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Citations for Thermo Fisher :
5451 - 5500 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The expression plasmid was transformed into BL21 Star DE3 competent cells (ThermoFisher, Cat. No. C601003). Colonies were picked for overnight growth in TB+25ug/mL kanamycin at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... The construct was synthesized and cloned commercially into pcDNA3.4-TOPO plasmid (Life Technologies B.V. Europe). SARS-CoV-2 Spike RBD 319-541 recombinant protein (OBA0101 ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 µg of plasmid pcDNA 3.1/RBD was linearized with BamHI enzyme (Thermo Fisher Scientific), precipitated ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were transfected with these plasmids (two per gene) using Lipofectamine 2000 (Thermo Fisher Scientific). 24 h post-transfection ...
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bioRxiv - Immunology 2023Quote: ... After co-transfection of the heavy and light expressing plasmid DNA into HEK293T cells (Invitrogen), the recombinant monoclonal antibodies were purified from the supernatant with protein G beads (GE Healthcare).
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bioRxiv - Immunology 2023Quote: ... 12 μg plasmid of each chain were mixed into 3 ml Opti-MEM (Gibco 31985070), followed by adding 24 μl FectoPRO transfection reagent (116-040 ...
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bioRxiv - Physiology 2023Quote: Plasmids were transiently transfected into HEK293T cells using Lipofectamine LTX transfection reagent (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... The cells were transfected with linearized HYBMTV plasmid using Lipofectamine 3000 (Invitrogen, ThermoFisher Scientific, USA) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... plasmids were isolated from these populations of desiccation survivors with minipreps (Invitrogen, Cat #K2100-01).
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bioRxiv - Molecular Biology 2023Quote: ... Briefly sORF-specific template plasmids were created by AccuPrime Supermix PCR (Thermo Fisher Scientific, 12344040) using the pPB_EF1_MCS-EGFP_IRES_Puro as a PCR template and utilising primers with the respective knockin-overhangs (ordered from Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... FD1014)-digested px330A-1x2_ PITCh_sgRNA plasmid using the T4 DNA ligase kit (Thermo Fisher Scientific, EL0016). For SNHG1_lnc_47 ...
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bioRxiv - Physiology 2023Quote: ... The cloned plasmid product was sequenced (Sanger method) using the Sanger Sequencing Kit (Applied Biosystems). For transient expression ...
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bioRxiv - Developmental Biology 2023Quote: ... Capped mRNAs were transcribed from linearized plasmids using SP6 or T3 mMessanger Machine Kit (Ambion), according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... The plasmids were then individually transfected to K562 cells via the Neon Transfection System (Invitrogen). Each group was transfected with 2 μg of DNA per 2 × 106 cells ...
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bioRxiv - Microbiology 2023Quote: ... The cells were transfected with linearized HYBMTV plasmid using Lipofectamine 3000 (Invitrogen, ThermoFisher Scientific, USA) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Luciferase reporter and plasmids encoding each gene were transfected into 293FT cells (Thermo Fisher Scientific) using Polyethylenimine Max (molecular weight [MW] ...
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bioRxiv - Microbiology 2023Quote: ... Expi293 cells were transfected with S-6P-NanoLuc expression plasmids using ExpiFectamine 293 (ThermoFisher Scientific). Four days later ...
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bioRxiv - Genetics 2023Quote: Two micrograms of plasmid products carrying sgRNAs with Cas9 were transfected using Lipo2000 (Thermofisher, 11668019) into 0.7 × 104 MPCs cells ...
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bioRxiv - Microbiology 2023Quote: ... HEK293 cells were transfected with appropriate plasmids using either Lipofectamine 3000 (Thermo Fisher Scientific Inc.) or JetPrime (Polyplus Transfection Inc. ...
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bioRxiv - Developmental Biology 2022Quote: ... The sgRNA was transcribed from the resulting plasmid with the MEGAshortscript T7 Kit (Life Technologies) and purified with the MEGAclear Kit (Life Technologies) ...
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bioRxiv - Biochemistry 2022Quote: ... Transfection of plasmids into cells was performed using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s protocols.
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bioRxiv - Cell Biology 2023Quote: ... Plasmids and their negative controls were transfected with RNAiMAX and Lipofectamine 2000 (Thermo Fisher Scientific), respectively ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were transiently transfected with the plasmid carrying the appropriate sgRNA using Lipofectamine 3000 (ThermoFisher) following manufacturer’s directions ...
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bioRxiv - Cancer Biology 2022Quote: ... or PM-GFP plasmids using Neon Transfection System 10 μL Kit (Thermo Fisher Scientific, #MPK1025) following the manufacturer’s protocol ...
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Quantitative Comparison of Presenilin Protein Expression Reveals Greater Activity of PS2-γ-SecretasebioRxiv - Cell Biology 2023Quote: ... 1.25 µg each of the two guide plasmids were prepared using Lipofectamine 3000 (Invitrogen L3000015) and cells transfected as per manufacturer instructions ...
