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Citations for Cell Signaling Technology :
851 - 900 of 1318 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The membranes were washed with PBS 3 times for 5 minutes and then incubated with horseradish peroxidase conjugated secondary antibody (Cell Signaling Technology, 7074, 7076) in 5% non-fat milk in PBST at room temperature for 1 hour ...
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bioRxiv - Developmental Biology 2024Quote: ... The membranes were then washed and incubated in secondary antibody (Anti-mouse or Anti-rabbit IgG, HRP-linked Antibody, Cell Signaling 7076S or 7074S) for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was incubated at 4°C overnight with the following primary antibodies: anti-phospho-MEK1/2 (Cell Signaling Technology, Inc., MA, USA, #9122), anti-MEK1/2 (Santa Cruz biotechnology ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was blocked with 2%BSA overnight and was probed with antibodies against goat anti-FLAG (1:1000, Cell Signaling Technologies, D6W5B, #14793), alpha Tubulin (1:10000 ...
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bioRxiv - Physiology 2024Quote: ... membranes were washed 3 times for 5 minutes and incubated for 1 hour in HRP-conjugated anti-rabbit antibody (Cell Signaling Technology, London, UK) at a dilution of 1:20,000 in non-fat dry milk ...
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bioRxiv - Physiology 2024Quote: ... membranes were washed 3x5 min in TBST and incubated for 1 h with HRP-conjugated anti-rabbit IgG (Cell Signaling Technology, Cat No. 7074), HRP-conjugated anti-mouse IgG (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2024Quote: ... the membranes were incubated at 4°C overnight with primary antibodies rabbit anti-RIPK2 (1:1000; Cat. No. 4142; Cell Signaling Technology, Danvers, MA), rabbit anti-iNOS (1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were stained for β-galactosidase using the activity kit (Kit 9860S) from Cell Signaling Technologies according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The pull-down assay of ZFP982-MYC tagged protein was performed through co-Immunoprecipitation (co-IP) using MYC-Tag (9B11) antibody (#2276, Cell Signaling). For MYC-co-IP ...
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bioRxiv - Immunology 2021Quote: ... anti-β-actin (3700) and the secondary antibodies used for western blot and immunofluorescence assay were purchased from Cell Signaling Technology. Anti-SYVN1 (ab170901 ...
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bioRxiv - Neuroscience 2021Quote: Pericytes viability was assessed using the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay according to the manufacturer’s procedure (Cell Signaling Technology). Cells were plated at the appropriate concentration in 96-well plates under different experimental conditions ...
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bioRxiv - Molecular Biology 2022Quote: ... For immunoprecipitation assays, cell lysates were incubated with Protein G Agarose (cat # 20398, Pierce) and anti-Flag antibody (cat # 2368, Cell Signaling) at 4°C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... were used in immunofluorescence staining and Western blot assays: Ki-67 (AB_2797703) (mouse mAb, Cell Signaling Technology, Cat# 9449, 1:800), p-S6S235/S236 (AB_10695457 ...
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bioRxiv - Immunology 2024Quote: ... In vitro ADC cytotoxicity against these cell lines was assessed using a 72-hour XTT viability assay per the manufacturer’s recommendations (Cell Signaling Technology) and as described12.
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bioRxiv - Synthetic Biology 2024Quote: Antibodies used for live cell flow cytometry assays include Alexa Fluor 647-Anti-Myc tag conjugated antibody (Cell Signaling Technologies #2233S) used at 1:100 ...
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bioRxiv - Pathology 2024Quote: ... WB was conducted as described above for translation assay and staining was performed with 1:500 anti-IRE1α (#3294, Cell Signaling), 1:700 anti-pIRE1α (NB100-2323 ...
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bioRxiv - Molecular Biology 2023Quote: ... Then were processed with commercial SA-β-galactosidase staining kits following provider instructions (Kit #9860 from Cell signaling).
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bioRxiv - Biochemistry 2022Quote: ... the membrane was washed four times with PBS-T and incubated with DyLight 800 goat anti-rabbit IgG (Cell Signaling Technology, cat. no. 5151, RRID:AB_10697505) diluted 1:10,000 in PBS-T containing 5% (w/v ...
