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Citations for Thermo Fisher :
5151 - 5200 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Dlk1+ enriched cells were seeded in Expansion media (DMEM/F-12, GlutaMAX™ supplement (ThermoFisher, Cat. No. 31331028), 10% FBS ...
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Myosin VI regulates ciliogenesis by promoting the turnover of the centrosomal/satellite protein OFD1bioRxiv - Cell Biology 2021Quote: hTERT-RPE1 cells (ATCC) were maintained in Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F12, Gibco), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Developmental Biology 2021Quote: ... according to manufacturer’s instructions and stained with Nur77 (12.14) antibody (Thermo Fisher Scientific, Cat. No.: 12-5965-82). All antibodies were from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... 5-10µg of protein per sample was run on a 4%–12% NuPAGE Bis-Tris Gel (ThermoFisher Scientific) and then transferred to PVDF membrane by liquid transfer using NuPAGE Transfer buffer (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated using NuPAGE 4-12% precast Bis-Tris protein gels in MOPS SDS running buffer (Invitrogen), transferred onto Immobilon-P PVDF membranes (Millipore) ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amount of protein (micro-BCA method; Pierce, IL) were loaded on 4-12% gradient gels (Invitrogen, California), electrophoresed and transferred to PVDF membrane ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Each sample (15 µg) was loaded and run in a NuPAGE 4-12% Bis-Tris gel (Invitrogen, NP0322BOX) on the Mini Gel Tank (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 μl of 100 μl of sample was loaded onto 4-12% Novex Tris-glycine gel (Invitrogen, XP04120BOX). Western blotting was done using an anti-FLAG antibody ...
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bioRxiv - Bioengineering 2023Quote: ... The two specimens (4 wk and 12 wk) were loaded into a Helios G4 UXE DualBeam (ThermoFisher Scientific) microscope with a xenon plasma source for serial sectioning ...
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bioRxiv - Developmental Biology 2023Quote: ... 20 μg/protein was loaded per well into prepared SDS-PAGE gels (Invitrogen NuPAGE 12% Bis-Tris gels). Proteins were transferred to PVDF membranes and immunoblotted for visualization with Immobilon Western chemiluminescent HRP substrate (Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... reporter viruses.17,18 Huh7+AT cells were cultured in Dulbecco’s modified Eagle essential minimal medium nutrient mixture with Ham’s F-12 medium (DMEMF12; Gibco) supplemented to a final concentration of 10% with heat-inactivated fetal bovine serum and 1% penicillin-streptomycin-L-glutamine (PSG ...
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bioRxiv - Microbiology 2023Quote: ... Samples were then separated using a 12% SDS-PAGE and visualized using the Pierce Silver Stain Kit (ThermoFisher). The PectA bait and ectB negative control were loaded next to each other in order of increasing NaCl concentrations ...
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bioRxiv - Microbiology 2023Quote: ... and loaded onto a 10-well Novex NuPAGE 4% to 12% BT SDS-PAGE gel (Invitrogen, Carlsbad, CA). Electrophoresis was performed in MOPS running buffer at 150 V ...
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bioRxiv - Neuroscience 2023Quote: ... 10 uL of each sample was resolved by electrophoresis using a 4-12% NuPAGE (Thermo Fisher Scientific, #NP0335BOX). Semi-dry transfer was performed using the Trans-Blot Turbo System (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... Equal amounts of lysed extracts were loaded on a NuPAGE 4-12% Bis-Tris Gel (Invitrogen; Carlsbad, CA) and transferred to a polyvinylidene difluoride (PVDF ...
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MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylationbioRxiv - Molecular Biology 2023Quote: ... Samples were resolved in NuPAGE® 4-12% Bis-Tris gels using MES buffer (Invitrogen, Life Technologies, # NP0321) and transferred to a 0.45 µM nitrocellulose membrane (GE Healthcare) ...
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MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylationbioRxiv - Molecular Biology 2023Quote: ... Samples were resolved in NuPAGE® 4-12% Bis-Tris gels using MES buffer (Invitrogen, Life Technologies, # NP0321) and transferred to a 0.45 µM nitrocellulose membrane (GE Healthcare) ...
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bioRxiv - Immunology 2023Quote: ... at 95°C for 5 min and separated on a 4-12% Bis-Tris gel (NuPAGE, Thermo Scientific) for 60 min at 170V ...
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bioRxiv - Neuroscience 2023Quote: ... Equal amounts of proteins for each sample were separated on 4-12% Bolt Bis-Tris Plus gels (ThermoFisher) and then transferred to a PVDF membrane (0.45 µm pore size ...
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bioRxiv - Neuroscience 2023Quote: ... On day 0, medium was replaced with neuronal medium (NM: DMEM/F-12, 1% N2-supplement (Thermo Fisher), 2% B27-supplement (Thermo Fisher) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were grown in DMEM / F-12 (1:1) + 2.5 mM L-glutamin + 10% FBS (Thermofisher Scientific, USA) + 1% penicillin / streptomycin (Thermofisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: Ten micrograms of protein was loaded onto a 4-12% Bis-Tris precast NuPage Gel (Thermo Fisher Scientific). Gel electrophoresis was performed at 140 V for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... and 5% 2-mercaptoethanol] and was resolved by SDS-PAGE on 4% to 12% Bis-Tris gels (Invitrogen). Proteins were transferred to a polyvinylidene difluoride immobilon TM-P membrane (Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... FIP5-mcherry expressing RCTE stable cells were cultured in delta TPG dish (12-071-33, ThermoFisher Scientific Inc.) with 0.5 ml 10% FBS containing medium ...
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bioRxiv - Cancer Biology 2023Quote: ... This insert creates a clean cell-free gap in a 6-or 12-well plate (Thermo Scientific, 150239). The assay was performed according to the manufacturer’s protocol (ibidi) ...
