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Citations for Cell Signaling Technology :
1201 - 1250 of 1336 citations for Western Blot Strip It Buffer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and the pellet containing the DOC-insoluble material was resuspended in 100 μl SDS solubilization buffer, pH8.8 (20 mM Tris-Cl, 2 mM EDTA, 1% w/v SDS, 1X protease inhibitor cocktail (Cell Signaling Technology, 5871). The DOC-insoluble pellet was thoroughly dissolved by heating the sample to 95 °C and vortexing ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight in blocking buffer at 4°C with the following primary antibodies: 1:2000 c-fos (Cell signaling technology), 1:2000 ds-red (Takara Bio) ...
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bioRxiv - Neuroscience 2019Quote: ... They were then incubated at 4°C overnight with the following primary antibodies diluted in buffer G: rabbit polyclonal anti-cleaved caspase-3 (Asp175) (Cell Signaling, #9661) at 1:250 ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei pellets were washed twice with 200 μL of Dig-wash buffer before resuspending in 100 μL Dig-wash buffer supplemented with 2 mM EDTA and a 1:50 dilution of H3K27ac primary antibody (Cell Signaling, 8173) and incubated overnight at 4°C on an overhead rotator ...
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bioRxiv - Genetics 2019Quote: ... The culture medium was then replaced with medium containing 50 mM biotin and cultured for a further 24 hours when the cells were lysed using RIPA Buffer (Cell Signaling Technology) with 1 µM phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Genetics 2020Quote: 293T cells seeded in six-well dishes and transiently transfected with expression plasmids were treated with 300 μls of RIPA lysis buffer (Cell Signaling Technology) and incubated on ice for 30 min ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were then washed with CyTOF permeabilization buffer and the barcoded samples were combined before being incubated with antibodies in a solution containing 10% NGS (Cell Signaling #5425). Cells were then washed with CyTOF staining buffer and fixed with 10% formalin (Merck #F5554 ...
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bioRxiv - Immunology 2021Quote: ... For phosphoflow experiments cells were immediately fixed by FoxP3/Transcription Factor Staining Buffer Set (eBioscience) for 10 minutes at 37°C after stimulation and labeled by pErk-AF488 (Cell Signaling, #13214) and pS6-APC (Cell Signaling ...
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bioRxiv - Cancer Biology 2020Quote: ... cells grown on chamber microscope slides were fixed in 4% paraformaldehyde and treated with immunofluorescence blocking buffer (catalog #12411, Cell Signaling Technology). Staining with primary antibodies against involucrin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated in a rabbit anti-HA monoclonal antibody (1:250 in blocking buffer; catalog no. 3724, Cell Signaling, Danvers, MA) for 48 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... 5 mg of tissue from distal ileal mucosal scrapings was lysed in 250 μL of ice-cold protein lysis buffer (Cell Signaling Technology Cell Lysis Buffer ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were incubated in TBXT buffer containing 5% non-fat milk and horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse IgG (Cell signaling tech.) and detected by using ECL Western Blotting Substrate (Pierce).
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bioRxiv - Cell Biology 2021Quote: ... the cells were fixed with 4 % paraformaldehyde and 0.2 % picric acid in phosphate buffer (pH 6.9) for 30 minutes at room temperature and incubated with peroxidase-conjugated anti-rabbit antibody (Cell Signaling technology #7074) overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... 2 μl cell lysates served as a source of the E3 ligase CHIP (cells were lysed in Cell lysis buffer (9803, Cell Signaling Technology). After reactions ...
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bioRxiv - Bioengineering 2022Quote: ... were washed with TBS buffer 3 times for 5 minutes and subjected to antigen retrieval using 1X SignalStain® Citrate Unmasking Solution (Cell signaling). The citrate buffer was bought to boiling temperature and added to the sections ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then incubated overnight at 4 °C with primary antibodies diluted in blocking buffer: anti-myosin-5a antibody (1:100, #3402, Cell Signaling Technology), or anti-myosin-6 antibody (1:200 ...
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bioRxiv - Cancer Biology 2022Quote: Flash frozen liver chunks were weighed and lysed with RIPA buffer (Astral Scientific, Australia) containing protease and phosphatase inhibitors cocktail (Cell Signaling Technology) at a concentration of 10 μL of RIPA per mg of tissue ...
