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Citations for Thermo Fisher :
4651 - 4700 of 5144 citations for HCCS Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 RBD WT for SPR binding assays (with N-terminal signal peptide and C-terminal thrombin cleavage site-TwinStrep-8xHis-tag) was expressed in Expi293F (Thermo Fisher Scientific) cells at 37°C and 8% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Eluted peptides were analyzed with an Orbitrap Fusion mass spectrometer coupled with an Easy-nLC 1000 (Thermo Fisher Scientific, Waltham, MA, USA). Mass spectrometer was operated in positive polarity mode with capillary temperature of 320 °C ...
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bioRxiv - Cell Biology 2021Quote: One ug of each sample with 50 femtomole of heavy labeled Peptide Retention Time Calibrant (PRTC) mixture (Thermo Fisher Scientific, cat # 88321) was loaded onto a 30 cm fused silica picofrit (New Objective ...
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bioRxiv - Biochemistry 2021Quote: Digested and desalted peptides were analyzed on an Orbitrap Fusion Tribrid mass spectrometer coupled to an EASY-nLC 1200 system (Thermo Fisher Scientific). Tryptic peptides were separated at a flow rate of 300 nl/min using a 190 min gradient from 5% ACN ...
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bioRxiv - Immunology 2021Quote: ... NC_045512.2 with N-terminal signal peptide and C-terminal thrombin cleavage site-TwinStrep-8xHis-tag) were expressed in Expi293F (Thermo Fisher Scientific) cells at 37°C and 8% CO2 ...
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bioRxiv - Bioengineering 2021Quote: ... The amounts of peptides to be tagged were normalized and mixed with 41 μL of TMT reagent (TMT sixplex, Thermo Fisher Sci) freshly dissolved in acetonitrile (20 μg/μL ...
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bioRxiv - Microbiology 2020Quote: ... The peptide extracts are reduced in volume by vacuum centrifugation and a small portion of the extract is used for fluorometric peptide quantification (Thermo scientific Pierce). One microgram of sample based on the fluorometric peptide assay was loaded for each LC-MS analysis.
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bioRxiv - Immunology 2021Quote: ... Equivalent aliquots of the digested peptides were analysed by LC-MS/MS using an Easy-nLC 1200 coupled to Q Exactive HF mass spectrometer (Thermo Fisher Scientific). The peptides were loaded onto a 20 × 0.1 mm i.d ...
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bioRxiv - Cell Biology 2022Quote: ... the peptides were analyzed with a nanoAcquity UPLC system (Waters) coupled to an Orbitrap Q Exactive HF mass spectrometer (Thermo Fisher Scientific) employing the LC-MS/MS protocol in a data-independent acquisition mode ...
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bioRxiv - Microbiology 2022Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive HF, Thermo Fisher Scientific) using a 80 min gradient ...
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bioRxiv - Immunology 2019Quote: ... Acidified eluted peptides were analysed in the data-dependent mode on a Q Exactive HF mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) online coupled to a UItimate 3000 RSLC nano-HPLC (Dionex) ...
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bioRxiv - Pathology 2019Quote: ... of the peptides were analyzed on a Q-Exactive Orbitrap mass spectrometer coupled to an Easy NanoLC-1000 system (Thermo Fisher Scientific). Each sample was analyzed in triplicate ...
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bioRxiv - Genomics 2019Quote: We analyzed fractionated peptides on an Orbitrap Fusion mass spectrometer coupled with the Nanospray Flex ion sources and an UltiMate 3000 UPHLC (Thermo Fisher Scientific). For each run ...
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bioRxiv - Physiology 2019Quote: ... and of those from GnHCl fraction (10µL, corresponding to 5 µg peptides) were loaded onto a spectrometer (Q-Exactive Quadrupole-Orbitrap, Thermo Scientific, Loughborough, UK) on a one-hour gradient with an intersample 30-minute blank loaded.
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bioRxiv - Cell Biology 2020Quote: ... 1.5 μg peptides were resuspended in 1% formic acid diluted in water and loaded on a Q Exactive Orbitrap mass spectrometer (Thermo Fisher Scientific). Settings used were as previously described [22] ...
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bioRxiv - Plant Biology 2019Quote: Effector candidates without their signal peptide were cloned into the pEarleyGate100 (pEG100) vector for plant expression [78] using Gateway cloning (Thermo Fisher Scientific). Candidate effector genes were amplified from genomic DNA isolated from spores of B ...
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bioRxiv - Cancer Biology 2020Quote: Peptides were analyzed on a Q Exactive HF interfaced with an Ultimate™ 3000 RSLCnano System (Thermo Fisher Scientific, San Jose, CA). The dried peptides and the enriched phosphopeptides were loaded onto a nano-trap column (Acclaim PepMap100 Nano Trap Column ...
