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Citations for Thermo Fisher :
5101 - 5144 of 5144 citations for HCCS Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: Protein identification by LC-MS/MS analysis of peptides was performed using an Orbitrap Fusion Lumos Tribid mass spectrometer (Thermo Fisher Scientific, San Jose, CA) interfaced with an Ultimate 3000 Nano-HPLC apparatus (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2019Quote: Nanoflow LC–MS/MS analysis of tryptic peptides was conducted on a quadrupole Orbitrap mass spectrometer (Q Exactive HF-X, Thermo Fisher Scientific, Rockford, IL, USA) [48] coupled to an EASY nLC 1200 ultra-high-pressure system (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... MS spectra and MS/MS spectra were acquired from eluting peptides ionized with electrospray ionization (ESI) and analyzed in a LTQ Orbitrap Velos hybrid mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA), as described previously (Verberkmoes et al. ...
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bioRxiv - Neuroscience 2020Quote: ... The tryptic peptides were separated and analyzed using a Dionex Ultimate 3000 RSLCnano system coupled to a Q Exactive instrument (Thermo Fisher Scientific, San Jose, CA) as previously described (Gallart-Palau et al. ...
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bioRxiv - Cancer Biology 2021Quote: Peptides from all experiments were separated by nanoscale C18 reverse-phase liquid chromatography using an EASY-nLC II 1200 (Thermo Fisher Scientific, Waltham, MA, USA), using a binary gradient with buffer A (2% acetonitrile ...
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bioRxiv - Molecular Biology 2020Quote: ... The tryptic peptides were loaded at 5 μL/min onto a C18 trap column (Thermo Scientific, 100 µm ID×2 cm, 5 μm, 120 Å). Peptides were separated with PicoFrit columns (New Objective ...
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bioRxiv - Biophysics 2020Quote: ... The eluted peptides were analyzed in a mass spectrometer with an orbitrap mass analyser (Orbitrap Fusion™ mass spectrometer, Thermo Scientific, San Jose, California, USA).
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bioRxiv - Neuroscience 2021Quote: ... Briefly, peptides were loaded onto a Thermo NanoViper trap column (75 µm x 20 mm, 3 µm C18, 100 Å) (Thermo Fisher Scientific, Bremen, Germany) using an Agilent 1260 Infinity nanoLC system (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... using an EASY-nLC 1200 system (peptides were loaded and separated on a 30 cm long home-made C18 column; Thermo Fisher Scientific, Waltham, MA, USA) coupled to a Q Exactive Plus system (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2021Quote: ... Four μl of the different peptide mixtures (800 ng for yeast peptides and 949 ng for bulks) were analyzed using an Orbitrap Fusion™ Lumos™ Tribrid™ mass spectrometer (Thermo Fisher Scientific).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... these peptides were loaded onto the Xbridge BEH300 C18 column (Waters Milford, MA, USA) for HPLC (UltiMate 3000 UHPLC; Thermo Fisher Scientific, Waltham, MA, USA) separation ...
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bioRxiv - Biochemistry 2021Quote: ... For the FAIMS CV scanning of synthetic peptides and glycopeptides, each sample (Table S1, Supporting Information) was directly injected to the Orbitrap Lumos (Thermo Fisher Scientific, San Jose, CA). The CV was scanned from 0-100 V in 1 V steps ...
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bioRxiv - Cancer Biology 2021Quote: ... The eluted peptides were analyzed in a mass spectrometer with an orbitrap mass analyser (Orbitrap Fusion™ mass spectrometer, Thermo Scientific, San Jose, California, USA).
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bioRxiv - Microbiology 2019Quote: ... Samples were resuspended in 20 µL 0.1 % formic acid (FA) and peptides were quantified by measuring the absorbance at 280 nm using a Nanodrop 1000 device (Thermo Fisher Scientific, Rockford, IL, USA).
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFUSE-vector (Invitrogen, Thermo Fischer Scientific, MA, USA). The human ACE2 was expressed by essentially the same protocol used for the COVID-19 RBD ...
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bioRxiv - Immunology 2020Quote: ... Acidified eluted peptides were analyzed in the data-independent acquisition mode on a Q Exactive HF-X mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) online coupled to an ultra-high-performance liquid chromatography (UHPLC ...
