with 5% fetal bovine serum (Thermo Fisher: 10082– 147) and 1000 U/ml penicillin/streptomycin ...
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bioRxiv - Genetics 2024Quote: ... followed by the addition of 300 ng of predesigned sgRNA targeting exon 3 region of the XPC gene with a crRNA sequence (5’AGGCACACCATCTGAAGAGA3’) (Thermofisher Scientific, Massachusetts, USA). The sgRNA and Cas9 mixture was incubated for 10 minutes at room temperature to assemble and form the ribonucleoprotein complex ...
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bioRxiv - Immunology 2024Quote: ... Primers were designed with 3’ BamHI and 5’ KpnI sites flanking each sequence and the fragment amplified using Phusion™ High-Fidelity DNA Polymerase (Thermo Fisher Scientific). Purified PCR product was digested with BamHI-HF and KpnI-HF (NEB ...
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bioRxiv - Microbiology 2024Quote: ... The DNA fragment was labeled with 5-(3-aminoallyl)dUTP by nick translation according to the manufacturer’s protocol (Ares DNA labeling kit, Molecular Probes, Eugene, OR, USA), and the incorporated dUTPs were labeled with amino-reactive Alexa Fluor 647 dye by using the same Ares DNA labeling kit.
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bioRxiv - Cell Biology 2024Quote: ... 2.0µM Template Switching Oligo (TSO) (5′-Biotin-AGAGACAGATTGCGCAATGNNNNNNNNrGrGrG-3′; IDT) and 2U µl−1 of Maxima H Minus reverse transcriptase (Thermo Fisher Scientific, #EP0752). Plates were quickly centrifuged before incubation at 42°C for 90min ...
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bioRxiv - Cell Biology 2024Quote: ... and membranes were washed 3×5 minutes in 1X TBS-T before being developed using Pierce SuperSignal West Pico PLUS (Thermo Scientific, cat: 34580) (β-catenin ...
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bioRxiv - Plant Biology 2022Quote: ... the specific entry clones described above (TRB1-3, 5 in pDONR207 and TRB4 in pENTR223) were used in LR Clonase™ II (Thermo Fisher Scientific) reactions to create the pGWB6 (N-terminal GFP fusion under the 35S promoter ...
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bioRxiv - Microbiology 2023Quote: ... and 0.5 µl of both forward and reverse primers (5 µM) were run on the QuantStudio™ 3 Real-Time PCR System (Thermo Fisher Scientific). The primers used in this study were ...
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bioRxiv - Genetics 2023Quote: ... RNA was extracted in pools of 10 from 3-5 days old females randomly selected and unexposed to any insecticides using Arcturus PicoPure RNA isolation kit (Applied Biosystems, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Both real-time PCR reactions were performed using Universal MasterMix and TaqMan MGB probes with 5’FAM and a 3’ nonfluorescent quencher (NFQ, Thermo Fisher, CA. USA). The obtained cycle threshold values were converted to an arbitrary amount by using standard curves from a serial dilution of a pooled sample made from all samples ...
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bioRxiv - Cell Biology 2022Quote: ... for 3 days at a density of 5 × 104 cells per well in a 96-well tissue culture plate (Thermo Fisher, Nunclon delta). The media was then replaced with fresh PMA-free media and the cells were left to rest for 24 h before the infection protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... rinsed with 0.1 M PBS (5-10 min x 3) at room temperature and then counterstained with DAPI (200 ng/ml in PBS; D1306, Life Technologies, Carlsbad, CA) at room temperature for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... total RNA was extracted from the SGs from SGs of 3-5-day-old fifth-instar larvae using Trizol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... to visualize CaSR-containing cells and for 5 minutes with 1:1000 TO-PRO-3 iodide (#T3605, Thermo Fisher Scientific, Waltham, MA, USA) to visualize permeable cells ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were then rinsed 3 times for 5 minutes in PBS and mounted on ColorFrost Plus slides (Fisher Scientific, Ottawa, Ontario, Canada) using Fluoromount-G (Southern Biotech ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was isolated from WT and Top1MT KO mice liver tissues of the same size (3 × 5 mm) using TRIzolTM (Thermo Fisher Scientific, #15596026). Quality check (RIN ≥ 8 ...
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bioRxiv - Immunology 2024Quote: ... The membranes were washed 3 times with TBS for 5 minutes each and then incubated with a secondary antibody (Thermo Fisher Scientific, US). An enhanced chemiluminescence (ECL ...
