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Citations for Cell Signaling Technology :
1351 - 1400 of 1408 citations for Western Blot Strip It Buffer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Biotinylated internalized proteins in lysates were captured by NeutrAvidin beads (Pierce) and eluted by incubating in Blue Loading Buffer (50 mM DTT, Cell Signaling, Danvers, MA) containing 50 mM DTT at 95°C for 5 min ...
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bioRxiv - Biochemistry 2022Quote: ... Then the cells were washed with cold phosphate-buffered saline (PBS) and lysed using RIPA buffer with protease inhibitor cocktail (#5871S, Cell Signaling, Danvers, MA) and phosphatase inhibitor cocktail (#78420 ...
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bioRxiv - Neuroscience 2024Quote: ... or 5% non-fat dry milk in TBST for 1 h then incubated overnight at 4°C in blocking buffer containing antibodies against TMEM106B (1:500, Cell Signaling Technology 93334), TDP-43 (1:1,500 ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked in 5% non-fat dry milk in TBST for 1 h and then incubated overnight at 4°C in blocking buffer containing the antibody against TMEM106B (1:500, Cell Signaling Technology 93334). The secondary antibody ...
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bioRxiv - Immunology 2024Quote: ... cells were fixed and permeabilized with the eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set (Thermo) for 30 minutes followed by staining with GATA6-PE (Cell Signaling Technologies) at 1:400 for 30 minutes on ice ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... The processed tissue was rinsed 3 times with PBS (20min each) to wash off the Dent’s Fix and then incubated with primary antibody in blocking buffer (pS6 Cell Signaling 2215, 1:1000) at 4°C with gentle rotation for 5 days ...
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bioRxiv - Immunology 2022Quote: ... Cells were stained for 1hr in permeabilization buffer with Myc antibody (Myc/n-Myc D3N8F Rabbit mAb PE Antibody, #35876, Cell Signaling, 1:50). Myc expression (expressed as geometric MFI ...
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bioRxiv - Immunology 2023Quote: ... At 4 hpi media was removed from the well by pipetting and the neutrophils were lysed in 30uL of 1X RIPA buffer (Cell Signaling Technology, 9806) containing cOmplete EDTA-free protease inhibitor cocktail (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then blocked with immunofluorescence (IF) block buffer (3% BSA in PBS) for 1 hour and incubated with primary antibodies: rabbit anti-γH2A.x (Cell Signaling 9718, 1:2000), rabbit anti-RPA32 (GeneTex GTX113004 1:2000) ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were then suspended in 50 µl Na-Phosphate buffer and incubated at room temperature for 10 minutes with desired antibody [20 µl of CTCF antibody (Cell Signaling Technology #2899) or 11 µg of anti-rabbit IgG (Thermo)] ...
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bioRxiv - Genetics 2023Quote: MEFs were washed twice with cold 1x PBS and lysed using RIPA buffer with a protease/phosphatase inhibitor cocktail (Cell Signaling Technology 5872) to obtain total protein samples ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were harvested at indicated time points and lysed in 1x cell lysis buffer (Cell Signaling Technologies, Inc, Danvers, MA, USA; Cat#9803) or 1× radioimmunoprecipitation assay (RIPA ...
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bioRxiv - Molecular Biology 2023Quote: Cells were washed with D-PBS (-) (Wako, 045-29795) and lysed using 140 µL of 10× Cell Lysis buffer (Cell Signaling Technology, 9803S) with PMSF (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then immunoblotted over night at 4°C with the indicated primary antibodies diluted in blocking buffer: Phosphorylated Stat1 (Tyr701) (D4A7) (Cell Signaling, 1:1000), STAT1 (Cell Signaling ...
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bioRxiv - Cell Biology 2023Quote: ... then with Duolink® In Situ Wash Buffer-A and counterstained with Alexa flour 488 conjugated E-cadherin antibody (3199S; Cell Signaling Technology) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated overnight at 4°C with the primary antibody diluted in blocking buffer or SignalStain® Antibody Diluent (cat. 8112, Cell Signaling Technology). Slides were incubated with biotinylated secondary antibodies for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: Adherent cells were washed in 1x PBS and lysed on the plate by the addition of complete RIPA buffer with protease (Cell Signaling Technology #5871S) and phosphatase (Cell Signaling Technologies #5870S ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue samples in Figures S5C and S5D were were obtained from TargetALS and homogenized in 6x tissue weight of 1x CST lysis buffer (Cell Signaling Technology, 9803) by TissueLyser ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were dried by lyophilization and resuspended in IAP buffer from PTMScan HS Ubiquitin/SUMO Remnant Motif (K-ε-GG) Kit (Cell Signaling #59322). K-GG containing heavy reference peptides were pooled and diluted into the IAP buffer resuspension fat a final concentration of 1 fmol per peptide ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were blocked in 4% FBS in 1x PBS + 0.1% Triton X-100 prior to incubation with primary antibodies diluted in blocking buffer: rabbit anti-γ-H2AX (Cell Signaling, 2577, 1:400); mouse anti-p53 (Cell Signaling ...
