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Citations for Cell Signaling Technology :
3051 - 3100 of 3293 citations for IL 2 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000, 71G4B7, Cell Signaling; beta actin 1:5000 sc-4778 ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 spike protein-specific IgG was measured using SARS-CoV2 spike protein serological IgG ELISA kit (Cell Signaling Technology) per manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... and corresponding polyvinylidene difluoride (PVDF) membranes were incubated overnight at 4°C with various antibodies: rabbit anti-Bcl-2 (1:500; #3498, Cell Signaling), rabbit anti-Bax (1:500 ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were incubated overnight at 4°C on a rocker with primary antibodies (Supplemental Table 2) according to the manufacturer’s instructions and then developed with HRP-conjugated secondary antibodies (Cell Signaling Technology) for 1 hour at room temperature on an orbital shaker ...
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bioRxiv - Molecular Biology 2023Quote: ... The antibody incubation was carried out overnight on a rotor at 4ºC in 100 μL antibody solution (wash buffer with 0.1% digitonin and 2 mM EDTA) containing 1 μL of αH3K27me3 Ab (Cell Signaling C36B11). Samples were then washed in dig-wash buffer (wash buffer with 0.1% digitonin) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then permeabilized and blocked with 0.1% saponin/0.1% BSA in PBS before incubated with α-SARS-CoV-2 Nucleocapsid primary antibody (Cell Signaling Technology) at 1:1000 in permeabilization/blocking buffer overnight at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... 10 mg of lysate representing one biological replicate was incubated for 2 hours at 4 °C with anti-Kv11.1 antibody (Cell Signaling #12889) covalently coupled to protein A/G magnetic beads (Thermo Fisher) ...
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bioRxiv - Immunology 2023Quote: ... Cells were blocked with 2% BSA in PBS for 1 hour at room temperature before antibody staining with rabbit ⍺-Sec61b (Cell Signaling) and mouse ⍺-Tom20 (Santa Cruz ...
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bioRxiv - Neuroscience 2024Quote: ... every 8th section of the brain was processed for double-fluorescent immunostaining for Ku80 and one of the following: Microtubule-associated protein 2 (MAP2; 8707, Cell Signaling), doublecortin (ab18723 ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 2 h blocking with 15% milk powder (in TBST) and overnight incubation with rabbit anti-BIP (3183, Cell signaling, 1/1000 for retina ...
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bioRxiv - Microbiology 2024Quote: ... The following antibodies were used for viral protein analysis: SARS-CoV-2 anti-Spike and anti-N protein (cat #569996 and #33336, respectively, Cell Signaling) and anti-β-actin (Sigma – cat #a2228) ...
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bioRxiv - Cancer Biology 2021Quote: ... or of 35 mg (for mouse tumors) total proteins using the PTMScan® Phospho-Tyrosine Rabbit mAb (P-Tyr-1000) Kit (Cell Signaling Technology), according to manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... Phosphorylated and total proteins were identified by immunoblotting using the following primary antibodies: Phospho-S6 Ribosomal Protein (Ser240/244) Antibody #2215 and α-Tubulin (DM1A) Mouse mAb #3873 (Cell Signaling Technologies) at 1:1000 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... The anti (pan)14-3-3 rabbit polyclonal antibody (#8312) and anti α-tubulin mouse monoclonal antibody (clone DM1A, mAb #3873) were purchased from Cell Signaling Technology. Anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Cancer Biology 2019Quote: ... the membranes were incubated overnight at 4 °C with the primary antibodies NF-κB p65 Mouse mAb (Cell Signaling Technology, diluted 1:1000) or TBP Rabbit mAb (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2019Quote: ... For surface immunostaining of endogenous HA-Nrxn1α live mouse cortical Nrxn1αHA/HA KI neurons were incubated with rabbit anti-HA (1:100 dilution; Cell Signaling Technology, Cat #3724) diluted in conditioned neuronal culture medium for 20 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... 500 μL of supernatant were transferred to tubes containing 25 μL of prewashed magnetic α-Myc beads (Myc-tag [9B11] mouse mAb magnetic beads conjugated #5698; Cell Signaling Technology) and incubated at 4°C for 2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... a mouse monoclonal antibody against the Ser19 phosphorylated form of myosin II regulatory light chain (P-MyoRLC) from Cell Signaling Technology (Danvers, MA), a rabbit monoclonal antibody against a peptide from human septin 2 from Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies used were horse anti-mouse HRP or goat anti-rabbit HRP which were both diluted 1:1,000 in block (Cell Signaling Technology, Danvers, MA).
