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Citations for Thermo Fisher :
4801 - 4850 of 5144 citations for HCCS Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Each fraction of tryptic peptides was injected and separately analysed on an Ultimate 3000 HPLC system coupled to a QExactive Orbitrap (Thermo Fisher Scientific). The trap (5 mm × 300 µm ...
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bioRxiv - Microbiology 2023Quote: ... 100 μg of protein from each condition was subjected to in-solution digestion before labelling the resultant peptides using the TMT-10plex Isobaric Label Reagent Set (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Eluting peptides were ionized by electrospray ionization and analyzed in a Q Exactive HF hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific) with the following parameters ...
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bioRxiv - Molecular Biology 2023Quote: Phosphoribosylated peptides were analyzed on the EASY-nLC 1200 coupled to an Orbitrap Eclipse Tribrid mass spectrometer (ThermoFisher Scientific, San Jose, CA). Peptides were separated on an Aurora UHPLC Column (25 cm × 75 μm ...
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bioRxiv - Neuroscience 2023Quote: ... Peptides were eluted from the S-Traps with 0.2% formic acid in 50% aqueous acetonitrile and quantified using Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2023Quote: Peptides in the compiled sample were fractionated (8 fractions) using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Cat: 84868; Thermo Fisher Scientific). Prior to liquid chromatography–mass spectrometry (LC-MS ...
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bioRxiv - Biochemistry 2023Quote: ... Dried samples were redissolved with 300 μL of 0.1% TFA in H2O and further fractionated using high-pH reversed-phase peptide fractionation kits (ThermoFisher, P/N 84868) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: LC-MS/MS of pTyr peptides was carried out on an Agilent 1260 LC coupled to a Q Exactive HF-X mass spectrometer (Thermo Fisher Scientific). Peptides were separated using a 140-minute gradient with 70% acetonitrile in 0.2 mol/L acetic acid at flow rate of 0.2 mL/minute with approximate split flow of 20 nL/minute ...
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bioRxiv - Cancer Biology 2023Quote: ... The peptide separation for the phosphoproteome analysis was carried out with an Acclaim™ PepMap™ 100 C18 column (Thermo Fisher Scientific) using a 155min non-linear gradient from 3 to 35% of B (0 min ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting peptides were analyzed by online nano liquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive Plus, Thermo Fisher Scientific) using a 120 min gradient ...
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bioRxiv - Systems Biology 2023Quote: ... The acetonitrile was evaporated in a SpeedVac at 45°C and peptide concentration was determined by measuring absorbance at 280 nm on a NanoDrop 2000C spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2023Quote: ... The proteins were trypsinized and the resulting peptides were separated by HPLC and analyzed by LC-S/MS on Q-Exactive (Thermo Fisher Scientific). Data analysis was done as described in (Kurmanbayeva et al ...
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bioRxiv - Plant Biology 2023Quote: A data-dependent LC-MS/MS analysis was performed on the extracted peptide mixtures after digestion using a Fusion tri-hybrid orbitrap mass spectrometer (Thermo Fisher Scientific) interfaced through a nano-electrospray ion source to a RSLC 3000 HPLC system (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... These peptides were desalted using Oasis HLB cartridges (Waters, Milford, MA) and then dried by Speed Vac (Thermo Fisher Scientific, Bremen, Germany). Then ...
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bioRxiv - Plant Biology 2023Quote: Generated peptides were analyzed on a Dionex Ultimate 3000 RSLCnano system coupled to a Q-Exactive HF-X mass spectrometer (Thermo Fisher Scientific) in data-dependent acquisition mode ...
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bioRxiv - Plant Biology 2024Quote: ... 1 to 3 µl of each sample were subjected to reverse phase LC for peptide separation using an RSLCnano Ultimate 3000 system (Thermo Fisher Scientific). Peptides were loaded on an Acclaim PepMap 100 pre-column (100 µm x 2 cm ...
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bioRxiv - Microbiology 2024Quote: ... Peptides were loaded on a trapping column and eluted over a 75μm x 50cm analytical column (Thermo Fisher P/N ES-803) at 300 nL/min using a 1hr reverse phase gradient ...
