Labshake search
Citations for Thermo Fisher :
5051 - 5100 of 5144 citations for HCCS Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2019Quote: ... The peptides were desalted by an HLB column (Waters, Milford, MA) and were then drained by a vacuum pump (Thermo Fisher Scientific, Bremen, Germany). The peptides were acidified with 0.1% formic acid and were loaded onto a reversed-phase microcapillary column using a Thermal EASY-n LC1200 chromatography system ...
-
bioRxiv - Cell Biology 2020Quote: Nanoflow LC–MS/MS analysis of tryptic peptides was conducted on a quadrupole Orbitrap mass spectrometer (Q Exactive HF-X, Thermo Fisher Scientific, Bremen, Germany)14 coupled to an EASY nLC 1200 ultra-high-pressure system (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... Digestion was stopped by the addition of 2% (v/v) formic acid before peptides were cleaned using Pierce C18 spin columns (Thermo Fisher Scientific, cat#89870) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2021Quote: ... Peptides were then directly labeled as follows: acetonitrile was added to 30% (v/v) concentration and peptides were labeled with TMT 10-plex reagent (Thermo Fisher Scientific, Catalog #90406) for 1 hour ...
-
bioRxiv - Physiology 2020Quote: ... Antibodies to the antigen peptide were purified from the final bleed antiserum by affinity-purification using the AminoLink Plus Immobilization Kit (ThermoFisher Scientific, Waltham, MA, USA), with bound antibodies eluted using glycine elution buffer [6.3 ml of 100 mM glycine (VWR Chemicals ...
-
bioRxiv - Genetics 2021Quote: ... The separated peptides were loaded onto a C18 column (50 cm × 75 μm) connected to an EASY-nLC™ 1200 system (Thermo Fisher Scientific, USA) and separated with a linear gradient of buffer B (80% acetonitrile and 0.1% formic acid ...
-
bioRxiv - Biochemistry 2021Quote: Separation of digested histone peptides was performed by using nanoliter flow liquid chromatography using an EASY-nLC nanoHPLC (Thermo Scientific, San Jose, CA, USA) equipped with an analytical weak cation exchange–hydrophilic interaction liquid chromatographic resin (PolycatA ...
-
bioRxiv - Developmental Biology 2021Quote: ... One microgram of peptide was injected into an Ultimate 3000 RSLC chromatography system connected to a Q Exactive HF-X mass spectrometer (Thermo Scientific, Waltham, MA, USA). Samples were transferred to a PepMap 100 C18 trap column (100 µm x 2 cm ...
-
bioRxiv - Biochemistry 2020Quote: Peptides and cross-linked peptides were analyzed using an UltiMate3000 RSLCnano (Dionex, Sunnyvale, CA) coupled to an Orbitrap Fusion (Thermo-Fisher Scientific, San Jose, CA) mass spectrometer equipped with a nanospray Flex Ion Source ...
-
bioRxiv - Developmental Biology 2022Quote: ... The digested peptides were desalted using Oasis HLB cartridges (Waters, Milford, MA, USA) and then dried by vacuum evaporation (Thermo Fisher Scientific, Bremen, Germany).
-
bioRxiv - Microbiology 2024Quote: ... followed by a peptide clean-up on SPE spin columns before TMT labeling with the TMTpro™ 16plex Label Reagent Set (Thermo Fisher, Cat# A44520). Peptides from each sample were labeled with a distinct TMTpro™ isobaric tag before mixing ...
-
bioRxiv - Cancer Biology 2024Quote: Tandem Mass Tag (TMT)-10 labeling of peptides was conducted as follows: The TMT-10 plex Isobaric Label Reagent (Catalog No. 90110, Thermo Fisher Scientific, Waltham, MA) was dissolved in anhydrous acetonitrile (ACN ...
-
bioRxiv - Molecular Biology 2022Quote: ... and fragmented to produce a tandem mass-spectrum of specific fragment ions for each peptide with LTQ Orbitrap Velos Pro ion-trap mass spectrometer (Thermo Fisher Scientific, Waltham, MA).
