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Citations for Thermo Fisher :
5151 - 5200 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Cells were then induced by the addition of 0.5 mM Isopropyl B-D-1-thiogalactopyranoside (IPTG, INVITROGEN Life Technologies) and further grown at 21°C for 16-18 h ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then induced by the addition of 0.5 mM Isopropyl B-D-1-thiogalactopyranoside (IPTG, INVITROGEN Life Technologies) and further grown at 21°C for 16-18 h ...
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bioRxiv - Cancer Biology 2021Quote: ... The U2AF65 isoform α and β were cloned in pENTR/D-TOPO cloning vector (Invitrogen, Cat. No. 45-0218), with PCR-based techniques ...
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bioRxiv - Cell Biology 2020Quote: ... Glucose uptake assay was measured as previously described using the fluorescent D-glucose analog 2NBDG (200 μM; Molecular Probes) [15] ...
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bioRxiv - Biochemistry 2021Quote: ... were backfilled with PBS (pH 7.4) to which 100 μM full-length D-serFS and 12.5 μM streptavidin (Life Technologies) had been added ...
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bioRxiv - Neuroscience 2020Quote: ... except they were plated and maintained using media that consisted of 1x DMEM (+4.5g/L D-glucose and L-Glutamine, Gibco), Pen-strep and 10% FBS ...
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bioRxiv - Microbiology 2022Quote: ... or in 2 ml liquid MSgg with 7-d-old tomato seedlings in a 6-well plate (Thermo Scientific). Each well contained one seedling ...
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bioRxiv - Developmental Biology 2020Quote: ... MpBELL3 (5.6kb) and MpBELL4 (5.8kb) were amplified and cloned into pENTR-D and subsequently recombined using LR-clonase II (Invitrogen) into pMpGWB104 (Addgene entry #68558 ...
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bioRxiv - Immunology 2020Quote: ... cat#11360070)] supplemented with 20% L929 conditioned medium or ‘MEF medium’ [4.5 g/liter D-glucose in DMEM with GlutaMAX (Gibco), 20% heat-inactivated FBS (Omega Scientific) ...
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bioRxiv - Immunology 2020Quote: ... BMDMs or mouse embryonic fibroblasts (MEF) were plated on coverslips with ‘BMDM medium’ [4.5 g/liter D-glucose in DMEM with GlutaMAX (Gibco), 20% heat-inactivated FBS (Omega Scientific) ...
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bioRxiv - Immunology 2020Quote: ... whereby the transfected population is selected for removal of HXGPRT and replacement with the complementation allele in 6-thioxanthine selection medium [177 µg/mL of 6-thioxanthine (TRC, cat# T385800) in 4.5 g/liter D-glucose in GlutaMAX DMEM (Gibco), with 1% dialyzed FBS (Omega Scientific ...
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bioRxiv - Cell Biology 2020Quote: Bone marrow cells were isolated and cultured for 7±9 d in RPMI-1640 medium (Gibco, ThermoFisher, Waltham, MA) supplemented with 10% low endotoxin defined FBS (Hyclone ...
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bioRxiv - Cell Biology 2020Quote: Bone marrow cells were isolated and cultured for 7±9 d in RPMI-1640 medium (Gibco, ThermoFisher, Waltham, MA) supplemented with 10% low endotoxin defined FBS (Hyclone ...
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bioRxiv - Neuroscience 2020Quote: ... Prior to transfection cells were brought into suspension by trypsinization and resuspension to 0.18 million cells/mL in growth medium (D-MEM, Gibco 10566016; supplemented with 10% Fetal Bovine Serum, Gibco 10270106 ...
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bioRxiv - Neuroscience 2020Quote: ... Prior to transfection cells were brought into suspension by trypsinization and resuspension to 0.18 million cells/mL in growth medium (D-MEM, Gibco 10566016 ...
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bioRxiv - Neuroscience 2020Quote: ... To visualize the vasculature Texas Red Dextran (2.5% w/v, 70,000 mw, 50μl, Life Technologies, catalog number D-1864) was injected intravenously ...
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bioRxiv - Cell Biology 2021Quote: ... Harvested PBMCs from the supernatants of co-cultures were collected and further stained for vitality (L/D Blue, Invitrogen) and the surface markers CD3 ...
