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Citations for Thermo Fisher :
5501 - 5550 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... plasmids or synthetic DNA fragments was performed using Phusion High Fidelity Polymerase (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... 1.2 μg of CAG Transposase plasmid and 3 μl of Lipofectamine 2000 CD (ThermoFisher Scientific) in 90 μl of OptiPro was injected in the dorsal aorta of 2.5-day-old embryos ...
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bioRxiv - Neuroscience 2024Quote: ... The resulting variant DBT’ was subcloned into the pDEST plasmid using the Gateway system (ThermoFisher). For mammalian expression ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa cells were transfected with plasmids by Lipofectamine LTX reagent with Plus reagent (Thermo Fisher) and Lipofectamine 2000 transfection reagent (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were transfected with the indicated plasmids and siRNAs using lipofectamine 2000 (Thermo-Fisher Scientific). Transfection was done with 50 ng of total plasmid (50 ng when only one plasmid was transfected or two times 25 ng for conditions with two plasmids) ...
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bioRxiv - Biochemistry 2024Quote: ... The quality and concentration of plasmid DNA were measured by NanoDrop one spectrophotometer (Thermo Scientific). Plasmids with various mutations were generated by PCR methods using Q5® Site-Directed Mutagenesis Kit (NEB ...
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bioRxiv - Biophysics 2024Quote: ... Solution A [200 µL of a mixture of plasmid and Opti-MEM (Thermo Fisher Scientific)] and solution B [200 µL mixture of PEI and Opti-MEM at a ratio of 1 ...
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bioRxiv - Cell Biology 2024Quote: ... 300ng of plasmid was transfected using Lipofectamine Stem Transfection Reagent (Thermo Fisher Scientific, Cat# STEM00001) in 150µl Opti MEM ...
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bioRxiv - Biochemistry 2024Quote: ... using 0.6 µg of each plasmid and 0.6 µl of Lipofectamine 2000 (Invitrogen, 11668-027). A transfection rate of 2-5% was seen the next day ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transiently transfected with purified plasmids using Lipofectamine 3000 transfection reagent (Thermo Fisher Scientific).
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bioRxiv - Immunology 2024Quote: The following additional plasmids were used in this study: pMK-PLEKHG3 was generated by Invitrogen GeneArt Gene Synthesis (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... and 150 ng of the ADAR2 overexpression plasmids were co-transfected with Lipofectamine 3000 (Invitrogen). Luciferase assays and evaluations of mRNA levels were performed as described above after 40 hours of transfection.
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bioRxiv - Microbiology 2024Quote: ... equi were prepared using a recombinant plasmid from the TA cloning® kit (Invitrogen, USA). A 10-fold serial dilution of the plasmid (from an initial concentration of 0.5×108 copy number/μL ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmids were constructed using Gibson Assembly EX Cloning Kit or T4 DNA ligase (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmids were transiently transfected into stably Cas9 expressing target cells using RNAiMax (Invitrogen, product #13778075). GFP positive cells were sorted by flow cytometry and sequenced by Sanger sequencing using the Mix2Seq kit (Eurofins).
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bioRxiv - Systems Biology 2024Quote: ... and 10µL cells and plasmid mix were electroporated using a 10µL pipet tip (Invitrogen #MPK1025) with the following settings ...
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bioRxiv - Microbiology 2024Quote: ... using PCR products from other plasmids or synthesized DNA fragments (GeneArtTM StringTM fragments, ThermoFisher, USA). The final GD donor plasmid included homology arms for the UL37-38 region ...
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bioRxiv - Immunology 2021Quote: ... T-cells were stimulated with Dynabeads Human T-Activator CD3/CD28 (ratio 1:2, Thermo Fisher Scientific) in the presence of PBMC-derived CECs (ratio 1:2 ...
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bioRxiv - Immunology 2021Quote: ... Protein secretion levels in culture supernatant were quantified via ELISA for human IgG (Invitrogen, 50-112-8849) and human BAFF (R&D Systems ...
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bioRxiv - Genetics 2021Quote: Immortalized Human Embryonic Kidney cells (HEK293T) were cultured in DMEM (high glucose, L-glutamine, sodium pyruvate; Gibco) supplemented with 10% Hyclone Fetal Bovine Serum (GE Life Sciences) ...
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bioRxiv - Molecular Biology 2021Quote: U2OS human osteosarcoma cells were obtained from ATCC and grown in Dulbecco’s Modified Eagle Medium (DMEM; Invitrogen) supplemented with 10% fetal bovine serum (FBS) ...
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The key features of SARS-CoV-2 leader and NSP1 required for viral escape of NSP1-mediated repressionbioRxiv - Molecular Biology 2021Quote: Human HEK293T cells were grown in Dulbecco’s modified Eagle’s medium with GlutaMAX™ supplement (DMEM+ GlutaMAX, GIBCO) with 10% FBS ...
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bioRxiv - Developmental Biology 2021Quote: ... were coated with 10 μg/ml recombinant human laminin 521 (BioLamina) diluted in DPBS (Thermo Fisher Scientific) for 3 hours at 37°C and washed with DPBS twice ...
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bioRxiv - Microbiology 2022Quote: ... was cultured in O+ human erythrocytes at 4% haematocrit in RPMI 1640 supplemented with 0.25 % albumax (Invitrogen), 5% heat-inactivated human serum and 0.25% sodium bicarbonate in gassed chambers at 1% O2 ...
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bioRxiv - Immunology 2022Quote: ... soluble extracellular domain (ECD) of human CD58 was produced either in Freestyle 293F suspension cells (Thermo Fisher) or adherent HEK 293T cells ...