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bioRxiv - Cell Biology 2023Quote: ... plus 0.75 mg/mL collagenase (Roche, #11213873001, dissolved at 12 mg/mL in HBSS without calcium – Gibco, #14175095) for 8 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... and 20µg loaded into each well of a NuPAGE 4-12% Bis-Tris Gel (Invitrogen, #NP0335BOX, Carlsbad, CA). Gels were transferred to a membrane using the iBlot2 system (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... 2 x 5 min with 0.05 M PB and mounted on Superfrost Plus (ThermoFisher Catalog# 12-550-15) microscope slides ...
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bioRxiv - Microbiology 2023Quote: ... Macrophages plated in 12-well plate were lysed by incubating NP40 buffer (Thermo Fisher Scientific, Cat# J60766.AP) on ice for 30 min and protein concentration was measured by Bradford assay ...
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bioRxiv - Microbiology 2023Quote: ... at 65°C for 10 mins and resolved on a NuPAGE 4–12% Bis-Tris PAGE gel (Invitrogen) at 120 V for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... MPXV was passaged two times by inoculating a confluent layer of VeroE6 cells with a multiplicity of infection of 0.1-0.01 in Advanced DMEM/F-12 (Gibco) supplemented with 10 mM HEPES ...
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bioRxiv - Immunology 2023Quote: ... and 40 μg of each protein lysate were resolved on 4-12% Bis-Tris gels (Thermo Fisher, NP0322BOX) under denaturing conditions ...
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bioRxiv - Neuroscience 2023Quote: ... 35 µg of total protein was loaded onto a 4-12% (w/v) Bis-Tris polyacrylamide gel (Invitrogen) and electrophoresed before being electroblotted to a nitrocellulose membrane ...
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bioRxiv - Systems Biology 2023Quote: ... were loaded into the specified lanes of a Bolt 4-12% Bis-Tris Plus gel (Invitrogen™ #NW04125BOX) in Bolt™ MES SDS Running Buffer (Invitrogen™ #B0002) ...
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bioRxiv - Neuroscience 2023Quote: ... incubated for 5 min at 95°C and loaded onto a NuPAGE 4-12% Tris/Bis gel (ThermoFisher) with NuPAGE MOPS running buffer (ThermoFisher) ...
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bioRxiv - Immunology 2023Quote: ... Expression of GOI protein (Figure 2) was determined by SDS–PAGE using 4–12% gradient polyacrylamide gels (Invitrogen) and Coomassie staining and by Western blotting using antigen-specific sera.
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bioRxiv - Biochemistry 2023Quote: ... Degradation over time was visualized by running time points on a 4–12% Bis-Tris NuPAGE gel (Invitrogen) and staining with InstantBlue Coomassie Stain (Abcam).
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bioRxiv - Bioengineering 2023Quote: ... all components that were placed in an incubator were autoclaved (125 ℃ for 30 min) DMEM:F-12 (Gibco, 11320033) formulated with 10 % fetal bovine serum and 1 % pen/strep was employed as a cell culture media ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were boiled for 5 minutes then electrophoresed in pre-cast 4-12% NuPAGE Bis-Tris Gels (Invitrogen). Following transfer ...
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bioRxiv - Molecular Biology 2022Quote: ... 20μg total protein were loaded on SDS-PAGE precast gradient gels (4–12% Nu-Page Bis-Tris, Invitrogen), followed by transfer to nitrocellulose membrane ...
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bioRxiv - Molecular Biology 2022Quote: ... collected in 50 μl fractions and analyzed by SDS-PAGE (NuPAGE™ 4 to 12%, Bis-Tris, Invitrogen) run in MES-SDS buffer at 190V for 50 minutes.
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bioRxiv - Microbiology 2022Quote: ... Supernatants from cell lysates were run on 4–12% NuPAGE Bis Tris precast gels (Life Technologies, Beverley, MA) using MES running buffer (Life Technologies ...
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bioRxiv - Physiology 2022Quote: ... Western blotting was performed with whole-cell lysates run on 4-12% Bis-Tris NuPage gels (Life Technologies) and transferred onto Immobilon-P Transfer Membranes (Millipore) ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were resolved by SDS-PAGE electrophoresis on NuPAGE™ 4-12% Bis-Tris gels (Thermo Fisher Scientific) and MES SDS running buffer (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2024Quote: Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was conducted using 4 - 12 % NuPAGE gels (Thermo Fisher Scientific). Prior to SDS- PAGE ...
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bioRxiv - Bioengineering 2024Quote: ... a ‘shim’ photomask reflecting the geometry of a standard 1’’ x 3’’ microscope slide (12-559-A3, ThermoFisher) was printed at 20,000 d.p.i (CAD/Art Services ...
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bioRxiv - Cancer Biology 2024Quote: ... These tumor cells were cultured on dishes with the tumor 2D medium (Advanced DMEM/F-12 medium; Gibco, Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... equal volumes of viral lysates separated by electrophoresis on Bolt 4–12% Bis-Tris Plus gels (Life Technologies) were transferred using Trans-Blo Turbo Mini PVDF Transfer Packs (Bio-Rad) ...
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bioRxiv - Biophysics 2024Quote: ... Protein samples were separated using pre-cast 4-12% Bis Tris Protein Gels (Thermo-Fisher Scientific, Waltham MA) for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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Tuning non-linear mechanics in collagen hydrogels modulates cellular morphotypes in three dimensionsbioRxiv - Biophysics 2024Quote: ... Note that for cell culture experiments we used 12 µL a sterile solution of 7.5% sodium bicarbonate (Gibco) to neutralise the collagen solution to pH 7 ...