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bioRxiv - Cell Biology 2022Quote: ... One aliquot containing 800 ng of Bradford-quantified peptides was analyzed on an ETD-enabled Orbitrap Velos mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) connected to the EASY-nLC system (Proxeon Biosystem ...
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bioRxiv - Neuroscience 2022Quote: ... labeled peptides on one spin tip from a High-Select™ TiO2 Phosphopeptide Enrichment Kit (capacity of 1–3 mg; Thermo Fisher Scientific, catalog A32993). After preparing spin tips ...
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bioRxiv - Genetics 2022Quote: ... Sodium deoxycholate was removed by acidification with 10 % TFA and the sets were desalted using Pierce peptide desalting spin columns (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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Multiomic Approach Characterises the Neuroprotective Role of Retromer in Regulating Lysosomal HealthbioRxiv - Neuroscience 2022Quote: ... the resulting peptides were labelled with Tandem Mass Tag (TMT) ten plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific, Loughborough, LE11 5RG, UK) and the labelled samples pooled.
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bioRxiv - Pharmacology and Toxicology 2022Quote: The eluted peptides were ionized under high voltage (2 kV) and analyzed online using a LumosTM mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA). The initial MS spectrum (MS1 ...
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bioRxiv - Cell Biology 2023Quote: Peptides were analyzed by LC-MS on a Dionex Ultimate HPLC coupled to an Orbitrap QE Orbitrap mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) operating in Data Dependent Acquisition (DDA ...
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bioRxiv - Cell Biology 2022Quote: ... 1 µg of the peptide was used for LC-MS/MS analysis which was carried out using a nano-LC 1200 system (Thermo Fisher Scientific, San Jose, CA) coupled to Orbitrap Fusion™ Lumos ETD mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were separated on a 25 cm EasySpray™ C18 column (2 μm, 100 Å, 75 μm x 25 cm, Thermo Scientific Cat No: ES902A) at 400 nL/min with a gradient of 4-30% with mobile phase B (Mobile phases A ...
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bioRxiv - Cancer Biology 2023Quote: ... we used the modified pSec-NtermHis6 vector containing secretory signal peptide (IgK Leader) as found originally in commercial vector pSecTag/FRT/V5- His-TOPOR vector (Thermo Fisher Scientific, Waltham, MA, USA), but fused to six histidine residues (His6 ...
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bioRxiv - Cancer Biology 2023Quote: ... Tryptic peptides from protein digests obtained from cell lines and tissue lysates were quantified by BCA using a peptide digest standard (Thermo Scientific, catalog no. 23225; 23295). Peptides were analyzed by an Orbitrap Eclipse Tribrid mass spectrometer (Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... MS spectra and MS/MS spectra were acquired from eluting peptides in a LTQ Orbitrap Velos hybrid mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA), as described previously [77 ...
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bioRxiv - Immunology 2023Quote: ... Protein identification by LC-MS/MS analysis of peptides was performed using an Orbitrap Fusion Lumos Tribid mass spectrometer (Thermo Fisher Scientific, San Jose, CA) interfaced with an Ultimate 3000 Nano-HPLC apparatus (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Mixed peptide samples were prepared from 6 µL of each sample and separated by high pH reversed phase peptide separation kit (Thermo Fisher Scientific, Waltham, MA, USA). Ten fractions were collected by centrifugation ...
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bioRxiv - Cell Biology 2022Quote: ... Blocking Buffer (E-cadherin mAb #14472 Cell Signaling Technology 1:1000, β-catenin mAb #8480 Cell Signaling Technology 1:1000, Occludin mAb #33-1500 Invitrogen 1:500). On the next day the membranes were washed three times for tenminutes in Tris-buffered saline + 0.05% Tween (#91414 ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were washed three times in PBS and incubated during 4 h in a blocking solution containing a secondary antibody to reveal TH (donkey anti-rabbit Alexa Fluor 594, Invitrogen A21207 lot 1890862 and 2145022 (1:400), RRID ...
