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Citations for Thermo Fisher :
5301 - 5350 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... followed by an incubation of 2 h at RT in the presence of a species-specific Alexa Fluor A633 conjugated secondary antibodies (A21071, Thermo Fisher Scientific). After centrifugation and washings ...
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bioRxiv - Microbiology 2020Quote: ... Then all labelled cells were incubated for 2 h at RT in the presence of a species-specific A488 conjugated secondary antibodies (A21071, Thermo Fisher Scientific). After centrifugation and washing ...
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bioRxiv - Microbiology 2021Quote: ... immunostained with rabbit anti-O78 antibody followed by cross absorbed goat anti-rabbit AlexaFluor 594-conjugated IgG (H+L) (ThermoFisher A-11072). Membranes with fluorescently labeled samples were excised and mounted onto glass slides with ProLong Gold (ThermoFisher Scientific P36930) ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 h for cells or 2 h for tissues at room temperature and the nuclei were stained with Draq5 (1:1000, Thermo Fisher Scientific) for 40 min or 4′,6- diamidino-2-phenylindole (DAPI ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated with 10 µM EdU for 3 h and analyzed using Click-iTTM EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific). Cells were recorded with a BDTM LSRII (BD Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... sections were incubated at RT for 1 h in solution containing appropriate secondary antibodies conjugated to fluorescents: Alexa Fluor 488 (A11008, Invitrogen, Massachusetts, USA) and 555 (A21434 ...
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bioRxiv - Pathology 2021Quote: ... The digestion was carried out at room temperature for 1 h with gentle rotation and stopped by adding 15 μL SUPERase·In RNase Inhibitor (Thermo Fisher Scientific; AM2696). The digestion mixture was layered onto 0.4 ml of sucrose cushion (containing 40 mM Tris-HCl (pH 8) ...
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bioRxiv - Neuroscience 2020Quote: ... the blots were incubated for 1 hr with goat anti-rabbit HRP conjugated IgG (H+L) (1:1000; Thermo Scientific, Waltham, MA). PierceTM ECL Western blotting substrate (Thermo Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 2 h incubation with secondary antibody goat-anti-rabbit Alexa Fluor 488 (1:500, #A11008, Invitrogen-Thermo Fisher, MA, USA). Sections were transferred to glass slides and covered with Fluoromount (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 2 h incubation with secondary antibody goat-anti-rabbit Alexa Fluor 488 (1:500, #A11008, Invitrogen-Thermo Fisher, MA, USA). Sections were transferred to glass slides and covered with Fluoromount (Sigma-Aldrich ...
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bioRxiv - Physiology 2021Quote: ... Membrane damage was assessed using anti-IgG staining using an anti-rat antibody (IgG H+L AlexaFluor® 647 1:100, Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... cinerea germlings exposed to different concentrations NCR044.1 was measured every 30 min for 2 h using the ROS indicator dye 2′,7′-dichlorodihydrofluorescein diacetate (H2DCF-DA, Invitrogen, Carlsbad, CA). ROS levels were quantified by measuring the fluorescence in a SpectraMax® M3 spectrophotometer microplate reader (exc ...
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bioRxiv - Microbiology 2020Quote: ... To starve Chlamydia of tryptophan HeLa cells were incubated for 24 h in media containing 2 ng/ml recombinant human IFN-γ (Invitrogen: PHC4033) prior to infection ...
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bioRxiv - Molecular Biology 2021Quote: ... the samples were washed with PBS and incubated in the dark for 1 h at 4 °C with secondary antibody (Alexa Fluor-568-conjugated anti-mouse antibody, 1:750, Thermo Fisher Scientific). Finally ...
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bioRxiv - Microbiology 2020Quote: ... followed by 1 h of incubation with Alexa Fluor 488-labeled goat anti-mouse IgG (2.5 µg/ml; Thermo Fisher Scientific, Poland). Actin filaments was stained using phalloidin coupled with Alexa Fluor 633 (0.2 U/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA (500 ng) was used for the synthesis of cDNA using the RevertAid H Minus First Strand cDNA Synthesis kit (Thermo Fisher Scientific) with random hexamer primers ...
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bioRxiv - Cancer Biology 2020Quote: ... Secondary antibodies for immunofluorescence were Alexa Fluor™ 488 Goat anti-Rabbit IgG (H+L) (Cat no. A11034, 1:500, ThermoFisher Scientific), Alexa Fluor™ 546 Goat anti-Mouse IgG (H+L ...
