Labshake search
Citations for Cell Signaling Technology :
701 - 750 of 1318 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... transferred to nitrocellulose or PVDF membranes and western blotting was conducted using the following primary antibodies: eIF2α (Cell Signaling Technology #9722), p-eIF2α (Cell Signaling Technology #9721) ...
-
bioRxiv - Physiology 2023Quote: ... Secondary antibodies were diluted in 5% blocking solution and membranes were incubated for 1 h at room temperature (1:1000 donkey anti-rabbit IgG, HRP-linked Antibody, #7074S, Cell Signaling). Proteins were detected based on HRP substrate-based enhanced chemiluminescence (#WBKLS0500 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Membranes were incubated overnight at 4°C with rabbit-anti-human ABCB1 (E1Y7B; mAb #13342; Cell signaling Technology; dilution 1:1000) and mouse-anti-human β-actin (sc-47778 ...
-
bioRxiv - Molecular Biology 2023Quote: ... blotted onto nitrocellulose membranes, and probed with anti-ZSWIM8 (1:400; Cat #PA5-59492, Thermo) or anti-GAPDH (1:1000; Cat #2118, Cell Signaling) followed by HRP-conjugated anti-rabbit secondary (1:10,000 ...
-
bioRxiv - Physiology 2023Quote: ... non-fat milk in tris-buffered saline (TBS)-0.05% Tween (TBS-T), membranes were incubated overnight with primary antibodies against Drosophila pAkt-Ser505 (1:500, #4054) from Cell Signaling, or Actin5c (1:200 ...
-
bioRxiv - Cell Biology 2023Quote: ... proteins were transferred to nitrocellulose membrane (Cytiva) and revealed using following antibodies: rabbit AGR2 (Cell Signaling Technologies, Clone D9V2F, 1/1000), mouse FLAG-M2-HRP (Sigma ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... The membranes were then incubated in the same buffer with anti-TAZ and anti-β -actin antibody (#8418, #5125, Cell Signaling) or anti-IHH antibody (#13388-1-AP ...
-
bioRxiv - Microbiology 2022Quote: ... The membrane was immunoblotted with primary antibody followed by either Anti-mouse IgG HRP-linked Antibody (Cat # 7076S, Cell Signaling Tech) or Anti-rabbit IgG HRP-linked antibody (Cat #7074S ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... membranes were incubated with the following primary antibodies in the blocking buffer: rabbit anti-GAPDH (1:2,500; Cell Signaling Technology #2118) and mouse anti-HiBiT (1.0 µg/ml ...
-
bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to nitrocellulose membranes at 100 volts for 90 minutes in cold 1X transfer buffer (Cell Signaling Technology, 12539S). Membranes were blocked with 5% (w/v ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The membrane was incubated with primary antibodies (rabbit anti-EGFR D38B1 Cell Signaling Technologies, rabbit anti-HER2 2242 Cell Signaling Technologies, rabbit anti-PD-L1 E1L3N Cell Signaling Technologies ...
-
bioRxiv - Molecular Biology 2023Quote: ... membranes were incubated with primary antibodies at 4°C overnight: anti-HIF-1α (1:1000, Cat #36169, Cell Signaling Technology, USA) or anti-β-Actin (1:5000 ...
-
bioRxiv - Microbiology 2022Quote: ... the membrane was washed 3 times with TBS-T and incubated with horseradish peroxidase-conjugated secondary antibody (Cell Signaling Technology #7074) for 1 hour ...
-
bioRxiv - Cancer Biology 2022Quote: ... The separated proteins on the gel were transferred onto PVDF membrane and were probed for specific antibodies against Rb (cat# 9309; Cell signaling) phospho-Rb (cat# 8516 ...
-
bioRxiv - Biochemistry 2022Quote: ... The membranes were then probed with goat anti-rabbit and goat anti-mouse secondary antibodies (#7074, #7076, Cell Signaling, Danvers, MA), and blots were incubated with Clarity Western ECL Substrate (Cat ...
-
bioRxiv - Molecular Biology 2022Quote: ... The membrane was then transferred to a secondary antibody solution containing a 1:5000 dilution of α-mouse HRP (Cell Signaling) for one hour of shaking at room temperature ...
-
bioRxiv - Immunology 2022Quote: ... The membranes were blocked with 5% skim milk and then treated with Trim28 primary and anti–rabbit IgG (Cell Signaling Technology) secondary antibodies ...