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bioRxiv - Neuroscience 2021Quote: ... the cured PDMS was treated with oxygen plasma (30s, 100%, 7sccm) (Henniker Plasma) and coated with poly-D-Lysine 0.01% (PDL) (Gibco) for 15min and laminin (Sigma ...
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bioRxiv - Pathology 2020Quote: ... by mixing 100 μL of lysate with 100 μL of D-luciferin (0,1 mg/mL) supplemented with ATP (0,5 M) in flat bottom 96-well black plates (Thermo Scientific). Luciferase activity was measured for 1 min with f/stop = 1 and binning = 8 ...
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Sapogenin based self-assembly structures activating a non-apoptotic cell death via multiple pathwaysbioRxiv - Pharmacology and Toxicology 2021Quote: Microarray analyses were conducted using the human Clariom D Affymetrix platform following the manufacturer’s instructions (902915, Thermo Fisher Scientific). Briefly ...
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bioRxiv - Biochemistry 2021Quote: Prion-infected brain homogenate was prepared by homogenizing 200 brains from CD-1 mice terminally-infected with the RML prion strain in Dulbecco’s phosphate buffered saline (D-PBS; Gibco) to produce a pool of ~1 litre 10 % (w/v ...
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bioRxiv - Immunology 2021Quote: ... Vortexed beads were aliquoted into microcentrifuge tubes and incubated on an Invitrogen DynaMag-2 Magnet (ThermoFisher 12-321-D) for two minutes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and rinsed 3 times with Dulbecco’s phosphate buffer saline (D-PBS) (Thermo Fisher Scientific, Invitrogen #14190169, Waltham, MA, USA). Then ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK 293T and Flp-In HEK293 T-REx were maintained in DMEM-GlutaMAX at 4.5 g*ml-1 D-glucose (Gibco) supplied with sodium-pyruvate ...
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bioRxiv - Cancer Biology 2021Quote: ... we measured the luminance intensity of tumors by intraperitoneal injection of 50 mg/mouse D-luciferin (Thermo Fisher Scientific) into anesthetized mice ...
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bioRxiv - Cancer Biology 2020Quote: ... U2OS and HEK-239T cells were grown in DMEM containing 2.5 mM L-glutamine and 4.5 g l-1 D-glucose (Life Technologies). H1299 cells were grown in RPMI 1640 medium containing 2.5 mM L-glutamine.
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bioRxiv - Cancer Biology 2021Quote: A Gateway entry clone was generated using the Gateway pENTR/D-TOPO linear vector cloning kit (Thermo Fisher Scientific) to insert the ERα-GFP expression cassette into the Gateway donor vector ...
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bioRxiv - Immunology 2021Quote: ... CCR10 (248918) from R&D, CD11a (M17/4) from eBioscience, FcεRI (MAR-1), c-Kit (CD117, 2B8) from Invitrogen and Mouse BD Fc Block™ ...
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bioRxiv - Immunology 2021Quote: ... and biotinylated anti-Ym1/2 (polyclonal, R and D Systems Cat# BAF2446, RRID:AB_2260451) followed by Zenon anti–rabbit reagent (Thermo Fisher Scientific Cat# Z25302 ...
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bioRxiv - Immunology 2020Quote: 1×105 A549-ACE2 cells were reverse transfected using 20pmol of Non-targeting siRNA(D-001206-13-20) or IFITM2 siRNA (M-020103-02-0010) and 1μL of RNAi max (Invitrogen). Cells were incubated for 24h prior a second round of reverse transfection ...
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bioRxiv - Cell Biology 2021Quote: ... Another glass coverslip was sequentially coated with 0.1 mg mL−1 poly-D-lysine and 1:1000 ddH2O diluted red fluorescent beads (F8801, Invitrogen) for 30 min ...
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bioRxiv - Bioengineering 2022Quote: ... Neurons were plated onto poly-D-lysine coated glass bottom culture dishes in Dulbecco’s Modified Eagle Medium (Thermofisher Scientific) with 10% fetal bovine serum (Thermofisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... 60 μL of organoid digest buffer (Dispase [Corning, 354235, undiluted, 50 U/mL], DNase I [GoldBio, D-301, final concentration 0.25mg/mL], Collagenase Type I [Gibco, 17100017 ...