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bioRxiv - Microbiology 2024Quote: ... Extracted and desalted [45] peptides were analysed on an Easy-nLC 1200 system coupled to a Q Exactive HF mass spectrometer (all Thermo Fisher Scientific) [44] ...
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bioRxiv - Cancer Biology 2023Quote: ... dried peptides were resuspended in LC buffer and subjected to DDA-MS on a QExactive H-FX mass spectrometer (Thermo Fisher Scientific) equipped with an Easy-nLC 1200 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... An equal amount of each peptide sample was loaded into an Easy-nLC 1000 nano-LC system (Thermo Scientific, Waltham, MA, USA) coupled with a quadrupole Orbitrap mass spectrometer (Q ExactiveTM ...
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bioRxiv - Physiology 2024Quote: ... Liquid Chromatography with tandem mass spectrometry analysis of the digested labeled peptides was then carried out employing an EASY-nLC 1000 UPLC system (Thermo Fisher Scientific) incorporating a 50 cm EASY-Spray Column coupled with an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides were eluted on an Acclaim PepMap 100 C18 analytical column (75 µm × 50 cm, 3 µm particle size; Thermo Fisher Scientific) at 300 nL/min using the following gradient of solvent B ...
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bioRxiv - Cell Biology 2024Quote: ... Tryptic peptides (2 µg) were loaded onto a C18 trap (75 µm × 2 cm; Acclaim PepMap 100, P/N 164946; Thermo Fisher Scientific) at a flow rate of 2 µL/min of solvent A (0.1% formic acid and 3% acetonitrile in LC-mass spectrometry grade water) ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive HF, Thermo Fisher Scientific). For this purpose ...
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bioRxiv - Cancer Biology 2024Quote: ... Approximately 0.5 µg peptides per sample were automatically loaded to the online coupled ultra-high-performance liquid chromatography (UHPLC) system (Ultimate 3000, Thermo Fisher Scientific). A nano trap column was used (300-µm ID X 5 mm ...
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bioRxiv - Cell Biology 2024Quote: Processed peptides were analysed by nanoflow liquid chromatography mass spectrometry (LC-MS) using an EASY-nLC 1000 Liquid Chromatograph (Thermo Fisher Scientific) connected to a custom-made 30-cm capillary emitter column (75μm inner diameter ...
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bioRxiv - Neuroscience 2024Quote: LC-MS/MS analysis of the resultant peptides was performed on an UHPLC connected to a Q-Exactive Orbitrap mass spectrometer (Thermo Fisher Scientific) through a nano electrospray ion source (AMR Inc. ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were desalted for 4 minutes with Thermo Scientific POROS R2 reversed-phase resin (Thermo Scientific POROS R2 reversed-phase resin 1112906) hand packed into a trap column (1 mm ID × 2 cm ...
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bioRxiv - Biochemistry 2024Quote: Whole proteome: Eluting peptides were ionized in a nanoESI source and on-line detected on a QExactive HF mass spectrometer (Thermo Fisher Scientific). The mass spectrometer was operated in a TOP10 method in positive ionization mode ...
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bioRxiv - Biochemistry 2024Quote: ... Mixed peptide samples were prepared from 4 μL of each sample and separated using a high pH reversed-phase peptide separation kit (Thermo Fisher Scientific) according to the instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were loaded onto a trap column (PepMap Acclaim C18, 5 mm × 300 μm ID, 5 μm, 100 Å, Thermo Fisher Scientific) with loading buffer (100% water ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were loaded onto a trap column (PepMap Acclaim C18, 5 mm × 300 μm ID, 5 μm, 100 Å, Thermo Fisher Scientific) with loading buffer (100% water ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were loaded onto a trap column (PepMap Acclaim C18, 5 mm × 300 μm ID, 5 μm, 100 Å, Thermo Fisher Scientific) with loading buffer (100% water ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were loaded onto a trap column (PepMap Acclaim C18, 5 mm × 300 μm ID, 5 μm, 100 Å, Thermo Fisher Scientific) with loading buffer (100% water ...
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A conserved germline-specific Dsn1 alternative splice isoform supports oocyte and embryo developmentbioRxiv - Cell Biology 2024Quote: 1 µg trypsinized peptides were dissolved in 50 mM TEAB pH 8.5 and labelled using the TMT10plex Isobaric Labeling Reagent Set (Thermo Fisher Scientific, 90111). Each sample was labeled with TMT10plex reagents at a 20:1 label:peptide w/w ratio ...
