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Citations for Cell Signaling Technology :
8351 - 8400 of 9030 citations for GAPDH Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... the membranes were incubated overnight at 4°C with the following primary antibody: rabbit anti-IKKα (Cell Signaling 2682; 1:1,000), rabbit anti-phospho-IKKα/β (Ser180/Ser181 ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were washed with TBST prior to incubation for 2 hrs at room temperature in the appropriate secondary antibody (anti-rabbit or anti-mouse; Cell Signaling) at 1:2000 dilutions ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were washed three times with TBS containing 0.1% Tween 20 then incubated with anti-Rabbit IgG-HRP conjugated (Cell Signaling: 7074). Images were acquired by chemiluminescence using Clarity ECL (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... goat anti-chicken 488 (Life Technology A11039 at 1/200) and goat anti-rabbit 488 (Cell Signaling Technology #4412 1/200).
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-HA tag (C29F4) monoclonal antibody (1:1000 for NaV1.6 and 1:2000 for NF186; Cell Signaling, cat. no. 3724); mouse anti-ankyrin G antibody (1:50 ...
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bioRxiv - Pathology 2022Quote: ... Then membranes were incubated with peroxidase-conjugated secondary antibodies for 1 h at room temperature: anti-rabbit immunoglobulin G (IgG) (AB_2099233; Cat.#7074, 1:2000, Cell Signaling Technology), anti-mouse IgG (AB_330924 ...
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bioRxiv - Immunology 2022Quote: ... Immunohistochemistry staining using the Monoclonal Rabbit Anti-Mouse CD45 (AB_2799780; clone D3F8Q, cat. no. 70257S, Cell Signaling Technology, Danvers, MA, USA) was performed using the Leica Bond Max Automatic Staining System in a peroxidase-mediated oxidation of 3,30-diaminobenzidine (DAB ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were washed twice with PBST and incubated with HRP conjugated anti-rabbit IgG (1:500, catalog 7074S, Cell Signaling Technology) for 1 h ...
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bioRxiv - Developmental Biology 2022Quote: Larval wing imaginal discs were stained using the following antibodies: Rabbit anti-cleaved Drosophila Dcp-1 (1:300, Cell Signaling Technology), Rabbit-anti-p-eIF2α (1:300 ...
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bioRxiv - Molecular Biology 2022Quote: ... anti-rabbit immunoglobulin G (IgG) (H + L) (Dylight 800 conjugates) and anti-mouse IgG (H + L) (Dylight 680 conjugates) (Cell Signaling) were used as secondary antibodies.
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bioRxiv - Microbiology 2022Quote: ... The secondary antibodies used for immunoblotting were anti-rabbit IgG (H+L) (DyLight™ 800 4X PEG Conjugate) (Cell Signaling, 5151S) and anti-mouse IgG (H+L ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were fixed in ice-cold 70% ethanol and stained with propidium iodide (50 μg/mL)/RNase (100 μg/mL) and rabbit-anti-phospho-HistoneH3-antibody (#9701, Cell Signaling) and goat-anti-rabbit-Alexa-488-conjugated secondary antibody (#R37116 ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were incubated overnight at room temperature (1/100 rat anti-BrdU OBT0030 AbD Serotec; 1/400 rabbit anti-Caspase #9661 Cell Signaling; 1/250 rabbit anti-OTX2 ab92326 Abcam ...
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bioRxiv - Cancer Biology 2019Quote: ... Primary antibodies were the monoclonal anti-PD-L1 antibody raised in rabbit (1:100; E1L3N, Cell Signaling Technology, Danvers, MA, USA), monoclonal mouse anti-PD-1 antibody (1:80 ...
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bioRxiv - Developmental Biology 2019Quote: ... mouse anti-Isl1/2 (Developmental Studies Hybridoma Bank, 1:100) and rabbit anti-β3-tubulin (Cell Signaling Technology, 5568S 1:200).
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bioRxiv - Genetics 2020Quote: ... and bound Myc bead (1/2 total amount) samples were analyzed by SDS-PAGE and immunoblotting with rabbit anti-Myc monoclonal antibody (1:2000; Cell Signaling) to detect Myc-tagged EXOSC5 and EXOSC2 ...