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bioRxiv - Physiology 2021Quote: ... The stained bands were excised and processed for in-gel tryptic digestion (Shevchenko et al., 2006) and the eluted peptides were then analyzed using a Thermo Orbitrap Fusion Lumos Tribrid (Thermo Scientific™) mass spectrometer in data-dependent acquisition mode ...
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bioRxiv - Microbiology 2020Quote: LC-MS/MS analysis of tryptic peptides was performed on an Ultimate 3000 RSLC nano instrument coupled to a QExactive HF mass spectrometer (both Thermo Fisher Scientific). Tryptic peptides were trapped for 4 min on an Acclaim Pep Map 100 column (2 cm × 75 μm ...
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bioRxiv - Microbiology 2021Quote: ... Fractionated peptides (6 fractions in triplicate, total 18 runs) were analyzed on Orbitrap Fusion Tribrid mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) interfaced with Easy-nLC 1000 nanoflow liquid chromatography system (Thermo Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... Peptides were loaded onto a trap column (PepMap C18, 5 mm × 300 μm ID, 5 μm particles, 100 Å, Thermo Fisher Scientific) at a flow rate of 25 μL min−1 using 0.1% TFA as mobile phase ...
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bioRxiv - Immunology 2020Quote: ... FP8-GGGC peptides were conjugated to Qβ-VLPs using the bifunctional cross-linker succinimidyl 6-[(β-maleimidopropionamido) hexanoate] (SMPH; Thermo Fisher Scientific) as described previously for the conjugation of amyloid-beta (Aβ ...
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bioRxiv - Cancer Biology 2019Quote: LC-MS/MS analysis of iTRAQ-labeled tryptic peptide fractions was performed using an LTQ-Orbitrap Velos (Thermo Fisher Scientific, Waltham, MA). Tryptic peptides were separated prior to analysis using a Dionex Ultimate 3000 RSL C nanosystem (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2019Quote: ... equivalent aliquots of the digested peptides were analyzed in triplicate with an Easy-nLC 1200 coupled to a Q Exactive HF mass spectrometer (Thermo Fisher Scientific). A 20 x 0.1 mm i.d ...
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bioRxiv - Physiology 2019Quote: ... Inclusion bodies of the antigen peptides were harvested from the transformant Escherichia coli BL21 Star (DE3) (Thermo Fisher Scientific, Waltham, MA, USA) (37) ...
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bioRxiv - Biochemistry 2021Quote: ... The peptides were subjected to ESI source followed by tandem mass spectrometry (MS/MS) coupled online to the UHPLC (Thermo Fisher Scientific). Intact peptides were detected in the Orbitrap at a resolution of 60,000 ...
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bioRxiv - Biochemistry 2021Quote: Dansyl-derivatized or unmodified peptides after tryptic or chymotryptic digest were analyzed via LC- MS/MS with an LTQ Orbitrap Elite (Thermo Fisher Scientific) as follows ...
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Disruption of the TCA cycle reveals an ATF4-dependent integration of redox and amino acid metabolismbioRxiv - Biochemistry 2021Quote: The tryptic peptides were analyzed on a Fusion Lumos mass spectrometer connected to an Ultimate Ultra3000 chromatography system (both Thermo Scientific, Germany) incorporating an autosampler ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides were separated by a PepMapTM RSLC C18 EasySpray column (2 µm, 100Å, 75 µm x 50 cm) (Thermo Fisher Scientific, UK), operating at 50°C ...
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bioRxiv - Immunology 2020Quote: ... peptide separation using acetonitrile gradient was run for 120 min and (ii) data was collected on an Orbitrap Fusion (Thermo Fisher Scientific) operated at 120,000 resolution using HCD (higher-energy collisional dissociation ...
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bioRxiv - Biochemistry 2021Quote: ... 2 μL of each sample were subjected to reverse phase liquid chromatography for peptide separation using an RSLCnano Ultimate 3000 system (Thermo Fisher Scientific). Therefore ...
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bioRxiv - Biochemistry 2021Quote: ... These peptide mixtures were desalted using Oasis HLB cartridges (Waters, Milford, MA) and dried by vacuum evaporation (Thermo Fisher Scientific, Bremen, Germany). The digested peptides (n=30 ...
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bioRxiv - Cell Biology 2020Quote: ... Titanium dioxide-enriched peptides were introduced via an EASY-Spray nano-electrospray ion source into an Orbitrap Elite mass spectrometer (Thermo Fisher Scientific). Other samples were injected into Q-exactive mass spectrometers (Thermo Fischer Scientific) ...
