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Citations for Cell Signaling Technology :
2851 - 2900 of 3293 citations for IL 2 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... following incubation with the primary antibodies anti-p53 (1:500, mouse, (Ab-6) Pantropic OP43 Sigma-Aldrich) and anti-GAPDH (1:1000, rabbit, 14C10 Cell Signaling) for 1h at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies used in this study were 1:2,500-diluted mouse anti-β-actin (Wako, #017-24551) and 1:1,000-diluted rabbit anti-cleaved caspase-3 (Cell Signaling, #9661). The membrane was washed three times with PBS for 10 minutes and incubated with secondary antibodies at room temperature for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... Tissues were then stained with the following fluorophore-conjugated anti-mouse antibodies for (1:100, 1.5 hr, room temperature, gentle agitation) in blocking buffer: Iba-1 (78060S, Cell Signaling Technology), F4/80 (123122 ...
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bioRxiv - Physiology 2023Quote: ... Membranes were washed three times with TBS-T for 5 min and incubated with peroxidase-linked anti mouse or rabbit IgG at a 1:10,000 dilution in TBS-T (#7076/#7074S, Cell Signaling, Germany) for one hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Respective expression was assessed by immunostaining using a mouse anti-HA antibody (1:500; Cell Signaling#2367, RRID AB_10691311 and a rabbit anti-Flag (1:500; Cell Signaling#14793 RRID AB_2572291 followed by Cy5-conjugated donkey anti-mouse (1.9 µg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... and then overnight at 4°C with the primary antibody (mouse anti-β-ACTIN, 1:30,000, Sigma; rabbit anti-AKT1, 1:1000, Cell Signaling; rabbit anti-AKT2 ...
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bioRxiv - Biochemistry 2022Quote: ... Blots were then washed three times with wash buffer and incubated 75 min at room temperature with a 1:10,000 dilution of goat anti-mouse HRP conjugated antibody (Cell Signaling Technology). After three washes with wash buffer ...
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bioRxiv - Microbiology 2023Quote: ... Anti-mouse IgG-horseradish peroxidase (HRP) (NA931; Cytiva, Tokyo, Japan) and anti-rabbit IgG-HRP (#7074; Cell Signaling Technology, MA, USA) were used as the secondary antibodies.
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bioRxiv - Molecular Biology 2023Quote: The following antibodies were used in this study: mouse monoclonal anti-β-actin (Cell Signaling Technology; 3700; RRID: AB_2242334; LOT# 20), rabbit polyclonal anti-SARS-CoV-2 nsp2 (GeneTex ...
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bioRxiv - Neuroscience 2023Quote: ... cells were resuspended in 250 μL1% BSA in 1X PBS containing Myc-tag Mouse monoclonal antibody (Cell Signaling, 9B11, Cat.#2276) with a ratio of 1:500 ...
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bioRxiv - Physiology 2023Quote: ... Protein expression was measured by standard western blot procedures using anti-mouse primary antibodies against Arf6 (rabbit monoclonal; 1:1000; 19kDa; Cell Signaling), β-actin (mouse monoclonal ...
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bioRxiv - Neuroscience 2023Quote: ... Trem2 was immunoprecipitated with either a Rabbit monoclonal antibody raised against the carboxy-terminus of mouse Trem2 (Cell Signaling Technology, 76765,) referred to as αTrem2-CT ...
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bioRxiv - Cell Biology 2023Quote: ... 1:2,000 for IB) and mouse anti-Myc-tag (cat. #2276, RRID: AB_331783; 1:500 for IB) were purchased from Cell Signaling Technology. Rabbit anti-calnexin (cat ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibody staining employed anti-mouse IgG and anti-rabbit IgG HRP-linked antibodies (Cell Signaling Technologies, #7076 and #7074, respectively) were used at 1:5000 dilution ...
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bioRxiv - Immunology 2023Quote: ... stimulated T cells were fixed with the Foxp3/TF staining buffer set (eBioscience) for 30 min at 4°C and stained intranuclearly with rabbit polyclonal anti-mouse HIF-1α (Cell Signaling) o/n in permeabilization buffer (eBioscience) ...
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bioRxiv - Molecular Biology 2023Quote: Mouse anti-MYC (2276), rabbit anti-HA (3724) and rabbit anti-FAM134B (83414S, for immunoprecipitation) were purchased from Cell Signaling Technology. Rabbit anti-FLAG (F3165) ...
