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Citations for Jackson ImmunoResearch :
501 - 550 of 668 citations for 3 hydroxy 20 oxopregn 5 en 21 yl acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Cells were blocked and permeabilized with in a buffer containing 5% normal donkey serum (Jackson ImmunoResearch, 017-000-121), 1% non-fat milk ...
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bioRxiv - Bioengineering 2024Quote: ... the tissue sections were blocked and permeabilized using 1X TBS containing 5% Normal donkey serum (NDS, Jackson ImmunoResearch Laboratories) and 0.5% Triton x-100 for one hour ...
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bioRxiv - Cell Biology 2024Quote: ... eBioscience) that was pre-incubated with 5 µg/ml goat anti-rat secondary antibody (112-005-003, Jackson ImmunoResearch) for 15 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Primary antibody against DPF-3 was used at 1:200 dilution and anti-rat secondary antibodies fused to HRP (Jackson ImmunoResearch, 712-035-150) were used at 1:2000 dilution ...
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bioRxiv - Physiology 2020Quote: ... Cells were then rinsed 3 times with PBS and incubated with FITC-Goat Anti-Mouse IgG (Jackson ImmunoResearch Inc.,115-095-003; 1:200) with tetramethylrhodamine (TRITC)-phalloidin (1 μg/mL ...
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bioRxiv - Bioengineering 2021Quote: ... the embryos were washed 3 times in the staining buffer (30 mins intervals between washes) and labeled with Alexa Fluor 488 Streptavidin (Jackson ImmunoResearch, 016-540-084). Labeled embryos were washed 3 times in the staining buffer (30 minutes between washes ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for 45-60 min in blocking solution that consisted of PBS containing 3% Bovine Serum Albumin (Jackson Immunoresearch, 001-000-162) and 3% Donkey Serum (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2021Quote: ... primary staining was followed by Cyanine 3-labeled donkey anti-sheep and a fluorescein isothiocyanate (FITC)-conjugated antibody against either rabbit or mouse antibody (Jackson ImmunoResearch, West Grove, PA). Otherwise ...
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bioRxiv - Developmental Biology 2021Quote: ... The chips were then washed twice with PBS, and the cells were permeabilized with blocking solution (PBS 0.1% Tryton-X, 3% of Donkey Serum (Jackson Immunoresearch Cat#017-000-121)) for 45 minutes at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Retinae were then washed in 0.1% PBST for 3 × 10 minutes at room temperature and incubated with goat-anti mouse Cy3 (1:250, Jackson ImmunoResearch Labs, 115-165-166) or goat-anti rabbit Alexa 647 (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were first washed in PBS 5mins for 3 times and then incubated in blocking solution containing 10% normal donkey serum (017-000-121, Jackson ImmunoResearch, West Grove, PA), 1% bovine serum albumen (A2153 ...
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bioRxiv - Systems Biology 2023Quote: The following secondary antibodies were used for staining: Cy™3 AffiniPure Fab Fragment Goat Anti-Mouse IgG2a (Jackson ImmunoResearch, Cat# 115-167-186), Cy™3 AffiniPure Donkey Anti-Rabbit IgG (H+L ...
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bioRxiv - Molecular Biology 2022Quote: ... Primary antibody was washed in PBS with 0.1% Tween 20 (PBT) before applying a horseradish peroxidase (HRP) conjugated anti-guinea pig antibody (Jackson ImmunoResearch Laboratories, INC., West Grove, PA) at a final concentration of 1:250 for 2 hrs at RT or ON at at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Slides were washed three times with TBS buffer with 0,05 % Tween-20 and further incubated with anti-rabbit Alexa 647 (Jackson ImmunoResearch Cat. No. 111-606-144), anti-human IgG-Alexa647 (Southern Biotech ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were stained with Th antibody (Santa Cruz Biotechnology, #sc-25269; 1/20 dilution; anti-mouse IgG2a secondary Fab fragment conjugated with Alexa 594 (Jackson ImmunoResearch, #115-587-186)) and nuclear staining (SYTOX-G) ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were washed thrice with TBS containing 0.1% Tween-20 followed by incubation with goat anti-mouse IgG HRP-conjugate (Jackson ImmunoResearch, 115-035-003; 1:5000). Membranes were washed thrice with TBS containing 0.1% Tween-20 ...
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bioRxiv - Immunology 2024Quote: ... Grids were washed in PBS and incubated with a 12 nm Colloidal Gold AffiniPure Goat Anti-Mouse IgG (1:20 dilution; Jackson ImmunoResearch Laboratories, West Grove, PA), washed in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... then probed with H2.3 primary rabbit polyclonal anti-HSulf-2 antibody (dil. 1/1000) followed by fluorescent-conjugated secondary anti-rabbit antibody (Jackson ImmunoResearch Laboratories, dil. 1/500). All the incubations were performed for 2 h at 4 °C in TBS ...
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bioRxiv - Cell Biology 2022Quote: ... After three washes with PBS the sample was incubated for 3-4h with a secondary donkey anti-rabbit antibody (711-005-152; Jackson ImmunoResearch, West Grove, PA, USA) that was conjugated to Alexa Fluor 647-NHS at an average degree of labeling of 1.5 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed and then incubated 3 h with a secondary antibodies cocktail of Cy3 donkey anti-rabbit (1:600; Jackson ImmunoResearch; #711-165-152, AB_2307443) and Cy2 donkey anti-chicken (1:400 ...