-
bioRxiv - Immunology 2022Quote: ... the RBD was inserted into pcDNA3.4 with a streptavidin binding peptide (SBP) tag at the C-terminus and were produced using the Expi293 Expression System (Thermo Fisher Scientific, Waltham, MA) according to manufacturer’s instruction ...
-
bioRxiv - Cancer Biology 2024Quote: ... Tryptic peptides extracted from each IP product were separated by reverse-phased nano-flow liquid-chromatography (EASY-nLC II, Thermo Scientific, San Jose, CA) and analyzed on an LTQ/Orbitrap Velos Elite hybrid mass spectrometer (Thermo-Fisher ...
-
bioRxiv - Neuroscience 2024Quote: ... Peptides were loaded onto a C18 pre-column (3 μm, 75 mm i.d. × 2 cm, 100 Å, Thermo Fisher Scientific Cat# 16-494-6) then separated on a reverse-phase nano-spray column (2 μm ...
-
bioRxiv - Neuroscience 2024Quote: LC-MS/MS analysis for DDA Peptides from each pooled fraction were first spiked with iRT standard peptides (Biognosys AG, Switzerland) and separated using reverse-phase high-performance liquid chromatography (RP-HPLC) on EASY-nLC system (Thermo Fisher Scientific, Bremen, Germany) with a column (75 μm × 150 mm ...
-
bioRxiv - Immunology 2023Quote: ... Direct pepscan analysis was undertaken by binding 200 ng of each overlapping peptide to Nunc MaxiSorp 96 well ELISA plates (Thermo Fisher Scientific, Waltham, MA) overnight at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... The peptide amount of tumor cell samples was estimated using a fluorescence based protein assay (Qubit Protein assay, Thermo Fisher Scientific, Waltham, MA, USA).
-
bioRxiv - Cell Biology 2023Quote: ... each peptide sample (approximately 2 mg) was tapplied to a Pierce High-Select™ TiO2 Phosphopeptide Enrichment Kit (Thermo Fisher Scientific, Cat No: A32993). After preparing spin tips as per the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... peptides were reconstituted in 0.1% trifluoroacetic acid and fractionated on Sep-Pak® Vac cartridges using methodology and reagents from Pierce™ High pH reversed-phase peptide fractionation kit (8 fractions for global proteomics and 4 for phosphoproteomics skipping every other; Thermo Fisher Cat No: 84868). Samples were run (1/8th of each global and 1/5th of each phosphopeptide fraction ...
-
bioRxiv - Cell Biology 2023Quote: Peptide concentrations of whole cell S-trap extracts in 100 mM TEAB were quantified using the Pierce Quantitative Fluorometric Peptide Assay (Thermo Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... Eluted peptides were subjected to electrospray ionization and then entered into an LTQ Orbitrap Velos Elite ion-trap mass spectrometer (Thermo Fisher Scientific, Waltham, MA). Peptides were detected ...
-
bioRxiv - Cancer Biology 2023Quote: ... the peptides were analyzed by online nanoflow liquid chromatography-tandem mass spectrometry performed on an EASY-nanoLC 1200 system (Thermo Fisher Scientific, MA, USA) connected to a Q Exactive™ Plusmass spectrometer (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2023Quote: ... and washed for 3 min at 30 μL/min before peptides were eluted onto a C18 Acclaim™ PepMap™ column (100 Å, 5 μm × 0.75 mm × 150 mm, Thermo Fisher Scientific) at a flow rate of 0.3 μL/min ...
-
bioRxiv - Biochemistry 2023Quote: ... as three full experimental technical replicates (n=54) with ∼100 ng of peptides injected for each chromatographic run using a Dionex UltiMate 3000 uHPLC system (Thermo Fisher Scientific, Bremen, Germany). Solvent A was 1% CH3CN in 0.1% (v/v ...
