Labshake search
Citations for Thermo Fisher :
5451 - 5500 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... Muscle tissue was obtained from lower limbs and incubated for 15 min at 37℃ in a collagenase type-2 (1.5 mg/mL; Gibco, No.17101-015), dissolved in PBS 1X and filtered with 0.22 μm filters ...
-
bioRxiv - Cell Biology 2024Quote: Mitochondria were isolated from wild-type and TMX2 KO cells using the Mitochondria Isolation Kit for Cultured Cells (89874, Thermo Fisher Scientific) according to the manufacturer’s protocol (Option A) ...
-
bioRxiv - Neuroscience 2024Quote: ... Mobile phase A was ultrapure water of ASTM I type (resistance 18.2 MΩ.cm at 25°C) prepared by Barnstead Smart2Pure 3 UV/UF apparatus (Thermo Fisher Scientific, Bremen, Germany) with 0.1% (v/v ...
-
bioRxiv - Molecular Biology 2024Quote: ... cells were transfected with 100ng HA-HALO-MC3R and 100 ng pcDNA or FLAG-MRAP2 (wild-type of mutant) and cells fixed 48-hours later in 4% PFA (Fisher Scientific UK) in PBS ...
-
bioRxiv - Neuroscience 2024Quote: ... and digested in 200 μl of collagenase buffer with 200 units of collagenase type IV (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) at 37°C for ∼3.5 hours ...
-
bioRxiv - Biochemistry 2024Quote: ... S1 fraction was washed from the filter using fresh full media supplemented with 100 U/mL collagenase type IV (Life Technologies, 17104019), and 15 mM HEPES (Gibco ...
-
bioRxiv - Cancer Biology 2024Quote: ... 300 cells in 20 μl of medium were seeded in 384 well plates by a microplate dispenser (Type 836, Thermo Scientific Combi). HPAC was seeded at 500 cells/well ...
-
bioRxiv - Bioengineering 2024Quote: ... Tissue was then placed into a 15mL conical tube containing 9mL 0.1% (w/v) filter-sterilized Collagenase Type II (Thermo Fisher Cat#17101015) in 1X PBS and 1mL filter-sterilized 2.5 units/mL dispase II (Thermo Fisher Cat#17105041 ...
-
bioRxiv - Cell Biology 2024Quote: ... cut into half and measured as two technical replicates) were digested with 0.2% collagenase type II and 0.05% hyaluronidase in reduced serum OptiMEM-I (Gibco-BRL, Grand Island, NY) media supplemented with 50 µg/mL gentamycin for 3h at 37°C with frequent agitation on a tube rotator (model ...
-
bioRxiv - Molecular Biology 2024Quote: The wild-type FASTKD5 cDNA in pDONR221 17 was cloned into Gateway modified pBABE-3xFLAG-Puro plasmid 18 using Gateway technology (Thermo Fisher Scientific). The single point mutations and FASTKD5 deletion mutants were engineered using QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent ...
-
bioRxiv - Molecular Biology 2024Quote: ... T6B wild type (WT) and T6B inactive mutant expressing vectors 41 were transfected in DIvA cells with Lipofectamine 2000 (Invitrogen; #11668-027) for 24 hours before RNA extraction ...
-
bioRxiv - Molecular Biology 2024Quote: Wild-type K562 cells (ATCC, CCL-243) were grown in Roswell Park Memorial Institute (RPMI) 1640 Medium (Thermo Fisher, Cat#11875-119,) supplemented with 10% (v/v ...
-
bioRxiv - Neuroscience 2024Quote: ... a type I afferent fiber bundle of one RTN4RL2-/- dataset was volume traced and 3D rendered using Amira software (Thermo Scientific, US).
-
bioRxiv - Physiology 2024Quote: ... TK-LUC reporter and wild-type or mPPARγ C313A were transfected into cells was transfected via TurboFect™ transfection reagent (cat. no. R0532, Thermo Fisher). After additional 24 and 48 hrs ...