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bioRxiv - Cell Biology 2021Quote: ... Fresh tissue samples macroscopically identified as cancer by a pathologist with specialization in uropathology (H. Moch) were placed into sterile 50-ml conical tubes containing transport media (RPMI (Gibco, Waltham, MA) with 10 % fetal calf serum (FCS ...
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Mitochondrial and metabolic remodeling in human skin fibroblasts in response to glucose availabilitybioRxiv - Cell Biology 2021Quote: ... LGm and OXPHOSm were plated in IBIDI μ-Slide 8 Well ibiTreat at a density of 6000 cells/well and after 24 h cells were incubated for 90 minutes with MitoTracker Red CMXRos (Molecular Probes, Invitrogen) at 100 nM ...
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bioRxiv - Cell Biology 2021Quote: ... heart sections were incubated with rabbit anti-TFEB (SC48784, 1:500 dilution) antibody for 1 h and subsequently Alexa Fluor 568 goat anti-rabbit (Invitrogen, A-21069) or Alexa Fluor 488 goat anti-mouse (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: 500 ng of RNA were reverse transcribed into cDNA using a RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Fisher Scientific) with random hexamer primers ...
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bioRxiv - Cell Biology 2020Quote: ... followed by incubation with respectively Alexa Fluor 555-conjugated goat anti-mouse IgG (H+L) (Thermo Fisher Scientific #A21422, diluted 1:400) and 488-conjugated goat anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2021Quote: ... droplets were broken promptly and barcoded beads with captured transcriptomes were reverse transcribed using Maxima H Minus Reverse Transcriptase (Thermo Fisher Scientific) at RT for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... Stefan Finke) and secondary antibody goat anti-rabbit IgG (H+L) conjugated with Alexa Fluor 488 (dilution 1:1000; Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... then incubated at room temperature for 1 h in Alexa Fluor™ 555 conjugated goat anti-mouse secondary antibody (Life Technologies, A21424) diluted to 2 μg/ml in PEMBALG ...
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bioRxiv - Cancer Biology 2020Quote: ... After 24 h, CCK-8 (Dojindo, Japan) was used to measure drug sensitivity at 450 nm using a microplate reader (Thermo Fisher, USA) after incubating at 37 °C for 1–2 h.
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bioRxiv - Cell Biology 2021Quote: ... and the cells were harvested 72 h after the infection of baculovirus geverated by the Bac-to-Bac system (Invitrogen, cat#10359016). For purification ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were washed 3 times for 10 min in PBS 1X + 0.1% Triton X-100 + 1% BSA and incubated for 6h with secondary antibodies at 4°C: goat anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody Alexa Fluor 568 (1/500, Invitrogen #A-11031), goat anti-rabbit IgG (H+L ...
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bioRxiv - Biochemistry 2022Quote: ... The protein-lipid mixtures were incubated for 1 h at +22 °C and then transferred into Slyde-A-Lyzer mini dialysis devices (Thermo Fisher Scientific) with 20 kDa MW cut-off or 10-20 µl dialysis buttons (Hampton Research ...
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bioRxiv - Biochemistry 2022Quote: ... and anti-Goat Mouse IgG (H+L) cross-adsorbed secondary antibody with Alexa Fluor 647 (Thermo Fisher Scientific, catalog number A-21235). The colocalized areas from YFP-Parkin and anti-Tom20 fluorescence were quantitated using Nikon NIS-Elements AR imaging software.
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bioRxiv - Bioengineering 2022Quote: ... they were washed twice with DPBS and incubated with goat anti-rabbit IgG (H + L)-Alexa Fluor 647 (1:1000, Invitrogen, Waltham, MA) for 1 h at room temperature ...
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bioRxiv - Pathology 2022Quote: ... The samples were cryosectioned with a thickness of 12 μm and stained using rapid chrome H&E staining kit (Thermo Scientific, 9990001).
