Labshake search
Citations for Thermo Fisher :
5201 - 5250 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... After sorting cells were resuspended in organoid medium (ENR) (DMEM/F-12, #11320033, Gibco; 1X PenStrep, #15140122, Gibco; 1X B27 supplement ...
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bioRxiv - Cell Biology 2024Quote: ... The combined cell mixtures were resuspended in minimal organoid medium (DMEM/F-12, Gibco, #11320074; 1X PenStrep, Gibco, #15140122 ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were seeded in 12-well plates (2.5 × 105 cells/well) coated with poly-D-lysine (Gibco, A3890401). Hormone treatments were done upon reaching 70-80% confluency ...
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bioRxiv - Cancer Biology 2024Quote: ... SNU-499 and Hep3B cells were seeded in multi-well 12 plates (Corning-Falcon, ThermoFisher Scientific, Stockholm, Sweden). Three days later ...
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bioRxiv - Microbiology 2024Quote: ... Samples of the elution fractions were analyzed by SDS-PAGE on a 4-12% Bis-Tris gel (Invitrogen) to check the presence and purity of the protein ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were cultured in propagation medium containing: DMEM/F-12 medium (Life technologies cat no 31331-028) with B27 supplement without vitamin A (Life Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... The next day cells were lipofected with a 600 µL formulation containing 12 µL Lipofectamine™ 2000 (Invitrogen), 2µg pCig2 Cre T2A H2B-mCherry ...
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bioRxiv - Molecular Biology 2024Quote: ... 5-30 µg of lysates were resolved on pre-cast 10% or 12% Bis-Tris NuPAGE gels (Invitrogen), transferred to PVDF membranes (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... NPC were plated on poly- L-ornithine/laminin-coated flasks at 50’000 cells/cm2 in2µg/ml laminin-supplemented neural expansion medium (DMEM/F-12 + Glutamax, Gibco® ...
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bioRxiv - Genomics 2024Quote: ... and then samples were minced and incubated while stirring for 1hr at 37°C in Dulbecco’s modified Eagle’s medium/F-12 (Invitrogen) media containing collagenase (Invitrogen ...
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bioRxiv - Genetics 2024Quote: ... The rest of the embryo was placed into a 12-well plate filled with 2 ml ice-cold 0.25 % Trypsin/PBS (diluted from 2.5 % Trypsin, Gibco). The embryos were chopped into small pieces and incubated overnight at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... Coomassie G-250 additive was added to samples and loaded onto NativePAGE 3-12% Bis-Tris gels (Invitrogen). Samples were first run in dark blue cathode buffer (inner chamber ...
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bioRxiv - Genomics 2024Quote: ... Equal protein amounts from each fraction were run on 4-12% bis-tris plus acrylamide gels (Invitrogen, NW04122) and then immunoblotted ...
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bioRxiv - Immunology 2024Quote: ... Coomassie G-250 additive was added to samples and loaded onto NativePAGE 3-12% Bis-Tris gels (Invitrogen). Samples were first run in dark blue cathode buffer (inner chamber ...
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bioRxiv - Biochemistry 2024Quote: ... 10 µL of this mix was added into wells of a 12-well 6% DNA retardation gel (Invitrogen) that was pre-running for at least 30 minutes in 0.5X TBE at 150V ...
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bioRxiv - Immunology 2023Quote: ... human embryonic kidney HEK293 and the African green monkey kidney-derived Vero cells were cultured in Dulbecco’s modified Eagle’s medium F-12 (DMEM F12) (Gibco) supplemented with 5% foetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... and equivalent concentrations (10-16ug) loaded per sample on precast NuPAGE 4-12% Bis-Tris gradient gels (Invitrogen). Separated proteins were transferred to nitrocellulose and probed overnight with primary antibodies under agitation ...
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bioRxiv - Microbiology 2024Quote: ... The extracted proteins were stacked in the top of an SDS-PAGE gel (4-12% NuPAGE, Life Technologies), stained with Coomassie blue R-250 (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... at 95⁰C for 3 min and loaded onto a SDS-polyacrylamide gel (4-12%, Invitrogen NuPAGE, #NP0336BOX). After size separation ...
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bioRxiv - Cell Biology 2023Quote: Human RPE-1 cells were cultured in Dulbecco’s Modified Eagle Medium: Nutrient Mixture F- 12 (DMEM/F12) (GIBCO), supplemented with 10% (v/v ...
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bioRxiv - Genetics 2023Quote: ... The reaction mixtures were run at 4°C in native 12% Tris-Glycine PAGE gel (Invitrogen, Waltham, MA) at 160V for 40min ...
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bioRxiv - Cancer Biology 2024Quote: ... Psen1-/- Psen2-/- mouse embryonic fibroblasts (MEFs) and HEK293T/17 cells were cultured in DMEM/F-12 (Fisher scientific) with 10% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... 5% v/v 2-mercaptoethanol) and fractionated by 4–12% Bis-Tris gel (Thermo Fisher Scientific Cat#NP0335BOX) using MES running buffer (Thermo Fisher Scientific Cat#NP000202) ...
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bioRxiv - Cell Biology 2024Quote: ... The rest of the embryo was placed into a 12-well plate filled with 2 ml ice-cold 0.25 % Trypsin/PBS (diluted from 2.5 % Trypsin, Gibco). The embryos were chopped into small pieces and incubated overnight at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... 12 µm transverse sections of the retina were cut and mounted onto SuperFrost Plus™ slides (Fisher Scientific). Sections from control and treated eyes from the same individual were placed consecutively on each slide to ensure equal exposures to reagents ...
