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Citations for Thermo Fisher :
4901 - 4950 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Isolation of cells was performed either by enzymatic digestion using 3 mg/ml collagenase type II (Gibco Life technologies, Vienna, Austria) for 6-8 hours or migration from explants (explants were removed after 7-10 days ...
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bioRxiv - Molecular Biology 2020Quote: ... mESCs were transfected with 10ug pCAG vectors encoding 2xFlag-HA-tagged BAP1 wild-type or BAP1 C91S using Lipofectamine 2000 (ThermoFisher Scientific), according to manufacturer’s instructions ...
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Characterization of a novel Fgf10CreERT2 knock-in mouse line targeting postnatal lung Fgf10 lineagesbioRxiv - Developmental Biology 2021Quote: ... Then using sharp blades to cut the lung into small pieces and digested with 0.5% collagenase Type IV in HBSS (Life Technologies, Invitrogen) for 45 minutes at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... Fluorescent signals of eGFP production in wild type and mutant strains were measured by flow cytometry (Attune NxT Flow Cytometer, Thermo Fisher) (53).
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bioRxiv - Neuroscience 2022Quote: Healthy wild type control (WT) iPSCs derived were obtained from Thermo-Fisher Scientific (WT, GIBCO human episomal iPSC line, cat # A18945), which were generated via reprogramming of cord blood derived CD34+ progenitors ...
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bioRxiv - Microbiology 2022Quote: ... cells were treated with compound at a final DMSO concentration of 2% prior to transfection with 100 ng of the wild-type or triple mutant (L50F E166A L167F) plasmid with Lipofectamine LTX (ThermoFisher#15338100) for 24h ...
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bioRxiv - Biochemistry 2022Quote: ... then placed in fresh ice-cold HBSS and cut into small pieces to be digested in prewarmed HBSS with 1 mg/ml Collagenase Type 2 (Life Technologies) at 37 °C for 5 min with gentle agitation ...
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bioRxiv - Cell Biology 2022Quote: ... differentiated ALI-cultures were apically washed with PBS and treated with collagenase type II (1 mg/mL; Gibco, Waltham, MA, USA), diluted in advanced DMEM/F12 ...
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bioRxiv - Genomics 2022Quote: ... The protein lysate from the wild-type HEK293T cell and HEK293T transfected with the hAPOBEC3G-Myc-T2A-eGFP vector using Lipofectamine2000 (ThermoFisher Scientific) were used as negative and positive controls ...
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bioRxiv - Microbiology 2022Quote: ... growth medium (200 µL, 24 h), bacterial cultures (200 µL, 24 h of growth)) and pre-cleaned type 1 glass vials (VOA; Thermo Scientific) were chilled on ice for 10 min ...
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bioRxiv - Physiology 2023Quote: ... Total liver cholesterol and triglycerides were determined with colorimetric assay kits (L-Type Triglyceride M – Fujifilm Wako, Infinity Cholesterol Liquid Stable Reagent – Thermo Fisher) and normalized to total protein content.
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bioRxiv - Microbiology 2022Quote: BHK-21 cells were transfected with 1 μg of pCAGGS plasmid encoding the NL09 wild-type or escape mutant HA proteins using Lipofectamine (Invitrogen, USA) and Plus reagent (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: BHK-21 cells in 96-well plate were transfected with 100 ng of pCAGGS NL09 HA (wild-type or escape mutants) using Lipofectamine 3000 (Invitrogen, USA). At 24 h post-transfection ...
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bioRxiv - Neuroscience 2022Quote: ... Young and old cells of the five cell types were sorted into 150 μL of NeuroBasal-A medium (Gibco, 10888-022) with penicillin-streptomycin- glutamine (Gibco ...
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bioRxiv - Neuroscience 2022Quote: A well characterized CRISPR/Cas9 edited ‘del’ iPSC line carrying a homozygous 25bp deletion in exon 2 of RIC3 along with the isogenic unedited wild-type iPSC line (Gibco #A18945) were used in this study ...
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bioRxiv - Biophysics 2022Quote: Jurkat-wild-type (WT) cells were washed twice with PBS and then stained with the calcium sensor dye Indo-1 AM (Invitrogen I1223), at 37 °C and 5 % CO2 for 30 mins as described (Lo et al. ...
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bioRxiv - Cell Biology 2022Quote: ... was obtained from the American Type Culture Collection (CRL-11965) and cultured in Dulbecco’s modified Eagle’s medium (Gibco 41966-029, USA) supplemented with 10% fetal calf serum (Eurobio ...
