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Citations for Cell Signaling Technology :
1151 - 1200 of 1336 citations for Western Blot Strip It Buffer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Primary and secondary antibodies were diluted in blocking buffer and used 1:100 for anti-FoxO1 (rabbit; Cell Signaling, 2880), 1:100 for anti-ESRRB (mouse ...
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bioRxiv - Cancer Biology 2021Quote: ... H460 shFTH1/pcDNA3 and H460 shFTH1/pcDNA3FTH1 cells were washed twice with cold PBS and incubated for 20 min with 300 μL of 1X Ripa Buffer (Cell Signaling) additioned with HaltTM Protease Inhibitor Single-Use Cocktail ...
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bioRxiv - Molecular Biology 2021Quote: ... The samples were diluted with lysis buffer without inhibitors and incubated overnight at 4°C with anti-PCNA antibody (1:500, #2586, Cell signaling) under mild agitation ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were briefly washed twice with ice-cold PBS supplemented with 1 mM activated sodium orthovanadate and then lysed using commercially available lysis buffer (Cell Signaling) supplemented with benzonase (ThermoFisher) ...
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bioRxiv - Molecular Biology 2022Quote: ... washed three times with TBST buffer and incubated over night with either primary antibody (rabbit ant-HA, #3724 and anti-GAPDH, #97166 antibody, Cell Signaling). The following day ...
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bioRxiv - Immunology 2022Quote: Cells were cultured in 8 well chamber slides and fixed in 4% paraformaldehyde and permeabilized using 0.1% saponin in blocking buffer (HBSS containing 3% BSA, 0.2% gelatin, and 0.02% NaN3) and stained with Rabbit anti-mouse YAP1 (Cell Signaling Technology, D8H1X), FITC-conjugated phalloidin ...
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bioRxiv - Immunology 2021Quote: The primary CD4+ T cells were lysed in the RIPA buffer with protease/phosphatase inhibitor cocktail (Cell Signaling Technology, Danvers, MA). After determining the protein concentration for each cell lysate ...
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bioRxiv - Immunology 2021Quote: ... Δagr and in-vitro-transcribed RNAIII (3 µg ml-1, 1 µg ml-1) were lysed in 30 µl of 1X cell lysis buffer (Cell Signaling) supplemented with 1X protease cocktail inhibitor (Roche ...
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bioRxiv - Molecular Biology 2019Quote: ... The transblot sheets were blocked with 5% normal donkey serum in TBST buffer Tris-buffered saline (10 mM Tris-HCl pH 7.5, 75 mM NaCl, #9997S, Cell Signaling Technology) and 0.1 % Tween 20] and incubated with diluted primary antibody for 1 hr at room temperature or overnight at 4°C ...
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bioRxiv - Immunology 2019Quote: ... Membranes were blocked for 1 h at RT in Odessy blocking buffer (Licor) and incubated overnight at 4°C with the following antibodies: IRF3 (Cell Signaling) 1:1000 ...
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bioRxiv - Molecular Biology 2019Quote: ... protein sample was transferred into a new microcentrifuge tube and the final volume was adjusted to 100 μL with general cell lysis buffer (Cell Signaling). Reduction solution (50 μL ...
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bioRxiv - Neuroscience 2020Quote: ... Blocking buffer was manually removed and replaced with either anti-Asymmetric Di-Methyl Arginine antibody (1:500, Cell Signaling Technology 13522) or anti-Symmetric Di-Methyl Arginine antibody (1:800 Cell Signaling Technology 13222 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were lysed on ice at 1000 cells/µL lysis buffer according to the manufacturer’s protocol of the active RAS detection kit (Cat.#8821; Cell Signaling Technology). Total protein concentrations of samples were measured using a BCA protein-assay kit (Cat.#23225 ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins samples were processed in SDS free lysis buffer for the assessment of Ras-GTP level using Active Ras Detection Kit (Cell Signaling) by following manufacturer’s instructions.
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bioRxiv - Developmental Biology 2022Quote: ... antigens were retrieved by boiling slides in citrate buffer (pH 6.0) for 1 min and pSMAD1/5 Ab (Cell Signaling, 41D10) was added overnight at 4°C ...