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bioRxiv - Bioengineering 2023Quote: ... both plasmid and genetic constructs were digested with NcoI and BamHI restriction enzymes (FastDigest, Thermofisher), inactivated (5 min at 65 °C) ...
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bioRxiv - Bioengineering 2023Quote: ... Plasmids encoding fluorescent proteins were generally cloned into a modified pcDNA3.1 backbone (Thermo Fisher #87020b). Plasmid maps are available in Supporting Information 1 ...
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bioRxiv - Cell Biology 2023Quote: ... Then the cells were transiently transfected with the plasmid using Lipofectamine 3000 reagents (Invitrogen, L3000). After 36 h ...
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bioRxiv - Developmental Biology 2023Quote: ... gRNA/tracrRNA and Lipofectamine/plasmid were diluted separately in Opti-MEM (Thermo Fisher Scientific, 31985070), mixed together ...
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bioRxiv - Biophysics 2023Quote: ... Cells were transiently transfected with 400ng plasmid DNA prepared in OPTI-MEM Reduced Serum (Gibco), using Lipofectamine LTX with Plus-Reagent (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: Top10 cells were used for the amplification of recombinant plasmid DNA (Invitrogen Corp., Carlsbad, CA). The coding information of Nanobody molecule A was cloned into the multiple cloning site of a P ...
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bioRxiv - Systems Biology 2024Quote: ... at the 80% confluency and next day transfected with 0.5 µg plasmid and Lipofectamine (Invitrogen) at the ratio of 1:6 ...
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bioRxiv - Molecular Biology 2024Quote: ... The gTBE plasmids were linearized by the FastDigest KpnI restriction enzyme (Catalog# FD0524, Thermo Fisher), purified using Gel Extraction Kit (Catalog# D2500-03 ...
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bioRxiv - Plant Biology 2023Quote: ... The required plasmid DNA was added to 14 mL round-bottom culture tubes (Thermofisher, #150268). 300 uL of protoplasts and 300 µL of PEG solution (PEG 4000 (40% w/v) ...
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bioRxiv - Physiology 2024Quote: ... and transfected with the desired plasmid (0.5 µg per well) using Lipofectamine 3000 (Thermo Fisher) and the manufacturer’s procedure ...
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bioRxiv - Microbiology 2023Quote: ... 10 μg of each infectious clone plasmid was linearized overnight with NotI restriction endonuclease (Invitrogen), purified by phenol:chloroform extraction and ethanol precipitation ...
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bioRxiv - Biochemistry 2023Quote: ... Both constructs were synthesized commercially and cloned into pcDNA3.4-TOPO plasmid (Life Technologies B.V. Europe) that included a Tyr-Pho signal peptide and an N-terminal hexahistidine (6xHis ...
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bioRxiv - Cancer Biology 2023Quote: Plasmid DNA was amplified in Subcloning Efficiency DH5α competent cells (Invitrogen, Massachusetts, USA; # 18265-017) and extracted using QIAprep Spin Miniprep Kit (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: Plasmids encoding ps3G124.ex17 or psK27.ex17 were transfected at 200 ng in Optimem (Gibco) for 4 hours ...
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bioRxiv - Biophysics 2023Quote: ... Plasmid transfections were performed using Lipofectamine™ LTX with Plus reagent™ (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Renilla luciferase and empty vector plasmid mixture was added to Lipofectamine 2000 (Thermo Fisher Scientific) and 50 μL of Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were cotransfected with the following plasmids using 293fectin transfection reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions at a 3:7 ratio (0.3 µg and 0.7 µg) ...
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bioRxiv - Cancer Biology 2023Quote: ... for c-NHEJ assay) plasmids using the Lipofectamine 3000 Transfection Reagent (cat. no. L3000001, Invitrogen). After 8 h ...
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bioRxiv - Synthetic Biology 2023Quote: ... a pHR plasmid encoding the ZO-1ΔABS sequence was digested using Ajil (BmgBI) from ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... these parts were transferred into the pMpGWB102-GAL4DBD plasmid (54) via LR Clonase II (Invitrogen) from the entry vectors ...
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bioRxiv - Synthetic Biology 2023Quote: ... plasmids or synthetic DNA fragments was performed using Phusion High Fidelity Polymerase (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid DNA mix was diluted in 350 µl serum-free Opti-MEM media (Gibco, #31985070). In a separate reaction mix ...
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bioRxiv - Biochemistry 2024Quote: ... The Flp-In Core System included the pOG44 and pcDNA5/FRT plasmids (Thermo Fisher Scientific). These plasmids enable stable expression of a cDNA under control of the CMV promoter through integration into a host genome via Flp recombinase-mediated DNA recombination at an FRT site ...
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bioRxiv - Cancer Biology 2023Quote: ... and 50 ng of PGL4 (hRluc-CMV) plasmid using lipofectamine-3000 as transfection reagent (Invitrogen). For RU486 treatment ...
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bioRxiv - Molecular Biology 2024Quote: ... The DNA plasmid and oligonucleotides transfection were performed using Lipofectamine3000 reagent (Invitrogen, Carlsbad, CA, USA) and Opti-MEM Reduced-Serum medium (Invitrogen ...