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bioRxiv - Systems Biology 2024Quote: ∼800 ng of digested and purified peptide mixture was loaded onto an LC-MS/MS system consisting of an EASY-nLC 1000 nanoflow liquid chromatograph (Thermo Fisher Scientific) coupled with either a Q Exactive HF-X mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... of samples were pooled and separated into 6 fractions by off-line basic reversed-phase (bRP) using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific). The fractions were collected in 7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... peptides were concentrated using a PepMap100 C18 pre-column (75 µm x 2 cm, with 3 µm particle size; Thermo Fisher Scientific) and subsequently resolved on an EASY-Spray column (ES903 ...
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bioRxiv - Microbiology 2024Quote: ... The peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive HF, Thermo Fisher Scientific) using a 180 min gradient for fractions and a 480 min gradient if no fractionation was performed ...
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bioRxiv - Immunology 2024Quote: ... mice were vaccinated with 100 µl of either 1 x 107 CFU of BCG in PBS or with 10 µg P25 peptide in PBS formulated with one of the following adjuvants: 1:1 volume ratio of alum (Imject Alum; Thermo Fisher Scientific), or 5% final volume of LASTS-C [Span85-Tween 80-squalene ...
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bioRxiv - Microbiology 2024Quote: The digested peptides were analyzed by nanoflow reverse-phase LC followed by tandem MS using a Q Exactive hybrid mass spectrometer (Thermo Fisher Scientific). The capillary reverse-phase HPLC-MS/MS system was composed of a Dionex U3000 gradient pump equipped with a VICI CHEMINERT valve ...
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bioRxiv - Immunology 2024Quote: ... Eluted peptides were dried overnight before being resuspended in 50 µl 1% formic acid and peptide concentration measured using the CBQCA assay following manufacturers protocol (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2024Quote: ... One µg of peptide was loaded onto a PepMap 100 C18 trap column (75 µm id x 2 cm length; Thermo Fisher Scientific) at 3 µL/min for 10 min with 2 % acetonitrile (volume fraction ...
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bioRxiv - Biochemistry 2020Quote: ... Secondary antibody probing was for 1 hour in the dark with the following antibodies at 1:1000 in blocking buffer: goat anti-rabbit Alexa Fluor 594 (Life Technologies, Carlsbad CA, A11037) and goat anti-mouse Alexa Fluor 488 (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were rinsed in PBS 3 times for 5 min and then incubated for 2 h at RT in blocking solution with the corresponding Alexa-conjugated secondary antibodies (Thermo Fisher Scientific; Massachusetts, USA). Finally ...
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bioRxiv - Molecular Biology 2021Quote: ... The secondary Alexa Fluor 546 donkey anti-rabbit IgG and Alexa Fluor 488 donkey anti-mouse IgG (Invitrogen, 1:500, diluted in blocking solution) antibodies were added together with 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Bioengineering 2020Quote: ... bioengineered intestinal tubules were incubated with zonula occludens-1 (ZO-1) antibody (1:1000 diluted in blocking solution) (Thermo Fisher Scientific, Bleiswijk, The Netherland) for 2h ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated overnight at 4°C in blocking solution (PBS, 2% normal goat serum, 3% BSA) and primary antibodies (mouse anti-HA, Invitrogen; rabbit anti-GFP, Invitrogen). Cells were then immunolabeled with Alexa-conjugated secondary antibodies (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... permeabilized with 0.1% Triton X-100 in PBS for 10 min and blocked with 2% BSA solution in PBS for 1 h followed by overnight incubation at 4°C with primary antibodies diluted in 2% BSA blocking solution: mouse monoclonal [1F7G5] anti-CKS2 (1:100 dilution; Thermo Fisher Scientific; 37-0300), rabbit polyclonal anti-P63 (p63α ...
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bioRxiv - Bioengineering 2020Quote: ... and secondary antibodies prepared in blocking solution were applied for 2 hours at room temperature: donkey anti-mouse 568 (Alexa Fluor® 568, 1:500, Thermo Scientific Cat# A10037) and donkey anti-rabbit 647 (Alexa Fluor® 647 ...