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bioRxiv - Cancer Biology 2024Quote: ... washed three times with TBS-T with moderate agitation 3 x 5 min and subsequently analyzed using enhanced chemi-luminescent substrate (ECL) (Thermo Fisher Scientific, 34578). Chemiluminescence was visualized using an iBright Imaging System (Invitrogen) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Nvsix3/6:venus was generated by subcloning the Nvsix3/6 coding sequence into pENTR/D TOPO (ThermoFisher Scientific) using published primers previously used to PCR amplify Nvsix3/6 and synthesize Nvsix3/6 mRNA 47 ...
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bioRxiv - Microbiology 2021Quote: ... thaliana seedlings cultured in 6 ml liquid MSgg of each well of a 6-well microplate (Thermo Scientific). 8 seedlings were put in each well ...
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bioRxiv - Microbiology 2020Quote: ... Cells in antibiotic free media (8×105/6-well) were transfected 6 hours post infection with 2μg plasmid and 6μl Lipofectamine 2000 (Invitrogen) per well diluted in Opti-MEM (Invitrogen).
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bioRxiv - Synthetic Biology 2024Quote: ... Jurkat cells and NALM-6 expressing firefly luciferase- GFP (NALM-6) were cultured in RPMI 1640 (Thermo Fisher) supplemented with 10% FBS and 100 units/mL penicillin and streptomycin ...
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bioRxiv - Genomics 2024Quote: ... C57BL /6 wildtype keratinocytes were seeded in a 6-well plate (Thermo Scientific Nunclon TM Delta Surface; 140675) at 1.5-3 × 105 cells per well ...
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bioRxiv - Cell Biology 2020Quote: ... and TagRFP-T-VAPB(1-218)-eMagB or TagRFP-T-eMagB-PHOSBP (prey) and iRFP-P4C at a 3:2:1 ratio in OptiMEM-I (Thermo Fisher Scientific) (1:4 DNA ...
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bioRxiv - Cell Biology 2020Quote: Total cellular mRNA was isolated from cells grown in 2-D and 3-D culture conditions using the GeneJET RNA purification kit (Thermo Fisher Scientific). For analysis of germ layer markers ...
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bioRxiv - Cell Biology 2020Quote: An aliquot of each sample equivalent to 3 μg was loaded onto a trap column (Acclaim PepMap 100 pre-column, 75 μm × 2 cm, C18, 3 μm, 100 Å, Thermo Scientific) connected to an analytical column (Acclaim PepMap RSLC column C18 2 μm ...
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bioRxiv - Biochemistry 2022Quote: ... The trap column used for the nano HPLC was a 2 cm × 75 μm capillary column packed with 3 μm C18-silica particles (Thermo Fisher Scientific) and the separation column was a 12.5 cm × 75 μm capillary column packed with 3 μm C18-silica particles (Nikkyo Technos Co. ...
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bioRxiv - Molecular Biology 2021Quote: ... Peptide mixtures were loaded on a C18 Acclaim PepMap100 trap-column (75 μm ID x 2 cm, 3 μm, 100Å, Thermo Fisher Scientific) for 3.5 minutes at 5 μL/min with 2% Acetonitrile MS grade (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... were concentrated and desalted on a trapping pre-column (Acclaim PepMap C18, 75 µm × 2 cm nanoViper, 3 µm, 100 Å, Thermo Scientific), using 0.2% formic acid at a flow rate of 5 µl/min ...
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bioRxiv - Neuroscience 2020Quote: ... and then 12 µg from each was injected into a 75 μm × 2 cm nanoViper C18 Acclaim PepMap100 precolumn (3 μm particle size, 100 Å pore size; P/N 164705; Thermo Scientific). Peptides were separated at a flow rate of 250 nl/min and eluted from the column over a 5 hours gradient starting with 0.1% FA in ACN (5-30% ACN from 0 to 280min followed by 10min ramping up to 80% ACN ...
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bioRxiv - Biochemistry 2020Quote: ... Peptide sample (2 μL) was injected onto an Acclaim PepMap 1000 trap column (75 μm × 2 cm, nanoViper C18 3 μm, 100Å, Thermo Fisher Scientific) which was heated to 40 °C in a chamber which was connected to a C18 reverse-phase Aurora UHPLC Emitter Column with nano Zero & Captive Spray Insert (75 μm × 25 cm ...
