Labshake search
Citations for Thermo Fisher :
5401 - 5450 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2019Quote: ... the membrane was incubated in 5 ml TBST with secondary antibody (Fisher Scientific, cat #10751195) in the dark for 45-60 min ...
-
bioRxiv - Cell Biology 2020Quote: ... Human iPSC lines were maintained in 5% CO2 environment in Essential 8 Medium (Thermo Fisher). For cardiomyocyte differentiation ...
-
bioRxiv - Cell Biology 2020Quote: ... Cellular ROS measurements were obtained using H2-DCFDA flow cytometric dye (5 μM) (Thermo Fisher). For intracellular staining the cells were incubated for 4 h with the protein transport inhibitor GolgiPlug (BD Biosciences) ...
-
bioRxiv - Cell Biology 2020Quote: ... Positive clones were selected by incubation in medium containing 5 μg/ml blasticidin S (Invitrogen) and 400 μg/ml zeocin (Invitrogen ...
-
bioRxiv - Cell Biology 2020Quote: ... Stealth siRNAs were transfected at a final concentration of 5 nM using Lipofectamine RNAiMax (Invitrogen). Both siRNAs and RNAiMax were diluted in medium without serum ...
-
bioRxiv - Cell Biology 2020Quote: ... N2B27 1-5% KSR base media was supplemented with 1x NEAA (ThermoFisher Scientific 11140-050); 10 ng/mL recombinant human LIF (hLIF ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were incubated in 1 mL solution containing 5 μL Vybrant DiO (Molecular Probes, V22886) or Vybrant DiD (Molecular Probes ...
-
bioRxiv - Cell Biology 2020Quote: ... and imaged at 37°C and 5% CO2 in medium supplemented with 25mM HEPES (Thermofisher) and 1% PenStrep (Thermofisher) ...
-
bioRxiv - Genetics 2021Quote: ... followed by 2x 5 min washes in PBS-T0.1% before mounting in Prolong Diamond (Invitrogen). Slides were left to cure overnight at RT before sealing with nail polish.
-
bioRxiv - Genetics 2021Quote: ... 5 μm sections were cut using an automatic microtome (HM 355S, Thermo Scientific Kalamazoo, MI) and mounted onto positively charged slides (Superfrost Plus Microscope Slides ...
-
bioRxiv - Developmental Biology 2021Quote: ... containing 5% FBS and 1% MEM non-essential amino acids (NEAA) solution (Thermo Fisher Scientific) in a 96-well clear flat bottom microplate (Corning) ...
-
bioRxiv - Molecular Biology 2020Quote: ... All reactions were stopped with 5 μL Gel Loading Buffer II (Life Technologies, Cat # AM8547). Digested DNA was incubated at 75°C for 5 min before running on 4% (Nt.BspQI-digested samples ...
-
bioRxiv - Molecular Biology 2020Quote: ... and 5 min incubation (at 37 °C) of Hoechst dye (Life Technologies; 1:15,000 dilution) in warm PBS ...
-
bioRxiv - Neuroscience 2021Quote: ... with 5 μg primary antibody and 50 uL Pierce Protein G magnetic beads (Thermo Scientific). The primary antibodies used were rabbit anti-EMX2 (HPA065294 ...
-
bioRxiv - Microbiology 2020Quote: ... The cells were released from the flask using 5 mL of 0.05% trypsin-EDTA (Gibco), followed by washing three times with PBS (Gibco ...
-
bioRxiv - Microbiology 2019Quote: ... manY mRNA transcripts were incubated at 90 °C for 5 minutes in alkaline buffer (Ambion) to generate the alkaline ladder ...
-
bioRxiv - Molecular Biology 2021Quote: ... by incubation for 5 minutes at 37°C with DPBS-EDTA 0.5mM pH 8.00 (Invitrogen, Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2021Quote: Total RNA (2-5 μg) was resolved on a 15 % TBE-Urea gel (Invitrogen EC6885BOX). RNA of size between 17-25 nt was excised from the gel and extracted in 0.3 M NaCl overnight at 25°C ...
-
bioRxiv - Microbiology 2021Quote: ... qPCR was performed on QuantStudio 3 and 5 Real-Time PCR Systems (Thermo Fisher Scientific) based on the following cycling parameters ...
-
bioRxiv - Immunology 2020Quote: ... An additional 50 µL of 5× eBioscience Cell Stimulation Cocktail with protein transporter inhibitor (ThermoFisher) was added and cells were treated for an additional 4 hr at 37 °C.
-
bioRxiv - Developmental Biology 2021Quote: ... Anesthetized fish were injected intraperitoneally with 5 µl of 1 mg/mL EdU (Thermo Fisher) in sterile PBS ...
-
bioRxiv - Genomics 2020Quote: ... 5 µg antibody was coupled to 50 µl Protein A or Protein G Dynabeads (Invitrogen). The following antibodies were used ...
-
bioRxiv - Developmental Biology 2020Quote: ... dissected embryos were incubated with 1 mL of 5 µM LysoTracker Red DND-99 (Invitrogen) in HBSS for 45 min at 37°C ...
-
bioRxiv - Developmental Biology 2020Quote: ... Nuclei labelling was performed by incubating cells with DAPI for 5 min (Life technologies D1306) and Alexa Fluor 568 Phalloidin (1/600 ...
-
bioRxiv - Developmental Biology 2020Quote: ... Tissues were sliced at 5 μm using a HM335E microtome (Thermo Fisher Scientific, Waltham, Massachusetts). After deparaffinization ...