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bioRxiv - Developmental Biology 2019Quote: ... proteins were extracted from the heads of 30-50 wildtype and mdkami5001 embryos in cold RIPA lysis buffer containing protease and phosphatase inhibitor cocktail (Cell Signaling Tec., Danvers, MA). Proteins were separated in 12%Mini-PROTEIN TGX Precast gel (BioRad ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were then incubated for 2 hours at room temperature (RT) in antibody binding buffer (PBSTr +1% (v/v) DMSO) before overnight incubation with 1:1000 α-pSmad2 antibody (Cell Signaling Technology #18338) and ...
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bioRxiv - Microbiology 2019Quote: ... Pellets were washed once with ice-cold PBS and pelleted (as before) before resuspending pellets in 100 – 300 μl (depending on sample amount) RIPA buffer (Cell Signaling Technology, Danvers, MA) containing 1% CHAPS and 1% ASB-14 ...
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bioRxiv - Molecular Biology 2021Quote: Tissue was collected and incubated on ice for 60 minutes using RIPA buffer containing 1X RIPA including a phosphatase inhibitor Na3VO4 (Cell Signaling, Leiden, the Netherlands), 1 mM phenylmethylsulfonyl fluoride (PMFS ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and incubation with the primary antibodies in the blocking buffer for overnight at 4°C was followed by a two-hour incubation with secondary antibodies (Cell Signaling, Danvers, MA, USA) in the blocking buffer at room temperature ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... Membranes were then incubated with primary antibody diluted in TBS-T buffer (1:1000 Total OXPHOS Rodent WB Antibody Cocktail [abcam Cat# ab110413] or 1:1000 HSP60 XP Rabbit mAB [Cell Signaling Technology CAT#12165]) overnight at 4°C with gentle rocking ...
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Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: ... THP-1 macrophages were washed with 1X PBS and resuspended in cell extraction buffer supplemented with 1 mM PMSF and a protease inhibitor cocktail (Cell Signaling Technology (Danvers, MA). Nuclear and cytoplasmic protein fraction concentrations were quantified by the Bradford protein assay (Bio-Rad).
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were prepared in blocking buffer (SHIP1, 1:100 dilution, Cell Signaling Technologies; IBA1, 1:500 dilution, abcam; ASC, 1:500 dilution, Cell Signaling Technologies; P2RY12 ...
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bioRxiv - Cancer Biology 2024Quote: Total protein was extracted from spheroids and organoids using lysis buffer supplemented with a protease/phosphatase inhibitor cocktail (both from Cell Signaling Technology, MA, USA). Protein concentrations were measured using a Nanodrop 2000 system (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... we isolated by microdissection ovary stages 1-7 (see above) in 1X PBS and ground the material in RIPA buffer (Cell Signaling Technology, Danvers, MA), Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The membrane was washed four times in 0.1% TBST buffer and incubated for 1 h at room temperature with anti-rabbit IgG conjugated to HRP-linked antibody #7074 (Cell Signaling Technology, Danvers, MA) diluted 1:5,000 with 0.05% TBST containing 5% skimmed milk ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein was extracted with RIPA buffer and processed for acetylomic mass spectrometry as described previously55 using PTMScan® HS Acetyl-Lysine Motif (Ac-K) Kit (Cell Signaling Technology #46784).
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bioRxiv - Cancer Biology 2022Quote: The cells were harvested and lysed using radioimmunoprecipitation assay (RIPA) buffer supplemented with PMSF (1mM) and protease inhibitor cocktail (Cell Signaling Technology, Massachusetts, USA). The protein concentration was determined using Bradford protein assay (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... 10% FBS) and incubated with the following primary antibodies in blocking buffer for 2 hours at RT: YAP (Cell Signaling Technology, #14074, 1:100), TAZ (Sigma ...