-
bioRxiv - Immunology 2024Quote: ... Lung was cut into pieces and digested for 1h at 37°C with 150 U/ml Collagenase type I (Gibco, 17100-017). Pieces were fully disrupted through 100µm strainers and pellet resuspended in 44% Percoll (Citiva/Fisher) ...
-
bioRxiv - Cancer Biology 2024Quote: ... LuCaP145.2 tumors were minced into small pieces (∼1 mm3) and digested in 5 mg/ml collagenase type II (Gibco, Cat#17101-015) with 10 μM Y-27632 dihydrochloride (Tocris ...
-
bioRxiv - Cell Biology 2024Quote: ... the cells were transfected with the pCMV6-AC-GFP vector and gigaxonin wild-type constructs plus Lipofectamine 3000 and p3000 reagents that were utilized in accordance with product instructions (Thermo Fisher Scientific). A Lipofectamine/p3000 only condition was utilized as an untransfected control ...
-
bioRxiv - Biochemistry 2024Quote: All buffers and reagents were prepared with reagent-grade chemicals and Type I water purified with a Smart2Pure 6 UV/UF system (ThermoFisher, Waltham, MA). Solutions were sterilized through a 0.22 μm PES filter.
-
bioRxiv - Cell Biology 2024Quote: ... and adductors) were minced and sequentially incubated in 800 units/mL collagenase type II solution (catalog number: 17101-015, Life technologies, Gibco®) and subsequent incubation with 80 units/mL collagenase II and 2 units/mL dispase II (catalog number ...
-
bioRxiv - Bioengineering 2020Quote: ... (anti-Claudin-1, anti-Claudin-5, anti-Occludin, anti-ZO-1; Invitrogen), in a 1:100 dilution in 10% donkey serum in PBS with 0.1% Saponin and incubated overnight on the Brain-Chip at 4°C ...
-
bioRxiv - Genomics 2022Quote: ... + 1x Anti-Anti (Gibco)) ...
-
bioRxiv - Biophysics 2022Quote: ... and Anti-Anti (Gibco). P2 virus was added to cells ...
-
bioRxiv - Neuroscience 2021Quote: ... and Anti-Anti (ThermoFisher) and kept at 37°C in 5% CO2 ...
-
bioRxiv - Cancer Biology 2020Quote: ... 1X Anti-Anti (Gibco) and 10% FBS ...
-
bioRxiv - Cancer Biology 2020Quote: ... 1X Anti-Anti (Gibco) and 10% FBS ...
-
bioRxiv - Cancer Biology 2020Quote: ... 1X Anti-Anti (Gibco) and 10% FBS.
-
bioRxiv - Cancer Biology 2022Quote: ... + 1% Anti-Anti (Gibco)) ...
-
bioRxiv - Neuroscience 2022Quote: ... 1% Anti-Anti (Invitrogen); 0.2% β-mercaptoethanol (Invitrogen) ...
-
bioRxiv - Developmental Biology 2024Quote: ... 1% anti-anti (Gibco), and 5% PFHM-II (Gibco ...
-
bioRxiv - Cancer Biology 2023Quote: ... and anti-anti (Invitrogen, containing 100 μg/mL streptomycin ...
-
bioRxiv - Cancer Biology 2024Quote: ... 2% Anti-Anti (Gibco), 13 mM d-glucose (Sigma-Aldrich) ...
-
bioRxiv - Biophysics 2023Quote: ... and Anti-Anti (Gibco). P2 virus was added to cells ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... anti-anti (Thermo Fisher) in 5% CO2 at 37 °C until 80% confluency ...
-
bioRxiv - Biophysics 2024Quote: ... 0.5X Anti-Anti (Gibco), 20 mM Sodium Lactate (Sigma-Aldrich) ...
