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Citations for Thermo Fisher :
5301 - 5350 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... then transfected with 10ug plasmid DNA and 20 uL lipofectamine 2000 reagent (Invitrogen Life Technologies). Five hours after transfection ...
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bioRxiv - Immunology 2022Quote: ... The blunt ended plasmid was then ligated using T4 DNA polymerase (Thermo Fisher, Walthman, MA). NFAT-luciferase and AP-1 luciferase were previously described(41) ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmid DNA constructs were confirmed by sequencing on the ABI 3130xl capillary machine (Applied Biosystems) and PacBio RSII (Pacific Biosciences).
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bioRxiv - Neuroscience 2022Quote: ... then transfected with 10ug plasmid DNA and 20 uL lipofectamine 2000 reagent (Invitrogen Life Technologies). Five hours after transfection ...
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bioRxiv - Systems Biology 2022Quote: ... the JM109 strain was transformed with 1 μg of the pBlueScript II SK+ plasmid (Invitrogen), which harbors an ampicillin resistance gene as a selection marker ...
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bioRxiv - Microbiology 2022Quote: ... Cells (1×105) were transfected into HEK293 with 1µg plasmid DNA using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The vectors from positive colonies were extracted with the GenJet Plasmid Miniprep kit (Thermo Scientific) and sequenced before transformation into Agrobacterium tumefaciens EHA105 competent cells.
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bioRxiv - Neuroscience 2022Quote: ... DNA plasmids were diluted in 25 μl Opti-MEM I Reduced Serum Medium (Gibco; 31985062), then 0.25 μl PLUS reagent was added ...
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bioRxiv - Molecular Biology 2022Quote: ... The plasmid DNA was transfected by using Lipofectamine™ 2000 transfection reagent (ThermoFisher Scientific Inc.). In brief ...
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bioRxiv - Molecular Biology 2022Quote: ... 500-1000 ng of plasmid was transfected using Lipofectamine 3000 transfection reagent (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then transfected with 0.5μg of expression plasmid using lipofectamine-2000 reagent (11668019, Invitrogen) or lipofectamine-3000 (L3000015 ...
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bioRxiv - Biochemistry 2022Quote: ... 5 µg of plasmid was added to 2.5 mL Opti-MEM Reduced-Serum Medium (Gibco). 15 mg of sterile PEI was added to 2.5 mL Opti-MEM Reduced-Serum Medium ...
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bioRxiv - Microbiology 2022Quote: All plasmid derivatives were checked by colony PCR using DreamTaq Green PCR Master Mix (ThermoFisher), restriction patterns and eventually by Sanger sequencing.
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bioRxiv - Biochemistry 2022Quote: ... and the cells transfected with 100 ng Plasmid DNA per well using Lipofectamine 3000 (ThermoFisher) as described by the manufacturer ...
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bioRxiv - Bioengineering 2022Quote: ... and 133ng of PUFa-DRP plasmid using 1.5 µl Lipofectamine 3000 (Invitrogen, ThermoFisher Scientific #L3000075). For 53BP1-DRP screen ...
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bioRxiv - Bioengineering 2022Quote: ... and 133ng of PUFa-DRP plasmid using 1.5 µl Lipofectamine 3000 (Invitrogen, ThermoFisher Scientific #L3000075). For 53BP1-DRP screen ...
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bioRxiv - Synthetic Biology 2022Quote: ... hiPSCs were reverse transfected with plasmids using Lipofectamine Stem transfection reagent (STEM00001, Thermo Fisher Scientific) and Opti-MEM (31985088 ...
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bioRxiv - Cell Biology 2022Quote: ... The University of Western Australia) packaging plasmid were co-transfected into HEK293FT cells (Thermo Fisher) using TransIT-Lenti transfection reagent (Mirus ...
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KIF24 controls the clustering of supernumerary centrosomes in pancreatic ductal adenocarcinoma cellsbioRxiv - Cell Biology 2022Quote: ... Plasmid transfection into Panc1 and Lenti-X 293T cells was performed using Lipofectamine 2000 (Invitrogen) and PEI Max (Polysciences ...
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bioRxiv - Immunology 2021Quote: ... the corresponding heavy and light chain plasmids were chemically co-transfected into ExpiCHO cells (Gibco) and transiently expressed for 9 days ...
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bioRxiv - Immunology 2020Quote: ... Proteins were expressed by transfection of plasmids into Expi293 cells using Expifectamine transfection reagent (ThermoFisher) in suspension at 37°C for 4-5 days ...
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bioRxiv - Plant Biology 2021Quote: ... Prepared plasmids were used to transform Escherichia coli MachI cells (Thermo Scientific, Waltham, MA, US), selected using 50 mg/L chloroamphenicol (MP Biomedicals Inc. ...
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bioRxiv - Molecular Biology 2020Quote: ... 40 μg of plasmids were transfected into HCT116 cells using Lipofectamine 2000 (Thermo Fisher Scientific), following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... and then transfected along with Flp recombinase expressing pOG44 plasmid using Lipofectamine transfection reagent (Invitrogen) as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Two micrograms of plasmid were digested with the FastDigest Pstl restriction enzyme (Thermo Fisher Scientific), and the 900 bp piece was gel purified using a gel DNA extraction kit (Zymo Research) ...
