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Citations for Thermo Fisher :
5051 - 5100 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Proteins were separated using NuPAGE 4 to 12% Bis-Tris gels and transferred onto nitrocellulose membranes (Life Technologies). The blots were blocked with 5% nonfat dry milk at room temperature for 1 h and incubated overnight at 4°C with desired primary antibodies at concentration per the manufacturer’s protocol ...
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bioRxiv - Biophysics 2020Quote: ... We then used these three-cell flow chambers together with glass slides (Thermo Fisher Scientific, 12-550-123) and 170 μm thick coverslips (Zeiss ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were seeded with 2×105 density in a 1 mL medium in 12-well plates (ThermoFisher Scientific). After 24-hour incubation ...
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bioRxiv - Cancer Biology 2021Quote: ... then separated with SDS-PAGE (Nupage™ 4-12% Bis-Tris Protein Gels, 1.0 mm, Invitrogen™, NP0322BOX). Protein was transferred onto a nitrocellulose membrane (Thermo Scientific™ ...
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bioRxiv - Cell Biology 2021Quote: ... in DMEM-F2 medium (DMEM-F2, Dulbecco’s modified Eagle medium: nutrient mixture F-12 (Gibco, Grand Island, EUA) containing 10% of characterized fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... 1 µg of sample was loaded on 4–12% NuPAGE Novex Bis-Tris polyacrylamide gels (Thermo Scientific, NP0322BOX) and ran for 7 min at 80V to stack proteins in a single band ...
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bioRxiv - Cell Biology 2020Quote: ... was taken out from the mold and 12 μm coronal slices were sectioned on the cryostat (Thermo Scientific). Sections were collected on Superfrost Plus glass slides (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... A total of 20 μg protein was separated in the 4%-12% NuPAGE gel (NP0321BOX/NP0322BOX, Invitrogen, USA) and was transferred to 0.2 μm nitrocellulose membranes (Millipore ...
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bioRxiv - Cell Biology 2021Quote: Soluble proteins (10 to 50 μg) were resolved on NuPAGE 4-12% Bis-Tris Protein Gels (Life Technologies) or on SDS-PAGE gels (12 or 15% ...
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bioRxiv - Cell Biology 2020Quote: Eluted proteins were resolved on a 10-well NuPAGE 4-12% Bis-Tris protein gel (Thermo Fisher Scientific), stained with Coomassie (Imperial Protein Stain ...
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bioRxiv - Cancer Biology 2020Quote: ... BT-12 and BT-13[7] were cultured in Dulbecco’s modified Eagle’s medium with Nutrient Mixture F-12 (DMEM/F12, Gibco) supplemented with 2 mM L-glutamine ...
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bioRxiv - Genomics 2020Quote: ... Equal amounts of total protein were loaded on a 4-12% polyacrylamide Bis-Tris gel (Thermo Scientific #NW04120BOX) and separated by electrophoresis in MOPS buffer at 200 V in a Bolt gel tank (Thermo Scientific #A25977 ...
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bioRxiv - Cell Biology 2022Quote: ... resuspended in culture media (DMEM:F-12 media supplemented with 1x B27 and penicillin/streptomycin (P/S) (ThermoFisher Scientific)) and seeded in 48-well plates (3000-4000 cells/well ...
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bioRxiv - Cell Biology 2022Quote: 40 ug of RPE cell lysates were separated through SDS-PAGE on a 4–12% NuPAGE gel (Invitrogen) and transferred to an Immobilon-FL polyvinylidene fluoride membrane (IPFL00010 ...
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bioRxiv - Neuroscience 2022Quote: ... 40 μg of samples’ proteins were loaded on a Precast NuPage 4-12 % Bis-Tris gels (Thermofisher, USA) and transferred onto a nitrocellulose membrane (GE healthcare life science ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were then subjected to SDS-PAGE separation using NuPage 12% Bis-Tris mini protein gels (Thermo Fisher) according to the manufacturer’s instructions and gel sections comprising the de-glycosylated band of PrP were excised ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples containing 15 µg of protein were separated on 3–12% Bis-Tris Novex NativePAGE gel (Life Technologies). The relative levels of the assembled respiratory complexes I-V were assessed by western blot with following antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein samples (50 μg) were resolved by one-dimensional denaturing 12 % Novex Trisglycine gels (Invitrogen, Burlington, ON, Canada) and transferred onto polyvinylidene difluoride membranes (PVDF ...
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bioRxiv - Neuroscience 2022Quote: Cell lysates were resolved using SDS polyacrylamide gel electrophoresis (PAGE) on 4-12% Bis-Tris Plus gel (Invitrogen), transferred to PVDF membrane (Invitrogen) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... SUM159 cells were cultured in Dulbecco’s modified Eagle’s medium: Nutrient Mixture F-12 (DMEM/F12; Gibco, 11330-032) supplemented with 5% FBS ...
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bioRxiv - Microbiology 2022Quote: ... All lysates were separated by SDS-PAGE on pre-cast 4-12% Bis-Tris gels (Thermo Fisher Scientific) in the NuPAGE electrophoresis system ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of protein were loaded into Nu-Page Novex 4-12% Bis-Tris protein gels (Invitrogen, NP0322) and electrophoresis run in MOPS buffer (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... COS-7 cells were plated onto Nunc Lab-Tek II Chambered Coverglass (Thermo Fisher Scientific, 12-565-335) to achieve ~70% confluence before transfection.