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bioRxiv - Biochemistry 2022Quote: Cell lysates for protein analysis were prepared by scraping cells in 1x Cell Lysis Buffer (Cell Signaling Technology, Danvers, MA, USA) containing 1x protease/phosphatase inhibitor (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: Human cancer tissue array staining was subjected to antigen retrieval at 98 °C for 20 min in EDTA buffer (pH 9.0) in a steamer and incubated with a rabbit anti-cleaved caspase 3 antibody (Cell Signaling, 9664L) 1:150 overnight at 4 °C followed by anti-rabbit 594 (Invitrogen A11012 ...
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bioRxiv - Cancer Biology 2022Quote: ... rinsed in phosphate buffer 0.2 % Triton for 15 min and incubated with rabbit anti-P-cad (1:50; Cell Signaling; 2130S) or anti-pSrc (pY419 ...
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bioRxiv - Cancer Biology 2022Quote: Cells with stable expression of FLAG-tagged human PDE6D were lysed in 1X cell lysis buffer (#9803, Cell Signaling, Danvers, MA) and incubated with anti-FLAG M2 Affinity Gel (A2220 ...
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bioRxiv - Molecular Biology 2020Quote: ... Briefly, sections were incubated with primary antibody (rabbit anit-PH3, 1:100 dilution in immunofluorescence buffer,) (Cell Signaling, Danvers, MA, 9701S) overnight at 4° C followed by goat anti-rabbit Alexa fluor 594 secondary antibody (1:400 ...
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bioRxiv - Microbiology 2020Quote: ... on ice for 15 min and washed once with 500 μl of nuclear extraction buffer B (Cell Signaling Technology, Cat. 7007), and then treated with 0.5 μL of micrococcal nuclease (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2022Quote: ... The remaining 4.9 mg of digests were re-suspended in 1.4 mL of immunoaffinity purification buffer (Cell Signaling Technology, Danvers, MA) containing 50 mM 4-morpholinepropanesulfonic acid (MOPS)/sodium hydroxide ...
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bioRxiv - Physiology 2022Quote: Liver samples (∼50 mg) were homogenized on ice for 60 seconds in 10X (v/w) RIPA Buffer (Cell Signaling, Danvers, MA) with protease and phosphatase inhibitors (Boston BioProducts ...
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bioRxiv - Cancer Biology 2019Quote: ... samples were resuspended in 750 uL 1X IAP Buffer (50 mM MOPS pH 7.2, 10 mM sodium phosphate, 50 mM NaCl from Cell Signaling Technology) using vortex and brief bath sonication ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were then centrifuged again and removed of supernatant before resuspension in isolation buffer (calcium and magnesium-free 1xPBS, 0.1% (w/v) bovine serum albumin (BSA) (Cell Signaling Technology), 2mM EDTA ...
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bioRxiv - Cell Biology 2021Quote: ... The protein A agarose bead was added with 450 µl of IP buffer and 5 µl of Myc antibody (Cell Signaling). The mixture was then incubated on rotator at 4°C for 4 h ...
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bioRxiv - Molecular Biology 2021Quote: ... ChIP was performed using an amount of BEL-ChIP nuclear extract corresponding to 3.4 μg of input genomic DNA in 250 μL of ChIP Buffer + 1X PIC (SimpleChIP Kit #9003, Cell Signaling Technology). Antibody was added according to the vendor’s specifications and incubated overnight with end-over-end mixing at 4°C ...
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bioRxiv - Immunology 2019Quote: Samples were washed twice in ice cold HBS and then lysed in 1000ml lysis buffer for 30 minutes at 4°C with end over end mixing (Cell Signaling Lysis Buffer 20mM Tris-HCL pH7.5 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... MCF7 cell lysate as prepared above was diluted to 0.8 mg/mL total protein in 1:3 lysis buffer:dilution buffer with 0.5 nM rabbit anti-BRD4 antibody (Cell Signaling Technology; E2A7X) and 1 nM CoraFluor-1-labeled anti-rabbit nano secondary ...
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bioRxiv - Neuroscience 2022Quote: ... 20 third instar larvae per genotype were flash frozen in liquid nitrogen and homogenized in 100 μL RIPA buffer (Cell Signaling) supplemented with cOmplete protease inhibitor cocktail (Roche ...