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bioRxiv - Neuroscience 2023Quote: SH-SY5Y cell lysates expressing ATXN3-84Q were separated in 4-12 % NuPAGE Bis-Tris polyacrylamide gels (Invitrogen) in duplicate gels ...
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bioRxiv - Microbiology 2023Quote: ... fresh Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12) (Gibco®, Life Technologies, Carlsbad, CA, USA), supplemented with 2% heat-inactivated fetal bovine serum was added ...
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bioRxiv - Microbiology 2023Quote: ... fresh Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12) (Gibco®, Life Technologies, Carlsbad, CA, USA), supplemented with 2% heat-inactivated fetal bovine serum was added ...
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bioRxiv - Microbiology 2023Quote: ... the supernatants were electrophoresed on NativePAGE Novex 3–12% Bis-Tris protein gels as per manufacturer’s instructions (Invitrogen). Briefly ...
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bioRxiv - Biochemistry 2023Quote: The protein (wildtype or Y20A mutant) was pegylated with MS(PEG)12 methyl-PEG-NHS-ester (Thermo Fisher) at 1:30 or 1:60 molar ratio on ice for 2 hours and then proceed to grid freezing ...
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bioRxiv - Biophysics 2023Quote: ... The purification efficiency was monitored by electrophoresis using NuPAGE® Novex® 4-12% Bis-Tris Gels (Invitrogen™ by LifeTechnologies™ ...
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bioRxiv - Biochemistry 2023Quote: ... the medium was replaced by 12 ml of OptiMEM medium (Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA). Twenty-6 h after transfection ...
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bioRxiv - Cell Biology 2023Quote: Mitochondrial isolates (15 μg) were separated by electrophoresis using 3-12% NativePAGE gels (Life Technologies Australia, Mulgrave, Australia) to assess supercomplex formation using a 4 g/g digitonin/protein ratio ...
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bioRxiv - Genomics 2023Quote: ... then heated at 100°C for 5 minutes and loaded onto a 4-12% polyacrylamide gel (ThermoFisher NP0322). Transfer onto a PVDF membrane was performed in 1X NuPage Transfer Buffer (ThermoFisher NP00061 ...
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bioRxiv - Developmental Biology 2023Quote: ... 20% sucrose in 0.1M PB and sectioned (12 or 14 µm) at the cryostat (CryoStar Nx70, Thermo Scientific). Cryosections were stored at -20°C.
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bioRxiv - Developmental Biology 2023Quote: ... and incubated at 37L for 15 min with 1x TripLE Express Enzyme (Thermo Fisher Scientific, #12-605-010). Organoids were dissociated by pipetting up and down with a 1000 µl pipette followed by 200 µl pipette until a single cell resuspension was obtained ...
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bioRxiv - Developmental Biology 2024Quote: ... or immediately transitioned to CNCC late maintenance media: 1:1 Neurobasal medium/D-MEM F-12 medium (Invitrogen), 1X penicillin-streptomycin solution ...
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bioRxiv - Cell Biology 2024Quote: ... Protein samples were heat denatured and separated on 4–12% Bolt Bis-Tris gels (Thermo Fisher Scientific: NW04125BOX). The protein was then transferred onto a 0.2μM nitrocellulose membrane (VWR ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 μl of the protein samples were loaded onto NuPage 4–12% Bis–Tris polyacrylamide gels (Invitrogen, NP0321) and separated via electrophoresis ...
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bioRxiv - Bioengineering 2023Quote: ... a ‘shim’ photomask reflecting the geometry of a standard 1’’ x 3’’ microscope slide (12-559-A3, ThermoFisher) was printed at 20,000 d.p.i (CAD/Art Services ...
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bioRxiv - Neuroscience 2022Quote: ... and electrophoresis was performed in NuPAGE 4 to 12% gradient Bis-Tris polyacrylamide protein gels (Thermo Fisher Scientific). Proteins were transferred to Immun-Blot PVDF membranes (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were loaded onto a NuPAGE 4-12% Bis-Tris pre-cast SDS gradient gel (Thermo Fisher Scientific), transferred to a nitrocellulose membrane (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... Complete hNSC serum free media (100 ml) comprised 97 mL Knockout DMEM/F-12 (Gibco®, 12660-012), 1 mL GlutaMAX™ (Gibco® ...
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bioRxiv - Cell Biology 2022Quote: ... the cell lysates were run onto NuPAGE®4–12% Bis-Tris Gels (Novex Life Technologies, Waltham, MA) and transferred onto PVDF membrane using the Bio-Rad transfer system ...
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bioRxiv - Neuroscience 2022Quote: 12-well cell culture inserts with 0.4 µm pore size polycarbonate membranes (catalog number 140652, Thermo Fisher Scientific), deployed for the transport assay ...
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bioRxiv - Biochemistry 2022Quote: ... and then analysed on Novex WedgeWell 4-12 % Tris-Glycine Gels or 3-8 % Tris-Acetate gels (Invitrogen). Tris-Glycine gels were run at 180 V for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting 12 fractions were then analyzed by LC-MS/MS on a Q Exactive Plus (Thermo Scientific) as previously described50.
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bioRxiv - Cell Biology 2022Quote: ... The heated samples were briefly centrifuged at high speed before being resolved on 4-12% NuPAGE gel (Invitrogen). Proteins were transferred onto a PVDF membrane filter paper sandwich (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: Samples were either run on 4-12% NuPAGE or Bolt gels with the NuPAGE Bis-Tris system (Invitrogen) in MOPS SDS buffer or on 8% gels with the BIO-RAD system with SDS-running buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Protein gels were run according to standard procedure on a 4-12% Bolt Bis-Tris Plus gel (Invitrogen), blocked with Intercept Blocking Buffer (LI-COR) ...