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Citations for Jackson ImmunoResearch :
401 - 450 of 668 citations for 3 hydroxy 20 oxopregn 5 en 21 yl acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and then incubated for 3 hours at room temperature with Cy5-conjugated anti-sheep secondary antibody (Jackson ImmunoResearch, 713-175-147) diluted 1:500 in a solution of 1.5% BSA in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3 times in PBS and immediately transferred to the secondary antibody solution: Cy3 goat anti-mouse (1:500, Jackson ImmunoResearch) for 2 hours at home temperature ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed 3 times (15 minutes) in TBS and incubated with a donkey antirabbit biotinylated secondary antibody (Jackson ImmunoResearch Laboratories) at 1:400 in blocking buffer for 2 hours at room temperature ...
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bioRxiv - Immunology 2024Quote: ... IgG isotype antibodies binding to the diverse antigens were detected with Cy-3 conjugated anti-human IgG (1:1000) from Jackson ImmunoResearch Laboratory ...
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bioRxiv - Physiology 2024Quote: ... blocked with 3 % BSA for 1 hour and incubated with AffiniPure Fab Fragment Goat Anti-Mouse IgG (H + L) (Jackson ImmunoResearch) in PBS (20 µg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... free-floating brain sections were blocked in 3% normal donkey serum for 2 h (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) and then incubated with rabbit anti-DsRed primary antibody (1:1,000 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were permeabilized with 0.3% Triton X-100 in PBS (PBS-T) and blocked with 3% normal donkey serum (Jackson ImmunoResearch, RRID:AB_2337258) in PBS-T (PBS-T DS ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were washed once with FACS buffer and incubated for an additional 20 minutes at room temperature with a donkey anti-mouse Alexa-647-conjugated secondary antibody (1/400, Jackson ImmunoResearch Laboratories). After washing with perm/wash ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated overnight with equal molar amounts of F(ab’)2 goat-anti-mouse IgM+G (αM, 20 μg/ml, Jackson ImmunoResearch Laboratories), hen egg lysozyme (HEL ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The membranes were then washed with phosphate buffered saline containing 0.1% Tween-20 and probed with horseradish peroxidase secondary antibody (1:5,000; goat polyclonal IgG; Jackson ImmunoResearch, West Grove, USA) in blocking buffer containing 0.1% Tween-20 for 1□h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were permeabilized and blocked with 3% BSA containing 0.1% Triton X100 in PBS for 20 mins at RT and labelled with anti-mouse Cy3 antibody (Jackson ImmunoResearch, 1:400) for 1 hr at RT ...
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bioRxiv - Immunology 2020Quote: ... Membranes were incubated in primary antibodies for 1-3 hours at room temperature or overnight at 4°C followed by incubation in 20 ng/ml secondary horseradish-peroxidase (HRP)-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories) for 45 min at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.01% SDS with 1× protease inhibitor cocktail) and then blocked with 200 µl 20 mg/ml BSA (Jackson ImmunoResearch, 001-000-161), 4 µl 10 mg/ml salmon sperm DNA (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then treated with blocking buffer containing 5% normal goat serum (Jackson Immunoresearch, 005-000-121), 0.1% Tween-20 (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... the tissue sections were washed with PBS and blocked with 5% donkey or goat serum (Jackson ImmunoResearch), 2% BSA (Gemini Bio-Products ...
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bioRxiv - Neuroscience 2020Quote: ... CY™5-conjugated donkey anti-rabbit (1/500, v/v; CAT#711-175-152, Jackson ImmunoResearch), and Brilliant Violet™421-conjugated donkey anti-goat (1/500 ...
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bioRxiv - Neuroscience 2022Quote: ... incubated for 90 min in HRP-linked secondary antibody (Jackson ImmunoResearch; 1:10,000 in TBST + 5% BSA), then washed three times with TBST and twice with TBS for 10 min each ...
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bioRxiv - Developmental Biology 2020Quote: Samples were blocked in dPBS with 5% BSA and 1% donkey serum (Jackson Immunoresearch, 017-000-001). Concentration of primary antibodies and % Triton X-100 are listed in the Supplementary table 2 ...
