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Citations for Thermo Fisher :
5501 - 5550 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... The following secondary antibodies and dilutions were used: goat anti-mouse IgG(H+L) (Alexa Fluor 594, ThermoFisher, A-11005, 1:1000), and goat anti-rabbit IgG(H+L ...
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bioRxiv - Evolutionary Biology 2024Quote: ... a single colony was selected and grown anaerobically at 37 °C for 24-48 h in liquid tryptone-yeast extract-glucose (TYG) medium (10 g of Bacto Tryptone (Gibco, Cat. #211705), 5 g of yeast extract ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was isolated by the Trizol method and transcribed into cDNA using the RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Fisher Scientific). Real-time qPCR was performed using the Luna Universal Qpcr Master Mix (New England Biolabs) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed in PBS-T and incubated for 2 h (room temperature) with donkey anti-rabbit IgG (Alexa Flor™ 488; Invitrogen, A21206), donkey anti-goat IgG (Alexa Flor™ 568 ...
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bioRxiv - Plant Biology 2023Quote: ... First-strand cDNA was synthesized from 3 μg total RNA using a Maxima H Minus First Strand cDNA Synthesis Kit with dsDNase (Thermo Scientific, K1682) and an oligo(dT ...
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bioRxiv - Neuroscience 2023Quote: ... then washed with PBST and incubated with goat anti-mouse IgG (H+L) Alexa Fluor 594 (1:500 dilution by the blocking solution, A-11032, Thermo Fisher Scientific) at room temperature for at least 2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed with 0.1% Tween in PBS and incubated with donkey anti-rabbit IgG H&L Alexa Fluor™ 568 secondary antibody (1:1000, Invitrogen, A10042) and 1 μM Hoechst prior to imaging ...
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bioRxiv - Synthetic Biology 2024Quote: ... were washed three times in cold flow buffer and then the secondary antibody (Goat anti-Human IgG H+L Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488, ThermoFisher Scientific) at a dilution of 1:500 was added ...
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bioRxiv - Physiology 2023Quote: ... Samples were embedded in paraffin and 5 μm sections were cut and stained with hematoxylin and eosin (H&E) All images were captured using the EVOS FLoid imaging system microscope (Thermo Fisher Scientific). Adipocyte size was determined using Image J (https://imagej.nih.gov/ij/).
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bioRxiv - Cell Biology 2023Quote: ... and 24-48 h later incubated for 10 minutes in culture media (37 °C, 5% CO2) with MitoTracker Deep Red (200 nM, Thermo Fisher M22426). Imaging was performed at room temperature in mKRB using ZeissLattice Lightsheet 7 ...
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bioRxiv - Synthetic Biology 2024Quote: ... the cells were stained with Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody Alexa Fluor™ 488 (Invitrogen, A-11008) at dilution 1:500 for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed in PBS and incubated for 2 h at room temperature with a goat anti-chicken Alexa 488 secondary antibody (#A11039, 1/500, Invitrogen, MA, US) and goat anti-mouse Alexa 555 (#A21422 ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated for 60 minutes at RT in donkey anti-mouse IgG (H+L) (Alexa Fluor, 647, 2ug/mL) (Invitrogen A-11032). Coverslips were washed ...
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bioRxiv - Biophysics 2023Quote: ... GPMVs were subsequently labelled with a 1:500 dilution of Alexa Fluor 488 Donkey anti-Rabbit IgG (H+L) (Invitrogen, catalog # A21206) and gently agitated in the dark at RT for a minimum of 2-3 h ...
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bioRxiv - Bioengineering 2023Quote: ... the secondary antibody conjugated to Alexa Fluor® 647 goat anti-rabbit IgG (H+L) (Invitrogen, cat. No. A-21245, 1:500) was then treated for 1 h to visualize the antigen ...