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bioRxiv - Molecular Biology 2022Quote: ... The slips were washed three times with PBS before the complementary anti-rabbit secondary antibody was added (Cat#A-11034, Life Technologies; 1:1000 dilution in blocking solution) and incubated for one hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... We then incubated membranes with secondary antibody (in 5% Blocking Buffer) for 1 hour at room temperature (Invitrogen cat. A16110; Invitrogen cat. 31432; Invitrogen cat. 31402; R&D Biosystems cat. HAF016). We performed a final TBST wash (4 x 10 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... TFA and 1 μL of this dilution and 1μL of the undiluted peptides from bands 3 and 4 were injected onto the trapping column (Thermo Scientific, PepMap100, C18, 300 μm × 5 mm), using a partial loop injection ...
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bioRxiv - Biochemistry 2021Quote: Samples from imaging peptide extraction were dissolved in 0.1% FA and analyzed on the Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer (Thermo Fisher Scientific, Bremen, Germany) coupled to a Dionex UPLC system ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides (100 ng of acyl-BSA) were loaded onto an Acclaim PepMap100 C18 trap column (Thermo Scientific, Product #16-494-6, 75μm x 2cm, 100 Å) and separated on an Acclaim PepMap RSLC C18 analytical column (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2022Quote: ... and the peptides were fractionated with high pH RP method and analyzed by a Q Exactive™ HF-X mass spectrometer (Thermo Fisher Scientific, San Jose, CA) coupled with an online HPLC.
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bioRxiv - Neuroscience 2022Quote: ... One fifth of the phosphopeptides and one tenth of each global peptide fraction was loaded onto a reversed phase EasySpray™ C18 column (2 μm, 100 Å, 75 μm × 25 cm, Thermo Scientific Cat No: ES902A) at 400 nL/min ...
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bioRxiv - Microbiology 2023Quote: ... The separated peptides were ionized by a nano-ElectroSpray Ionization (ESI) and transferred to an Orbitrap Exploris™ 480 mass spectrometer (Thermo Fisher Scientific, San Jose, CA) for detection in DDA (Data dependent Acquisition ...
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bioRxiv - Neuroscience 2022Quote: ... washed with PBS-T and then incubated with secondary antibodies for 2h (donkey anti-rabbit lgG Alexa Fluor 488, Invitrogen, Thermo Fisher, A21206; donkey anti-Mouse lgG Alexa Fluor 555, Invitrogen, Thermo Fisher, A21202, 1:600 in blocking buffer). Sections were washed twice with PBS before being mounted onto gelatin subbed slides ...
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bioRxiv - Cancer Biology 2020Quote: The DDA acquisition of peptides from cell lines was performed on a nanoflow a nanoflow EASY-nLC™ 1200 System (Thermo Fisher Scientific™, San Jose, USA) coupled to a Q Exactive HF-X hybrid Quadrupole-Orbitrap (Thermo Fisher Scientific™ ...
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bioRxiv - Physiology 2023Quote: ... samples were incubated with secondary antibodies diluted 1:500 and DAPI (1:1000) in blocking/permeabilisation buffer (Dk-a-Ch-AF488 – 703-545-155 Jackson Immunoresearch, Dk-a-Rb-AF546 – A10040 Thermofisher, Dk-a-Gt-AF647 – A-21447 Life Tech) for 1h at room temperature ...
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bioRxiv - Biochemistry 2020Quote: On the Orbitrap setup comprised of a Dionex Ultimate 3000 nano LC with Thermo Q Exactive Classic peptides were separated on a 50-cm EasySpray column (Thermo Fisher, ES803, 2 μm beads, 75-μm ID) with a 60 minute gradient of 2 to 35% acetonitrile in 0.1% formic acid and 5% DMSO at a flow rate of 250 nL/min.
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bioRxiv - Cancer Biology 2022Quote: ... for 24h at 4°C on a shaker and then stained with antibodies (rat anti-CD3 [clone 17A2, cat#100202, Biolegend]; goat anti-myeloperoxidase [goatMPO, AF3667, R&D Systems], and [hamsteranti-CD31, 2H8, MA3105, Thermo Fisher] all diluted 1:200 in blocking buffer), for 3 days at 4°C on a shaker ...