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Citations for Cell Signaling Technology :
801 - 850 of 1318 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Luminescence signals on the membranes were detected by an image reader (LAS-4000 system; Fujifilm) with SignalFire ECL Reagent (Cell Signaling Technology; #6883S). The obtained images were processed by Multi Gauge version 3.1 software equipped with the LAS-4000 system.
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bioRxiv - Neuroscience 2020Quote: ... transferred onto nitrocellulose membranes and detected by immunoblot using the following primary antibodies: mGlu5 (rabbit clone D6E7B from Cell Signaling, dilution 1:1000), actin (mouse clone from DSHB ...
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bioRxiv - Cell Biology 2020Quote: ... Blocked membranes were then incubated overnight with a 1:1000 dilution of Phospho-AMPKα (Thr172) (40H9) Rabbit mAb (#2535, Cell Signaling, Massachusetts, USA). Detection was carried out by incubating with a 1:5000 dilution of a goat anti-rabbit antibody conjugated to peroxidase (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... The membranes were blocked with 5% skimmed milk and incubated with primary antibodies (rabbit anti-PPAR-γ antibody, Cell Signaling Technology, 1:1000) and HRP-conjugated goat anti-rabbit IgG ...
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bioRxiv - Molecular Biology 2020Quote: ... The clear supernatant was separated on SDS-PAGE and transferred to PVDF membrane and probed with polyclonal phosphor-Ser/Thr epitope antibodies (Cell Signaling Technology, USA) as described earlier (Rajpurohit et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were separated and on 10% SDS-PAGE gel and blotted onto PVDF membrane and probed with polyclonal phospho-Ser/Thr antibodies (Cell Signaling Technology, USA). Signals were detected using anti-rabbit IgG conjugated with alkaline phosphatase and the color reaction substrates NBT-BCIP (Roche Biochemicals ...
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bioRxiv - Genetics 2021Quote: ... Membranes were rinsed with TBST for 30 min before incubation with horseradish peroxidase-conjugated anti-rabbit secondary antibody (Cell Signaling; 7074S, 1:5000) for 1 h ...
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bioRxiv - Developmental Biology 2021Quote: ... Blocked membranes were incubated overnight at 4°C with primary antibodies diluted in blocking solution: rabbit anti-Ezh2 (Cell Signaling Technologies; 1:1000), rabbit anti-H3K27me3 (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were then washed three times with TBST and incubated in secondary antibodies accordingly: anti-mouse HRP-linked IgG (Cell Signaling Technology, 7076S) or anti-rabbit HRP-linked IgG (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... the membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (anti-mouse-HRP, Cell Signaling #7076; anti-rabbit-HRP, Cell Signaling #7074) in blocking solution (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... were then added to the membrane and incubated overnight at 4°C: anti-Calnexin (1:1,000 dilution, clone C5C9, Cell Signaling Technology, Danvers, MA), anti-Mitofilin (1:500 dilution ...
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bioRxiv - Cell Biology 2024Quote: ... the membranes were incubated overnight at 4°C with the following primary antibodies: anti-AMPKα (Cell Signaling Technology, USA; diluted at 1:1000), anti-p- AMPKα (Thr172 ...
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bioRxiv - Pathology 2024Quote: ... Ten mg of proteins were subjected to SDS-PAGE and transferred onto a nitrocellulose membrane which was probed with antibodies against p-ACC (#3661, Cell Signaling, 1:1000), ACC (#3676 ...
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bioRxiv - Immunology 2023Quote: ... The membranes were then washed three times with TBST and incubated with HRP-linked anti-rabbit IgG antibody (Cell Signaling Technology, catalog # 7074) in TBST containing 5% BSA for 1 h at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... mAb #3288) and Lysosome Associated Membrane Protein 1 (LAMP1) specific rabbit monoclonal antibody (cat. no. mAb #9091) were purchased from Cell Signaling Technology (USA). Anti-caveolin-1 rabbit polyclonal antibody was acquired from BD Biosciences (USA ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were incubated overnight with primary monoclonal anti-Myosin IIa (clone E7Y9O) at the recommended concentration in blocker (Cell Signaling Technology, MA, USA) followed by anti-rabbit IgG HRP linked secondary antibody at 1:10,000 in TBS-T for 1h at RT (antibodies were from Cell Signaling Technology ...