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bioRxiv - Plant Biology 2024Quote: ... indica-infected wild-type and YUC9ox seedlings were extracted as described above and quantified using a Nanodrop ND-1000® UV/Vis spectrophotometer (ThermoFisher). RNA quality was additionally checked on a Bioanalyzer 2100 (Agilent ...
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bioRxiv - Systems Biology 2024Quote: ... a mixture of 50:50 carboxylate-modified Sera-Mag SpeedBeads type A and B (Cytiva) were washed three times with ddH2O (Barstead GenPure, Thermo Scientific) on a magnetic rack and 1 mg of combined beads were added to the E.coli sample ...
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bioRxiv - Immunology 2024Quote: Lungs were perfused with PBS-EDTA (0.5 mM) and digested in RPMI-1640 medium containing 1 mg/mL collagenase type IV (Life Technologies/ Thermofisher) and 30 µg/mL DNase I (Roche) ...
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bioRxiv - Physiology 2024Quote: ... The blocks were then treated with 1 mg/ml collagenase type II in Leibovitz’s L-15 Medium (Thermo Fisher Scientific Inc.) at 37°C for 30-45 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... Blood free perfused lungs were digested in Phosphate Buffered Saline (Mg and Ca free) with Collagenase Type I (Gibco Cat# 17100017), DNase (Millipore Sigma Cat# D5025) ...
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bioRxiv - Genetics 2024Quote: Flag-tagged wild type and mutated human RBBP5 plasmid were transfected to HEK293 cells by lipofectamine 3000 according to manufacturer’s protocol (Invitrogen, CA, USA). Cells were lysed by RIPA buffer and 20μg of whole cell lysate were used to assess protein expression in Western blot ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were purchased from the American Type Culture Collection (ATCC HTB-161; Manassas, VA) and maintained in RPMI 1640 medium (Gibco, 11875) supplemented with 10% FBS and 0.01 mg/ml bovine insulin (MilliporeSigma ...
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bioRxiv - Immunology 2023Quote: ... Ge518 (wild-type) cells were electroporated with 112 pmol sgRNA and 2 μg TrueCut Cas9 Protein v2 (Thermo Fisher Scientific, A36498) using a 4D-Nucleofector (Lonza) ...
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bioRxiv - Genomics 2023Quote: ... total RNA from expanded HUDEP-2 edited and wild-type clones was collected using the mirVana RNA isolation kit (ThermoFisher Scientific) or RNeasy Mini Kit (Qiagen ...
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bioRxiv - Bioengineering 2024Quote: ... stably expressing activated KRAS (HPDE-KRAS) and wild-type HPDE (HPDE-WT) were cultured in RPMI-1640 medium (Gibco, ThermoFisher Scientific) supplemented with 1% Penicillin-Streptomycin (Gibco) ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from frozen whole or cortical renal tissue of wild-type and mutant mice using a homogenizer and the PureLink RNA Mini kit according to the manufacturer’s instructions (Thermo Fisher Scientific). Total RNA (1 μg ...
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bioRxiv - Biochemistry 2023Quote: Four hundred wild-type or UCP1-expressing C2C12 cells were seeded in 200 μl of DMEM [high glucose DMEM with pyruvate (Gibco, 12800), 3.7 g/L NaHCO3 ...
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bioRxiv - Genomics 2023Quote: Total RNA was extracted from frozen mammary tissue of wild type and mutant mice using a homogenizer and the PureLink RNA Mini kit according to the manufacturer’s instructions (Thermo Fisher Scientific). Total RNA (1 μg ...
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bioRxiv - Molecular Biology 2023Quote: ... The stable wild-type hSSB1-GFP and 102A/115A/102&115A hSSB1-GFP mutants were generated with Lipofectamine 3000 (Invitrogen, L3000001) transfection followed by 500 μg/ml hygromycin B (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... Two micrograms of RNA harvested from the wild-type and ΔRS strains were reverse transcribed to generate cDNA using the Superscript III kit (Thermo Fisher). VirF primers (Xerri & Payne ...
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bioRxiv - Immunology 2023Quote: ... cell lines were purchased from the American Type Culture Collection (ATCC, Manassas, VA) and cultured in Roswell Park Memorial Institute (RPMI 1640; ThermoFisher Scientific) and supplemented with 10% FBS ...
