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Citations for Cell Signaling Technology :
3001 - 3050 of 3293 citations for IL 2 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... They were then incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies [HRP-conjugated anti-mouse IgG (1:5000; Cell Signaling Technology; #7076) and HRP-conjugated anti-rabbit IgG (1:5000 ...
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bioRxiv - Bioengineering 2024Quote: ... 45 µL of cells suspension were incubated for 90 min at RT in a total volume of 55 µL with 10 nM BioGCSFR and 1:200 Myc-Tag (9B11) Mouse mAb (2276, Cell Signaling Technology). After one wash with 500 µL PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were rinsed three times for 10 minutes and then incubated with horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse antibodies (1:2,500, Cell Signaling, #7074, #7076) at room temperature for 1 hour ...
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bioRxiv - Bioengineering 2024Quote: ... Hydrogels were stained with primary antibodies (mouse anti-KRT7, 1:300, NSJ Bioreagents catalog #V2656; rabbit anti-Ki67, 1:400, Cell Signaling catalog #9129 ...
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bioRxiv - Physiology 2024Quote: ... which was blocked using Odyssey blocking buffer (Li-Cor, USA). Membranes were incubated with primary antibodies (GAPDH, Cell Signaling, Cat. 97166 [D4C6R], mouse mAb, 1:1000; (A-Tubulin, Cell Signaling, Cat ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was blocked with Blocking One (Nacalai Tesque, Inc.) for 90 min and then reacted overnight with mouse anti-Myc monoclonal antibody (9B11, Cell Signaling Technology) (1:1000) ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... cells were incubated in the blocking solution with the HRP linked anti-mouse or anti-rabbit secondary antibody (respectively #7076 or #7074; Cell Signaling Technology) at 1:10,000 dilution (1 hr at RT in the dark) ...
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bioRxiv - Bioengineering 2024Quote: ... membranes were washed three times with TBS-T and incubated with HRP-conjugated goat anti-mouse (1:1,000, Cell Signaling Technology #7056) or goat anti-rabbit (1:1,000 ...
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bioRxiv - Immunology 2024Quote: ... Cells were then incubated for 45 min with 3 μg/ml Myc-Tag (9B11) Mouse mAb AlexaFluor 488 conjugate antibody (Cell Signaling Technology) and Phalloidin-Alexa 647 (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse secondary antibodies were used to assess protein signal (Thermo Scientific, Cell Signaling Technologies). Chemiluminescence was measured using an Odyssey Fc Imaging System (Licor ...
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bioRxiv - Biochemistry 2020Quote: ... #2983), Histone H3 (1:10,000, #4499), MEK1/2 (1:1000, #8727) and LDH (1:5000, #2012;) were from Cell Signaling Technology, Inc ...
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bioRxiv - Cancer Biology 2021Quote: ... and incubated overnight at 4°C with the primary antibodies Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (4370S, Cell Signaling) at 1:2000 ...
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bioRxiv - Cancer Biology 2021Quote: ... Other commercially available primary antibodies used were: anti-Phospho-p44/42 MAPK (phosphorylated ERK1/2, Thr202/Tyr204, Cell Signaling Technology #4377), anti-p44/42 MAPK (ERK1/2 ...
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bioRxiv - Cell Biology 2020Quote: ... Rabbit antibodies for anti-myosin-light-chain-2 (Cat #3672) and anti-phospho-myosin-light-chain-2 (raised against phospho-Thr18/Ser19; Cat #3674) were purchased from Cell Signaling and used at 1:500 (Western blot) ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining sample was incubated o/n on a rotary shaker at 4 °C with 2 μg of the appropriate antibody (IgG, 12-370 Merck; pSTAT1, 7649 Cell Signaling). The next day 25 μl Dynabeads resuspended in CDB/c were added and incubated for at least 1 h at 4 °C on a rotary shaker ...
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bioRxiv - Cell Biology 2022Quote: ... then seeded in DMEM/F12 and 1% serum plus antibiotic-antimycotic solution 48 hrs post-transfection cells were blocked and treated with Ab against α5β1 (Novus, 2-52680) and αv (Cell Signaling 4711) at 10ug/ml for 30 minutes prior to cell surface stripping (0.2M acetic acid ...