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bioRxiv - Cancer Biology 2022Quote: ... the membranes were incubated overnight at 4°C with gentle agitation in primary antibody dilution buffer: phosphorylated ALK (Y1604) (Cell Signaling Technologies, #3341, 1:1000), ALK (Cell Signaling Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were transferred to a PVDF membrane and blocked for one hour in 5% dry milk before incubation with KIF3A (D7G3) rabbit monoclonal antibody (Cell Signaling Technology, Danvers, MA, #8507) in TBS containing 0.1% Tween-20 and 5% bovine serum albumin (BSA ...
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bioRxiv - Physiology 2021Quote: ... Membranes were probed for the vascular content of COX-1 (anti-COX-1, cat#: 4841S, source: rabbit, 1:2000; Cell Signaling Technology, Danvers, MA, USA) and COX-2 (anti-COX-2 ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were resolved on an 12% SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membrane for detection with the following antibodies: anti-Glucocorticoid Receptor D6H2L (1:1000 dilution, Cell Signaling, New England BioLabs #12041), anti-PAX7c (1:500 dilution ...
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bioRxiv - Physiology 2023Quote: ... we loaded 25 µg protein of each sample to SDS-PAGE gels and incubated the membranes with 1:1000 eIF2α antibody (Cell Signaling Technology #9722, Danvers, MA) or 1:1000 Phospho-eIF2α antibody (Cell Signaling Technology #9721 ...
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bioRxiv - Genomics 2023Quote: ... Membranes were then washed with PBST and incubated with HRP-conjugated secondary antibodies (Santa Cruz Biotechnology Inc., catalog sc-2004; Cell Signaling Technology, catalog 7076S) at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were dissolved with 3% BSA in tris buffered saline (TBST) and membranes were incubated overnight at 4°C with SQSTM1/p62 (Cell Signaling, cat# 88588, 1:1000). The primary LC3 A/B (Cell Signaling ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were blocked with 1% BSA for 1 h at 37°C and incubated with primary antibodies: anti-Flag (Cell Signaling, D6W5B, 1:1000 dilution), anti-GFP (Abcam ...
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bioRxiv - Genetics 2024Quote: ... Membranes were blocked in 5 % milk for 1 hour followed by overnight incubation with PTEN primary antibody (Cell Signaling Technology, cat# 9188; 1:1000 dilution) or GAPDH primary antibody (Santa Cruiz Biotechnology ...
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bioRxiv - Cancer Biology 2022Quote: ... EMT kit (Cell Signaling Technology, 1:2000), beta-actin (Santa Cruz Biotech,1:4000) ...
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bioRxiv - Immunology 2023Quote: ... with the SimpleChIP Plus kit (Cell Signaling). Sequencing libraries were prepared from isolated plasmids as for MPRA and sequencing on a MiSeq (50bp ...
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bioRxiv - Immunology 2024Quote: ... using an immunofluorescence kit (Cell Signaling Technologies). Samples were stained with DAPI (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... with the SimpleChIP Plus kit (Cell Signaling). The ratio of rs2836882 alleles in the PU.1-bound DNA was quantified in duplicate by TaqMan genotyping (assay C 2601507_20) ...
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bioRxiv - Immunology 2021Quote: ... Western blotting was used as the output assay to determine levels of the ribosomal protein S6 kinase (p-S6K) using an S6K and p-S6K antibodies (Cell Signaling Technology). To assess the impact of mTOR1 inhibition rapamycin (20ng/mL ...
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bioRxiv - Microbiology 2020Quote: ... Transfected cells were selected by culture in the presence of puromycin for 48 hours and either used in HIV-1 replication assays or lysed for immunoblot analysis to measure IRF8 expression using a rabbit monoclonal antibody (Cell Signaling Technology).
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bioRxiv - Microbiology 2020Quote: ... Transfected cells were selected by culture in the presence of neomycin for 48 hours and then used for HIV-1 replication assays or lysed for immunoblot analysis to measure IRF8 expression using a rabbit monoclonal antibody (Cell Signaling Technology).