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bioRxiv - Cancer Biology 2019Quote: ... Slides were incubated with the polyclonal cleaved caspase 3 primary antibody (rabbit, 1:100; 9661, Cell Signaling, Frankfurt am Main, Germany) for 60 min at RT followed by ImmPRESS Reagent Kit ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the membranes were blocked with 5% bovine serum albumin in phosphate-buffered saline-0.1% Tween 20 and probed with rabbit anti-PPARγ antibody (#2443S, Cell Signaling Technology) and phospho-ser273 PPARγ (bs-4888R ...
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bioRxiv - Molecular Biology 2020Quote: ... Western blotting was performed according to standard procedures using the following antibodies and dilutions: H3K27me3 (1:1000 Cell Signaling C36B11, rabbit), H3K9me2 (1:1000 Upstate 07-441 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were probed overnight at 4°C using primary antibodies (rabbit anti-p S6 (S235/236) 1:1000 (Cell Signaling #4858), rabbit anti-p-S6 (S240/244 ...
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bioRxiv - Neuroscience 2020Quote: ... including the primary antibodies (mouse anti-Iba1 antibody, Abcam, 1:400; rabbit anti-iNOS antibody, Abcam, 1:50; mouse anti-GFAP antibody, Cell signaling, 1:400 ...
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bioRxiv - Biochemistry 2020Quote: ... anti-rabbit antibody was purchased form Cell Signaling Technology (7074S) and anti-mouse antibody was purchased from Cell Signaling Technology (7076S).
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bioRxiv - Pathology 2020Quote: ... followed by incubation with anti-mouse or anti-rabbit HRP-conjugated secondary antibodies (1:5000, Cell Signaling Technology, Danvers, MA, USA). The samples were normalized with GAPDH (1:5000 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Anti-rabbit IgG (H+L)-F(ab’)2 Fragment-Alexa Fluor 555 Conjugate (cat. No. 4413, Cell Signaling Technology, Beverly, USA), CRLS1 pAb (cat ...
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bioRxiv - Pathology 2020Quote: ... for one hour at 25°C and incubated overnight with the rabbit anti-phospho-MEK1/2 (Ser217/221) antibody (Catalog #9154; Cell Signaling), the mouse anti-MEK1/2 antibody (Catalog #4694 ...
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bioRxiv - Immunology 2020Quote: ... The membranes were then washed and incubated with anti-mouse or anti-rabbit HRP-conjugated secondary antibodies from Cell Signaling Technology and GE Healthcare ...
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bioRxiv - Microbiology 2019Quote: ... and then incubated for 1 h with 1:1,000 dilution of secondary HRP-conjugated goat anti-rabbit IgG antibody (Cell Signaling Technology). Images of the immunoblots were developed with ECLTM Western Blotting Detection Reagent (GE Healthcare).
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bioRxiv - Developmental Biology 2021Quote: ... and 0.05% Tween 20] plus 5% BSA before incubation with the primary antibody (1:1000 for mouse monoclonal 12C11 anti-REST and 1:2000 for anti-LaminB1 rabbit polyclonal, Cell Signaling). Anti-rabbit IgG or anti-mouse IgG conjugated to horseradish peroxidase (1:2000 ...
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bioRxiv - Cell Biology 2019Quote: ... The membranes were then washed and incubated with either mouse or rabbit secondary antibody linked with horseradish peroxidase (Cell Signaling Technologies) and washed ...
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bioRxiv - Bioengineering 2020Quote: ... HRP conjugated goat anti-mouse (1:4000) and goat anti-rabbit (1:4000) secondary antibodies were obtained from Cell Signaling Technology, Danvers ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit polyclonal anti-phospho-myosin light chain 2 (Thr18/Ser19) (for immunostainings on elastomers; 1:200, Cell Signaling; cat. no. #3674). An anti-Plexin-B1 antibody was raised against the extracellular domain of Plexin-B1 ...
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bioRxiv - Cell Biology 2021Quote: ... Phospho-Akt and histone H3 were detected by incubation with rabbit anti-phospho-Akt (Cell Signaling Technologies, #4054, diluted 1:1000) and rabbit anti-histone-H3 (Abcam #1791 ...
