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Citations for Cell Signaling Technology :
1251 - 1300 of 1336 citations for Western Blot Strip It Buffer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... cells were harvested at indicated time points and lysed in 1× radioimmunoprecipitation assay (RIPA) buffer (Cell Signaling, Danvers, MA, USA; Cat# 501015489) containing 1× protease inhibitor cocktail (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolated Tregs were fixed and permeabilized using the Foxp3 Transcription Factor Fixation/Permeabilization buffer (eBioscience) and then incubated with unconjugated anti-FOXP3 (Cell Signaling Inc.) antibody and anti-SRC-3-Alexa647 (sc-5305 ...
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bioRxiv - Immunology 2023Quote: ... The cells were then suspended in 50 µl antibody buffer pre-mixed with an anti-Flag antibody (1:50; Catalog:14793, Cell signaling Technology) or with anti IgG control (Catalog:2729S ...
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bioRxiv - Immunology 2023Quote: ... washed twice with PBS then resuspended in cold NP-40 lysis buffer (1% NP-40, 50mM Tris-Cl, 150mM NaCl) with protease inhibitors (Cell Signaling, #5871) on ice for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... neurons and mouse brain tissues were lysed for 30 minutes on ice in appropriate volume of cold RIPA lysis buffer (Cell Signaling Technologies) supplemented with protease inhibitor cocktail ...
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bioRxiv - Cancer Biology 2023Quote: ... Beads were resuspended in 1ml total volume cold Co-IP Buffer containing rabbit IgG or rabbit anti-FLAG antibody (Cell Signaling #14793S), 4μg per sample ...
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bioRxiv - Bioengineering 2023Quote: ... Radioimmunoprecipitation assay buffer (RIPA) 10x and the ROCK inhibitor Y-27632 (cat# 13624S) were purchased from Cell Signaling Technologies (Whitby, ON, Canada). BrainPhys™ Neuronal Medium was obtained from STEMCELL Technologies (Vancouver ...
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bioRxiv - Immunology 2023Quote: ... The Ce3D™ Permeabilization/Blocking Buffer was discarded and replaced with Alexa Fluor 647 conjugated anti-mouse Iba-1 (1:100, 78060S, Cell Signaling Technology) in Ce3D™ Antibody Diluent Buffer (48 hr ...
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bioRxiv - Cancer Biology 2023Quote: ... and samples were prepared by boiling at 99 °C for 10 minutes with Blue Loading Buffer (7722S; Cell Signaling Technology, Danvers, MA). Then ...
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bioRxiv - Neuroscience 2024Quote: ... sheared chromatin was centrifuged at 16,000 x g for 20 minutes at 4 °C and dissolved in 1x ChIP buffer (Cell Signaling Technology, #57976). 2% input was taken and chromatin was incubated with antibodies overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: Retinas that were collected were homogenized on ice for 1 min using a modified RIPA lysis buffer (Cell Signaling Technology, MA, USA) as previously described2 ...
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bioRxiv - Biophysics 2023Quote: ... the blots were washed 3 times with TBST buffer and shaken gently with 5 mL primary antibody (rabbit anti-MET, #4560, Cell Signaling Technology, 1:1000 dilution ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant containing the enriched chromatin samples was saved and purified using DNA Purification Buffers and Spin Columns (#14209, Cell Signaling, USA). The recovered chromatin samples were then subjected to qPCR analysis using primer pairs specific to INK4A promoter region sequences (INK4A−1kb_FW ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary and secondary antibodies were diluted in FACS buffer and used 1:100 for anti-phospho-Akt(Ser473) (rabbit; Cell Signaling, 4058) and 1:500 for anti-rabbit Alexa-647 (goat ...
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bioRxiv - Genomics 2024Quote: ... Cell lysate was centrifuged at 21,000 x g for 10 minutes and supernatant was mixed with Blue Loading Buffer (Cell Signaling Technology #7722) per the manufacturer’s recommended ratio ...
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bioRxiv - Developmental Biology 2021Quote: About 3×108 cells stored in BD Phosflow™ Perm Buffer III were washed twice with 1% PBSA buffer (PBS containing 1% BSA) and then incubated with rabbit anti-SOX1 antibody (Cell Signaling Technology, #4194) at 1:150 dilution for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... Final cell pellets were lysed on ice for 10 min in 1X RIPA buffer (cat. no. 9806S, Cell Signaling Technology, Danvers, MA, USA) with added protease inhibitors (cat ...
