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Citations for Cell Signaling Technology :
3251 - 3293 of 3293 citations for IL 2 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... and then incubated with the following primary antibodies for 2 hours at room temperature in 5% BSA: 1:1000 anti-SMARCC1 (Cell Signaling Technologies cat. #11956T), 1:1000 anti-SMARCA4 (Cell Signaling Technologies cat ...
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bioRxiv - Cell Biology 2023Quote: ... 10% FBS) and incubated with the following primary antibodies in blocking buffer for 2 hours at RT: YAP (Cell Signaling Technology, #14074, 1:100), TAZ (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The NC membrane was incubated for 1 hour at RT in blocking buffer (5% nonfat milk in PBS [pH 7.4] containing 0.1% Triton X-100 and 0.02% NaN3) and immunoblotted for 2 hours at RT with antibodies to CFTR (1:1000, Cell signaling Technology Cat. No. 78335,), SNAP-23 (1:1000 ...
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bioRxiv - Plant Biology 2024Quote: ... and MAPK activation was assessed by immunoblot analysis using primary anti-Phospho-p44/42 MAPK antibody (Erk1/I 2; Thr-202/Tyr-204) (Cell Signaling, Danvers, MA, USA) and secondary goat anti-rabbit-HRP antibody (Cell Signaling ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was incubated at 4°C overnight with the following primary antibodies: anti-phospho-MEK1/2 (Cell Signaling Technology, Inc., MA, USA, #9122), anti-MEK1/2 (Santa Cruz biotechnology ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was blocked with 2%BSA overnight and was probed with antibodies against goat anti-FLAG (1:1000, Cell Signaling Technologies, D6W5B, #14793), alpha Tubulin (1:10000 ...
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bioRxiv - Physiology 2020Quote: ... Membranes were washed and incubated with HRP-conjugated secondary antibodies at 1:10,000 (Mouse, Cell Signaling technology Cat# 7076, RRID AB_330924; Rabbit, Cell Signaling technology Cat# 7074, RRID AB_2099233) for one hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated overnight at 4 °C with the respective primary antibodies (rat-monoclonal anti- BrdU −1:200- Abcam; Rabbit polyclonal antibody anti-DCX,1:500- Cell Signaling; mouse monoclonal anti-NeuN- Millipore) diluted in a 0.1 % BSA and 0.125 % Triton X-100 solution ...
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bioRxiv - Microbiology 2022Quote: ... the cells were washed twice with the blocking buffer and incubated at 4 °C with primary antibodies (mouse anti-Strep, Qiagen, Cat #: 34850, 1: 150 dilution) (rabbit anti-PDI, Cell Signaling, Cat #: 3501, 1: 200 dilution) (rabbit anti-Flag ...
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bioRxiv - Microbiology 2022Quote: ... and then incubated at room temperature with HRP-conjugated secondary antibodies ((goat anti-rabbit HRP conjugated, Cell Signaling, Cat #: 7074, 1:5,000 dilution) (goat anti-mouse HRP conjugated, Cell Signaling, Cat #: 7076, 1:5,000 dilution) prepared in the WB blocking buffer ...
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bioRxiv - Neuroscience 2022Quote: ... NF-KB (1:1000 mouse anti-total NF-κB; Cell Signaling Technology #6956; RRID #AB_10828935 or 1:1000 rabbit anti-phospho NF-κB-S536; Cell Signaling Technology #3033; RRID #AB_331284); Akt (1:250 mouse anti-total Akt ...
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bioRxiv - Microbiology 2024Quote: ... blocked in 3% goat serum for 1 hour at room temperature and incubated in mouse anti-Sag1(28) [DG52] and anti-NF-κB (Cell Signaling Technology, 8242S, 1:1000 antibodies) overnight at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were then reacted with a secondary antibody (Anti-mouse IgG, and Anti-rabbit IgG conjugated to Horseradish Peroxidase (both from Cell signaling, Cat # 7076 and # 7074, respectively), developed using Western BrightTM enhanced chemiluminescence reagent (Advansta) ...
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bioRxiv - Microbiology 2023Quote: ... Wells were washed three times and incubated with HRP-labeled anti-mouse IgG H+L (1:2500 dilution in blocking buffer) (Cell Signaling Technology, Danvers, MA, USA, #7076P2). Plates were developed with 1-Step ultra TMB-ELISA substrate solution and incubated at room temperature for 15-30 min ...
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bioRxiv - Microbiology 2023Quote: ... Blots were washed for 30 min with TBS-0.05% Tween 20 (TBS-T) and primary antibodies were detected using horseradish peroxidase-conjugated goat anti-mouse secondary antibody diluted 1:2000 (Cell Signaling Technology, Inc., Danvers, MA, USA) and incubated at room temperature for 1 h ...