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bioRxiv - Molecular Biology 2020Quote: ... Each plasmid was co-transfected with ViraPower(tm) Lentiviral Packaging Mix (ThermoFisher Scientific, cat # K497500) into 293FT cells to make virus particles ...
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bioRxiv - Neuroscience 2021Quote: ... the mouse ephrin-B3 was cloned into pSecTag-B plasmid (Termo Fisher Scientific, Waltham, USA). Six repeats of the myc epitope were cloned at the carboxyl end of ephrin-B3 ...
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bioRxiv - Cancer Biology 2021Quote: ... or the pLightSwitch- hINSM1-3’UTR plasmid using Lipofectamine 2000 reagent (Invitrogen™, Cat #: 11668019) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The ISG15-like systems were cloned into an arabinose-inducible plasmid (pBAD, Thermo Fisher #V43001). The cloned vector was transformed into E ...
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bioRxiv - Evolutionary Biology 2022Quote: ... As a control we prepared GFP dsRNA from a pGreen Lantern plasmid (Thermo Fisher Scientific) using specific primers (Table S7).
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bioRxiv - Immunology 2022Quote: ... Sequence-verified plasmids were then used to transform TOP10 chemically competent bacterial cells (ThermoFisher; #C404010) and endotoxin-free plasmid stocks were prepared (Qiagen EndoFree Plasmid Maxi Kit ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 µg of plasmid were combined in 250 µl Opti-MEM (Thermo Fisher Scientific). After 30 minutes the mixture was added dropwise to the cells ...
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bioRxiv - Cell Biology 2022Quote: ... Stable cell lines were established by transfecting plasmids using Lipofectamine 2000 or Lipofectamine 3000 (Invitrogen), as per the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... by using the PureLinkTM Fast Low-Endotoxin Midi Plasmid Purification Kit (Thermo Fisher Scientific, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Cells were transfected for transient plasmid over expression with lipofectamine (ThermoFisher Scientific. Cat. Nº: L3000001) as previously described (42 ...
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bioRxiv - Biophysics 2019Quote: ... Plasmids transfections into the cells was done using the Neon® Transfection System (Invitrogen, Singapore) according to the manufacturer’s manual containing cell culture medium (DMEM + 10% FBS) ...
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bioRxiv - Microbiology 2019Quote: ... All ORFS were transferred between plasmids using Gateway LR Clonase II Enzyme Mix (ThermoFisher Scientific). All clones were transformed into A ...
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bioRxiv - Immunology 2019Quote: ... Plasmids were into transfected into 293T by using Lipofectamine 2000 reagent (Invitrogen, CAT: # 11668-027) 29 ...
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bioRxiv - Cancer Biology 2019Quote: ... The resulting plasmids were transfected into INS-1 cells using Lipofectamine 2000 (Thermo Fisher Scientific); a BD FACSAria II (BD Biosciences ...
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bioRxiv - Molecular Biology 2019Quote: ... and 2.5 μg of plasmid DNA was delivered into HeLa cells using Lipofectamine 2000 (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... These plasmid backbones are versions of the pcDNA3.1/Hygro(+) Mammalian Expression Vector (Thermo Fisher #V87020), modified by our laboratory in previous work (Addgene #138749 ...
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bioRxiv - Microbiology 2019Quote: ... 900 μg of plasmid in 25 ml of Opti-MEM transfection medium (Life Technologies, CA) was mixed with 5 ml of PEI-MAX (1.0 mg/ml ...
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bioRxiv - Plant Biology 2019Quote: ... we cloned the CRSH cDNA (without the stop codon) into the plasmid pDONR/Zeo (Invitrogen) (Masuda et al ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... The FBW2 genomic sequence containing the attB sites was recombined into pDONR 221 plasmids (Invitrogen) and then transferred into the binary vector pGWB415 (Nakagawa et al. ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... The Flag-NtAGO1 sequence containing the attB sites was recombined into pDONR 221 plasmids (Invitrogen) and then transferred into the binary vector pB2GW7 (Karimi et al. ...
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bioRxiv - Microbiology 2020Quote: PCR amplicons used in plasmid cloning were generated using Fusion high fidelity DNA polymerase (ThermoFIsher) or Platinum Taq DNA polymerase ...
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bioRxiv - Microbiology 2020Quote: ... All pBAD inducible plasmids generated in this work were derived from pBAD/His A (Invitrogen). pJNS12 is pBAD-gfp-linker-virB flanked by NcoI and HindIII restriction sites ...
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bioRxiv - Molecular Biology 2021Quote: ... or GFP CDS fragments were ligated into the linearized pYES2 shuttle plasmid (Thermo Fisher Scientific) and verified by sequencing ...
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bioRxiv - Physiology 2020Quote: ... The CaMPARI gene was cloned into pDONR-221 plasmid using BP clonase (Invitrogen, 11789-020) to generate PME-CaMPARI ...
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bioRxiv - Molecular Biology 2020Quote: ... were transfected with 0.5 μg of plasmid DNA and 2 μl TurboFect (Thermo Fisher Scientific). Following 2 hours incubation ...