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bioRxiv - Microbiology 2023Quote: The human type II lung epithelial cell line A549 (ATCC CCL-185) was cultured in DMEM/F12 with GlutaMAX (Life Technologies) supplemented with 10% FBS (FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Relative expression levels for zebrafish vtg2 in F2 vtg2- mutant and wild type female liver were measured using SYBR GREEN qPCR Master Mix (SYBR Green Master Mix kit; Applied Biosystems) as indicated by the manufacturer in a total volume of 10 µl ...
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bioRxiv - Immunology 2023Quote: Spleen cells from CD45.1+ CD45.2- male and CD45.1- CD45.2+ female C57BL/6 wild type mice were harvested as described above and stained with Cell Trace Violet cell proliferation kit (Molecular Probes) in PBS at different concentration ...
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bioRxiv - Molecular Biology 2023Quote: Madin-Darby canine kidney (MDCK) type IIG cells were maintained in low glucose MEM (#41090093, Thermo Fisher Scientific, GibcoTM, Paisley, UK) supplemented with 1 % (vol/vol ...
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bioRxiv - Genomics 2022Quote: ... two-thirds of the cells were collected for single-cell sequencing and the remaining cells were re-plated on 6-well plates coated with 5 μg/cm2 Type I rat collagen (Gibco, A1048301). Two additional samples were collected on Days 11 and 21 for single-cell sequencing ...
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bioRxiv - Genomics 2023Quote: Total RNA was extracted from frozen mammary tissue of wild type and mutant mice using a homogenizer and the PureLink RNA Mini kit according to the manufacturer’s instructions (Thermo Fisher Scientific). Total RNA (1 μg ...
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bioRxiv - Cancer Biology 2023Quote: ... and proteins were precipitated onto a 50:50 mixture of Sera-Mag Speed Bead types A and B (Thermo Fisher Scientific) in 70% acetonitrile ...
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bioRxiv - Physiology 2023Quote: ... we performed five iterative MS/MS deep sequencing runs on a pooled sample of each tissue type using the AcquireX algorithm (Xcalibur Version 4.3, Thermo Fisher Scientific). Lipids from these MS/MS spectra were then automatically annotated using LipidSearch (Version 4.2 ...
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bioRxiv - Neuroscience 2023Quote: ... stem cell cultures were washed in sterile Dulbecco’s phosphate buffered saline (DPBS) and incubated at 37°C in a Collagenase type IV/Dispase solution (1 mg/mL each, Thermo Fisher) until colonies detached from the feeder layer (typically after 15’–20’) ...
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bioRxiv - Biophysics 2023Quote: 12-well cultures of Hep-2 cells at 70% confluence were transfected with 0.5 μg of wild-type or mutant UL34 or UL31-FLAG plasmid using Lipofectamine as described by the manufacturer (Gibco-BRL) and incubated at 37 °C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Assorted tissues from third instar larvae (L3 stage) of the wild type Canton-S strain were dissected during 2-5 min in PBS (Thermo Fisher) with or without 10 % glycerol after turning larvae inside out with the help of fine tip forceps ...
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bioRxiv - Cell Biology 2023Quote: Rat RFL-6 fibroblasts were purchased from the American Type Culture Collection (ATCC) and cultured in Ham’s F-12K medium (Thermo Fisher Scientific) supplemented with 20% fetal bovine serum and 1% pen-strep ...
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bioRxiv - Bioengineering 2023Quote: ... The dermal layers of the skin were separated using forceps and further subjected to incubation with type I collagenase (1 mg/ml Gibco, USA) for 2 hours at 37 degrees ...
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bioRxiv - Microbiology 2023Quote: ... cell line was obtained from the American Type Culture Collection (ATCC) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen, GIBCO, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2023Quote: Wild-type (E14) and knockout (Smarca5-/-) mouse embryonic stem cells (mESC) were cultured in serum/LIF conditions: DMEM (Gibco, 11995-040), 15% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2023Quote: HeLa wild-type or HeLa POLE4 KO cells were grown in 8-well Lab-Tek II chambered coverglass 30 (Thermo Scientific) and transfected 48 h prior to imaging with GFP-tagged PARP1 chromobody (Chromotek) ...
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bioRxiv - Bioengineering 2023Quote: HEK293 and HEK293T from the American Type Culture Collection (ATCC) were cultured in Dulbecco′s modified Eagle′s medium (DMEM; Life Technologies) with 10% FCS (Life Technologies ...