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bioRxiv - Cell Biology 2019Quote: The following primary and secondary antibodies were used for immunofluorescence: anti-phospho-Myosin Light Chain 2 (Thr18/Ser19) rabbit antibody (no. 3674, Cell Signaling Technology, 1:50 dilution); anti-E-cadherin rabbit antibody (no ...
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bioRxiv - Microbiology 2022Quote: ... Phosphorylation of MAPKs Slt2 and Fus3 was detected using a rabbit antiPhospho-p44/42 MAPK (Erk1/2) antibody (Thr202/Tyr204; #4370 from Cell Signaling Technology, Danvers, MA, USA). A mouse anti-α-tubulin antibody (#T9026 from Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... blocked with 5% nonfat dry milk for 1 hr and probed with primary Abs: phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204, dilution 1:2000, Cell Signaling Technology cat. no. 4370) and alpha tubulin (dilution 1:2000 ...
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bioRxiv - Cell Biology 2021Quote: ... 2% normal goat serum and 0.2% Triton-X100 in PBS) for 30 minutes and hybridized with polyclonal rabbit anti-phospho-Histone H3 (Ser10) antibody (Cell Signaling Technology, #9701; 1:1000) at overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... pERK1/2 (Thr202/Tyr204, #4370), α-pSTAT5 (Tyr694, #9351), α-Caspase3 (#9662), cleaved Caspase3 (Asp175, #9664), α-pMEK (Ser221, #2338) were purchased from Cell Signaling Technology. For RAF immunoprecipitation ...
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bioRxiv - Cancer Biology 2022Quote: ... and rotated at 4°C overnight with 2-5 ug of antibody (H3K27ac, Active Motif Cat #39133; V5 tag, ThermoFisher #R960-25; ETV6, Santa Cruz, # sc-166835x; ERG, Cell Signaling Technologies # 97249). Subsequent chromatin capture ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies were incubated for 2 h in a solution of TBST (0.05%) and LI-COR blocking buffer (5%) containing the following antibodies: anti-ERK1/2 (Cell Signaling Technologies; dilution factor: 1/2000) and streptavidin IRdye680 (LI-COR Biosciences ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting supernatants (10 μL) were analyzed by Western Blot with Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (Cell Signaling, 1679101S; dilution 1:1,000) primary antibodies ...
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bioRxiv - Plant Biology 2023Quote: ... MAPK activation was detected by western blot with Phospho-p44/42 MAPK (Erk1/2; Thr-202/Tyr-204) rabbit monoclonal antibodies #9101 (Cell Signaling Technology, Danvers, MA, USA). Blots were stained with PageBlue Protein Staining Solution (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... 1:1000) and TRIM46 (#1 in text: Abcam Cat# ab307967, RRID:AB_3096363, 1:200-1:1000, #2 in text: Cell Signaling Technology Cat# 92574, RRID:AB_3096364, 1:200), rabbit polyclonal antibodies against βIV-spectrin (M.N ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit monoclonal antibodies against TRIM46 (#1 in text: Abcam Cat# ab307967, RRID:AB_3096363, 1:500, #2 in text: Cell Signaling Technology Cat# 92574, RRID:AB_3096364, 1:1000), guinea pig monoclonal antibody against TRIM46 (Synaptic Systems Cat# 377 308 ...
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bioRxiv - Neuroscience 2019Quote: ... Samples were analyzed by western blot using a mouse anti-HA antibody (Covance, Cat #MMS-101P; Sorcs1HA/HA KI) or a rabbit anti-HA antibody (Cell Signaling Technology, Cat #3724; Nrxn1αHA/HA KI). A total protein stain (Ponceau ...
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Glutamine synthetase mRNA releases sRNA from its 3’UTR to regulate carbon/nitrogen metabolic balancebioRxiv - Microbiology 2022Quote: ... Then membranes were incubated for 1 h at RT with secondary anti-mouse or anti-rabbit HRP-linked antibodies (Cell Signaling Technology #7076 or # 7074; 1:5000) diluted in Bullet Blocking One and were washed three times for 15 min in 1×TBST buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were blotted on Nitrocellulose membrane (Cytiva) and revealed using following antibodies: mouse anti-AGR2 (Santa Cruz Biotechnology, Clone 6C5, 1/1000, rabbit anti-XBP1s (Cell Signaling Technologies, clone E9V3E, 1/1000), rabbit β-Tubulin-HRP (Abcam ...