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bioRxiv - Plant Biology 2021Quote: ... membranes were incubated at room temperature for 1 h with plant monoclonal anti-actin primary antibodies (CusAb, 1:1000) and 1 h with anti-mouse IgG HRP-conjugated secondary antibodies (Cell Signaling, 1:3000). Membranes were washed and developed as described above.
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bioRxiv - Cell Biology 2021Quote: ... Washed membranes were incubated with HRP-conjugated anti-rabbit or anti-mouse secondary antibodies as indicated (1:2000, Cell Signaling Technology, Beverly, MA) in 10 ml of blocking buffer with gentle agitation for 1 h at room temperature ...
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bioRxiv - Genomics 2021Quote: ... Individual clones were grown and stained for dCas9KRAB with a Cas9 antibody (Mouse mAb IgG1 clone 7A9-3A3 Alexa Fluor 647 Conjugate, Cell Signaling Technologies, 48796) to assess protein expression ...
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bioRxiv - Microbiology 2021Quote: ... and sera from individual animals were tested by ELISA with KLH-conjugated peptide immobilized on plastic surface and anti-mouse or anti-rabbit horse radish peroxidase (HRP)-conjugated detector Ab (Cell Signaling Technology, USA). The animals with the highest Ab titer were selected for booster immunization ...
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bioRxiv - Molecular Biology 2021Quote: ... and incubated for one hour with horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG (Cell Signaling Technology; catalog #’s: 7076 and 7074). Membranes were then washed three times in TBST only (five minutes per wash) ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins of interest were detected with HRP-conjugated horse anti-mouse IgG antibody (1:5000, 7076S, RRID:AB_330924, Cell Signaling Technology, Danvers, MA, USA) or HRP-conjugated goat anti-rabbit IgG antibody (1:5000 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed three times for 5 min with PBS and incubated in anti-mouse IgG Alexa Fluor® 555 Conjugate (Cell signaling #4409) at a dilution of 1:500 for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... three times and incubated with horseradish peroxidase-conjugated secondary antibodies (Mouse, Cell Signaling, cat #7076S; Rabbit, Cell Signaling, cat #7074S; Rat, Cell Signaling, cat #7077S ...
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bioRxiv - Microbiology 2022Quote: Rabbit anti-α-tubulin was purchased from Sigma (cat# SAB3501072) and goat anti-rabbit IgG HRP-conjugated and goat anti-mouse IgG HRP-conjugated were purchased from Cell Signaling (cat# 7076S).
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bioRxiv - Cancer Biology 2022Quote: ... and horseradish peroxidase (HRP)-conjugated rabbit and mouse secondary antibodies for immunoblot and MitoTracker Red CMXRos for immunofluorescence purchased from Cell Signaling Technologies (USA). Mouse monoclonal primary antibodies against p53 (DO-1) ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was then washed with 1× TBST (3 × 10 min) and incubated with anti-mouse IgG horseradish-peroxidase secondary antibody conjugate (1:10000 dilution; Cell Signaling Technology, 7076S) in 1× TBST for 1 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... the goat anti-mouse (polyclonal 7076) and goat anti-rabbit (polyclonal 7074) peroxidase-conjugated secondary antibodies were from Cell Signaling (Beverly, MA. USA). DMEM complete medium ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the primary antibody binding was directed against eGFP using a solution containing a 1/4000 dilution of a mouse anti-GFP 4B10 antibody (Cell Signaling Technology, USA). For all VLPs ...
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bioRxiv - Developmental Biology 2022Quote: ... MilliporeSigma, Cat# V8012), Rabbit anti-HA antibody (1:1000, MilliporeSigma, Cat# H6908) and Mouse anti-Myc antibody (1:1000, Cell Signaling, Cat# 2276S) as primary antibody ...
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bioRxiv - Genetics 2019Quote: ... and the primary antibodies were recognized by Alexa Fluor® 594 Donkey anti-rabbit IgG (H+L) (ANT030, Antgene) or Alexa Fluor® 555 Anti-mouse IgG (H+L) (4409S, Cell Signaling Technology) and shaken at room temperature for 1.5 hrs in the dark ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were incubated for 1 hour with 1:2000 dilution of either horseradish peroxidase (HRP)-linked horse anti-mouse IgG (Cell Signaling Technologies #7076) or HRP-linked goat anti-rabbit IgG (Cell Signaling Technologies #7074 ...