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bioRxiv - Immunology 2021Quote: ... followed by washing and 2 hour incubation with goat-anti-rabbit-HRP antibody at 1:5000 (all from Cell Signaling Technology). Membranes were washed again and detected with SuperSignal West Pico Chemiluminescent substrate (Thermofisher) ...
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bioRxiv - Biophysics 2021Quote: ... and phospho-myosin (rabbit phospho-myosin light chain 2 (Thr18/Ser19) antibody (dilution 1:50 in PBS 0, 5% BSA, #3674, Cell Signaling) for 2 h at RT ...
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bioRxiv - Neuroscience 2021Quote: Pericytes viability was assessed using the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay according to the manufacturer’s procedure (Cell Signaling Technology). Cells were plated at the appropriate concentration in 96-well plates under different experimental conditions ...
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bioRxiv - Plant Biology 2021Quote: ... the membrane was incubated overnight with the primary phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cell Signaling Technology, #9101). Subsequently ...
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bioRxiv - Developmental Biology 2020Quote: ... blocked and incubated with an antibody recognizing Myosin Light Chain 2 phosphorylated at serine19 (1:200, rabbit; Cell signaling technology, #3671), or with the anti-tyrosinated tubulin antibody YL1/2 (1:50 ...
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bioRxiv - Cell Biology 2022Quote: ... 10-15 μg of proteins were separated by SDS-PAGE and immunoblotted using the following antibodies: p-ERK1/2 (9101, Cell Signaling), ERK1/2 (4695 ...
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bioRxiv - Cancer Biology 2022Quote: ... Pre-cleared lysates were incubated over night at 4°C with end-over-end rotation with 2 µg of anti-Notch1 (D1E11, Cell Signaling) or anti-Cbl-b (G1 ...
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bioRxiv - Cell Biology 2021Quote: ... the membrane was incubated with antibody that recognizes proteins IDH 1 (1:1000) and IDH 2 (1:1000) (Cell Signaling, MA) and α-Tubulin (Abcam ...
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bioRxiv - Genetics 2022Quote: ... Beads were then resuspended in 250 μl blocking solution and 2 μg antibody (RAD21, Abcam ab992; CTCF, Cell Signaling Technology 3418) was added ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were probed overnight at 4 °C with antibodies against the following epitopes: phospho(Ser217/221)-MEK1/2 (1:1000; 41G9; Cell Signaling), MEK1/2 (1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... Where stated, cells were treated with 2 μM ruxolitinib (INCB018424, Stratech, S1378-SEL) or 50 nM bortezomib (Cell Signaling Technology #2204) or 1000 units of IFNβ (pbl Assay Science ...
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bioRxiv - Genetics 2020Quote: ... and bound Myc bead (1/2 total amount) samples were analyzed by SDS-PAGE and immunoblotting with rabbit anti-Myc monoclonal antibody (1:2000; Cell Signaling) to detect Myc-tagged EXOSC5 and EXOSC2 ...
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bioRxiv - Bioengineering 2020Quote: ... 1 x 108 induced cells were stained with 1 µM of directly AF488-labeled SARS-CoV-2 RBD and 0.5 µM anti-HA AF647 (Cell Signaling Technology) antibody ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Anti-rabbit IgG (H+L)-F(ab’)2 Fragment-Alexa Fluor 555 Conjugate (cat. No. 4413, Cell Signaling Technology, Beverly, USA), CRLS1 pAb (cat ...
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bioRxiv - Pathology 2020Quote: ... for one hour at 25°C and incubated overnight with the rabbit anti-phospho-MEK1/2 (Ser217/221) antibody (Catalog #9154; Cell Signaling), the mouse anti-MEK1/2 antibody (Catalog #4694 ...
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bioRxiv - Microbiology 2020Quote: ... Total ERK1/2 and phospho-ERK1/2 were detected using p44/42 MAPK antibody (1:1000 in 5% BSA/TBS-T, #9102, Cell Signaling) and phospho-p44/42 MAPK (Thr202/Tyr204 ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit polyclonal anti-phospho-myosin light chain 2 (Thr18/Ser19) (for immunostainings on elastomers; 1:200, Cell Signaling; cat. no. #3674). An anti-Plexin-B1 antibody was raised against the extracellular domain of Plexin-B1 ...
