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Citations for Thermo Fisher :
5751 - 5800 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: 2×106 THP-1 cells were centrifuged for 5 min at 1200 rpm and washed in 1X PBS (Gibco). They were then transfected by nucleofection with 3 μg of plasmid DNA in 100 μl of Cell Line Nucleofector Solution V (Lonza ...
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bioRxiv - Physiology 2024Quote: FAP proliferation was detected using 5-ethynyl-2’-deoxyuridine (EdU)/click-it assay (cat. no.: C10337 and C10340, Invitrogen). Experiments were carried out in ECM coated 96-well half-area plates ...
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bioRxiv - Pathology 2024Quote: ... Cells were incubated with 5 µM Fura-2-AM in 1 mL RPMI B27 + Insulin (Thermo Fisher Scientific 17504044) at 37°C for 20 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Cultures were maintained on blood agar base #2 (Oxoid) supplemented with 5 % (v/v) defibrinated horse blood (Thermo Scientific) and incubated under microaerobic conditions (5 % O2 ...
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bioRxiv - Immunology 2024Quote: ... murine BMDCs at 2 × 106 cells/mL were stained with 5 μM carboxy-H2DCFDA (Invitrogen™, Carlsbad, CA, USA) and pre-incubated with prepared fatty acids (1 mM ...
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bioRxiv - Neuroscience 2024Quote: ... Time-lapse videos were recorded at 25 Hz for 5□min at 37□°C and 5% CO2 in culture media (Neurobasal media, 2% B27, 0.5□mM Glutamate, and 1% Penicillin/Streptomycin (Gibco)) ...
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bioRxiv - Molecular Biology 2024Quote: ... using a pre-column for sample loading (Acclaim PepMap C18, 2 cm × 0.1 mm, 5 μm, Thermo-Fisher Scientific), and a C18 analytical column (Acclaim PepMap C18 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were transfected with 5-20 nM siRNA oligos (Table 2) using Lipofecatmine RNAiMAX transfecting agent (Invitrogen; #13778-075) according to the manufacturer’s instructions for 72 hours ...
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bioRxiv - Bioengineering 2024Quote: ... peptides were first trapped on a trap column (PepMap 100 µm × 2 cm, 5 µm particles, Thermo Fisher Scientific) using a flow rate of 5 µL min-1 and 0.1% formic acid ...
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bioRxiv - Cell Biology 2024Quote: ... Young (feeding) L3 larvae were collected and fed overnight with standard flyfood containing EdU (5-ethynyl-2’-deoxyuridine) (Invitrogen) and Bromophenol Blue sodium salt (Sigma) ...
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bioRxiv - Developmental Biology 2024Quote: ... then each blastocyst was added to 5 µl of 2 × Reaction Mix (Invitrogen, CellsDirect One-Step qRT-PCR Kit) and snap-frozen on dry ice and stored at -80°C until processing.
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bioRxiv - Developmental Biology 2024Quote: The 5-ethynyl-2’-deoxyuridine (EdU) assay was performed using a click it-488 (C10337 and Click-it, Invitrogen) by soaking the dissected CNS for 1 hr ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were secured with a glass anchor in the bottom of a 35 mm petri dish and incubated in carbogen (5% CO2 and 95% oxygen) bubbled 3,3’-Diaminobenzidine (DAB; ACROS Organics; code: 328005000, CAS: 7411-49-6) solution (1 mg ml-1 in PBS ...
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bioRxiv - Bioengineering 2022Quote: ... and 0.02 mM 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) (Invitrogen). The reaction was allowed to proceed on a rotary incubator at room temperature for at least 4 hours ...
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bioRxiv - Biophysics 2022Quote: ... 19 mg EDC (1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide) (Thermo Fisher), 11 mg sulfo-NHS (N-Hydroxysulfosuccinimide ...
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bioRxiv - Immunology 2022Quote: ... 1.9g of ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) (ThermoFisher #22980) and 1.2g of N-hydroxysuccinimide (NHS ...
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bioRxiv - Bioengineering 2023Quote: ... and 0.02 mM 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) (Invitrogen). The reaction was performed on a rotary incubator at room temperature for at least 4 hours ...
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bioRxiv - Bioengineering 2024Quote: ... and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, Thermo Scientific, 22980) were added to the alginate solution in a molar ratio of 1 alginate:30 NHS:25 EDC ...