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bioRxiv - Microbiology 2020Quote: ... incubated O/N in primary antibody (Rabbit Pan-ADPr 1:1000, Cell Signaling E6F6A; Rabbit GFP 1:1000, Chromotek PABG1-100; Mouse Tubulin 1:1000; Cell Signaling DM1A). Primary incubation was followed with HRP-conjugated secondary antibodies (Rabbit-HRP 1:10000 ...
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bioRxiv - Molecular Biology 2019Quote: ... UK with catalogue numbers ab52908 and ab76227 respectively and housekeeping mouse raised β-tubulin (51 kDa) primary antibody was procured from Cell Signaling Technologies, MA ...
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bioRxiv - Neuroscience 2019Quote: ... the membranes were washed and incubated with either secondary goat anti-rabbit IgG or goat anti-mouse IgG at 1:5,000 dilution (Cell Signaling Technology, Danvers, MA) at room temperature for 90 min ...
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bioRxiv - Cell Biology 2019Quote: ... Blotted nitrocellulose membranes were incubated with primary antibodies (mouse -p53, SC-126, Santa Cruz Biotechnology; rabbit -CTCF, Millipore 07-729; rabbit -GAPDH, cst-2118, Cell Signaling Technology) followed by detection with HRP conjugated secondary antibodies (Jackson Immunoresearch Laboratories) ...
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bioRxiv - Immunology 2021Quote: ... The cells were washed twice with PBS and horse-anti-mouse-IgG-conjugated to HRP was added (1:2000 in 1% milk in PBS, Cell Signaling Technology, S7076) at room temperature for 45 min ...
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bioRxiv - Cell Biology 2022Quote: ... 0.1% Tween-20 in 1x TBS) and then treated with primary antibodies (YAP/TAZ, anti-rabbit, Cell Signaling, 8418 and beta-Actin, anti-mouse, Cell Signaling, 3700) overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... for 2h then incubated with primary antibodies (1:5000) overnight: anti-HA antibody (rabbit polyclonal antibody; Sinobiological #11684-T62) and anti-β-actin antibody (mouse monoclonal antibody; Cell Signaling #3700). The membrane was washed with TBS – Tween 20 0.1% ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies diluted in 5% skim milk in TBST: horse anti-mouse IgG (1:20,000, Cell Signaling Technology, cat# 7076), donkey anti-sheep IgG (1:20,000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were detected using goat anti-mouse or anti-rabbit IgG (H+L) Alexa fluor 488 or 647 (Cell signaling Technologies/ Bioconcept) secondary antibodies diluted 1:500 in 1% BSA 0.1% Triton-X 100 in PBS for 3 hours in the dark at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... β-actin [8H10D10] primary antibodies and anti-rabbit-HRP or anti-mouse-HRP antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). IRF3 [A19717] were purchased from ABclonal (Woburn ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was washed by 1× TBST 3 times and incubated with a secondary anti-rabbit-HRP or anti-mouse antibody-HRP (Cell Signaling, Danvers, MA) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated overnight at 4°C in 0.2% BSA/TBS with the following antibodies: anti-Myc mouse IgG (Cell Signaling, cat. No. 2276) for mDGKε-Myc staining and anti-STIM1 rabbit IgG (Cell Signaling ...
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bioRxiv - Cell Biology 2024Quote: ... To confirm the specificity of staining cells were incubated with isotype mouse (7076P2) or rabbit (7074P2) IgG antibodies (Cell Signaling, Danvers, MA, USA) (Fig ...
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bioRxiv - Cancer Biology 2024Quote: ... Cat # 69295) at (1:1000), anti-Rab27B (E4V30, Cat # 17572), and α-Tubulin Mouse mAb (DM1A, Cat#3873) all purchased from Cell Signaling Technology. The anti-Rab27B (Cat # 13412-1-AP ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-otoferlin (Developmental Studies Hybridoma Bank, HCS-1) diluted at 1:100 and rabbit anti-cleaved caspase-3 (Cell Signaling Technology, 9661) diluted at 1:250 ...