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bioRxiv - Physiology 2021Quote: ... were subjected to western blot analysis with antibodies against c-Jun NH(2)-terminal kinase (JNK) and phospho-JNK (Cell Signaling). Immunocomplexes were detected using the Licor Odyssey secondary detection system.
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bioRxiv - Cancer Biology 2021Quote: ... the degree of staining of the tissue specimens was observed under the Zeiss Apotome.2 microscope (20X objective, 0.8 NA) after DAB staining (Cell Signaling Technology) and hematoxylin (IHC World ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were stained with rabbit antibodies against phospho (S473)-AKT (p-AKT) and phospho (Thr202/Tyr204) p44/42 ERK1/2 (p-ERK) (4060, 9101; Cell Signaling) for 30 min ...
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bioRxiv - Cancer Biology 2021Quote: ... the slides were blocked with 3% BSA and incubated with primary antibodies against pERK1/2 (1:500, Cell Signaling Technology, 4370) or cleaved Caspase-3 (1:400 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and rabbit antibodies specific for p-Erk1/2 (phospho-p44/42 MAPK) (1:1000, #4370; Cell Signaling Technology, Beverly, MA, USA), Erk1/2 (p44/42 MAPK ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubated with the primary antibodies: polyclonal rabbit antibody against PTP1B (2 μg/mL; #5311; Cell Signaling Technology, Boston, MA, USA) and monoclonal mouse antibody against Cdk3 (2 μg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... containing 5% BSA for one hour at RT and incubated overnight at 4°C with anti-flotillin-2 (C42A3) (1:500, #3436, Cell Signaling), anti-calnexin (1:500 ...
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bioRxiv - Plant Biology 2022Quote: ... Phosphorylated MAPK proteins were detected by immunoblot analysis with antiphospho-p44/42 MAPK (Erk1/2)(Thr202/Tyr204) (D13.14.4E) rabbit mAb (Cell Signaling Technology, USA). The blotted membrane was stained with Coomassie Brilliant blue (CBB ...
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bioRxiv - Microbiology 2022Quote: ... After washing 3 times cells were blocked with 2% BSA for 1h at 37 followed by incubation with 1/100 dilution of anti-Flag monoclonal antibody (Cell Signaling) for 1.5h at RT ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was blocked with 5% non-fat dry milk in TBST and incubated with primary antibodies against Perilipin-2 (1:5000 gift from Dr. Ronal Kuhnlein) 13 and Actin (1:1000, Cell Signaling). The western blot images were captured by a BIO-RAD workstation.
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were incubated overnight at 4°C on a rocker with primary antibodies (Supplemental Table 2) according to the manufacturer’s instructions and then developed with HRP-conjugated secondary antibodies (Cell Signaling Technology) for 1 hour at room temperature on an orbital shaker ...
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bioRxiv - Immunology 2023Quote: ... Cells were blocked with 2% BSA in PBS for 1 hour at room temperature before antibody staining with rabbit ⍺-Sec61b (Cell Signaling) and mouse ⍺-Tom20 (Santa Cruz ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit monoclonal anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (1:2000, Cell Signaling Technology, Danvers, MA, USA,; RRID: AB_2315112), rabbit polyclonal anti-human SOD1 (1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... They were passed through a 0.45 µm filter and precleared with 2 μg/ml anti-rabbit isotype IgG antibody (Cell Signaling #2729) with protein A/G magnetic beads (Pierce ...
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bioRxiv - Biochemistry 2024Quote: ... 10 mg of lysate representing one biological replicate was incubated for 2 hours at 4 °C with anti-Kv11.1 antibody (Cell Signaling #12889) covalently coupled to protein A/G magnetic beads (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... Double resistant cell lines with dual integration of 4EBP1/2 shRNAs were validated by western blot (Cell Signaling Technology #9452, #2845) for efficient stable knockdown of the targeted proteins.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Immunolabeling was realized using the rabbit monoclonal primary antibody anti-phospho-ERK1/2 Thr202/Tyr204 (#4370, Cell Signaling Technologies, 1:2,000), or anti-total ERK1/2 (#05-1152 ...