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Citations for Cell Signaling Technology :
751 - 800 of 1318 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were blocked in skim milk or BSA and probed overnight with the following antibodies: Anti-AML1 (Cell Signaling Technology, Cat# 4336); anti-acetyl-histone H3 (Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... the antibody labeling steps were repeated on the same membrane for the rabbit anti-β-Actin antibody (Cell Signaling Technology Cat# 4967). The β-actin antibody was diluted 1:1000 in blocking buffer ...
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bioRxiv - Cell Biology 2022Quote: ... membranes were incubated with the secondary antibody for 1h at room temperature (rabbit-IgG HRP-linked 1:10000, Cell Signaling Technology (#7074), mouse-IgG HRP-linked 1:10000 ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were saturated and blots were probed with the following primary antibodies: 1:700 rabbit anti-βArr2 (Cell signaling Cat. No. 3857); 1:1000 rabbit anti-GSK3β (Cell signaling Cat ...
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bioRxiv - Cell Biology 2022Quote: ... After blocking the membranes were incubated cold overnight with primary antibodies diluted in Intercept (TBS) Blocking Buffer (E-cadherin mAb #14472 Cell Signaling Technology 1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers ...
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bioRxiv - Immunology 2022Quote: ... Membranes were then washed and incubated in blocking buffer containing HRP-linked horse anti-mouse (1:5000; Cell Signaling Technology Cat#7076) for 45 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers ...
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bioRxiv - Pathology 2021Quote: ... The separated proteins were transferred onto a nitrocellulose membrane for detection with polyclonal rabbit anti-Phospho-Myosin Light Chain 2 (Ser19) antibody (Cell Signaling Technologies) or monoclonal rabbit anti-Myosin Light Chain 2 (D18E2 ...
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bioRxiv - Microbiology 2021Quote: ... membranes were incubated at 4°C overnight with the following primary antibodies: rabbit anti-ubiquitin (1:1000; Cell Signaling Technology no. 3933), rabbit anti-phospho-eIF2α (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... The blocked membrane was then incubated with shaking overnight at 4°C with either CD19 antibody #3574 (Cell Signaling; 1:1000 dilution) or Phospho-CD19 (Tyr531 ...
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bioRxiv - Microbiology 2023Quote: ... The phosphorylated proteins were detected by probing the membranes with an anti-phospho-c-Met antibody (Tyr1234/1235, #3077, Cell Signaling Technology) or an anti-phospho-EGFR antibody (Tyr1068 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated overnight at 4°C with various antibodies: rabbit anti-pan ubiquitin (1:1,000, #3933S; Cell Signaling Technology, Danvers, MA), rabbit anti-K48-linkage specific polyUb (D9D5 ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked using Intercept Blocking Buffer and incubated overnight in the following primary antibodies: rabbit GAPDH (2118S) from Cell Signaling Technology; rabbit BLOC1S1 (19687-1-AP ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were then incubated with the appropriate secondary HRP-linked antibody as follows: anti rabbit-HRP (1:3000, Cell Signaling Technology 7074) for NEMO ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were then washed in TBS-T (3 × 5 min) and probed with secondary antibodies: mouse (Cat #: 7076S; Cell Signaling Danvers, MA) or rabbit (Cat # ...
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bioRxiv - Biochemistry 2022Quote: ... gels were transferred to Nitrocelllose membranes and probed with anti-CFTR (ab596 from Cystic Fibrosis Foundation, 1:1750) or anti-Nav 1.5 (D9J7S, Cell Signaling, 1:5000) overnight in 5% milk in TBST ...
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bioRxiv - Molecular Biology 2024Quote: ... To normalize the phospho (Thr389)-p70 S6 Kinase signals membranes were stripped and reprobed with a phosphorylation-independent anti-p70 S6 Kinase antibody (clone 49D7, Cell Signaling Technology). The expression of the “classical” housekeeping genes β-actin and GAPDH is regulated during myogenesis (Hildyard & Wells ...
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bioRxiv - Immunology 2024Quote: ... The membranes were probed overnight at 4°C with rabbit monoclonal antibodies against inducible nitric oxide synthase (iNOS; Cell Signaling Technology, 2982) or arginase-1 (Arg-1 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... membranes were washed in TBST and incubated for 60 minutes with rabbit horseradish peroxidase (HRP)-conjugated secondary antibody (Cell Signaling Technology 7074) diluted at 1:2000 in 5% milk-TBST ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was further incubated for 1 h at room temperature with secondary goat anti-rabbit horseradish peroxidase antibody (Cell Signaling Technology) at 1:10,000 in 5% BSA-TBST ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were then cut and incubated with different primary antibodies diluted in 5% milk at 4°C overnight: αH3K27me3 (Cell Signaling C36B11) was used at 1:5000 dilution ...
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bioRxiv - Biophysics 2023Quote: ... The membranes were incubated overnight at 4 °C with primary antibodies against VDAC1 and β-Actin (1:5000, #2146; Cell Signaling Technology) after the washing step with secondary antibodies HRP-conjugate Goat anti-Mouse IgG (H+L ...
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bioRxiv - Immunology 2022Quote: ... Membranes were washed 3 times with TBST and then incubated with peroxidase-conjugated secondary antibody (Cell Signaling Technology, Cat.No. 7074S, 1:3000). The antigen-antibody reaction was visualized by enhanced chemiluminescence assay (ECL ...
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bioRxiv - Cell Biology 2022Quote: ... the membranes were incubated with antibodies against Vinculin (E1E9V) XP® Rabbit mAb (1:1000; #13901, Cell signaling technologies, Danvers, MA, USA), Sestrin2 (1:1000 ...