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bioRxiv - Immunology 2023Quote: ... Membranes were incubated for 1 h at room temperature with appropriate secondary antibodies: goat anti-rabbit (1:2000, Cell Signaling Technology, Cat. 7074), goat anti-rat (1:10000 ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was blocked in 5% skimmed milk and incubated overnight at 4°C with primary antibodies for UTX (Cell Signaling Technologies #33510), GFP (JL-8 ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were incubated with the appropriate primary antibody overnight followed by horseradish peroxidase-conjugated anti-rabbit (#7074, Cell Signaling Technology, Danvers, MA) secondary antibody at a dilution of 1:2500 for an hour at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... All membranes were additionally normalized for variation in loading by incubation with vinculin or glyceraldehyde-3-phosphate dehydrogenase (GAPDH, both Cell Signaling, Danvers, MA). After overnight incubation ...
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bioRxiv - Microbiology 2024Quote: ... The membranes were blocked and then incubated overnight at 4°C with the primary antibodies: Tubulin (Sigma, Cat.#T6074; Cell signaling, Cat.#2144), HA-tag (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... An equal amount of protein was boiled in 1x XT sample buffer at 95 °C for 10 min and electrophoresed on 4-12% Bis-Tris gel followed by electrotransfer onto PVDF membrane and was probed using antibody against: histone H3 K27Ac (1:500, Cell signaling technology #8173S), citrullinated histone H3 (1:2000 ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were incubated with primary antibody in TBST overnight at 4°C: Phospho-Akt (Ser473) antibody (Cell Signaling Technology, #9271, 1:1000), Akt (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2024Quote: ... chromatin digestion was performed using a kit (ChIP kit #9005, Cell Signaling Technologies, Danvers, MA, USA), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... phospho-KIT Y719 (both Cell Signaling), Ki-67 (Thermo Scientific Neomarkers) ...
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Salinomycin inhibits epigenetic modulator EZH2 to enhance Death Receptors in Colon Cancer Stem CellsbioRxiv - Cancer Biology 2020Quote: ... Magnetic ChIP kit (Cell Signaling Technology), Verso one step RT-PCR kit ...
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bioRxiv - Cell Biology 2020Quote: ... c-Kit (Cell Signaling Technology, 3074S). F-actin was stained with phalloidin (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... Supershift assays were performed by incubating monoclonal antibody (Ab) to specific AP-1 transcription factors c-Jun (60a8, Cell Signaling), c-Fos (9F6) ...
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bioRxiv - Cell Biology 2022Quote: ... adipocytes were treated with assay medium +/-insulin for 10 minutes and harvested in cell lysis buffer (Cell Signaling Technology #9803) with phosphatase inhibitor (Thermo Fisher Scientific 78442) ...
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bioRxiv - Bioengineering 2020Quote: ... BrdU was added to the cells and proliferation was assessed 12h later using a colorimetric BrdU assay (Cell Signaling, Inc.).
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bioRxiv - Microbiology 2021Quote: Kinase assay was performed using recombinant proteins purified from E.coli and commercially available 10X Kinase buffer (Cell Signaling Technology, #9802). Recombinant proteins (N’-His PINK1 and N’-His Ajuba ...
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bioRxiv - Microbiology 2021Quote: ... The antibodies used in the WB assay included anti-PDGFRβ rabbit mAb (1:1000, Cell Signaling Technology, Danvers, CA, USA), anti-JEV NS3 rabbit antiserum (kindly provided by C.J ...
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bioRxiv - Immunology 2020Quote: Cells were collected and lysed on ice for 30 min in 1X radioimmunoprecipitation assay (RIPA) buffer plus (Cell Signaling Technology) with protease and phosphatase inhibitor cocktail (Thermo Scientific) ...
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Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrowbioRxiv - Molecular Biology 2023Quote: ... These cells were then subjected to ChIP assays with primary antibody against ESRRA (10 μl/IP, Cell Signaling Technology #13826) or normal rabbit IgG ...