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bioRxiv - Cancer Biology 2022Quote: ... harvested at 50-80% confluence and lysed by sonication in RIPA buffer (Cell Signaling Technology; 20 mM Tris-HCl (pH 7.5), 150 mM NaCl ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were reblocked with PBS Blocking Buffer and reprobed overnight at 4°C with anti-Akt antibody (Cell Signaling Technology #9272) diluted 1:1,000 in PBS antibody buffer ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... membranes were incubated with the following primary antibodies in the blocking buffer: rabbit anti-GAPDH (1:2,500; Cell Signaling Technology #2118) and mouse anti-HiBiT (1.0 µg/ml ...
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bioRxiv - Physiology 2022Quote: ... Isolated islets and MIN-6 cells were mechanically homogenized in ice cold RIPA lysis buffer (Cell Signaling Technology, Danvers, MA, USA) supplemented with 1x protease inhibitors cocktail (Sigma ...
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bioRxiv - Microbiology 2023Quote: Samples used for measuring HMGB1 protein levels in the stool and colonic mucus were homogenized in cell lysis buffer (Cell Signaling) containing complete protease inhibitor (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies were diluted in blocking buffer and applied for 1 hour at RT: rabbit anti-p53 (Cell Signaling Technology, 2527) at 1:200 dilution ...
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bioRxiv - Immunology 2023Quote: ... stimulated T cells were fixed with the Foxp3/TF staining buffer set (eBioscience) for 30 min at 4°C and stained intranuclearly with rabbit polyclonal anti-mouse HIF-1α (Cell Signaling) o/n in permeabilization buffer (eBioscience) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The membranes were then incubated in the same buffer with anti-TAZ and anti-β -actin antibody (#8418, #5125, Cell Signaling) or anti-IHH antibody (#13388-1-AP ...
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bioRxiv - Molecular Biology 2023Quote: ... washed twice in PBS and after centrifugation at 250 x g for 5 min at 4°C resuspended in cell lysing buffer (Cell Signaling Cat# 9803 ...
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bioRxiv - Physiology 2023Quote: ... Tibialis anterior (TA) and EDL were homogenized using an MP homogenizer in RIPA buffer (#9806, Cell Signaling Technologies Inc., Danvers, MA) supplemented with a protease and phosphatase inhibitor cocktail (Halt ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then diluted in three volumes of flow cytometry buffer (1x PBS, 25 mM HEPES pH 7.4, 1% bovine serum albumin [Cell Signaling Technology #9998] ...
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bioRxiv - Cancer Biology 2023Quote: ... The DNA fragments were purified using the DNA purification buffers and spin columns (ChIP, CUT&RUN, Cell Signaling Technology, cat# 14209) and SYBR Green-based real-time PCR was conducted ...
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bioRxiv - Molecular Biology 2023Quote: ... The antibody incubation was carried out overnight on a rotor at 4ºC in 100 μL antibody solution (wash buffer with 0.1% digitonin and 2 mM EDTA) containing 1 μL of αH3K27me3 Ab (Cell Signaling C36B11). Samples were then washed in dig-wash buffer (wash buffer with 0.1% digitonin) ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was rapidly weighed and homogenized with a pestle homogenizer in the following solution: 1x Cell Lysis Buffer (Cell Signaling, 9803S), 0.5% (v/v ...
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bioRxiv - Immunology 2023Quote: ... Tissues were then stained with the following fluorophore-conjugated anti-mouse antibodies for (1:100, 1.5 hr, room temperature, gentle agitation) in blocking buffer: Iba-1 (78060S, Cell Signaling Technology), F4/80 (123122 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were lysed by ice-cold RIPA buffer (150mM NaCl, 1% NP40, 0.1% SDS and 50mM Tris, PH7.4) containing protease and phosphatase inhibitor (Cell Signaling Technologies; 5872) for 20 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... Chromatin was sheared using a Covaris Sonicator for 20 minutes in sonication buffer (SimpleChIP Plus Sonication Kit, Cell Signaling Technology, #57976) to obtain fragments in the range of 200-500 bp ...
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bioRxiv - Neuroscience 2024Quote: ... Staining with anti-Pu.1 antibody in 1X Permeabilization buffer (microglia marker 1:50, Pu.1-AlexaFluor 647, 9G7 Cell Signaling) was performed for 40 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to nitrocellulose membranes at 100 volts for 90 minutes in cold 1X transfer buffer (Cell Signaling Technology, 12539S). Membranes were blocked with 5% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... the pellet was washed with PBS (–) and incubated on ice for 5 min in 0.1 ml cell lysis buffer (Cell Signaling Technology, Inc.). In the other conditions ...