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bioRxiv - Bioengineering 2022Quote: ... the membrane was incubated overnight at 4°C or with primary antibody solution (1:1000 dilution for anti-Alix [E4T7U, Cell Signaling Technology] ...
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bioRxiv - Microbiology 2022Quote: ... membranes were washed with TBST and incubated for 1 hour at room temperature with anti-β-Actin antibody (Cell Signaling Technology #3700) diluted 1:1,000 in PBS antibody buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with primary antibodies diluted in blocking buffer over night at 4°C (SHIP1, 1:500 dilution, Cell Signaling Technologies; IBA1 ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were incubated with appropriate primary antibodies (phospho-ATM, 1:1000, Cell Signaling Technology, #13050; E-Cadherin, 1:1000, Cell Signaling Technology, #3195 ...
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bioRxiv - Biochemistry 2023Quote: ... before being transferred to a nitrocellulose membrane and stained for biotin using HRP-conjugated Anti-biotin (1:1000 dilution, Cell Signaling Technology). WesternBright ECL HRP substrate (Advansta ...
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bioRxiv - Plant Biology 2024Quote: ... The membranes were incubated overnight in agitation at 4°C with Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (#4370; Cell Signaling Technology) in a dilution 1:1000 with PFBS ...
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bioRxiv - Physiology 2024Quote: ... which was blocked using Odyssey blocking buffer (Li-Cor, USA). Membranes were incubated with primary antibodies (GAPDH, Cell Signaling, Cat. 97166 [D4C6R], mouse mAb, 1:1000; (A-Tubulin, Cell Signaling, Cat ...
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bioRxiv - Neuroscience 2024Quote: ... The proteins in the gel were then electrotransferred to polyvinylidene (PVDF) membranes and immunoblotted using an antibody against Psma2 (1:1000 dilution [rabbit]; Cell Signaling #2455) or Rpt6 (1:1000 dilution [mouse] ...
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bioRxiv - Bioengineering 2024Quote: ... membranes were washed three times with TBS-T and incubated with HRP-conjugated goat anti-mouse (1:1,000, Cell Signaling Technology #7056) or goat anti-rabbit (1:1,000 ...
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bioRxiv - Bioengineering 2024Quote: Membranes were subsequently incubated overnight at 4°C with the following primary antibodies: rabbit anti-GAPDH (1:1,000, Cell Signaling Technology #2118) or rabbit anti-HTT ...
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bioRxiv - Synthetic Biology 2024Quote: ... The membrane was blotted with 1:1,000 dilutions of antibodies for phospho-p44/42 MAPK (phospho-Erk1/2; Cell Signaling Technology #9101), p44/42 MAPK (Erk1/2 ...
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bioRxiv - Molecular Biology 2024Quote: ... Subsequently membranes were incubated with primary antibodies (1:500 rabbit anti-LEDGF-PWWP [Abcam; ab177159], 1:1000 rabbit anti-MeCP2 [Cell Signaling Technology; 3456S] ...
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bioRxiv - Neuroscience 2024Quote: ... 10 μg of protein was transferred to 0.45 μm PVDF membrane and probed with rabbit anti-OGT IgG (Cell Signaling, #24083; RRID:AB_2716710) primary antibody at 1:1500 in 5% milk overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was then incubated under soft agitation overnight at 4°C with the primary antibodies for DNMT1 (Cell Signaling Technology, 5032) and β-actin (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2022Quote: Protein samples were collected and lysed using a radioimmunoprecipitation assay buffer containing protease and phosphatase inhibitors (Cell Signaling Technology). Lysates were quantified by Bradford assay (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: ... The primary antibodies used in these assays were: GAPDH (cat. 2118, 1:5000, Cell Signaling Technology, Danvers, MA, USA), YAP/TAZ (cat ...
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bioRxiv - Biochemistry 2024Quote: ... immunoprecipitation assays were performed for 3-h at 4°C using anti-cMyc (Cell Signaling technology, catalog no. 2276), anti-HA (Cell Signaling technology ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The membranes were blocked for 2 hours at room temperature and then incubated with rabbit monoclonal anti-pERK1/2 antibody (Cell Signaling Technology, USA) and anti-rabbit horseradish peroxidase-conjugated secondary antibody (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were further washed with PBS-T buffer and incubated with secondary antibodies (Cell Signaling Technology, #14708 or #14709, 1:10,000 dilution) for 2 hrs at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were blocked and then incubated with primary antibodies (anti-H3K27ac, abcam; anti-H3, Cell Signaling Technology; anti-β Actin, Cell Signaling Technology) at 4°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... Washed membranes were incubated with HRP-conjugated anti-rabbit or anti-mouse secondary antibodies as indicated (1:2000, Cell Signaling Technology, Beverly, MA) in 10 ml of blocking buffer with gentle agitation for 1 h at room temperature ...
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bioRxiv - Synthetic Biology 2021Quote: ... Membranes were then incubated overnight at 4°C with rabbit anti-phosphorylated ERK1/2 antibody (Cell Signaling Technology; 9101S; LOT: 30; 1:1,000) in TBST containing 5% (w/v ...
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bioRxiv - Neuroscience 2020Quote: ... The membranes were probed overnight at 4°C using primary antibodies against p-eIF2α S51 (rabbit, Cell Signaling cat no: 9721, 1:500), anti-t-eIF2α (rabbit ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked followed by overnight incubation at 4°C with primary antibodies: C-terminal Parkin antibody (PRK8; Cell Signaling, 1:40,000 dilution) and N-terminal Parkin antibody (https://mrcppureagents.dundee.ac.uk/reagents-view-antibodies/589302 ...
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bioRxiv - Immunology 2022Quote: ... the membrane was incubated for one hour at RT with peroxidase-conjugated goat anti-rabbit secondary antibody (Cell Signaling Technology, #7074, 1:1000) and washed again ...