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bioRxiv - Cell Biology 2022Quote: ... After blocking the membranes were incubated cold overnight with primary antibodies diluted in Intercept (TBS) Blocking Buffer (E-cadherin mAb #14472 Cell Signaling Technology 1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were lyzed by addition of lysis buffer with Protease Inhibitor Cocktail III (Bioprocessing Biochemical, California) and Phosphatase Inhibitor Cocktail (Cell Signaling Technology). Equal amount of protein (micro-BCA method ...
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bioRxiv - Neuroscience 2020Quote: ... Frozen brain lysates or hippocampi isolates were then homogenized with a T 10 basic ULTRA-TURRAX (IKa) in ice-cold buffer lysis (Cell Signaling 9803) and protease and phosphatase inhibitors (Roche) ...
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bioRxiv - Bioengineering 2021Quote: ... The fixed samples were permeabilized in PBS containing 0.1% Triton X-100 and 1% Fetal Bovine Serum for 30 min at room temperature before filling the channels with staining buffer (1.5% BSA in PBS) containing primary antibodies directed against cleaved caspase-3 (Cell Signaling Technology, 9661S) or VE-cadherin (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... 1 μL of each substrate solution was added to 1 mL of PBS or RIPA lysis buffer (Cell Signaling Technology, Danvers, MA) for NLuc experiments ...
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bioRxiv - Immunology 2022Quote: ... Cells were stained in 1X permeabilization buffer for 1 hour at room temperature with NFAT1-Alexa Fluor 488 (D43B1, Cell Signaling Technology). Nuclei were stained with DAPI prior to acquisition on an Amnis ImageStream X Mark II Imaging Flow Cytometer and analyzed with IDEAS software using the ‘‘Nuclear Localization’’ feature (EMD Millipore).
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bioRxiv - Immunology 2022Quote: ... Membranes were then washed and incubated in blocking buffer containing HRP-linked horse anti-mouse (1:5000; Cell Signaling Technology Cat#7076) for 45 minutes at room temperature ...
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bioRxiv - Pathology 2019Quote: ... kidney and heart tissue as well as Precision Cut Lung Slices (PCLS) and iHBECs were lysed in whole cell lysis buffer (Cell Signaling, UK) supplemented with protease and phosphatase inhibitors (Complete Mini protease inhibitor tablet and Phos-stop inhibitor tablets ...
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bioRxiv - Molecular Biology 2019Quote: ... The following primary antibodies diluted in the blocking buffer were used for staining: anti-eIF6 monoclonal antibody (D16E9) (1:300) (Cell Signaling Technology), or anti-eIF6 monoclonal antibody (1:350 ...
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bioRxiv - Genetics 2020Quote: ... Cells were briefly centrifuged to the bottom of the wells and incubated for 8 min (for VSMCs) or 15 min (for THP-1 cells) before lysis with cell lysis buffer (#9803, Cell Signaling Technologies) containing a cocktail of protease and phosphatase inhibitors ...
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bioRxiv - Cell Biology 2020Quote: Cells were trypsinized and washed with PBS and then lysed for 30 minutes on a rotating wheel at +4 °C in RIPA buffer supplemented with protease/phosphatase inhibitor cocktail (Cell Signaling, 5872). Lysates were sonicated with a Bioruptor Soni cation System (UCD200 ...
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked using Odyssey blocking buffer and incubated in primary antibodies overnight (αTubulin, 1:1000, Cell Signaling; SIRT3, 1:1000, Cell Signaling; MCAD 1:1000 ...
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bioRxiv - Biochemistry 2021Quote: ... The stimulation was stopped by mixing the platelet suspension with 10x concentrated ice-cold cell lysis buffer (1x f.c., Cell Signaling Technology #9803) containing Pefabloc SC-Protease-Inhibitor and phosphatase inhibitors (Carl Roth ...
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bioRxiv - Cell Biology 2019Quote: ... the cells were lysed in ODG buffer and the lysates were incubated with an anti-Myc-tag antibody (2276, Cell Signaling Technology). Immunoprecipitated proteins were pulled down with protein A-sepharose (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... Membranes were blocked for 1 hour at room temperature with blocking buffer (Licor) and incubated overnight at 4°C with anti-K48-linked poly-ubiquitin antibody (Cell Signaling Technologies) and anti-β-actin antibody (Santa Cruz Biotechnologies) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with ice-cold PBS and lysed in 100 µL of phosphatase and protease inhibitor containing RIPA buffer (Cell Signaling Technology). Cells were incubated on ice for 30 min and then harvested by scraping followed by centrifugation at 14,000 rpm for 10 min at 4 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were spun down and washed three times with cold Flow Wash Buffer (FWB) (PBS, 2% FBS, 2mM EDTA) and incubated with primary antibody (EGFR-D1D4J, Cell Signaling Technology) diluted in FWB for 60 minutes on ice ...