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bioRxiv - Physiology 2022Quote: ... Tissue sections of 10 μM were blocked in 5% Normal Goat Serum (Jackson ImmunoResearch, West Grove, PA) in PBS + 1%BSA + 0.3%Triton X-100 for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Primary antibodies (diluted 1:300) were diluted into 5% normal donkey serum (Jackson ImmunoResearch, 017-000-121) and incubated overnight at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... and blocked with PBS+5% BSA (Fisher) or serum of the host of secondary antibodies (Jackson ImmunoResearch). Coverslips were incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... spinal cord sections were blocked in 5% normal goat serum (NGS; Cat# 005-000-121, Jackson ImmunoResearch) and 0.1% Tween-20 for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were blocked with 5% of the respective serums NGS normal goat serum (AB_2336990, Jackson Immunoresearch) or NDS normal donkey serum (AB_2337258 ...
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bioRxiv - Developmental Biology 2024Quote: ... Fc receptors were blocked in PBS/5% FCS with 250 µg/ml purified IgG (Jackson ImmunoResearch Laboratories) for 20 min ...
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bioRxiv - Neuroscience 2021Quote: ... followed by an incubation (3 days) with secondary antibodies: donkey anti-chicken Alexa 488 (1:500, Jackson ImmunoResearch Laboratories, Inc, PA, USA) and donkey anti-rabbit Dylight 650 (1:500 ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed 3 times TBST and then incubated with donkey anti-rabbit or donkey anti-mouse HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories) for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were washed in PBS and were incubated in blocking solution [PBS supplemented with 3% donkey serum (Jackson ImmunoResearch, 017-000-121) and 0.1% Triton-X] for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Sections were then washed 3 times in PBS and incubated with Alexa Fluor®594 Goat Anti-Rabbit IgG at 1:200 (Jackson Immunoresearch) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2020Quote: Slices were washed 3 times with PBS before they were blocked with 10% Normal Donkey Serum (NDS, Jackson ImmunoResearch, 017-000-121) and 0.4% Triton-X in 1X PBS for one hour at RT ...
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bioRxiv - Physiology 2021Quote: ... followed by a secondary donkey anti-rabbit Ig antibody coupled to Alexa 488 (3 μg/ml, Jackson ImmunoResearch Laboratories, West Grove, PA). A goat anti-Aqp2 antibody (0.2 μg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... slices were washed in PBS solution and incubated for 1hr at room temperature with secondary antibody (1:500 Cy™3 AffiniPure Donkey Anti-Goat IgG, Jackson ImmunoResearch, 705-165-147 ...
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bioRxiv - Neuroscience 2020Quote: Slices were washed 3 times with PBS before they were blocked with 10% Normal Donkey Serum (NDS, Jackson ImmunoResearch, 017-000-121) and 0.4% Triton-X in 1X PBS for one hour at RT ...
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bioRxiv - Neuroscience 2022Quote: ... The day after sections were washed 3 times with PBS and incubated with secondary antibodies (Goat anti-rabbit Cy2, 1:200, Jackson Immunoresearch (JI)/111-225-144 ...
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bioRxiv - Neuroscience 2023Quote: ... Free-floating sections were permeabilized and blocked in 0.3% Triton X-100 and 3% normal donkey serum (Jackson ImmunoResearch, West Grove, PA) in PBS for 2 h at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... cultures were blocked in PBS-3% BSA and subjected to immunofluorescence labeling using primary antibodies followed by incubation with appropriate secondary antibodies conjugated to Alexa-fluor 488 or Cy-3 (Jackson Immunoresearch Laboratories), and processed for DAPI or Hoechst staining to visualize cells’ nuclei before mounting in ImmuMount medium (Shandon) ...