-
bioRxiv - Cell Biology 2023Quote: For the identification of the peptides a Dionex Ultimate 3000 RSLCnano System coupled to an Orbitrap Eclipse Tribrid Mass Spectrometer (both Thermo Fisher Scientific, Vienna, Austria) was used.
-
bioRxiv - Microbiology 2023Quote: ... Peptides were loaded on an Acclaim PepMap 100 μ-precolumn cartridge (5 μm, 100 Å, 300 μm ID x 5 mm, Thermo Fisher Scientific) and separated at 40°C on a PicoTip emitter (noncoated ...
-
bioRxiv - Bioengineering 2023Quote: ... the peptides were separated and analyzed using a Dionex Ultimate 3000 RSLCnano system coupled to a Q Exactive instrument (Thermo Fisher Scientific, MA, USA). Separation was performed on a Dionex EASY-Spray 75 μm × 10 cm column packed with PepMap C18 3 μm ...
-
bioRxiv - Immunology 2023Quote: The inhibition of kinase activity mediated by phospholipids was determined using the following Z’-LYTE Kinase Assay Kits: Z’-LYTE Kinase Assay Kit - Ser/Thr 6 Peptide (Thermo Fisher Scientific, Cat# PV3179) used to measure AKT activity and the Z’-LYTE Kinase Assay Kit - Ser/Thr 7 Peptide (Thermo Fisher Scientific ...
-
bioRxiv - Biophysics 2024Quote: ... Degraded ProteaseMAXTM was removed via centrifugation (max. speed, 10 minutes) and the peptide solid phase extracted using 10 µl volume C18 tips (Pierce○R (Thermo Fisher Scientific, Waltham, MA) according to manufacturer protocol ...
-
bioRxiv - Neuroscience 2024Quote: ... the peptide mixture was split into two equal aliquots of 1 mg each and 350 fmol of standard AQUA QuantPro peptides (Thermo Fisher Scientific, Waltham, USA) were added to each aliquot before K-ε-GG peptide enrichment ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Peptides were loaded onto a C18 Acclaim PepMap NanoLC column (25 cm length, 75 µm inner diameter, Thermo Fisher Scientific, San Jose, CA, USA) packed with 2 µm particles and a 100 Å pore size ...
-
bioRxiv - Cell Biology 2022Quote: ... then incubated at room temperature for 1 hour in a dark humid chamber with goat anti- mouse IgG-Alexa488 (Invitrogen, A11001, 1:500 in blocking buffer). Cells were washed 4 more times with blocking buffer ...
-
bioRxiv - Cell Biology 2020Quote: ... sections were washed 2 times for 8 minutes in DPBS containing 0.3% Triton X100 and then incubated for 1 hour in blocking solution containing the secondary antibody (1/200, Invitrogen P10994 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody ...
-
bioRxiv - Neuroscience 2020Quote: ... and then incubated in secondary antibodies diluted with the blocking solution (1:100 for goat anti-mouse Alexa 633 (Thermo Fisher Scientific #A-21052, RRID: AB_2535719), 1:100 for goat anti-chicken Alexa 488 (Thermo Fisher Scientific #A-11039 ...
-
bioRxiv - Immunology 2022Quote: ... The cells were washed four times in PBS and incubated with goat anti-rabbit antibody conjugated with AlexaFluor594 for 1 hour at room temperature (Invitrogen; 1:200 dilution in blocking reagent). 4’,6-diamidino-2-phenylindole (DAPI ...
-
bioRxiv - Neuroscience 2019Quote: ... the cells were incubated with goat anti-rabbit or anti-mouse IgG antibody conjugated with Alexa Fluor 488 dye or Alexa Fluor 568 dye (Life Technologies; 1000x dilution in blocking solution) for 60 min at room temperature ...