-
bioRxiv - Genetics 2022Quote: ... Membranes were blocked in 4% BSA in 1X TBS and probed with S6 ribosomal protein 1:1000 (2217, Cell Signaling Technologies), phosphor-S6 ribosomal protein 1:1000 (2215 ...
-
bioRxiv - Plant Biology 2022Quote: ... the membrane was incubated overnight at 4°C with rabbit anti-phospho-acetyl CoA carboxylase (Ser79) antibody (#3661; Cell Signaling Technology Europe BV ...
-
bioRxiv - Physiology 2023Quote: Expression of specific proteins in polysomal profiles (Fig. 6) was determined by probing the membrane with antibodies against RsL7a (dilution 1:4000) from Cell Signaling; 4EBP (dilution 1:4000) ...
-
bioRxiv - Microbiology 2022Quote: ... Membranes were reblocked with PBS Blocking Buffer and reprobed overnight at 4°C with anti-Akt antibody (Cell Signaling Technology #9272) diluted 1:1,000 in PBS antibody buffer ...
-
bioRxiv - Neuroscience 2023Quote: ... and corresponding polyvinylidene difluoride (PVDF) membranes were incubated overnight at 4°C with various antibodies: rabbit anti-Bcl-2 (1:500; #3498, Cell Signaling), rabbit anti-Bax (1:500 ...
-
bioRxiv - Cell Biology 2023Quote: ... Membranes were washed in PBS and incubated with fluorescent secondary antibody Goat anti-mouse Alexafluor 594 (8890, Cell signaling technology, USA). Membranes were mounted on slides with Fluoromount-G containing DAPI (00-4959-52 ...
-
bioRxiv - Cell Biology 2023Quote: ... Membranes were then incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (1:5000, CS7076, Cell Signaling, Danvers, MA, USA) and goat anti-rabbit IgG (1:5000 ...
-
bioRxiv - Genetics 2023Quote: ... The presence of mitochondrial and cytoplasmic ribosomes in gradient fractions was estimated by incubating resulting membranes with anti-MRPL11 monoclonal antibody (D68F2, Cell Signaling) and anti-RPS6 rabbit monoclonal antibody (Cell Signaling).
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... the membranes were incubated overnight in a cold room with the following primary antibodies: anti-AKT (1:1000, Cell Signaling #9272S), anti-pAKT (1:1000 ...
-
Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... Membranes were then incubated with rabbit monoclonal anti-CtIP antibodies (1:3000 dilution in TBS-T; #9201, Cell Signaling Technology, USA), rabbit monoclonal anti-cleaved caspase-3 (1:3000 dilution in TBS-T ...
-
bioRxiv - Cancer Biology 2024Quote: ... were separated on gradient polyacrylamide gels and transferred to nitrocellulose blotting membrane (0.45uM; GE healthcare Primary antibodies against phospho mTOR (Cell signaling), total mTOR (Cell signaling ...
-
bioRxiv - Molecular Biology 2024Quote: ... membranes were washed for 15 minutes in TBST and incubated with horseradish peroxidase-conjugated anti-mouse IgG (Cell Signaling; cat#: 7076) or IgM (ThermoFisher Scientific ...
-
bioRxiv - Biochemistry 2024Quote: ... Membranes were washed three times and incubated with anti-mouse IgG or anti-rabbit IgG HRP-linked antibodies (Cell Signaling Technology) both diluted in PBST (1:2,000) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Membranes were washed for 10 minutes with PBS-T 3 times and then incubated in DyLight secondary antibodies (Cell Signaling Technologies) for one hour at room temperature ...
-
bioRxiv - Cell Biology 2024Quote: ... The membrane was then washed 3 times in 1x TBST and 1:3000 anti-rabbit secondary antibody (Cell Signaling Technology #7074) in 5% milk TBST was added ...
-
bioRxiv - Cancer Biology 2024Quote: ... the membrane was washed in TBS-T and incubated for 1h with goat anti-rabbit (cat. no. 7074S, Cell Signaling Technology) or goat anti-mouse (cat ...
-
bioRxiv - Cancer Biology 2024Quote: ... Membranes were blocked in 10% milk/TBST and immunoblotted with the following antibodies: rabbit monoclonal c-MYC (Cell Signaling, 1:1000) and Mouse monoclonal anti-b-Actin (Sigma-Aldrich ...