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bioRxiv - Biophysics 2024Quote: ... and 3-pyridinemethanol (RI = 1.545, 3-PM, A10381, Thermo Fisher Scientific) were used to mix with the water-based SMLM buffer ...
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bioRxiv - Cell Biology 2024Quote: ... human custom-made 3’UTR HEC1 siRNA (oligo #3, Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... two SlP4H3 OEX lines (#1, #2) and three SlP4H3 RNAi lines (#1, #6 and #7) by using the TRI reagent (Thermo Fisher Scientific, Waltham, MA, USA). Reverse transcription of approximately 1 μg of total RNA was performed with Superscript II® Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: Lines with defective stomatal responses to at least one stimulus were subjected to sequencing of “usual suspects” either by NGS-based sequencing of PCR amplicons obtained with the use of gene-specific primers (Supplementary Data Set 2) and Phusion DNA polymerase (ThermoFisher Scientific; ROUND 1 to 6), or whole-genome sequencing (ROUND 7 and 8) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Several donor colonies and one recipient colony were independently inoculated in 2 mL of LB in 15-mL tubes and cultured for 6 hours (37°C and 250 rpm, Thermo Scientific™ MaxQ™ 8000). Cultures were centrifuged (15 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... Enzyme assays were conducted in 0.5 mL containing 50 mM 2-(N-morpholino)ethanesulfonic acid (pH 6) and various concentrations of soluble starch (Acros Organics, No. AC424495000, Morris Plains, NJ). The assays also included 100 mM KCl ...
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFUSE-vector (Invitrogen, Thermo Fischer Scientific, MA, USA). The human ACE2 was expressed by essentially the same protocol used for the COVID-19 RBD ...
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bioRxiv - Cancer Biology 2022Quote: ... To quantitate cell migration 6 fields of view per membrane were photographed with EVOS™ FL Auto 2 Imaging System(Thermo Scientific™ Invitrogen™), followed by quantification by ImageJ v1.53q (National Institutes of Health ...
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bioRxiv - Neuroscience 2024Quote: ... then separated on a reverse-phase nano-spray column (2 μm, 75 mm i.d. × 50 cm, 100 Å, Thermo Fisher Scientific Cat# 16-494-6) using gradient elution ...
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bioRxiv - Microbiology 2020Quote: ... and resuspended in 0.1 ml of PBS supplemented with the lipophilic styryl membrane dye N-(3-triethylammoniumprpyl)-4-(p-diethylaminophenyl-hexatrienyl) pyridinium dibromide (FM4-64, Molecular Probes, Invitrogen; 10 μg.ml-1) (Pogliano et al. ...
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bioRxiv - Immunology 2021Quote: 293T cells grown in 96-well plates (3-4×104 cells / well) were co-transfected by Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA USA) with reporter plasmids ...
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bioRxiv - Neuroscience 2021Quote: ... Neurons were transfected at DIV 3-4 with mRFP-Rab7 (0.8 μg DNA per well) using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA) followed by fixation (4% PFA ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was centrifuged at 1000 g for 3 min at 4 °C to remove debris and then the supernatant was incubated with anti-HA magnetic beads (Thermo Fisher Scientific, cat. # 88837) for 6 minutes at 20 °C ...
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bioRxiv - Biochemistry 2023Quote: ... blotted from both sides for 3 seconds at 4 ℃ under 99% humidity and plunge frozen in liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific, Waltham, MA). Images were recorded using a Titan Krios G4 microscope at the University of Tokyo (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: EAT (occluded and non-occluded tissue pooled) from exercise-trained (n=4) and sedentary (n=3) female pigs had total RNA extracted with TRIzol® (Thermofisher Scientific, Waltham, MA). Total RNA was quantified (Nanodrop 3300 ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were then incubated for 3 h at 4 °C with 50 mL of Pierce Protein-G beads bound with mouse anti-Ty antibody (Invitrogen Ty1 Tag Monoclonal BB2). Beads were washed 6 times with wash buffer (200 mM Tris pH 9 ...