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bioRxiv - Microbiology 2023Quote: ... Protein accumulation in the purified fractions was confirmed with western blot using anti-MBP tag (8G1) mouse monoclonal antibody (Cell Signaling) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... Secondary antibody was either goat anti-rabbit (7074) or horse anti-mouse (7076) IgG conjugated with horseradish peroxidase (Cell Signaling Technology). Staining was developed with SuperSignal West Dura Extended Duration Substrate (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies were probed with 1:1000 Alexa Fluor 488 conjugated anti-mouse IgG (Cell Signaling Technology, cat. No. 4408S), Alexa Fluor 594 conjugated anti-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies were probed with 1:1000 Alexa Fluor 488 conjugated anti-mouse IgG (Cell Signaling Technology, cat. No. 4408S), Alexa Fluor 594 conjugated anti-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2022Quote: ... then incubated in HRP-linked anti-mouse or anti-rabbit IgG antibody for 1h at 24°C with gentle rocking (1:5,000 dilution, Cell Signaling Technology). Membranes were washed in TBST and protein bands were visualized using the Pierce ECL detection system (Cat #32106 ...
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bioRxiv - Cancer Biology 2023Quote: ... and then incubated with horse radish peroxidase-linked anti-mouse or anti-rabbit secondary immunoglobulin antibodies (1:10000; Cat# 7076S, Cat# 7074S Cell signaling) in PBST for 40 mins ...
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bioRxiv - Cell Biology 2023Quote: ... to the following proteins were used for the immunodetection of proteins using horseradish peroxidase-conjugated anti-mouse (Cytiva, NA931) and anti-rabbit (Cytiva, NA934) secondary antibodies (1:1,000): CHOP (Cell Signaling, 5554), Cleaved Caspase-3 (Cell Signaling ...
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bioRxiv - Biochemistry 2023Quote: ... Primary antibodies were detected with an HRP-conjugated anti-mouse immunoglobulin-G at 1:5000 dilution (Cell Signaling Technology, Catalog# 7076S) for ZIP4 or an HRP-conjugated anti-rabbit immunoglobulin-G for β-actin at 1:5000 dilution (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed in PBS and incubated with fluorescent secondary antibody Goat anti-mouse Alexafluor 594 (8890, Cell signaling technology, USA). Membranes were mounted on slides with Fluoromount-G containing DAPI (00-4959-52 ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were then incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (1:5000, CS7076, Cell Signaling, Danvers, MA, USA) and goat anti-rabbit IgG (1:5000 ...
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bioRxiv - Cancer Biology 2023Quote: ... each membrane was probed with the appropriate horseradish peroxidase-conjugated secondary antibody (anti-mouse dilution 1:5,000; GTX213112-01, Gene Tex; anti-rabbit dilution 1:3000; 7074S, Cell Signaling Technology), and incubated for 1□h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were labeled with mouse anti-GFP (1:500) antibody and rabbit anti-FOXP1 antibody (Cell Signaling Technology; 1:400) and detected by biotinylated secondary antibody/streptavidin-Alexa Fluor 488 (GFP ...
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bioRxiv - Immunology 2024Quote: ... Primary antibody was removed by several washes with TBS + 0.1% Tween-20 and then subjected to incubation with secondary antibody (anti-mouse-HRP, 7076S, Cell Signaling Technology). The detection of protein bands was then carried out using enhanced chemiluminescence (ECL ...
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bioRxiv - Biochemistry 2023Quote: ... and incubated with a respective secondary HRP-conjugated antibody (anti-rabbit: Cell Signaling Technologies, #7074S, anti-mouse: Cell Signaling Technologies, #7076) for one hour at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Overexpression of Flag-NUDT15 and HA-NUDT18 were confirmed by analyzing cell lysates by SDS-PAGE followed by Western blotting using anti-FLAG-tag Rabbit antibody (#740001, Thermo Fischer Scientific) and anti-HA-tag (6E2) Mouse antibody (#2367, Cell Signaling) followed by anti-Rabbit HRP or anti-Mouse HRP ...
