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Citations for Thermo Fisher :
5401 - 5450 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... The Grx1-roGFP2 plasmid was introduced into cells the next day using Lipofectamine 2000 (Invitrogen) according to the protocol described previously for 36 hours [20] ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were transfected with Lipofectamine™ 3000 Transfection Reagent (L3000150, Thermo Fisher Scientific, Waltham, MA). To ensure AID-tagging on both alleles ...
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bioRxiv - Cell Biology 2022Quote: ... and transfected with Caveolin1-EGFP plasmid (gift of Richard Lundmark) using Lipofectamine3000 (Invitrogen, L3000-001). After 48 h the cells were washed with PBS (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... Harvest plasmids after cultivation were transfected into the cells using lipofectamine 3000 (Invitrogen; Carlsbad, CA) according to manufacture’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... The pCRISPR plasmid was digested with SapI and simultaneously dephosphorylated with alkaline phosphatase (FastAP; ThermoFisher). Guide RNA primer sets A (KS40 5’-ACCGACCACAGAAACTGCGACTAG -3’ and KS41 5’-AACCTAGTCGCAGTTTCTGTGGTC-3’ ...
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bioRxiv - Genomics 2022Quote: ... The double-stranded DNA sequences were then cloned into the pENTR/D-TOPO plasmid (Invitrogen).
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bioRxiv - Genetics 2022Quote: ... the plasmids were transfected into HEK293FT cells (Lipofectamine® LTX with PLUS™ reagent, Invitrogen). Cells were lysed 48hrs after transfection and total RNA extracted (Trizol Reagent) ...
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bioRxiv - Immunology 2022Quote: ... Wild-type and mutant plasmids were transformed into MAX Efficiency Stbl2 competent cells (ThermoFisher Scientific) for maxi-DNA preparations and each SCIV genome was sequenced to confirm its integrity ...
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bioRxiv - Genetics 2022Quote: ... along with the appropriate donor plasmid (400ng) using Lipofectamine™ Stem Transfection Reagent (Invitrogen, STEM00001) in E8 medium in one well of a 24-well plate ...
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bioRxiv - Plant Biology 2022Quote: Most plasmids used in this study were generated using Gateway Cloning Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... the plasmid was religated with the T4 DNA Ligase (Thermo Fisher Scientific, Waltham, MA, USA). All the plasmids used here were verified by DNA sequencing.
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bioRxiv - Microbiology 2022Quote: ... The plasmids were transiently transfected into Expi293F cells using ExpiFectamine 293 Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: All plasmids indicated were purified by either PureLinkTM HiPure Plamid Midiprep kit (K210004; ThermoFisher Scientific) or Plasmid Maxi kit (12162 ...
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bioRxiv - Microbiology 2022Quote: ... in which cDNA derived from A549 cells had been cloned within the pDEST22 plasmid (Invitrogen) for in frame expression downstream of the activation domain of Gal4 (Gal4-AD) ...
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bioRxiv - Molecular Biology 2023Quote: ... The amplified cDNA fragment was cloned into plasmid pCRII-TOPO by TA cloning (Life Technologies). The sequence of the 5’UTR cDNA was determined using primer EV1F and compared to the sequence of the infectious clone used to prepare passage 0 virus.
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bioRxiv - Molecular Biology 2023Quote: ... they were co-transfected with Cas9 and two sgRNAs plasmids using Lipofectamine 3000 (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... were mixed with 1 μg of DNA plasmids mix diluted in OptiMEM (Gibco™ #11058021) added to the neuronal culture ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and multiple transformations were pooled and midiprepped (Chargeswitch Pro Filter Plasmid Midi Kit, Invitrogen CS31104). The library was then cut using SbfI and EcoRI sites contained within the spacer ...
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bioRxiv - Microbiology 2022Quote: ... Four μg of pure plasmid DNA was transfected with 30 μl of Lipofectamine 2000 (Invitrogen) in a total volume of 1 ml serum-free OptiMem (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... each with an N-terminal (His)6 tag from plasmids generated by GeneArt (ThermoFisher Scientific). For each purification ...
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bioRxiv - Neuroscience 2022Quote: ... 1,500 ng of plasmids encoding hSyn-RMA and 3.0 μl of lipofectamine 2000 (Life Technologies) were used to transfect PC-12 following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... coli cells and purified using the GeneJET plasmid miniprep kit (Thermo Fisher Scientific, Sydney, Australia). Purified plasmid DNA was sequence verified by the Australian Genome Research Facility (Sydney ...
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bioRxiv - Molecular Biology 2022Quote: ... digested with HindIII and NotI and ligated into a linearized pcDNA5/FRT/TO plasmid (Thermofisher). All PCRs were performed using Kapa HiFi DNA polymerase (Roche) ...
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bioRxiv - Molecular Biology 2024Quote: ... The forward transfection method was used to deliver plasmids by using Lipofectamine 3000 (Invitrogen, L3000001) or Lipofectamine LTX (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: Cells transfected with 8-10 μg of plasmids were lysed in NP-40 buffer (ThermoFisher) supplemented with protease inhibitor cocktail (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... DNA plasmids were diluted in 25 μl Opti-MEM I Reduced Serum Medium (Gibco; 31985062), then 0.25 μl PLUS reagent was added ...
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bioRxiv - Developmental Biology 2024Quote: ... Cas9 mRNA was transcribed from linearized pCS2-Cas9 plasmid using the mMachine SP6 kit (Ambion). To generate mutants ...