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bioRxiv - Developmental Biology 2024Quote: ... Secondary antibodies were diluted in block and used at 3-4ug/ml for 1 hour at room temperature (Alexa488, Cy3, orAlexa647; Molecular Probes and Jackson ImmunoResearch). Hoechst at 0.2 ug/ml was used to stain DNA for 3 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Thermo Fisher)] in PBST + 3% BSA for 1 hr followed by either horseradish peroxidase-conjugated polyclonal goat anti-mouse (115–035-146; Jackson ImmunoResearch Laboratories) or horseradish peroxidase-conjugated polyclonal goat anti-rabbit (170-6515 ...
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bioRxiv - Neuroscience 2024Quote: ... were incubated ON at 4°C followed by 3 washes with PBS and the corresponding secondary antibodies (1:500; Jackson ImmunoResearch Laboratories) containing 4′,6-diamidino-2-phenylindol (DAPI ...
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bioRxiv - Neuroscience 2024Quote: ... were incubated in blocking solution (3% normal goat serum [#S-1000; Vector Laboratories] or normal donkey serum [#017-000-121; Jackson ImmunoResearch]) for 2 hours at room temperature and then incubated for 48 hours at 4°C with primary antibodies ...
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bioRxiv - Physiology 2024Quote: ... slices were incubated at 4°C for 48–72 h in pSTAT3 antibodies (1:3000) in 3% normal donkey serum (Jackson ImmunoResearch Labs) with PBT and 0.02% sodium azide ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed in 1X PBS then transferred to secondary antibody (Jackson ImmunoResearch Labs: 1:500 in 3% NDS in 1X PBS) overnight at 4C ...
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bioRxiv - Immunology 2021Quote: ... Bound antibodies were detected with 50 μl of 1 μg/ml alkaline phosphatase-conjugated anti-human IgA and horseradish peroxidase-conjugated anti-human IgG (diluted in PBS containing 0.1% Tween 20 and 1% BSA) antibody (Jackson Immunoresearch, West Grove, PA) for 2 hours at 37°C in 5% CO2 and then developed with VECTOR Blue ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse-on-mouse interference was reduced using Affinpure donkey polyclonal anti-mouse IgG Fab fragments at a 1:20 concentration (715-007-003; Jackson ImmunoResearch Laboratories Inc) for two hours preceding incubation with the unconjugated primary antibodies targeting dsRNA and SARS-CoV-2 spike as described above.
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bioRxiv - Cell Biology 2024Quote: ... 0.05% Tween 20 and 0.05% sodium azide in 20 mM Tris-HCl (pH 8.0) and incubated with secondary antibodies conjugated with 18-nm colloidal gold (Jackson ImmunoResearch; 111-215-144) diluted (1:10 ...
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bioRxiv - Developmental Biology 2024Quote: ... The following secondary antibodies were used at 1:5000 in 5% milk in 1x TBS with 0.1% Tween-20: Peroxidase-AffiniPure Goat Anti-Rabbit IgG (H+L) (RRID: AB_2313567, Jackson ImmunoResearch Laboratories, West Grove, PA), and Peroxidase-AffiniPure Goat Anti-Rat IgG (H+L ...
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bioRxiv - Cell Biology 2020Quote: ... The blockage of nonspecific sites was performed by 1% BSA and 5 μg/mL donkey-IgG (Jackson ImmunoResearch) in PBS for 45min ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated for 15 min on ice with 5 μg/ml human polyclonal IgG (ChromPure, Jackson ImmunoResearch). Unbound IgGs were removed by two washes in cold PBS ...
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bioRxiv - Immunology 2021Quote: Cells were incubated for 15 min on ice with 5 μg/ml human polyclonal IgG (ChromPure, Jackson ImmunoResearch) and the excess of unbound IgGs was removed by two washes in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... neurons were permeabilized and blocked simultaneously in PBS containing 0.1% Triton and 5% normal goat serum (Jackson Immunoresearch) for 1 h at room temperature followed by incubation with primary antibodies overnight at 4°C ...
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... Cyanine Cy™5-conjugated donkey anti-rabbit IgG (Jackson ImmunoResearch Laboratories, 711-175-152; IF 1:400) and Cyanine Cy™5-conjugated donkey anti-goat IgG (Jackson ImmunoResearch Laboratories ...