-
bioRxiv - Developmental Biology 2020Quote: ... Ovaries from ∼2-5 flies were dissected in Schneider’s Drosophila media (ThermoFisher SKU #21720-001) that was also used for ex vivo culturing ...
-
bioRxiv - Physiology 2022Quote: 5 μl of PowerUp SYBR Green master mix (Applied Biosystems, A25742, Foster City, CA, USA), 3 μl of primer mix and 2 μl of cDNA were added in duplo in a 96 wells plate ...
-
bioRxiv - Molecular Biology 2022Quote: ... into a 50 ml centrifuge tube filled with 5 ml fetal bovine serum (ThermoFisher Scientific), washed through with Dulbecco’s phosphate-buffered saline (ThermoFisher Scientific) ...
-
bioRxiv - Immunology 2022Quote: ... supplemented with 5% heat inactivated fetal bovine serum (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), 1% Penicillin-Streptomycin (Gibco ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells across the entire chip were fluorescently labeled by loading 5 μM SYTO 24 (Invitrogen) to both the sample and stimulation channels and incubate for 15 min ...
-
bioRxiv - Cancer Biology 2022Quote: All cell lines were grown at 37°C with 5% CO2 in advanced RPMI (Invitrogen) supplemented with fetal bovine serum (Tet tested ...
-
bioRxiv - Biophysics 2021Quote: ... The membrane was then coated with Rat-tail collagen I (5 μg cm-2, Gibco) for 4 hours and rinses with PBS ...
-
bioRxiv - Neuroscience 2021Quote: ... and 5% (by volume) fluorescent bead solution (6 μm FocalCheck Microspheres; part no. F14807; ThermoFisher), prepared using deionized water ...
-
bioRxiv - Neuroscience 2021Quote: ... coated with 0.1% Poly-L- ornithine and Laminin (5 ug/mL; Thermo Fisher Scientific, 23017015). On DIV 5 ...
-
bioRxiv - Genomics 2020Quote: ... single-cell suspensions were stained with 5 uM VybrantTM DyeCycleTM Violet Stain (Thermo Fisher Scientific) at 37°C for five minutes right before adding the C1 suspension buffer.
-
bioRxiv - Neuroscience 2021Quote: ... They were maintained within 37°C incubators at 5% CO2 in IMDM (Invitrogen, 124400-61) supplemented with 9.09% fetal bovine serum (50 mL added to 500 mL IMDM ...
-
bioRxiv - Neuroscience 2021Quote: ... 5 μM of the fluorescent dye Alexa 594 or 0.02% TMR biocytin (Thermo Fisher Scientific) was included in the intracellular solution to visualise basal dendrites ...
-
bioRxiv - Neuroscience 2021Quote: ... and a PepMap HPLC trap column (C18, 5 µm, 100 A; Thermo Fisher Scientific, U.S.A.) for loading samples ...
-
bioRxiv - Neuroscience 2021Quote: ... 5 μl of tracer cocktail (10 mg/ml EZ-Link NHS-Biotin (Thermo Fisher: 20217) and 10 kDa Dextran Alexa Fluor 488 (Thermo Fisher ...
-
bioRxiv - Molecular Biology 2020Quote: ... the total RNA was fragmented at 70 °C for 5-min (RNA fragmentation reagents; ThermoFisher), and then precipitated with 3 M sodium acetate ...
-
bioRxiv - Molecular Biology 2020Quote: ... Libraries were prepared by repairing 5’-OH of fragmented RNAs by Polynucleotide Kinase (Thermo scientific), followed by 3’and 5’adaptor ligation as described previously (Quarato et al. ...
-
bioRxiv - Molecular Biology 2021Quote: ... together with 5 μl of washed protein G-bound paramagnetic Dynabeads (Life Technologies cat#10003D). Antibody binding of RBP complexes was allowed to proceed at 4°C for the times indicated ...
-
bioRxiv - Molecular Biology 2021Quote: ... NSCs were counted and seeded in 5 mL D1 medium (EuroMed-N, 0.5% N2 (Invitrogen), 1% B27 (Invitrogen) ...
-
bioRxiv - Molecular Biology 2020Quote: ... in refreshed medium supplemented with 5% (V/V) exosome-depleted fetal bovine serum (A2720801, Gibco) for 24 h ...
-
bioRxiv - Molecular Biology 2019Quote: ... Lysate was incubated with 5 mg of the antibody (8WG16) or irrelevant rabbit IgG (Invitrogen). Library construction and Illumina sequencing were performed using Illumina HiSeq SE50 platform at BGI.
-
bioRxiv - Molecular Biology 2019Quote: Cells were pulse-labeled with the thymidine analog Bromodeoxyuridine / 5-bromo-2’-deoxyuridine (BrdU; ThermoFisher) for 2 hr (50mM ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were cultured at 37°C in 5% CO2 humidified atmosphere incubaters using DMEM (Gibco) culture medium supplemented with 10% foetal bovine serum and 1% penicillin-streptomycin ...
-
bioRxiv - Cell Biology 2022Quote: ... and FITC-conjugated anti-CD146 antibodies (5 μg/ml, OJ79c clone, mouse IgG1; ThermoFisher Scientific) in dark for 45 mins at 4°C ...
-
bioRxiv - Cell Biology 2022Quote: ... and FITC-conjugated anti-CD146 antibodies (5 μg/ml, OJ79c clone, mouse IgG1; ThermoFisher Scientific) in dark for 45 mins at 4 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... The frozen block was cut in 5-μm-thick using Cryostar NX70 (Thermo Fisher Scientific) and fixed in 10% formalin in 0.1 M phosphate buffer pH7.4 for 10 min at room temperature ...