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bioRxiv - Cancer Biology 2022Quote: ... PyMT and Met1 cells were maintained in Dulbecco’s Modified Eagle’s Medium: Nutrient Mixture F-12 (DMEM/F12; Gibco) supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... whole protein extracts from fungus or host cells were separated on NuPAGE 4%–12% Bis-Tris Gels (Invitrogen) and transferred to 0.2-µm pore size PVDF membranes (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... Bone chips were plated in 12-well and 6-well plates in MEM-A medium (GIBCO, 12561-056) supplemented with 10% FBS (Cytiva ...
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bioRxiv - Evolutionary Biology 2022Quote: ... cDNA libraries were amplified using 12-13 PCR cycles and quantified on a Qubit Fluorometer (Thermo Fisher Scientific). Average fragment size was determined on a Fragment Analyzer (Agilent) ...
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bioRxiv - Cell Biology 2022Quote: ... The following antibodies and dilutions were used: EpCam-PE (1:500, Thermo Fisher Scientific Cat# 12-5791-82); EpCam-APC (1:500 ...
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bioRxiv - Cancer Biology 2022Quote: ... 40 μg of total protein was separated in Bolt 4-12% Bis-Tris Plus Gels (Thermo Fisher Scientific) and transferred to Hybond P 0.45 PVDF membranes (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... Equal protein amounts were separated by SDS-PAGE (NuPage® Novex® 4–12% Bis-Tris gels, Invitrogen) and transferred to nitrocellulose membranes using an iBlot® device (iBlot®Gel Transfer Stacks ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal quantities of protein (10-20µg) were loaded on a 4-12% NuPAGE Bis-Tris gel (ThermoFisher #NP0335BOX) and run at 120-150V ...
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bioRxiv - Neuroscience 2020Quote: ... 30 μg of denatured protein was loaded onto a 4-12% Bis-Tris polyacrylamide gel (Invitrogen, Cat. #NP0321PK2). Five μL of Amersham ECL Rainbow Marker was included as a molecular weight standard (Millipore Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... Immunoprecipitated proteins were separated on a 4–12% Bis-Tris gel and stained with SafeStain (ThermoFisher Scientific, #LC6060). A broad band around 50 kDa was cut out from the gel ...
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bioRxiv - Microbiology 2021Quote: ... Samples were subsequently separated by SDS-Polyacrylamide gel electrophoresis using 1mm thick 4-12% Bis-Tris gel (Invitrogen), followed by staining with Instant Blue Coomassie stain (Expedeon Inc ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein purity was assessed by SDS-PAGE (NuPAGE gels; 12% bis-tris gels, and MOPS buffer both Invitrogen). Dynamic Light Scattering (Zetasizer nano ZS ...
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bioRxiv - Molecular Biology 2021Quote: ... and SH-SY5Y cells in Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 (DMEM/F12; 1:1 mixture; Gibco) each containing 10% heat-inactivated fetal calf serum (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... then loaded on a NuPAGE® Novex® 4-12% Bis-Tris Protein gel (Life Technologies, cat# NP0321). Protein was transferred onto a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... the supernatant was aspirated and the pellet was resuspended in rabbit fibroblast media (DMEM/F-12 (Life Technologies) supplemented with 10% fetal bovine serum ...
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bioRxiv - Cancer Biology 2021Quote: ... The lysates were then resolved by electrophoresis in Bolt 4 – 12% Bis-Tris Plus Gels (Thermo Fisher Scientific) followed by western blotting as described previously (25) ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were transfected with DNA constructs (1ug/well in 12-well plate) using Lipofectamine 2000 reagent (Invitrogen, 11668019) in Opti-MEM medium (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples and standards were loaded onto 4–12% Tris–glycine or 10% Bis-Tris acrylamide gels (NuPAGE, Invitrogen), then transferred onto 0.45 μm nitrocellulose membrane ...
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bioRxiv - Neuroscience 2021Quote: ... Approximately 10 µg of protein preparations were loaded onto precast NuPAGE 4-12% Bis-Tris gels (NP0335BOX, Invitrogen) for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Zoology 2021Quote: ... 71% of the samples were analyzed with QuantStudio™ 12 K Flex Real-Time PCR System (Thermo Fisher) using 384-well qPCR plates ...
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bioRxiv - Biochemistry 2021Quote: ... Equal amounts of protein (10 μg) were separated under reducing conditions on 4–12% Novex WedgeWell gels (Invitrogen) and transferred to PVDF membranes (Advansta) ...
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bioRxiv - Neuroscience 2020Quote: ... Lysates were run on Novex pre-cast mini gels (NuPAGE 4-12% Bis-Tris Gels, NP0322BOX, Thermo Scientific) in either 1 x MES or 1 x MOPS buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... 13μg of each protein sample was loaded onto NuPAGE 4-12% Bis-Tris Protein gels (Thermo Fisher Scientific), and samples were run in NuPAGE MOPS SDS Running Buffer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... treated coverslips and incubated at 37°C for 2-6 hours in Dulbecco’s modified Eagle’s medium (F-12/DMEM; Invitrogen) supplemented with 1% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... and samples were run on a NuPAGE 4-12% Bis-Tris Gel in MES SDS Running Buffer (Invitrogen). For FCG-Cas9 and FCG-Cas9 Nf1/p53 CRISPR samples ...
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bioRxiv - Cancer Biology 2021Quote: ... and samples were run on a NuPAGE 4-12% Bis-Tris Gel in MES SDS Running Buffer (Invitrogen). For wildtype mouse astrocyte and human GBM samples ...
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bioRxiv - Neuroscience 2020Quote: ... Ten micrometers-thick cryosections were collected on Fisherbrand Superfrost Plus Microscope Slides (Thermo Fisher Scientific, 12-550-15). The sections containing the optic nerve were analyzed for anatomical reference.