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bioRxiv - Genomics 2024Quote: ... Successful integration of the dCas9-VP16(10x) plasmid was selected for using G418 (Thermo Scientific) at a concentration of 800 µg/mL for approximately 2 weeks ...
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bioRxiv - Biochemistry 2024Quote: ... The plasmid solution was diluted with Opti-MEM (reduced serum free medium, 31985-047, Gibco) to a volume of 2.08 ml ...
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bioRxiv - Cell Biology 2024Quote: Plasmids used in this study were generated using the Gateway recombination system (Thermo Fisher Scientific). The cDNA of each gene was cloned into the pDONOR221 vector and sequenced before being recombined into the destination vector downstream of the inducible metallothionein promoter (pMT ...
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bioRxiv - Molecular Biology 2024Quote: ... the cells were transfected with indicated plasmids or siSnd1 by Lipofectamine 3000 Transfection Kit (Invitrogen). Cells were treated with and without 0.25 μm PA for 12h - 24h before collection ...
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bioRxiv - Molecular Biology 2024Quote: ... and transfected with the HDR donor and the Cas9 plasmids using Neon electroporation device (Invitrogen) according to user guide manual (for suspension cells) ...
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bioRxiv - Microbiology 2024Quote: ... cells were transfected with 30 µg of viral glycoprotein expression plasmid using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... cells were transfected with 0.4 µg plasmid DNA per construct using lipofectamine 2000 (Life Technologies) according to the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.4 μg of each plasmid containing gRNA1 and gRNA2 using Lipofectamine 3,000 (Life Technologies, L3000008) following manufacturer instruction ...
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bioRxiv - Neuroscience 2024Quote: ... The miRNA plasmids used for knockdown studies were generated following manufacturer’s instructions (K493600; ThermoFisher Scientific). The control miRNA construct was provided in the kit ...
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bioRxiv - Genetics 2024Quote: ... The amplified product and pAX198 plasmids were digested using FastDigest Bpu1102I and FastDigest BstX1 (ThermoFisher). Desired DNA fragments from the digested pAX198 were isolated through a 1% agarose gel and subsequently purified using the Expin™ Gel SV kit (GeneAll) ...
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bioRxiv - Microbiology 2024Quote: ... equi were prepared using a recombinant plasmid from the TA cloning® kit (Invitrogen, USA). A 10-fold serial dilution of the plasmid (from an initial concentration of 0.5x108 copy number/μL ...
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bioRxiv - Microbiology 2024Quote: ... The GeneJET Endo-free Plasmid Maxiprep Kit (Cat. K0861, Thermo Fisher Scientific Baltics UAB, Lithuania) was used to isolate and purify plasmid DNA from bacterial cultures according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... 45 μg expression plasmid in 1.25 ml of Opti-MEM transfection medium (Life Technologies, CA) was mixed with 1.25 ml of OptiMEM containing 0.5 ml of PEI-MAX (1.0 mg/ml) ...
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bioRxiv - Cell Biology 2023Quote: CAD cells were transfected with plasmid DNA by electroporation using the Neon Transfection System (Invitrogen) and the Neon Transfection Kit (cat#MPK1096B ...
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bioRxiv - Biophysics 2024Quote: ... 75ng DNAJB8-mRuby3 plasmids were separately co-transfected into the cells using lipofectamine 2000 (Invitrogen), with each DNAJB8 construct plated in 6 replicate wells ...
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bioRxiv - Biochemistry 2024Quote: ... 0.8-1 µg/mL plasmid DNA was transfected into ExpiCHO-S cells (ThermoFisher Scientific #A29127) using the Max Titer protocol (ThermoFisher Scientific MAN0014337) ...
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bioRxiv - Cell Biology 2024Quote: ... they were transfected with 1 μg plasmid DNA and 2.5 μl LipofectamineTM 2000 (ThermoFisher #11668027) in a total of 1 ml Opti-MEMTM media (ThermoFisher #31985070) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Invitrogen™ PureLink™ Pro Quick96 Plasmid Purification Kit (K211004A) was purchased from Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Transient transfection of HEK293 cells by plasmid DNA was carried out by Lipofectamine 2000 (Invitrogen). The cells were replated 2 days after transfection and treated with 1 mg/mL G418 sulfate (EMD Chemicals Inc. ...
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bioRxiv - Immunology 2023Quote: ... 20 µg of Heavy chain and Light chain plasmid was diluted in 1mL OptiMEM(Gibco) and 120 µL polyethyleneimine (1 mg/mL PEI ...
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bioRxiv - Developmental Biology 2023Quote: ... We also subcloned this cassette into a pAc5.1/V5-His A plasmid (Invitrogen #V4110-20) by cutting the cassette out of pUASP-attb-Cry2-CTEV-mCherry using NotI and XbaI (NEB #R3189S and #R0145S ...
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bioRxiv - Cell Biology 2022Quote: ... A431 plasmid transfection was done by using Lipofectamine™ 3000 Transfection Reagent (Thermo Fisher Scientific) following the manufacture’s recommendations.
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bioRxiv - Biochemistry 2022Quote: Plasmids for bacterial expression were generated using the Gateway technology subcloning system (Thermo Fisher Scientific). The initial double-stranded DNA insert containing the cDNA that encodes for the protein of interest was obtained either synthetically manufactured (Integrated DNA Technologies ...