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bioRxiv - Immunology 2022Quote: ... BCRJ 0234) cell lines were maintained at 37ºC and 5% CO2 in DMEM (1 g/L D-glucose, GIBCO) supplemented with 20% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2022Quote: Cell culture derived HBV (genotype D) was prepared from supernatant of HepAD38 cells11 cultured in Advanced DMEM/F12 (ThermoFisher) supplemented with 10% FCS and 400μg/mL G-418 for ten days ...
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bioRxiv - Microbiology 2022Quote: A549-ACE2 cells were reverse transfected using 20pmol of Non-targeting siRNA (D-001206-13-20) or IFITM2 siRNA (M-020103-02-0010) with 1μL of RNAi max (Invitrogen). Cells were incubated for 24h prior to a second round of reverse transfection ...
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bioRxiv - Microbiology 2022Quote: The purified PCR products were cloned by mixing with the champion pET100/D-TOPO expression vector and ligated according to the manufacturer’s protocol (Invitrogen). The ligated vector containing the gene of interest (recombinant plasmid ...
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bioRxiv - Developmental Biology 2022Quote: ... The mCherry-SEpHluorin fragment was first amplified and then cloned into the Gateway entry vector pENTR/D-TOPO (Thermofisher) to build pENTR/D-mCherry-SEpHluorin ...
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bioRxiv - Neuroscience 2022Quote: ... cell membranes were loaded with the voltage-sensitive dye D-2-ANEPEQ (JPW1114, 0.2 mg/mL, Thermo Fisher Scientific) for 30 minutes using a first patch clamp recording and then re-patched a second time with dye free solution ...
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bioRxiv - Plant Biology 2022Quote: ... and SlJAZ9 (Solyc08g036640.2) genes were amplified by PCR (without stop codon) and cloned into pENTR/D-TOPO vector (Invitrogen) using the primers listed in Supplemental Table 2 generating TOPO-AtJAZ8 and TOPO-SlJAZ9 ...
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bioRxiv - Plant Biology 2024Quote: ... 8 and 6 hours were then transferred to MS medium containing zeocin with Actinomycin D (Thermo Fisher, J60148.LB0) or zeocin with DMSO (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were then treated with human serum containing phospholipase D (Australian Red Cross Lifeblood) or 0.25% (w/v) Albumax II (Gibco) and incubated for 3 hours at 37 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... the RNA and gDNA were physically separated using oligo(d)T-conjugated magnetic bead mRNA capture (MyOne C1, Invitrogen) and transfer of the gDNA containing lysate to a separate PCR plate.
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bioRxiv - Developmental Biology 2024Quote: ... the cells were transitioned to CNCC early maintenance media: 1:1 Neurobasal medium/D-MEM F-12 medium (Invitrogen), 1X penicillin-streptomycin solution ...
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bioRxiv - Cancer Biology 2024Quote: 24 hours before transfection 2.5 x 106 HEK293T cells were seeded 100 mm tissue culture-treated plates coated with 0.1 mg/L poly-D-lysine (Gibco). Cells were triple-transfected with 12.5 μg of the plasmid of interest (UCOE-SFFV-ZIM3-KRAB-dCas9-P2A-BFP plasmid or pDECKO_mCherry plasmid containing sgRNA) ...
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bioRxiv - Cell Biology 2024Quote: ... or si-ENG (Dharmacon #L-011026-00) or non-targeting scrambled control (Dharmacon #D-001810-10 or Ambion #4390843) constructs ...
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bioRxiv - Cell Biology 2024Quote: ... Glucose output of hepatocytes was also evaluated after overnight incubation in DMEM medium with no D-glucose (Invitrogen, USA) 37 ...
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bioRxiv - Cell Biology 2024Quote: ... R&D systems) and Corning® Matrigel® Growth Factor Reduced (GFR) Basement Membrane Matrix (Matrigel) (Fisher Scientific, #356231) at a 30/70 ratio ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were cultured in Dulbecco’s Modified Eagle Medium with 1 g/L D-glucose (low-glucose DMEM; Invitrogen, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2024Quote: A total of 5 × 103 Hek293T and HeLa cells were put into individual wells containing Dulbecco’s Modified Eagle Medium (D-MEM) [containing 10% Fetal Bovine Serum (FBS) and 1% Antibiotic-Antimycotic solution (Gibco)] ...