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bioRxiv - Molecular Biology 2023Quote: ... RBM17- and SAP30BP-depleted HeLa cells were transfected at 48 h post-transfection with 0.5 µg of mini-gene plasmid (Supplementary Table S2) using lipofectamine 2000 reagent (Invitrogen–Thermo Fisher Scientific). These cells were incubated for 24 h prior to the extraction of RNAs described previously6 ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were washed with PBS to remove unbound antibody and incubated for 2 h with Alexa Fluor-conjugated secondary antibodies (Invitrogen; 1:500), 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were then washed three times in TBS-T and incubated for 1 h at RT with goat anti-rabbit or goat anti-mouse HRP conjugated secondary antibodies (31460 and 31430, respectively, Thermo Fisher Scientific) diluted 1:10000 in TBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... a series of every 6th section was immersed in 1% skim milk for 1 h at room temperature and incubated overnight at 4°C with rabbit anti-GFP monoclonal antibody (1:500, G10362, Thermo Fisher Scientific) i n PBS containing 0.1% Triton X-100 and 1% normal goat serum for 2 days at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... The membranes were then incubated with second antibodies including Alexa Fluor™ Plus 680 Goat anti-Rabbit IgG (H+L) (Invitrogen, RRID:AB_2633283) or Alexa Fluor™ Plus 800 Goat anti-Mouse IgG (H+L ...
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bioRxiv - Immunology 2023Quote: ... The plates were washed and incubated with 100 μL of a 1:10,000 diluted goat anti-mouse IgG (H+L) cross-adsorbed HRP conjugate antibody (Cat. # A16078; Invitrogen, Waltham, Massachusetts, USA) for 5 minutes on an orbital shaker (300 rpm ...
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bioRxiv - Microbiology 2023Quote: ... First-strand cDNA synthesis was performed on 1 µg of total RNA with the RevertAid H Minus Moloney murine leukemia virus (M-MuLV) reverse transcriptase (Thermo Fisher Scientific) using random primers p(dN)6 (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were reconstituted in 10 µl of H2O and reacted for 1 h at room temperature with TMT16pro labelling reagent (Thermo Scientific, #A44522). To this end ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed 3 times with PBS and a mixture of goat anti-mouse IgG(H+L) cross-absorbed secondary antibody conjugated to Alexa FluorTM 488 (Invitrogen #A-11001) and goat anti-rabbit IgG(H+L ...
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bioRxiv - Neuroscience 2023Quote: ... slices were washed and incubated 3 h at RT in secondary antibodies donkey-α-rabbit DyLight 650 (1:500, SA5-10169, Thermo Fisher) and donkey-α-mouse Alexa 568 (1:500 ...
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bioRxiv - Plant Biology 2023Quote: ... L−1 of 3’-biotin-labeled primer probe purified by high-pressure liquid chromatography (HPLC) for 4-12 h at 42°C in Hybaid Shake ‘n’ Stack (Thermo Fisher Scientific) with gentle rotation ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 h at 4°C before the addition of 25 μL of Pierce Protein A/G Magnetic Beads (Thermo Fisher Scientific) for an additional 2 h under constant rotation ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then rinsed five times in PBST and incubated for 1 h at RT with the secondary donkey anti-mouse Alexa488 (Life Technologies, Switzerland) used at a dilution of 1/800 in PBST ...
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bioRxiv - Plant Biology 2024Quote: ... and transferred to a 0.1 µm nitrocellulose membrane (Amersham Biosciences Protran) by semi-dry electroblotting for 1 h under constant current (60 mA) (Thermo Fisher Scientific) in filter paper soaked in transfer buffer (assembly order from the cathode to the anode ...
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bioRxiv - Molecular Biology 2023Quote: ... lysates were incubated for overnight at 4°C with 2 μg of antibodies recognizing MTR4 or IgG control and then incubated for 1 h at 4°C with Dynabeads™ Protein A (ThermoFisher Scientific). After incubation ...
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bioRxiv - Cancer Biology 2023Quote: ... dried peptides were resuspended in LC buffer and subjected to DDA-MS on a QExactive H-FX mass spectrometer (Thermo Fisher Scientific) equipped with an Easy-nLC 1200 (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2022Quote: ... followed by incubation with 488- or 594-conjugated secondary antibodies for 1 h prior to counterstaining and mounting with Prolong Diamond Antifade Mountant with DAPI (Thermo Fisher Scientific). Slides were imaged with a DeltaVision Elite deconvolution microscope ...
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bioRxiv - Microbiology 2022Quote: ... RNA samples were reverse transcribed with first strand synthesis primed with RT-primer 1 to 3 with a Maxima H Minus First Strand cDNA synthesis kit (Thermo Scientific, USA) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 150 embryos at 1 h after thawing were placed into a chamber with 40 µL of serum-free medium (Opti-MEM, Thermo Fisher Scientific) containing 100 ng/µL Cas9 protein and 200 ng/µL gRNA ...