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bioRxiv - Neuroscience 2021Quote: ... Human recombinant full length wild type Flag-LRRK2 (#A15198) and Flag-LRRK2[G2019S] (#A15201) were from ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: Human iPSCs (male WTC11 background77) were cultured in Essential 8 (E8) Medium (ThermoFisher Scientific cat. no. A1517001) on BioLite Cell Culture Treated Dishes (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... and Myc-tagged human Insig1 (NM_005542) were prepared by subcloning PCR products into the pcDNA3 vector (Invitrogen). YFP-tagged human RHBDL4 and YFP-human SREBP-1c-CFP were prepared by subcloning PCR products into the pCI-EYFP vector ...
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bioRxiv - Molecular Biology 2020Quote: ... primary Hs27 human fetal foreskin fibroblasts (ATCC Cat#CRL-1634) all maintained in DMEM (Gibco Cat# 11995065) supplemented with 10% FBS and 1% antibiotic/antimycotic ...
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bioRxiv - Molecular Biology 2021Quote: ... we modified the default protocol of the Ion AmpliSeq Transcriptome Human Gene Expression kit (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Bioengineering 2022Quote: Human or mouse CDCs were transfected with small interfering RNAs against Drosha (Thermo Fisher HSS178992 or MSS274198) or a Medium GC Content Negative Control (Thermo Fisher ...
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bioRxiv - Bioengineering 2022Quote: Flp-In T-REx Human Embryonic Kidney (HEK) 293 cells were purchased from Thermo Scientific (catalog #R78007). Cells were cultured in a humidity-controlled incubator under standard culture conditions (37°C with 5% CO2 ...
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bioRxiv - Developmental Biology 2022Quote: ... Full-length human SAMD1 was produced by Cell & Molecular Technologies (CMT, Phillipsburg, NJ, now Invitrogen, Carlsbad, CA) in HEK293 cells ...
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bioRxiv - Genomics 2020Quote: ... isolated single cells were stained with mouse anti-human CD34 biotinylated antibody (clone 581, Thermo Fisher Scientific) diluted 5µL for 106 cells in FACS buffer (DPBS with 2% FBS and 1mM EDTA ...
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bioRxiv - Microbiology 2020Quote: ... TNFα using the ProcartaPlex high sensitivity 9-Plex Human Panel (EPXS090-12199-901, Thermofisher Scientific, Waltham, MA). Samples were measured using a Luminex MAGPIX instrument (Luminex Corporation ...
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bioRxiv - Microbiology 2020Quote: Human 293F cells were maintained at 37°C with 5% CO2 in FreeStyle 293 Expression Medium (ThermoFisher) supplemented with penicillin and streptomycin ...
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bioRxiv - Cancer Biology 2020Quote: Human Embryonic Kidney (HEK-293) cells were cultivated in DMEM (Gibco®, Thermo Scientific, Carlsbad, CA, USA) supplemented with 10% FBS,2mM glutamine and 1% penicillin/streptomycin.
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bioRxiv - Cancer Biology 2020Quote: Human Embryonic Kidney (HEK-293) cells were cultivated in DMEM (Gibco®, Thermo Scientific, Carlsbad, CA, USA) supplemented with 10% FBS,2mM glutamine and 1% penicillin/streptomycin.
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bioRxiv - Genetics 2020Quote: ... reference primers/probe (VIC, TaqMan™ Copy Number Reference Assay, human, RNase P, 4403326; ThermoFisher, Waltham, MA)] and applied to a X200™ Droplet Generator (1864002 ...
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bioRxiv - Neuroscience 2019Quote: ... Human synaptosomes were labeled with blue fluorescent 2.0 µm FluoSpheres™ Carboxylate-Modified Microspheres (Life technologies, F8824) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... After an overnight rest 20 μL of Dynabeads™ Human T-Activator CD3/CD28 (Thermo Fisher #1131D) were added per well and incubated for 24 hours ...
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bioRxiv - Bioengineering 2019Quote: ... 10x of mouse (for NIH 3T3 cells) or human (for HCT116 cells) Cot-1 DNA (Thermo Fisher Scientific Cat ...
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bioRxiv - Immunology 2019Quote: Human peripheral blood or umbilical cord blood was diluted with an equal volume of RPMI-1640 (Gibco), overlaid on Histopaque (Sigma) ...
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bioRxiv - Neuroscience 2019Quote: ... and 5 μg/mL human recombinant laminin 521 (BioLamina #LN521-02) in E8 basal medium (Gibco #A1517001) supplemented with 1% Penicillin/Streptomycin (Pen/Strep ...
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bioRxiv - Immunology 2019Quote: Cryopreserved T cells were thawed and activated same day with Human T-Expander CD3/CD28 Dynabeads (Gibco) at 3:1 beads:cell ratio in T cell media (AIMV supplemented with 5% FBS ...
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bioRxiv - Developmental Biology 2020Quote: Primary prenatal human microglia were MACS-purified as described above and labeled with DiI (Thermo Fisher, V22885) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Alexa Fluor conjugated secondary antibodies used: goat anti-human Alexa-488 (1:1000, Cat. # A11013, Life Technologies), donkey anti-mouse Alexa-594 (1:1000 ...
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bioRxiv - Systems Biology 2019Quote: Full-length cDNAs were obtained from the human ORFeome v5.1 entry clone collection (Thermo Fisher, Open Biosystems) and sequence verified ...
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bioRxiv - Immunology 2019Quote: ... Corresponding cDNAs were made by gene synthesis and codon-optimised for expression in human cells (Invitrogen, GeneArt). The ectodomains were flanked by unique NotI and AscI restriction enzyme sites and subcloned into an expression plasmid containing a high-scoring signal peptide [29] ...
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bioRxiv - Microbiology 2019Quote: ... transformed into MaV203 and used as a bait to screen a human embryonic brain cDNA library (Invitrogen). Media ...