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bioRxiv - Neuroscience 2022Quote: ... The primary antibody incubation was followed by 2 h incubation at room temperature with corresponding fluorescent secondary antibodies (1:500, Thermo Fisher Scientific). Nuclei were counterstained with DAPI (1:2000 in 0.1 M PB ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were resolved by electrophoresis at 150 V for 3 h at room temperature in Novex TBE-Urea Gels (EC6885BOX, Thermo Fisher Scientific) in 1x TBE buffer and cleaved and uncleaved products were imaged on ChemiDoc (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: ... Resulting peptides were separated during a 1 h gradient on C18 material using an Ultimate3000 rapid separation liquid chromatography system (Thermo Fisher Scientific) and subsequently injected in an online-coupled QExactive plus mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... This population was then treated with TMP (10 μM) and sorted 24 h later for YFP-positive cells with fluorescence into black clear-bottomed 96 well plates (Nunc, Roskilde, Denmark), with a single cell per well ...
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bioRxiv - Biochemistry 2022Quote: ... the supernatant was incubated at 4°C for 1 h with anti-BmAgo3 antibody23 conjugated to Dynabeads protein G (Thermo Fisher Scientific). The superparamagnetic beads were washed five times with wash buffer (30 mM HEPES-KOH ...
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bioRxiv - Neuroscience 2022Quote: ... and for 3 h with a 1:250 dilution of a goat anti-rabbit secondary antibody conjugated to DyLight 680 fluorophore (35569, Invitrogen, Waltham, Ma). Finally ...
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bioRxiv - Cancer Biology 2022Quote: ... The spatial transcriptomics slide was then stained with hematoxylin & eosin (H&E) and imaged with the EVOS M7000 Imaging system (Thermo Fisher Scientific). Tissue permeabilization and cDNA synthesis were then performed directly on the tissue section ...
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bioRxiv - Molecular Biology 2022Quote: ... 40U Murine RNase Inhibitor (Vazyme, R301-03), 1 μM Strand-Switching Primer (Nanopore, SQK-PCS109) and 200U Maxima H Minus Reverse Transcriptase (Thermo Fisher, EP0752), and then incubated at 90 min 42°C ...
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bioRxiv - Neuroscience 2022Quote: ... incubated with species-specific horseradish peroxidase–conjugated secondary antibody for 1 h at room temperature and developed using electrochemiluminescence (ECL) solution (Thermo Scientific Pierce).
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bioRxiv - Immunology 2022Quote: ... slides were incubated with a purified anti-CD11b/c (a list of the clones and suppliers of all mAbs used in the study in Supplemental Table 1) and the staining was revealed using a goat anti-mouse IgG (H+L)-AF488 (Invitrogen, Carlsbad, CA). After staining ...
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The structure of insulin granule core determines secretory capacity being reduced in type-2 diabetesbioRxiv - Physiology 2022Quote: ... INS-1 832/13 cells were seeded in six-well plates at a density of ∼5 x 105 cells for 24 h and then transfected by Lipofectamine® RNAiMAX Transfection Reagent (Invitrogen, USA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... and the secondary antibody reaction was performed using goat anti-mouse IgG (H+L) (Alexa Fluor Plus 555; Invitrogen, Waltham, MA, USA) at 1:200 dilution with 1% BSA-PBS for 40 min at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Fresh tissue samples were collected within 2 h of surgery and were sliced to 5 mm3 and immersed in 10 vol of RNALater (Ambion, Austin, TX). The tissue samples were stored at –80 °C ...
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bioRxiv - Microbiology 2022Quote: ... GTX135357) primary antibody for 16 h at 4 °C and detected with anti-rabbit-Alexa488 (1:200, A11008, Invitrogen, Carlsbad, CA, USA) secondary antibodies for 40 min at room temperature ...
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bioRxiv - Bioengineering 2022Quote: We prepared PFA-fixed lower brainstem tissue samples from 12-week-old WKY rats 24 h after introducing fluorescently labeled hydrogel conjugated with Alexa Fluor 594 C5 maleimide (A10256, Thermo Fisher Scientific) to their RVLM as described above ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed to remove the excess of primary antibody and incubated with the appropriate Alexa-Fluor secondary antibodies for 2 h at 25 °C (1:1000, Thermo Fisher Scientific). The excess of secondary antibody was washed ...
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bioRxiv - Immunology 2022Quote: ... with PBS-T followed by a 2 h incubation on a shaker at room temperature with goat anti-rabbit IgG-HRP (1:5000) (Thermo Fisher Scientific) in blocking buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... incubated with HRP-conjugated secondary antibodies at room temperature for 1 h and visualized using enhanced chemiluminescence substrate (SuperSignal™ West Dura Extended Duration Substrate #34076, Thermo Scientific).