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bioRxiv - Cell Biology 2022Quote: ... media was collected at the end of 48 h of lipid loading and albumin concentration was measured with a commercially available human albumin ELISA kit (Invitrogen, Waltham, MA).
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bioRxiv - Bioengineering 2022Quote: ... Next immunolabeling was performed over a duration of 24 h using donkey-anti mouse Alexa Fluor 555/647 (Invitrogen, dilution 1:500), donkey-anti rabbit Alexa Fluor 555/647 (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... 1% BSA in PBS), rinsed as above and incubated (1 h, RT) with goat anti-mouse Ig Alexa Fluor 488 (Thermofisher, RRID: AB_2534062), diluted 1:1000 in blocking solution ...
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bioRxiv - Cancer Biology 2022Quote: ... was used to extract total RNA and converted to cDNA using RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Scientific™). qRT-PCR analysis was done using FastStart Universal SYBR Green Master (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... PCR reaction mixture was loaded on 1% agarose gel (140 V for 2 h) and post-stained with SYBR gold (Thermo Fisher Scientific). The product bend was cut and DNA was extracted from the gel by Monarch DNA Gel extraction Kit (NEB) ...
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bioRxiv - Cell Biology 2022Quote: ... isolation was performed on HT1080-derived EV preparations as previously reported.14,26 Cell culture media was collected from cultures maintained at 80% confluent cells for 48 h in Opti-MEM (31985070, Thermo Fisher Scientific). The conditioned media were centrifuged at 300 × g for 5 min and 2,000 × g for 20 min to sediment live cells and cellular debris ...
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bioRxiv - Cell Biology 2022Quote: ... at a ratio of 1:50 (w:w) for 16 h at 37 °C and iTRAQ reagents kit (Applied Biosystems, Framingham, MA, USA) were used to label 100 micrograms of digested proteins according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2022Quote: ... The images of each well at 0 and 12 h post scratching was captured by EVOSTM M5000 Imaging system (Thermo Fisher Scientific) and the area of cell migration measured using Image J (NIH).
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bioRxiv - Cell Biology 2022Quote: ... Plates were washed and incubated 1 h with 1 μg/ml secondary Alexa Fluor 488-conjugated and Alexa Fluor 555-conjugated antibody (Thermo Fisher Scientific), Hoechst 33342 (1 μg/ml ...
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bioRxiv - Immunology 2022Quote: An Acquity Classic UPLC (Waters, UK) was coupled to an Orbitrap Fusion Lumos with the H-ESI source (Thermo Scientific, CA, USA). The injection quantities were 1 ug HI ...
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bioRxiv - Genetics 2022Quote: ... followed by a 1 h incubation with an Alexa-555 labeled Goat anti-rabbit antibody (Invitrogen A-21428, 0.8 μg ml−1). After three washes ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was then washed three times with PBST for 10 min before being incubated for 1 h with anti-rabbit horseradish peroxidase (HRP)-conjugated secondary antibody (Invitrogen, cat# 656120) diluted 1:2500 in PBST ...
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bioRxiv - Microbiology 2022Quote: ... They were then washed with PBS three times and incubated with secondary antibody: either Alexa Fluor 488 Goat Anti-Rabbit IgG (H+L) antibody (Thermofisher; A-11008) or Alexa Fluor 647 Donkey anti-Rabbit IgG (H+L ...
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bioRxiv - Microbiology 2022Quote: ... The cells were washed with PBS three times and then incubated for 2 h with 1:1000 goat anti-mouse Alexa 488 (Catalogue no. A11029, Invitrogen, CA, USA) and 1:1000 goat anti- rabbit Alexa 59 (Catalogue no ...
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bioRxiv - Microbiology 2022Quote: ... Cells were again centrifuged and washed with 1ml of 1x PBS before incubation with 1/500 diluted Goat anti-rabbit IgG (H+L) Alexa 555 (Thermo Fisher Scientific) in 100μl 1x PBS at 37°C for 1h ...
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bioRxiv - Neuroscience 2023Quote: ... slides were washed in PBS three times and incubated in goat anti-chicken (H&L) Alexa Fluor 488 (A11039; Invitrogen, Thermo Fisher Scientific) goat anti-mouse IgG1 Alexa Fluor 555 (A21127 ...