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bioRxiv - Microbiology 2020Quote: ... and either used in HIV-1 replication assays or lysed for immunoblot analysis to measure MyD88 and TRIF expression using a rabbit monoclonal antibody to MyD88 (Cell Signaling Technology), a rabbit polyclonal antibody to TRIF (Cell Signaling Technology) ...
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bioRxiv - Neuroscience 2021Quote: ... Total protein concentration was determined by a BCA assay (Pierce) 60 μl Protein A beads with 50% slurry (Cell Signaling Technology, #9863S) were then added and incubated for 6 hours to pull down the antibody-protein complex ...
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bioRxiv - Cancer Biology 2022Quote: ... were used in Western blot assays in xenograft pharmacodynamic (PD) studies: SYK (AB_2687924) (rabbit mAb, Cell Signaling Technology, Cat# 13198, 1:1000), EGFR (AB_2230881 ...
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bioRxiv - Bioengineering 2023Quote: ... Radioimmunoprecipitation assay buffer (RIPA) 10x and the ROCK inhibitor Y-27632 (cat# 13624S) were purchased from Cell Signaling Technologies (Whitby, ON, Canada). BrainPhys™ Neuronal Medium was obtained from STEMCELL Technologies (Vancouver ...
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bioRxiv - Immunology 2022Quote: ChIP assays for histone modifications were performed as described previously (Aoyama et al., 2018) using an anti-H2AK119ub1 (#8240S; Cell Signaling Technology) and an anti-H3K27me3 (#07-449 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were harvested at indicated time points and lysed in 1× radioimmunoprecipitation assay (RIPA) buffer (Cell Signaling, Danvers, MA, USA; Cat# 501015489) containing 1× protease inhibitor cocktail (Sigma ...
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bioRxiv - Neuroscience 2020Quote: Membranes were then incubated 24-48 hours at 4°C in primary antibodies – PERK (1:500 anti-Rbt; Cell Signaling Technologies [CST], Danvers, MA; cat # 3192S), IRE-1α (1:500 anti-Rbt ...
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bioRxiv - Bioengineering 2023Quote: ... Proteins were transferred onto nitrocellulose membranes for immunoblotting and then probed with antibodies for phospho-AMPKα (Thr172) and β-Actin (Cell Signaling Technology, Danvers, MA, USA). The membrane was probed with appropriate secondary antibodies and imaged on an Odyssey Imaging System (LICOR ...
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bioRxiv - Molecular Biology 2023Quote: The membranes were blocked with 5% BSA at room temperature for 30 min and incubated with anti-eNOS (clone: D9A5L, Cell Signaling Technology, MA, USA, 1:1,000) and anti-Ser177 p-eNOS (clone ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The membranes were probed overnight at 4 °C with the specified primary antibodies at the dilution of 1:1000 (Cell Signaling Technology: RIPK1 Rabbit mAb#3493)) or 1:4000 (β-actin Rabbit mAb#4970)) ...
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bioRxiv - Cell Biology 2024Quote: ... The immunoblots were conducted using Polyvinylidene Fluoride (PVDF) membranes and were incubated for around 16 h with primary antibodies such as anti-p27KIP1 (Cell Signaling; distributed by EuroClone; cod. #3698), anti cdc2 (Santa Cruz Biotechnologies ...
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bioRxiv - Developmental Biology 2021Quote: ... A SignalStain DAB Substrate Kit (Cell Signaling Technology) was used for chromogenic antibody detection ...
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bioRxiv - Neuroscience 2023Quote: ... and detected using ECL detection kit (Cell Signaling). A summary of antibodies used is presented in Supplementary Information Table S3.
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bioRxiv - Physiology 2023Quote: ... and cAMP ELISA kit (Cell Signaling Technology, #4339) were used to measure these cyclic nucleotide second messengers from adult A ...
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bioRxiv - Cancer Biology 2022Quote: ... Senescence β-Galactosidase Staining Kit (Cell Signaling Technology), according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Senescence β-Galactosidase Staining Kit #9860 (Cell Signaling) was used as per manufactureŕs protocol ...