-
bioRxiv - Microbiology 2021Quote: ... Triton-X-100 and 5% (v/v) normal goat sera) for 30 min prior to staining with a rabbit anti-HA polyclonal antibody (Invitrogen, diluted 1:100 in blocking buffer) for 1 h ...
-
bioRxiv - Microbiology 2022Quote: ... the cells were washed three times with the blocking buffer and incubated at room temperature with fluorophore-conjugated secondary antibodies (goat anti-mouse IgG Alexa 488, Thermo Fisher, Cat #: A11001, 1:500 dilution) (goat anti-rabbit IgG Alexa 555 ...
-
bioRxiv - Bioengineering 2024Quote: ... Blotting took place with the LI-COR system (LI-COR 927-66003 [TBS-based blocking and diluent buffers] and ThermoFisher 28360 TBS-Tween 20 wash buffer) for imaging on a LI-COR Odyssey instrument ...
-
bioRxiv - Cancer Biology 2024Quote: ... the mesenteries were incubated with a secondary antibody consisting of a blocking buffer solution and Alexa Fluor 488 goat anti-rabbit IgG (Thermo Fisher Scientific, A-11008, 1:1000) for 1 hour in a darkroom then washed 3 times with PBS for 10 minutes and mounted on microscopic slides ...
-
Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... before secondary antibody (Donkey α-Rabbit, Alexa Fluor 488, Invitrogen #A21206, 1:2000; Goat α- mouse, Alexa Fluor 488, Invitrogen #A11001, 1:2000 in blocking buffer) was applied to the sections and incubated for 1 h at RT ...
-
bioRxiv - Neuroscience 2023Quote: ... blocked in 3% normal donkey serum/0.25% Triton X100 in PBS for 1 h at room temperature and then incubated overnight at room temperature in blocking solution containing primary antiserum (rabbit anti-GFP, 488-conjugated, Novus #NB600-308, 1:5000; rabbit anti-TH, Thermo Fisher Scientific #OPA1-04050, 1:5000). The next morning ...
-
bioRxiv - Microbiology 2023Quote: ... Resulting biofilms were first washed with 1x PBS to remove loosely-bound cells and incubated with BSA blocking buffer at room temperature for 0.5 h (Thermo Scientific, 3% BSA is PBS; J61655.AK). Biofilms were then probed with two murine monoclonal antibodies against B-DNA (Anti-dsDNA ...
-
bioRxiv - Cell Biology 2023Quote: ... slides were incubated in the dark for 30 min at room temperature with secondary antibodies diluted 1:800 in blocking buffer: donkey anti-rabbit AlexaFluor 488 (Thermo Fisher Scientific, Catalog No. A-21206) and donkey anti-goat AlexaFluor 555 (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2023Quote: ... treated with a 10% fetal bovine serum (FBS)/PBS blocking solution (Fisher bioreagents, catalog #BP9703-100) and stained for eIF5A1/2 polyclonal antibody (Thermo Fisher Sci. catalog #PA529204, 1:200) or SMAD3 (Cell Signaling Technology catalog #9523S ...
-
bioRxiv - Bioengineering 2023Quote: ... cells were washed three times at 5 minutes each with DPBS before incubation for 1 hour at room temperature in the dark with secondary antibodies in blocking buffer (goat anti-rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody conjugated to Alexa Fluor 488 (ThermoFisher, 1:200 dilution, Cat # A-11008) or goat anti-mouse IgG1 Cross-Adsorbed Secondary Antibody conjugated to Alexa Fluor 488 (ThermoFisher ...
-
bioRxiv - Cell Biology 2023Quote: ... sections were washed two times for 8 min in DPBS containing 0.3% Triton X100 and then incubated for 1 hr in blocking solution containing the secondary antibody (1/200, Invitrogen P10994 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody ...
-
bioRxiv - Developmental Biology 2021Quote: ... peptide samples were purified via Stage tips using punched out cookies of C18 3M™ Empore™ extraction disc material (Fisher Scientific #14-386-2) as matrix ...