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bioRxiv - Microbiology 2023Quote: Cell lysis was performed using Radioimmunoprecipitation (RIP) assay buffer (#9806, Cell Signaling Technology, 3 Trask Lane, Danvers, MA 01923, USA) supplemented with protease and phosphatase inhibitor (#5872) ...
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bioRxiv - Developmental Biology 2024Quote: The following antibodies were used in the immunostaining assays: Alexa Fluor® 488 Phalloidin (Cat# 8878, Cell Signaling, 1:200), anti-Myosin (Cat# A4.1025 ...
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bioRxiv - Physiology 2024Quote: ... The following primary antibodies were used for western blot assays: a phosphorylated Akt antibody (1:1,000, Cell Signaling Technology, 4060) and an Akt antibody (1:1,000 ...
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bioRxiv - Immunology 2024Quote: ... HDAC1 activity was measured by ELISA assay detecting the level acetylated Histone 3 (anti-acetil-H3; K27; 8173S; Cell Signaling).
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bioRxiv - Molecular Biology 2020Quote: ... the membranes were washed and incubated with HRP-conjugated anti-mouse or anti-rabbit secondary antibodies (1:3000, Cell Signaling Technology, #7076 or #7074) and followed by detection with ECL Western blot substrate (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... and membranes were blotted using antibodies raised against murine haem oxygenase-1 (HO-1; rabbit polyclonal, 1:1000, Cell Signaling Technology, Leiden, The Netherlands) or superoxide dismutase 2 (SOD2 ...
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bioRxiv - Microbiology 2020Quote: ... blocked with 5% skimmed milk powder at room temperature for 1 h, the membrane was incubated with the target primary antibody with desired dilution (anti-ACE-2, 1:1000 (Cell signaling technology, MA, USA); TMPRSS2 ...
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bioRxiv - Neuroscience 2020Quote: ... the membrane was incubated with the rabbit monoclonal anti-FosB primary antibody (dilution: 1:1000, product number: 2251S, Cell Signaling Technology, Danver, MA, USA) at 4 °C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were blocked in 5% BSA in TBS-T before incubation with primary antibodies: phospho-AMPKα Thr172 (1:1000, Cell signaling, MA, USA #2535), β-actin (1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... After saturation with TBS Tween 5% BSA for 1h and hybridization of the membranes with primary overnight (BCR-ABL Ab3 Calbiochem, pCrkL Tyr207 Cell Signaling, pTYR 4G10 Millipore) and secondary 1h antibodies coupled to HRP ...
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bioRxiv - Cancer Biology 2022Quote: ... The membrane was incubated overnight with primary antibodies and then with secondary antibodies conjugated with horseradish peroxidase (#7074S and #7076S, Cell Signaling, Danvers, MA, USA). Antibodies against p-Src (#2105S) ...
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bioRxiv - Cell Biology 2021Quote: ... the membrane was incubated with HRP-conjugated anti-rabbit IgG or HRP-conjugated anti-rat IgG secondary antibody (Cell Signaling Technology, Danvers, MA, USA) for 2 h ...
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bioRxiv - Immunology 2023Quote: ... Mitochondrial activity was measured after 24 hours by adding 100 nM of a mitochondrial membrane potential-sensitive dye (stock conc.: 1 mM in DMSO, MitoTracker® Deep Red FM, Cell Signaling Technology, USA) to the cells for 30 min at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... The blocked membrane was cut and then incubated with shaking overnight at 4°C with either CD19 antibody #3574 (Cell Signaling; 1:1000 dilution) or GAPDH-HRP Conjugate (Cell Signaling ...
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bioRxiv - Cancer Biology 2023Quote: ... The FGFR1-probed membranes were washed 5x for 5min in TBST and then probed with anti-rabbit HRP-conjugated antibody (7074S, Cell Signaling, Danvers, MA, USA) for 1h at RT ...
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bioRxiv - Immunology 2023Quote: ... The blocked membrane was cut and then incubated with shaking overnight at 4°C with either CD19 antibody #3574 (Cell Signaling; 1:1000 dilution), EAAT3 antibody (Cell Signaling ...