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bioRxiv - Biochemistry 2022Quote: ... Unroofed RBCs were blocked for 30 mins at room temperature in blocking buffer and then incubated with 1:200 rabbit anti-HA primary antibody (3724; Cell Signaling Technology) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... and 2.9 mg digests (for succinyl enrichment) were re-suspended in 1.4 mL of immunoaffinity purification buffer (Cell Signaling Technology, Danvers, MA) containing 50 mM 4-morpholinepropanesulfonic acid (MOPS)/sodium hydroxide ...
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bioRxiv - Immunology 2022Quote: ... and a secondary horseradish peroxidase-conjugated goat anti-rabbit antibody (1:1000 in blocking buffer for 1 hour, Cell Signaling Technology 7074S) with the peroxidase substrate KPL TrueBlue (30 minutes ...
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bioRxiv - Physiology 2023Quote: ... Beads were washed in lysis buffer and immunoblotting was done as described above using a PKA substrate monoclonal antibody (Cat#9624, Cell Signaling Technology).
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with primary antibodies diluted in blocking buffer over night at 4°C (SHIP1, 1:500 dilution, Cell Signaling Technologies; IBA1 ...
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bioRxiv - Physiology 2022Quote: ... The following day sections were washed in wash buffer 3 times for 5 min and co-stained for anti-Rabbit IgG Fab2 Alexa Fluor 488 (Cell Signaling, 4412S) at 1:1000 dilution at room temperature for 1 h ...
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bioRxiv - Molecular Biology 2022Quote: ... Dry peptides were then resuspended in an IAP buffer (Cell Signalling #59322) and incubated with anti-diGly antibody-conjugated slurry (Cell Signaling #59322) at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: hTERT RPE-1 Cdc7 wt or as cells or hTERT RPE-1 cells treated as above were harvested and lysed using RIPA buffer (1x, Cell Signaling Technology) supplemented with protease inhibitor cocktail (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed in TBST and then incubated for 45 min at room temperature in blocking buffer containing either anti-rabbit or anti-mouse HRP-linked secondary antibody (Cell Signaling Technology). Membranes were washed in TBST and imaged on the ChemiDoc Imaging System using SuperSignal chemiluminescent substrate (Thermo Scientific).
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bioRxiv - Immunology 2022Quote: ... Fly heads were cut off and placed into lysis buffer from PTMScanHS Ubiquitin/SUMO Remnant Motif Kit Protocol (#59322, Cell Signaling, UK), that contained 5% SDS in TEAB buffer (T7408 ...
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bioRxiv - Cancer Biology 2022Quote: EV and mixed samples were vortexed for 30s and incubated for 10min at room temperature in 1X RIPA buffer (Cell Signaling 9806S) and 1X Protease inhibitors (Santa Cruz sc-29131 ...
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bioRxiv - Physiology 2023Quote: Liver samples (~100 mg) were homogenized on ice for 60 seconds in 10X (v/w) RIPA Buffer (Cell Signaling, Danvers, MA, USA) with phosphatase and protease inhibitors (Boston BioProducts ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked using Intercept Blocking Buffer and incubated overnight in the following primary antibodies: rabbit GAPDH (2118S) from Cell Signaling Technology; rabbit BLOC1S1 (19687-1-AP ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells (2 x106/sample) were collected by centrifugation at 300 g for 4 min and resuspended in cell lysis buffer (Cell Signaling #9803) supplemented with protease inhibitors (Sigma #5056489001) ...
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bioRxiv - Cancer Biology 2023Quote: ... Digestion was stopped by adding 1× stop buffer (digitonin solution, RNase A, and 50 pg S. cerevisiae spike-in DNA (Cell Signaling Technology) for normalization ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were incubated with one of the following primary antibodies in antibody dilution buffer at 4°C overnight: anti-YAP1 (1:2,000 dilution; DBH1X; Cell Signaling, Danvers, MA), anti-CDX2 (1:100 dilution ...