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bioRxiv - Neuroscience 2024Quote: ... and diluted secondary antibodies were applied at 4 °C for approximately 3 hours using Alexa Fluor 488 goat anti-rabbit (1:1000; Invitrogen, Waltham, MA; Catalog #A11008); Alexa Fluor Plus 555 goat anti-rat (1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... target cancer cells plated in 3 or 4 replicates in 96-well plates were labeled with CellTracker Green CMFDA Dye (Thermo Fisher Scientific, Cat# C7025), and incubated with NK cells at a 1:4 carcinoma to NK cell ratio ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were then washed 3 times for 5 minutes each in PBS before incubation with secondary goat anti-rabbit 647 (ThermoFisher Scientific, cat #A21244) and goat anti-rat 568 (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... The resulting pellet was allowed to air-dry for 5 min and finally resuspended in molecular biology grade H2O for quantitation using a Qubit 3 Fluorometer (Thermo Fisher Scientific, Q32850), and the integrity of the genomic DNA was validated using Agilent 4200 TapeStation (Agilent ...
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bioRxiv - Biochemistry 2021Quote: Hsc70cb cDNA was amplified using PCR and inserted into the pProEX-HTA protein expression vector using 5’ SacI and 3’ SpeI restriction enzyme sites (Invitrogen, Carlsbad, CA, USA). Two Drosophila NBD constructs (with or without a stop codon ...
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bioRxiv - Neuroscience 2020Quote: ... blocked for 1 hour in 5 % normal donkey serum in 3% PBST and then incubated in chicken anti-GFP (Invitrogen, A10262, 1:1000) overnight at 4°C ...
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bioRxiv - Pathology 2021Quote: ... or LGALS1 siRNA (Eurofins Genomics; Ebersberg, Germany) with the sense sequence 5’-[UUGCUGUUGCACACGAUG-GUGUUGG]-3’ the following day using Lipofectamine® RNAiMAX (Thermo Fisher Inc) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Eluted RNA from each positive sample was used as template to synthesize cDNA using primer MBTuni-12 (5’-ACG CGT GAT CAG CRA AAG CAG G-3’) and Superscript III First Strand Synthesis SuperMix (Invitrogen, Carlsbad, CA, USA), following the manufacturer instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... or 72 hours with one of two different oligos (siNF-κB 5’-ACAGUAGGAAGAUCUCAUC-3’ and sip65 5’-UUUACGUUUUCUCCUCAAUC-3’) to the p65 subunit of NF-κB at a concentration of 100 nM using Oligofectamine (Life Technologies, Benicia, CA). After transfection ...
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bioRxiv - Molecular Biology 2022Quote: Human hPSCs cultured on MEFs were harvested using collagenase IV as big aggregates and settled 3 times in washing media (DMEM [Thermo Fisher Scientific], 5% Newborn Calf Serum [Sigma], 1X Penicillin & Streptomycin [Thermo Fisher Scientific]), then strained by an 80μm strainer ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Total RNA was prepared from tissue samples (3-5 mg) or cell pellets (2.5×105 cells) using the Ambion PureLink RNA minikit (Life Technologies, Carlsbad, CA, USA) as directed by the supplier ...
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bioRxiv - Microbiology 2022Quote: ... and Viral genomic terminal sequences were determined using commercial 5’ and 3’ RACE (rapid amplification of cDNA ends) kits (Invitrogen, Waltham, MA, USA). The PCR products were gel-purified using the Gel Extraction Kit (OMEGA Bio-Tec Inc. ...
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bioRxiv - Developmental Biology 2022Quote: ... were equilibrated in 3 ml of IVC1 medium in an incubator with a humidified atmosphere of 5% CO2 at 37 °C (Thermo Scientific, Heracell 240i) for at least 12 h prior to 3E-uterus embryo culture ...
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bioRxiv - Microbiology 2021Quote: ... were mixed with 1 pmol of AS primer 1 (5’-TGGGTGGTACTTAAGTTCGG-3’: complementary to nts 476-495 of A3G RNA) labeled with Vic (Life Technologies SAS, France). The mixture was heated at 90°C for 2 min and placed on ice for 2 min ...
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bioRxiv - Neuroscience 2021Quote: ... sections were washed in PBS 3 times for 5 minutes and mounted with ProLong Gold Antifade Mountant (#P36930, Thermo Fisher Scientific, Waltham, MA). Histological evaluation was performed single blinded ...
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bioRxiv - Microbiology 2020Quote: Treated and untreated biofilm-containing pegs after a 24-hour incubation in MMBC-3 were subjected to microscopic imaging after staining with 5 μM of Syto®9 green-fluorescent nucleic acid stain (Invitrogen, ThermoFisher Scientific) diluted in PBS ...