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bioRxiv - Genetics 2023Quote: ... Alexa Fluor 488 anti-mouse IgG (H+L) (Catalog # 4408S) and Alexa Fluor 488 anti-rabbit IgG (H+L) from Cell Signaling Technology (1:200 dilution was used), CytoPainter phalloidin-iFluor 488 reagent (catalog # ab176753 ...
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bioRxiv - Biophysics 2023Quote: As primary antibodies we use mouse monoclonal anti-α-tubulin (dilution 1:250, Invitro-gen, #62204) and rabbit monoclonal anti-vimentin (D21H3) (dilution 1:200, Cell Signaling Technology, Danvers, MA, USA, #5741). As secondary antibodies we use goat anti-mouse Alexa Fluor 488 (dilution 1:500 ...
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bioRxiv - Physiology 2024Quote: ... or corresponding IgG controls (Mouse IgG2B Isotype Control MAB004 R&D Systems, Minneapolis, Minnesota, USA and rabbit IgG Isotype Control 3900S Cell Signaling Technology, Danvers, MA, USA). The following PLA steps ...
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bioRxiv - Neuroscience 2020Quote: ... we used identical procedures except that we used LV-Syn-DIO-mTagBFP2-2A-GluN2B (1 × 109 TU/ml titer, 2 μL) and GluN2B antibody (#4207S, Cell Signaling, 1:1000, rabbit polyclonal antibody) (Supplementary Fig ...
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bioRxiv - Immunology 2021Quote: ... anti-MEK1/2 mAb (Clone D1A5, Cat # 8727), and anti-phospho-MEK1/2 mAb (Ser221) (Clone 166F8, Cat # 2338) were purchased from Cell Signaling Technology (Danvers, MA 01923, USA). Human embryonic kidney 293 cells transformed with T antigen of SV40 (HEK293T ...
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bioRxiv - Biochemistry 2020Quote: ... Blots were washed 3 times with TBS-T (0.05% v/v Tween-20 in TBS) before incubation with HRP conjugated antibody (Cell Signaling Technology 7076S; anti-mouse-HRP or Cell Signaling Technology; 7074S; anti-rabbit-HRP) at 1:1000 in 2.5% (w/v ...
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bioRxiv - Biochemistry 2019Quote: ... Membranes were then incubated with the appropriate secondary antibodies in TBS-T for 30 min at RT at 1:4000 dilution (Cell Signaling Technology, Anti-rabbit #7074, Anti-mouse #7076). Following washes with TBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... blots were washed in 1x TBST and incubated with appropriate HRP-conjugated secondary (Goat anti-rat 7077, Cell Signaling, Goat anti-mouse 7076, Cell Signaling, Goat anti-rabbit 7074, Cell Signaling) for two hours with 2% milk powder in TBST ...
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bioRxiv - Zoology 2022Quote: ... The sections were then incubated with anti-phosphorylated-p44/42 mitogen-activated protein kinase (MAPK; extracellular regulated kinase, ERK1/2) rabbit monoclonal antibody (#4370, Cell Signaling Technology, Danvers, MA, USA; 1:500), which was used in zebrafish OE36 ...
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bioRxiv - Molecular Biology 2019Quote: ... Primary antibody incubation was carried out overnight in OBB 1:1 in PBS with the anti phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) rabbit monoclonal antibody (#4370 Cell Signaling technology. Used at 1:2000 dilution), the monoclonal anti GFP antibody ((0.4mg/ml) ...
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bioRxiv - Genomics 2023Quote: ... Fixed and permeabilized cells were then blocked in 0.5% BSA and 0.2% Tween-20 in PBS (IF blocking buffer) for 30 minutes at room temperature and incubated with anti-Ngn2 (Cell Signaling Technology, cat# 13144; 1:250 dilution) and anti-MAP2 (Biolegend ...
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bioRxiv - Cancer Biology 2022Quote: ... and probed overnight at 4°C with the following primary antibodies diluted in 5% BSA in TBST: pMEK1/2 (Cell Signaling Technology, Danvers, MA, USA, 41G9, 1:1000), pERK1/2 (Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2021Quote: ... Five-micron paraffin-embedded sections were labeled with primary antibodies against phosphor-p44/p42 MAPK (Erk1/2) (Thr 202/Tyr 204) (Cell Signaling Technology Cat# 4370, 1:200, Danvers, MA, USA), Ki-67 (ab15580 ...
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bioRxiv - Microbiology 2019Quote: ... 1:10,000 dilution]) and then with secondary antibody (horseradish peroxidase–conjugated anti-rabbit IgG [Cell Signaling Technologies, 7074S] or anti-mouse IgG [Cell Signaling Technologies, 7076S], both at a 1:5,000 dilution]).