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The derlin Dfm1 promotes retrotranslocation of folded protein domains from the endoplasmic reticulumbioRxiv - Cell Biology 2020Quote: ... peptides were separated by nano-flow reversed-phase liquid chromatography coupled to Q Exactive Hybrid Quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific). Peptides were loaded on a C18 PepMap100 pre-column (inner diameter 300μm×5mm ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides were separated on reversed phase C18 columns (precolumn: Acclaim PepMap 100, 300 μm × 5 mm, 5μm, 100 Å (Thermo Fisher Scientific); separation column ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides were reconstituted in LC-MS injection buffer (3% acetonitrile, 0.1% formic acid) and quantified by Pierce Quantitative Fluorometric Peptide Assay (Thermo Scientific, Product #23290).
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bioRxiv - Evolutionary Biology 2020Quote: ... 3 μl of each sample were subjected to reverse phase liquid chromatography for peptide separation using an RSLCnano Ultimate 3000 system (Thermo Fisher Scientific). Therefore ...
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bioRxiv - Evolutionary Biology 2020Quote: ... peptides were loaded on an Acclaim PepMap 100 pre-column (100 μm × 2 cm, C18, 5 μm, 100 Å; Thermo Fisher Scientific) with 0.07% trifluoroacetic acid at a flow rate of 20 μL/min for 3 min ...
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bioRxiv - Microbiology 2021Quote: ... The resulting peptides were analysed by online nanoliquid chromatography coupled to tandem mass spectrometry (UltiMate 3000 and LTQ-Orbitrap Velos Pro (Thermo Fisher Scientific) for the CPSF1 ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were reconstituted in 0.5 M TEAB and processed following the instruction manual for the TMT kit (Thermo Fisher Scientific, Waltham, USA). The tryptic peptides were fractionated by high pH reverse-phase HPLC using a Thermo Betasil C18 column (5 μm particles ...
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bioRxiv - Microbiology 2020Quote: ... before peptide separation on a nanoscale PepMap 100 C18 nanoLC column (3 μm, 100 Å, 75 μm i.d. × 50 cm, Thermo Fisher Scientific) at a flow rate of 0.2 μL per min using a 120 min gradient comprising 100 min from 4% to 25% of solvent B and 20 min from 25% to 40% of solvent B (solvent A consisted in 0.1% formic acid in water ...
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bioRxiv - Molecular Biology 2021Quote: ... analysis of peptide fractions was carried out on an EASY-nLC 1000 coupled to an Orbitrap Eclipse Tribrid mass spectrometer (Thermo Fisher Scientific). For each fraction ...
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bioRxiv - Immunology 2020Quote: ... assays [17] were generated from a mixture containing 100 fmol of each heavy reference peptide and shotgun data-dependent acquisition (DDA) LC-MS/MS analysis on a Thermo Orbitrap Fusion Lumos platform (Thermo Fisher Scientific). The setup of the μRPLC-MS system was as described previously [18] ...
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bioRxiv - Immunology 2021Quote: ... Protein identification by liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis of peptides was performed using an LTQ Orbitrap Velos MS (Thermo Fisher Scientific) interfaced with a nanoAcquity LC system (Waters ...
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bioRxiv - Immunology 2020Quote: ... 1 µg of digested peptides were loaded into mass spectrometry system (Q Exactive Hybrid Quadrupole-Orbitrap MS manufactured by ThermoFisher Scientific, USA). For separation of peptides Hypersil gold C18 (100x2.1 mm ...
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bioRxiv - Immunology 2020Quote: ... One population was pulsed with 50 μM of a relevant peptide and labeled with a high concentration (2.5 μM) of carboxyfluorescein diacetate succinimidyl ester (CFSE) (Molecular Probes, Eugene, OR). The other population was unpulsed and labeled with a lower concentration (0.25 μM ...
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bioRxiv - Cell Biology 2021Quote: ... Eluted peptides were introduced directly via a nanospray ion source into a Q Exactive Plus hybrid quardrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific). The mass spectrometer was operated in standard data dependent mode ...
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bioRxiv - Cell Biology 2021Quote: ... The peptides were separated on an EASY Spray C18 column (50 cm x 75 cm, 2 µm particle size) (ThermoFisher Scientific, Toronto) using an EASY nLC-1200 ...
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bioRxiv - Biochemistry 2021Quote: SARS-CoV-2 RBD proteins for SPR binding assays (residues 328-531 of S protein from GenBank NC_045512.2 with N-terminal signal peptide and C-terminal thrombin cleavage site-TwinStrep-8xHis-tag) were expressed in Expi293F (Thermo Fisher Scientific) cells at 37°C and 8% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... Peptide mixture concentration was estimated by measuring absorbance at 280 nm with a NanoDrop 2000 spectrophotometer (Thermo Scientific, San Jose, CA, USA) and a standard curve made from MassPREP E ...