-
bioRxiv - Microbiology 2020Quote: ... Samples were heated 5 min at 65°C and loaded onto a Novex Wedgewell 12% Tris-glycine gel (Invitrogen). Each sample was loaded on two different wells and protein’s migration was then performed according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... 12 spheroids from each group were collected and digested using 2 mg/ml collagenase IV (Therma Fisher Scientific, MA) for one hour with stirring to generate a single cell suspension ...
-
bioRxiv - Cell Biology 2020Quote: ... SDS-PAGE separation was performed using 1.5 mm 4-12% Bis-Tris pre-cast polyacrylamide gels (Novex, ThermoFisher Scientific) and 1X MES SDS NuPAGE™ Running Buffer (ThermoFisher Scientific) ...
-
bioRxiv - Cell Biology 2020Quote: ... BN-PAGE was performed using NativePAGE 3-12% or 4-16% Bis-Tris gels and associated running buffer (Invitrogen). The cathode buffer was supplemented with 0.002% (w/v ...
-
bioRxiv - Cell Biology 2020Quote: HepG2 cells were cultured and maintained in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12; Life Technologies), supplemented with 10% (v/v ...
-
bioRxiv - Cell Biology 2020Quote: ... proteins were separated by SDS-PAGE on a 4-12% NuPAGE BisTris gradient gel (Thermo Fisher Scientific/Life Technologies) and stained using colloidal Coomassie Brilliant Blue ...
-
bioRxiv - Cell Biology 2020Quote: ... proteins were separated by SDS-PAGE on a 4-12% NuPAGE BisTris gradient gel (Thermo Fisher Scientific/Life Technologies) and stained using colloidal Coomassie Brilliant Blue ...
-
bioRxiv - Genetics 2021Quote: ... 10 μg of protein were loaded onto a 15-well NuPAGE™ 4-12% Bis-Tris Protein Gels (Invitrogen), run at 100 V for 2.5 hours ...
-
bioRxiv - Microbiology 2020Quote: ... then heated to 42°C for 15 min before loading to 12% Novex Tris-Glycine SDS-PAGE gels (ThermoFisher). The eluted protein was resolved by electrophoresis at 150 V for 60 min ...
-
bioRxiv - Immunology 2021Quote: ... 10ug of protein per sample was resolved on NuPage 4-12% Bis-Tris precast gels in MES buffer (Invitrogen), transferred to 0.45μm PVDF membrane then blocked with 5% BSA in TBS supplemented with 0.1% Tween-20 ...
-
bioRxiv - Developmental Biology 2020Quote: ... were isolated from the embryos and cultured using a Durapore Membrane Filter 0.65 μm (#DVPP02500) placed on a stainless-steel mesh for up to 7 days in Ham’s F-12/DMEM (Gibco) supplemented with Insulin-Transferrin-Sodium Selenite Supplement (Roche ...
-
bioRxiv - Developmental Biology 2021Quote: ... and the sections were transferred to glass slides (Superfrost Plus Microscope Slides, Fisher Scientific, cat. no. 12-550-15). For immunolabeling ...
-
bioRxiv - Cell Biology 2022Quote: ... 150 µg of protein extract from each sample were ran on NuPAGE 4%–12% Bis–Tris Protein Gels (Invitrogen) using MES running buffer (100 mM MES ...
-
bioRxiv - Molecular Biology 2020Quote: ... ∼50 µg of protein extracts was then resolved on precast NuPAGE Novex 4–12% Bis-Tris gels (Invitrogen, NP0321BOX). The proteins were transferred to a nylon membrane with the semidry transfer Pierce Power System (ThermoFisher Scientific ...
-
bioRxiv - Genomics 2021Quote: ... filtered through a 35 µm strainer and stained with 3x staining buffer (12 µM DAPI, 0.4 U/µl Ambion RNase Inhibitor ...
-
bioRxiv - Microbiology 2020Quote: Calu-3 human airway epithelial cells (ATCC® HTB55) were cultured in Dulbecco’s modified Eagle’s medium (DMEM)-Ham F-12 medium (1:1) (Gibco) supplemented with 10% fetal calf serum (FCS) ...