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bioRxiv - Cancer Biology 2021Quote: ... and then probed for one hour at room temperature with a 1:3000 dilution of anti-rabbit (Cell Signaling Technology, #7074) IgG HRP-linked antibody and developed using Pierce ECL Western Blotting Substrate (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... The membranes were blocked with 5% non-fat dry milk for 2 h at RT and incubated overnight at 4°C with rabbit anti-phospho-ERK1/2 (1:2000, Cell Signaling), anti-phospho-Ser473-Akt (1:2000 ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were washed with PBS-T and incubated with HRP-coupled goat anti-mouse or anti-rabbit IgG antibodies (Cell Signaling). Detection was performed with Clarity Lightning ECL (Bio Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... Thermo Fischer Scientific MA5-11690) overnight at 4°C and then incubated with goat anti-rabbit HRP linked IgG (1:10000, Cell signaling). Membranes were visualized using a ChemiDoc MP (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... MCF7 cell lysate as prepared above was diluted to 0.8 mg/mL total protein in 1:3 lysis buffer:dilution buffer with 0.5 nM rabbit anti-BRD4 antibody (Cell Signaling Technology; E2A7X) and 1 nM CoraFluor-1-labeled anti-rabbit nano secondary ...
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bioRxiv - Cancer Biology 2020Quote: ... Human-specific EpCam-647 (clone VU1D9, #5447) and rabbit anti-human γH2AX antibodies (Phospho-Histone H2A.X Ser139; clone 20E3, #9718) from Cell Signaling Technologies, and EpCam-APC antibody (clone EBA-1 ...
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bioRxiv - Immunology 2021Quote: ... overnight at 4°C and secondary HRP-conjugated anti-rabbit IgG (1:2,000 in 5% nonfat dry milk; Cell Signaling Technology) for 1 h at RT ...
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bioRxiv - Immunology 2021Quote: ... the membranes were washed with PBST for 30 min before incubating with either the fluorescently conjugated secondary antibodies (1:20000) (Li-COR) or horseradish-peroxidase conjugated goat anti-rabbit secondary antibody for the Nrf2 blot (1:5000, #7074) (Cell Signaling) for 1 h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated overnight in rabbit anti-c-Fos primary antibody (1:200 in blocking solution, Cell Signaling Technologies, Ab #2250). Following overnight incubation in primary antibody ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated with the primary antibodies rabbit anti-phospho-β catenin (Ser675, 1:100, D2F1, Cell Signaling Technology, MA, USA) and mouse anti-β-catenin (1:100 ...
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bioRxiv - Biochemistry 2022Quote: ... The membranes were then probed with goat anti-rabbit and goat anti-mouse secondary antibodies (#7074, #7076, Cell Signaling, Danvers, MA), and blots were incubated with Clarity Western ECL Substrate (Cat ...
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bioRxiv - Neuroscience 2022Quote: ... 10% normal donkey serum in 1xPBS) and then incubated overnight at 4°C with a rabbit polyclonal antiserum against cFos (1:1000, Millipore and Cell Signaling) followed by a Cy3-conjugated donkey-anti-rabbit secondary antibody (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Cancer Biology 2022Quote: ... Rabbit anti-Integrin β1 was a gift from the Albiges-Rizo’s lab. Rabbit monoclonal anti-Integrin β5 (ref. 3629) was purchased from Cell Signaling Technology. Mouse monoclonal anti-integrin αvβ5 (ref ...
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bioRxiv - Cell Biology 2022Quote: ... We also used the following commercial antibodies for western blotting and/or immunofluorescence staining: rabbit anti-WASF3 (#2806; Cell Signaling Technology), mouse anti-ARP3 (ab49671 ...
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bioRxiv - Developmental Biology 2022Quote: ... The antibodies for western blotting were rabbit anti-COFILIN monoclonal antibody (1:1,000 dilution, 5175, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-phospho-COFILIN monoclonal antibody (1:1,000 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... and 1:5000 dilution of HRP-conjugated rabbit anti-mouse secondary antibody (Cat. No. 7076, Lot No. 36, Cell Signaling Technology), and the blot was developed similarly.
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bioRxiv - Cancer Biology 2022Quote: ... 2021) using 25μg of chromatin and 10ug of either anti-HIF1α ChIP antibody (Novus) or anti-rabbit IgG (Cell Signaling Technologies).Chromatin was immunoprecipitated overnight at 4C and HIF1α binding sites assayed via RT-qPCR using 2μl of either input ...
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bioRxiv - Developmental Biology 2022Quote: ... Primary antibodies used in this study were as follows: rabbit monoclonal active β-catenin (Cell Signaling, 19807S, 1:100 with TSA), mouse monoclonal ...