-
Convergent evolution of distinct immune sensor systems for fungal polygalacturonases in BrassicaceaebioRxiv - Plant Biology 2020Quote: ... MAPK activity assay was performed by immunoblotting with anti-phospho p44/42 MAP kinase antibody (Cell Signaling Technology). For gene expression assays ...
-
bioRxiv - Cell Biology 2022Quote: Exosomes isolated by ultracentrifugation were lysed in equal volume of radio-immunoprecipitation assay (RIPA) buffer (Cell Signaling Technology) containing protease inhibitor cocktail (Cell Signaling Technology ...
-
bioRxiv - Developmental Biology 2020Quote: ... Immunofluorescence assays were performed with the following primary antibodies: anti-Trx-1 (1:50, Cat# 24295, Cell Signaling) and anti-eGFP (1:400 ...
-
bioRxiv - Cell Biology 2023Quote: ... Antibodies for western blots and co-immunoprecipitation assays were anti-phospho-p44/42 MAPK (Cell Signaling, Technology, 9101), anti-p44/42 MAPK (Cell Signaling Technology ...
-
bioRxiv - Cancer Biology 2023Quote: ... and Rabbit (DA1E) mAb IgG XP® Isotype Control (15 μL/assay, #66362; Cell Signaling Technology, Danvers, MA). DNA obtained from the CUT&RUN assay was amplified using SYBR Green qRT-PCR with the StepOnePlus™ Real-Time PCR System (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2024Quote: Senescence associated β-galactosidase was measured in cell cultures using a plate-based fluorescent assay (Cell Signaling, 23833). In tissues β-galactosidase activity at pH 6 was determined using either Senescence β-Galactosidase Staining Kit (cell signaling ...
-
bioRxiv - Cancer Biology 2024Quote: ... Total p21Waf1/Cip1 protein concentrations were assayed using a commercially available colorimetric sandwich ELISA assay (Cell Signaling Technology). Data were compared to a standard curve supplied with the ELISA kit.
-
bioRxiv - Cancer Biology 2022Quote: ... Kit (Cell Signaling Technology, 3074), Ido1 (Santa Cruz Biotechnology ...
-
bioRxiv - Plant Biology 2020Quote: ... membranes were incubated with the antibody against Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® (Cell Signaling Technology, USA). As a loading control membranes were incubated with anti-tubulin antibody (Abiocode ...
-
bioRxiv - Cancer Biology 2021Quote: ... The membranes were blocked and incubated overnight at 4 °C with a mouse monoclonal anti-FGFR1 antibody (1:100; Cell Signaling Technology), a rabbit polyclonal anti-p-AKT antibody (1:500 ...
-
bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked in 5% milk and incubated in 2% BSA with primary antibodies to GAPDH (Cell Signaling Technology Cat# 13084, RRID:AB_2713924), Eg5 (Sigma Aldrich Cat# SAB4501650 ...
-
bioRxiv - Pathology 2022Quote: ... The blotted membranes were incubated with the following primary antibodies: rabbit monoclonal anti-phosphorylated Smad2 (Ser465/467) antibody (Cell Signaling Technology, #3108), rabbit monoclonal anti-Smad2 antibody (Cell Signaling Technology ...
-
bioRxiv - Genomics 2020Quote: ... the membrane was incubated with the anti-Phospho-Smad1 (Ser463/465)/Smad5 (Ser463/465)/Smad9 (Ser465/467) (1:100, 13820S, Cell Signaling Technology), anti-HA (1:10000 ...
-
bioRxiv - Molecular Biology 2020Quote: ... After 3 washing steps (5 min each) with PBS-T membranes were incubated with horseradish-linked goat anti-rat (1:2000 dilution; 7077S; Cell Signaling Technology) or goat anti-rabbit (1:2000 dilution ...
-
bioRxiv - Immunology 2020Quote: ... Membranes were then washed 3x with TBST at room temperature then incubated with secondary anti-rabbit HRP-conjugated antibody (Cell Signaling Technology) for 1 hour ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Equal protein loading was verified by stripping off the original antibodies and re-probing the membranes with a different primary antibody such as anti-GAPDH (Cell Signaling Technology). Pertussis toxin (PTX ...