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bioRxiv - Microbiology 2023Quote: ... TMPRSS2 expression was evaluated using SimpleWestern Abby (ProteinSimple, San Jose, CA, USA) with an anti-Myc tag mouse monoclonal antibody (Cell Signaling Biotechnology ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed 5X with cold TTBS and incubated with anti-Rabbit or anti-mouse secondary antibodies conjugated to HRP (#7074 and #7076, Cell Signaling). After washing ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were blocked with 5% milk in TBST at room temperature for 60 min and probed with 1:1000 mouse-anti-E-cadherin antibody (#14472, Cell Signaling), 1:1000 rabbit-anti-Vimentin antibody (#5741 ...
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bioRxiv - Immunology 2024Quote: ... and stained with appropriate antibodies: anti-NF-κB p65 and Alexa488-conjugated anti-mouse lamin A/C antibodies (both from Cell Signaling) and DAPI (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies used were anti-rabbit and anti-mouse IgG HRP-linked antibodies (1:5000, Cell Signaling, 7074 and 7076 respectively). Images were developed using the MINI-MED 90 X-Ray Film Processor (AFP Manufacturing Corp) ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated with horseradish peroxidase-conjugated secondary IgGs (1:1,000 for rabbit #170-6515, mouse #170-6516, Bio-Rad, CA or 1:2,000 for #7076s, Cell signaling, MA), for 1hour at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... membranes were washed for 15 minutes in TBST and incubated with horseradish peroxidase-conjugated anti-mouse IgG (Cell Signaling; cat#: 7076) or IgM (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... which were detected with HRP-conjugated horse anti-mouse immunoglobulin-G antibody at 1:5000 dilution (Cell Signaling Technology, Cat# 7076) or goat anti-rabbit immunoglobulin-G antibody at 1:3000 dilution (Cell Signaling Technology ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were washed three times and incubated with anti-mouse IgG or anti-rabbit IgG HRP-linked antibodies (Cell Signaling Technology) both diluted in PBST (1:2,000) ...
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bioRxiv - Cancer Biology 2021Quote: ... permeabilised with 90% (v/v) methanol and then intracellularly stained with anti-phospho-p44/42 MAPK (Erk1/2; Cell Signaling) for 30 min at room temperature and then with secondary antibodies against rabbit IgG (H+L ...
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Ouabain enhances cell-cell adhesion mediated by β1-subunits of the Na+,K+-ATPase in CHO fibroblasts.bioRxiv - Cell Biology 2019Quote: The kinase inhibitors used were against cSrc (Dasatinib 100nM), AKT (Perifosine 10μM), ERK1/2 (U0126, 10μM) (0952, 14240 and 9903, respectively, Cell Signaling Technologies) and pNaKtide (1μM ...
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bioRxiv - Developmental Biology 2019Quote: ... and incubated overnight at 4°C with the rabbit anti-phospho-p44/42 MAPK (for pErk1/2) (Cell Signaling Technology) at 1:250 ...
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bioRxiv - Cancer Biology 2019Quote: ... Other primary antibodies used are anti–Phospho-p44/42 MAPK (phosphorylated ERK1/2, Thr202/Tyr204, 197G2, Cell Signaling Technology #4377), anti-p44/42 MAPK (ERK1/2 ...
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bioRxiv - Plant Biology 2021Quote: ... membranes were incubated overnight with rabbit polyclonal primary antibodies against phospho-p44/42 MAPK (Erk1/2) (Cell Signaling, 1:2000) at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... The following antibodies were used: rabbit anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (4370, 1:100, Cell Signaling), rabbit anti-Phospho-Drosophila p70 S6 Kinase (Thr398 ...
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bioRxiv - Physiology 2022Quote: ... anti-p44/42 MAPK (Erk1/2) (#4695) and anti-Acetyl-α-Tubulin (Lys40) (#5335) antibodies were from Cell Signaling Technology. Anti-Lamin A/C (sc-20681) ...
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bioRxiv - Cell Biology 2022Quote: ... The primary antibodies used for immunofluorescence staining were as follows: anti-Phospho Myosin Light Chain 2 (Cell Signaling Technology, #3671), anti-ZO-1 (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... and the plate was extensively washed with TBST + 100 mM EDTA and a 1:8,000 dilution of primary antibody (p-MEK1/2 (S217/S221) Rabbit Antibody (Cell Signaling)) was added to the plate and incubated for 1 hour ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies against the following proteins were used in the indicated dilutions: ERK1/2 (#4695, RRID:AB_390779, Cell Signaling Technology, 1:50), JUN (#9165 ...