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bioRxiv - Molecular Biology 2020Quote: Liver samples (∼100 mg) were homogenized on ice for 60 seconds (20 second increments) in 10X (v/w) Cell Signaling Lysis Buffer (Cell Signaling, Danvers, MA) with protease and phosphatase inhibitors (Boston BioProducts ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were further washed with PBS-T buffer and incubated with secondary antibodies (Cell Signaling Technology, #14708 or #14709, 1:10,000 dilution) for 2 hrs at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Fixed cells were stained with rabbit anti-mouse COX-2-Alexa488 at 1:100 dilution in perm wash buffer (Cell signaling, cat #13596S) for 1 hr at RT ...
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bioRxiv - Cancer Biology 2022Quote: Whole cell protein was extracted from cells treated for 24 h with 1 mM aspirin and 4 μM regorafenib using radio-immunoprecipitation assay buffer (Cell Signaling Cat# 9806) supplemented with 1 mM phenylmethanesulfonyl fluoride (Cell Signaling Cat# 8553) ...
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bioRxiv - Pathology 2022Quote: Cardiac proteins were extracted using freeze crusher (SK-100, Tokken Inc., Chiba, Japan) and cell lysis buffer (#9803, Cell Signaling Technology, MA, USA). Protein lysate was separated by SDS-PAGE and transferred onto polyvinylidene fluoride membranes ...
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bioRxiv - Cell Biology 2022Quote: ... cells were collected by centrifugation at 500 × g for 5 minutes and resuspended in 1 ml TNE buffer (150 mM NaCl, 2 mM EDTA, 50 mM Tris-HCl, pH 7.4) containing protease and phosphatase inhibitors (Cell Signaling Technology, Danvers, MA), passed through a 25 gauge needle to shear the cells ...
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bioRxiv - Cell Biology 2019Quote: ... Caco-2 cells were incubated overnight at 4°C with primary antibodies (1:100 dilutions with blocking buffer) for GRP78 (Cell Signaling, MA, USA) or CHOP (Santa Cruz ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 μL of empty Dynabeads® Protein A was washed by Citrate-Phosphate Buffer (pH 5.0) and then incubated with 15ul Rabbit Monoclonal anti-UPF1 antibody (Cell Signaling Technology, Cat# 12040) or 5ug Normal Rabbit IgG Polyclonal Antibody (Merck ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 μL of empty Dynabeads® Protein A were washed by Citrate-Phosphate Buffer (pH 5.0) and then incubated with 15ul Rabbit Monoclonal anti-UPF1 antibody (Cell Signaling TECHNOLOGY, Cat# 12040) or 5ug Rabbit polyclonal anti-phospho-Upf1 (Ser1127 ...
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bioRxiv - Physiology 2020Quote: ... Liver samples (∼100 mg) were homogenized on ice for 60 seconds in 10X (v/w) Cell Signaling Lysis Buffer (Cell Signaling, Danvers, MA) with protease and phosphatase inhibitors (Boston BioProducts ...
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bioRxiv - Physiology 2020Quote: ... Liver samples (∼50 mg) were homogenized and on ice for 60 seconds in 10X (v/w) Cell Signaling Lysis Buffer (Cell Signaling, Danvers, MA) with protease and phosphatase inhibitors (Boston BioProducts ...
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bioRxiv - Molecular Biology 2020Quote: Cell were washed twice with phosphate-buffered saline and lysed with sonication in radioimmunoprecipitation assay (RIPA) buffer (#9806, Cell Signaling, Danvers, MA, USA), before being incubated on ice for 30 min ...
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bioRxiv - Cancer Biology 2019Quote: Western blotting was performed using a routine protocol after cell lysis in RIPA buffer including protease and phosphatase inhibitors (#9806 and #5872 Cell signaling, Frankfurt, Germany). Grayscale intensity values were normalized to internal positive controls and measured using ImageJ software (NIH ...
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bioRxiv - Molecular Biology 2021Quote: Cells were washed twice in phosphate-buffered saline (PBS) (WELGENE, Kyunggi-do, Korea) and lysed in lysis buffer (Cell signaling, Danvers, MA, USA) with protease inhibitor (Cell signaling ...
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bioRxiv - Biochemistry 2021Quote: ... boiled in sample buffer for 5 min and subjected to immunoblot assay with anti-HA antibody (Cell Signaling Technology, Inc., #3724, Danvers, MA).