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bioRxiv - Biochemistry 2020Quote: ... N-((6-(2,4-DNP)Amino)Hexanoyl)-1-(BODIPY™ FL C5)-2-Hexyl-Sn-Glycero-3-Phosphoethanolamine (PED-A1) and BODIPY™ FL C5 were purchased from Thermofisher scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed with PBS (3 x 10 min) and incubated 2 hours with the appropriate secondary antibodies (Invitrogen, CA, USA) at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... washed 3 times with blocking buffer and incubated for 2 h with Alexa 647 anti-rabbit (1/250, A21247, Thermo Fisher Scientific). After washing the cells 3 times with PBS ...
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bioRxiv - Biochemistry 2021Quote: ... approximately 1 pmol injected onto a Acclaim PepMap 100 guard column (75 μm×2 cm C18, 3 μm particles, 100 Å; Thermo Scientific) and separated on a 50 cm PepMap RSLC C18 (75 μm×50 cm ...
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bioRxiv - Neuroscience 2020Quote: Cells were electroporated with 2 μg of donor construct and 3 μg of px330-Cas9 plasmid using a Neon Transfection System (Thermo Fisher Scientific) and the following settings ...
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bioRxiv - Biophysics 2020Quote: ... We grew HL-60 cells to approximately 1 × 106 cells/ml and passaged the cells every 2-3 days in Roswell Park Memorial Institute medium (RPMI-1640, Thermo Fisher Scientific) supplemented with L-glutamine and 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... BV-2 immortalized microglial cells (Blasi et al. 1990) (passage 3 to 8) cultured in macrophage serum-free medium (ThermoFisher 12065-074) were used ...
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bioRxiv - Biochemistry 2022Quote: The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Orbitrap Exploris 480 for replicate 2, and Q-Exactive HF for replicate 3, Thermo Fisher Scientific). Peptides were sampled on a precolumn (300 μm × 5 mm PepMap C18 ...
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bioRxiv - Immunology 2022Quote: ... Peptide lysates were injected on a trapping column (Acclaim PepMap 100 C18, 3 μm, nanoViper, 75 μm x 2 cm, Thermo Fisher Scientific) with 5 μl/min ...
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bioRxiv - Biochemistry 2021Quote: ... spectra were recorded on a Thermo-Finnigan Surveyor equipped with a Satisfaction RP18AB C18 3 μm (15×2 mm) (Cluseau) and coupled to an ESI LCQ Advantage (Thermo Fisher Scientific). The running mixture was subjected to filtering on a Microcon filter unit of 10 kDa (15 min ...
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bioRxiv - Microbiology 2021Quote: ... using an Acclaim PepMap100 pre-column (C18, 3 μm-100 Å) and a PepMap RSLC (C18, 2 μm, 50 μm-15 cm) both from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... incubating with primary antibodies (rabbit anti-β-actin, 1:1000, Thermo Fisher Scientific, PA1-183; mouse anti-claudin-2, 3:500, Invitrogen, 325600 ...
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bioRxiv - Cancer Biology 2020Quote: ... LC separation was executed with a trap column (C18, 75 μm*2 cm, 3 μm) (cat No. 164946, Thermo Fisher Scientific, Germany) and an analytical C18 column (C18 ...
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bioRxiv - Microbiology 2020Quote: ... were performed on an Ultimate 3000 RSLC nano instrument coupled to either a QExactive Plus (M#1, M#3) or an HF mass spectrometer (M#2; Thermo Fisher Scientific) as described previously.24 Tryptic peptides were trapped for 4 min on an Acclaim Pep Map 100 column (2 cm × 75 μm ...
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bioRxiv - Immunology 2020Quote: ... D-PBS (D-PBS without Ca++ and Mg++) supplemented with 2% FBS and 3 mM cell culture grade EDTA (Life Technologies; Thermofisher) prior to monocyte isolation ...
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bioRxiv - Genomics 2021Quote: Human brain tissues were manually dissected into small 2-3 mm3 pieces and immersed in homogenization buffer (HBSS (Life Technologies, 14175-095), 1% bovine serum albumin (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... Peptides were loaded onto a trap column (Dionex-C18 trap column 75 μm × 2 cm, 3 μm, particle size, 100 Å pore size; ThermoFisher Scientific) at 5 μl/min before switching the pre-column in line with the analytical column (Dionex-C18 analytical column 75 μm × 50 cm ...