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bioRxiv - Immunology 2023Quote: 18 µL U-87 MG EVs that had been separated by UC or SEC were lysed in 1x radioimmunoprecipitation assay buffer (RIPA, Cell Signaling Technology, catalog #9806) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Embryos were incubated for 1 hr in blocking buffer (PBS-Triton, FBS 10%) then overnight at 4°C with anti-Yap antibody (Cell Signaling, #4912, 1:200) in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... permeabilized with 0.5% Triton X-100 in blocking buffer for 15 min and incubated with primary antibodies against internal epitopes (DARPP32 (1:500, Cell Signaling Technology 2306, RRID:AB_823479) or PSD95 (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... Protein was extracted at desired timepoints by washing with ice-cold PBS and then lysing cells with 200 μL cell lysis buffer (Cell Signaling Cat. No. 9803) supplemented with protease inhibitor cocktail (Cell signalling Cat ...
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bioRxiv - Microbiology 2023Quote: ... peptide samples resuspended in IAP buffer were incubated with 100uL of antibody-bead conjugates specific for acetyl-lysine (Cell Signaling Technologies, PTMScan kits #13416). Immunoprecipitation proceeded overnight at 4°C with gentle mixing ...
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bioRxiv - Cancer Biology 2023Quote: Whole-cell extracts (WCE) of COV413A cells were prepared by scrape-harvesting 90%-95% confluent cells in 1X #9803 Cell lysis buffer (Cell Signaling Technology, Danvers, MA). Lysates were incubated at 4○C for 30 minutes prior to ultrasonic cavitation (3 x 15 seconds at 33% power ...
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bioRxiv - Immunology 2024Quote: ... Cells were subsequently washed with ice cold 1XPBS (Cellgro Technologies LLC) twice and then lysed with 150 μl 1X cell lysis buffer (Cell Signaling Technology, Danvers, MA), on ice for 10 min followed by removal of the lysed cells ...
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bioRxiv - Cancer Biology 2020Quote: Cells treated for 48 h with NaB were lysed with a lysis solution buffer and acetylation of H3 was measured with PathScan® Acetylated Histone H3 Sandwich enzyme-linked immunosorbent assay (ELISA) Kit (Cell Signaling Technology, Danvers, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... 20 µL of samples from each fraction were lysed with 5 µL 1:1 mixture of RIPA buffer (Cell Signaling Technology, Danvers, MA. Cat #: 9806S) and protease inhibitor (Santa Cruz Biotechnology ...
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bioRxiv - Systems Biology 2022Quote: ... Protein was solubilized in RIPA buffer and then subject to SDS-PAGE and immunoblotting with antibodies to total SRC and phospo-Y416 SRC (Cell Signaling, 2110 and 6943 respectively). Membranes were scanned using a Licor Odyssey fluorescent imaging instrument and densitometry conducted using Image Studio Lite ...
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bioRxiv - Cell Biology 2022Quote: ... After blocking the membranes were incubated cold overnight with primary antibodies diluted in Intercept (TBS) Blocking Buffer (E-cadherin mAb #14472 Cell Signaling Technology 1:1000, β-catenin mAb #8480 Cell Signaling Technology 1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were incubated overnight in primary antibody diluted in blocking buffer (1:1000 anti-cleaved caspase-3 Cell Signaling Technologies #9664, 1:4000 anti-α-Tubulin Sigma #T5168, 1:1000 anti-PARP Cell Signaling Technologies #9542 ...
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bioRxiv - Cancer Biology 2022Quote: ... the membranes were incubated overnight at 4°C with gentle agitation in primary antibody dilution buffer: phosphorylated ALK (Y1604) (Cell Signaling Technologies, #3341, 1:1000), ALK (Cell Signaling Technologies ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies were incubated for 2 h in a solution of TBST (0.05%) and LI-COR blocking buffer (5%) containing the following antibodies: anti-ERK1/2 (Cell Signaling Technologies; dilution factor: 1/2000) and streptavidin IRdye680 (LI-COR Biosciences ...
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bioRxiv - Physiology 2023Quote: ... Membranes were blocked with Odyssey blocking buffer (LICOR, Omaha, NE, USA) and incubated overnight with an antibody against hypoxia inducible factor-1α (HIF-1α, Cell Signaling Technology #36169; 1:500). Proteins were visualized using IRDye 800CW secondary antibodies (LICOR ...
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bioRxiv - Molecular Biology 2024Quote: ... approximately 20 mg of muscle from each biopsy specimen was placed in 1.7 mL tubes containing 400 μL of commercially available general cell lysis buffer (Cell Signaling; Danvers, MA, USA; cat#: 9803). Samples were centrifuged at 500 g for 5 minutes at 4ºC ...