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked using Odyssey blocking buffer and incubated in primary antibodies overnight (αTubulin, 1:1000, Cell Signaling; SIRT3, 1:1000, Cell Signaling; MCAD 1:1000, Cell Signaling; GCN5L1 1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were probed overnight at 4°C in Odyssey Blocking Buffer with antibodies recognizing PS6 (Ser240/244; rabbit monoclonal; 1:1000; Cell Signaling, Danvers, MA), total S6 ribosomal protein (mouse monoclonal ...
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bioRxiv - Cell Biology 2021Quote: ... Biotinylated internalized proteins in lysates were captured by NeutrAvidin beads (Pierce) and eluted by incubating in Blue Loading Buffer (50 mM DTT, Cell Signaling, Danvers, MA) containing 50 mM DTT at 95°C for 5 min ...
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bioRxiv - Biochemistry 2022Quote: ... Then the cells were washed with cold phosphate-buffered saline (PBS) and lysed using RIPA buffer with protease inhibitor cocktail (#5871S, Cell Signaling, Danvers, MA) and phosphatase inhibitor cocktail (#78420 ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... The processed tissue was rinsed 3 times with PBS (20min each) to wash off the Dent’s Fix and then incubated with primary antibody in blocking buffer (pS6 Cell Signaling 2215, 1:1000) at 4°C with gentle rotation for 5 days ...
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bioRxiv - Immunology 2022Quote: ... Cells were stained for 1hr in permeabilization buffer with Myc antibody (Myc/n-Myc D3N8F Rabbit mAb PE Antibody, #35876, Cell Signaling, 1:50). Myc expression (expressed as geometric MFI ...
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bioRxiv - Immunology 2023Quote: ... At 4 hpi media was removed from the well by pipetting and the neutrophils were lysed in 30uL of 1X RIPA buffer (Cell Signaling Technology, 9806) containing cOmplete EDTA-free protease inhibitor cocktail (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then blocked with immunofluorescence (IF) block buffer (3% BSA in PBS) for 1 hour and incubated with primary antibodies: rabbit anti-γH2A.x (Cell Signaling 9718, 1:2000), rabbit anti-RPA32 (GeneTex GTX113004 1:2000) ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were then suspended in 50 µl Na-Phosphate buffer and incubated at room temperature for 10 minutes with desired antibody [20 µl of CTCF antibody (Cell Signaling Technology #2899) or 11 µg of anti-rabbit IgG (Thermo)] ...
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bioRxiv - Genetics 2023Quote: MEFs were washed twice with cold 1x PBS and lysed using RIPA buffer with a protease/phosphatase inhibitor cocktail (Cell Signaling Technology 5872) to obtain total protein samples ...
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bioRxiv - Cancer Biology 2023Quote: Adherent cells were washed in 1x PBS and lysed on the plate by the addition of complete RIPA buffer with protease (Cell Signaling Technology #5871S) and phosphatase (Cell Signaling Technologies #5870S ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were harvested at indicated time points and lysed in 1x cell lysis buffer (Cell Signaling Technologies, Inc, Danvers, MA, USA; Cat#9803) or 1× radioimmunoprecipitation assay (RIPA ...
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bioRxiv - Molecular Biology 2023Quote: Cells were washed with D-PBS (-) (Wako, 045-29795) and lysed using 140 µL of 10× Cell Lysis buffer (Cell Signaling Technology, 9803S) with PMSF (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then immunoblotted over night at 4°C with the indicated primary antibodies diluted in blocking buffer: Phosphorylated Stat1 (Tyr701) (D4A7) (Cell Signaling, 1:1000), STAT1 (Cell Signaling ...
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bioRxiv - Cell Biology 2023Quote: ... then with Duolink® In Situ Wash Buffer-A and counterstained with Alexa flour 488 conjugated E-cadherin antibody (3199S; Cell Signaling Technology) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... or 5% non-fat dry milk in TBST for 1 h then incubated overnight at 4°C in blocking buffer containing antibodies against TMEM106B (1:500, Cell Signaling Technology 93334), TDP-43 (1:1,500 ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked in 5% non-fat dry milk in TBST for 1 h and then incubated overnight at 4°C in blocking buffer containing the antibody against TMEM106B (1:500, Cell Signaling Technology 93334). The secondary antibody ...
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bioRxiv - Immunology 2024Quote: ... cells were fixed and permeabilized with the eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set (Thermo) for 30 minutes followed by staining with GATA6-PE (Cell Signaling Technologies) at 1:400 for 30 minutes on ice ...