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bioRxiv - Neuroscience 2024Quote: ... Clone SMI-31 reacts with a phosphorylated epitope of neurofilament in mammals and neurofilament 220 in squid51–53 .The secondary Alexa Fluor® 488 AffiniPure Goat Anti-Mouse IgG (Cat#: 115-545-003) and Cy™3 AffiniPure Donkey Anti-Mouse IgG (Cat#: 715-165-151) were purchased from Jackson ImmunoResearch (West Grove, PA) and employed at 1:500 dilutions.
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bioRxiv - Cell Biology 2020Quote: ... The membrane was rinsed 5 x with TBST and incubated in TBST with goat secondary antibodies coupled to HRP (1:10’000; Jackson ImmunoResearch) for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissue was circled with a pap pen and after washing three times with PBS was blocked in in PBS with 0.3% Triton X-100 and 5% normal donkey serum (017-000-121, Jackson ImmunoResearch). After blocking ...
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bioRxiv - Microbiology 2021Quote: ... After fixation the cells were permeabilized with 0.5% Triton X-100 in PBS and blocked in 5% (v/v) normal donkey serum (Jackson Immunoresearch). Cells were incubated with primary antibodies at 37°C for 1 hour ...
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bioRxiv - Bioengineering 2020Quote: ... and resuspension for FACS was performed in a FACS buffer solution containing 5% goat serum (Jackson Immunoresearch #005-000-121) and 1 mM EDTA in 1× phosphate buffer saline (PBS) ...
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bioRxiv - Neuroscience 2020Quote: ... Coverslips were incubated in blocking solution (10% IgG-Free BSA, 5% donkey serum or goat serum in PBS, Jackson ImmunoResearch) for 30 minutes at room temperature (RT) ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was rinsed 5 x with TBST and incubated in TBST/5 % dry milk with goat secondary antibodies coupled to HRP (1:10’000; Jackson ImmunoResearch) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... plates were incubated with peroxidase conjugated goat anti-human IgG secondary (1/10,000 in 5% non-fat milk in PBS; Jackson ImmunoResearch) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... followed by three washes in PBS for 5 min and incubation with secondary antibodies (fluorophore-coupled donkey antibodies, Jackson ImmunoResearch) in blocking solution at RT for 30 to 60 min ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies were then crosslinked with donkey anti-mouse IgG (5 μg/ml, 715-005-150, Jackson Immunoresearch, West Grove, PA) on ice for another 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... Sections were permeabilized with 0.5% Triton X-100/PBS and then blocked with 5% goat serum (Jackson ImmunoResearch Laboratories, USA) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... Non-specific immunoreactivity was suppressed by incubating the sections in a mixture of 5% normal donkey serum (NDS; Jackson ImmunoResearch) and 0.3% Triton X-100 (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... (5) cultures were incubated in Cy3-AffiniPure Donkey Anti-Rabbit IgG (H+L) (1:500, Jackson ImmunoResearch, 711-165-152) and Alexa Fluor 488 AffiniPure Donkey Anti-Chicken IgG (H+L ...
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bioRxiv - Immunology 2022Quote: ... incubated in 0.1 M glycine for 10 min at room temperature and subsequently incubated in 5% goat serum (Jackson ImmunoResearch) for 20 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... the slides were washed briefly with PBS followed by permeabilization and blocking with 0.3% TritonX-100 and 5% Normal Donkey Serum (NDS, Jackson ImmunoResearch) in PBS for 1 hour at RT ...
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bioRxiv - Neuroscience 2021Quote: ... and then blocked for 1 hour at room temperature in 5% normal donkey serum (Jackson ImmunoResearch, West Grove, PA, USA)/0.5% TTBS ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were blocked for 2 hours at room temperature (RT) in 5% normal goat serum (Jackson ImmunoResearch, West Grove, PA). Cells were incubated in one of the following primary antibodies in blocking solution containing 5% normal serum at 4°C overnight ...
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bioRxiv - Neuroscience 2024Quote: Cells were fixed in 4% PFA, washed in PBS and blocked in PBTA (PBS, 5% normal donkey serum (Jackson ImmunoResearch), 0.3% Triton X-100 ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 µm cryosections were blocked in PBS with 0.3% Triton X-100 and 5% normal donkey serum (017-000-121, Jackson ImmunoResearch) and incubated overnight in a humidified chamber at 4 °C with primary antibodies diluted in PBS with 0.3% Triton X-100 and raised against ...
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bioRxiv - Immunology 2023Quote: ... Sections were washed in PBS and blocked in PBS containing 0.1% Triton X-100 and 5% normal donkey serum (NDS) (Jackson ImmunoResearch) for 45 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... and blocked with Image-iT FX Signal Enhancer (Cell Signal) followed by PBS containing 0.1% (v/v) Tween20 and 5% (v/v) normal goat serum (Jackson Immunoresearch). Samples were then stained overnight at 4C with anti-CD4 (AlexaFluor488 ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated with blocking buffer (5% normal donkey serum [Jackson ImmunoResearch] in 0.1% Triton X-100 in PBS [PBS-Tx]) for 1 hour at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... frozen sections at 10-μm thickness were blocked in PBS with 0.3% Triton X-100 and 5% normal donkey serum (017-000-121, Jackson ImmunoResearch) and then incubated with primary antibodies diluted in PBS with 0.3% Triton X-100 at 4°C overnight in a humidified chamber ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were incubated in blocking solution containing 5% normal donkey serum (NDS) (Jackson Immunoresearch, West Grove, PA, USA; Cat#NC9624464) and 0.2% Triton-X 100 in 1x PBS for 1 hour and then incubated in primary antibodies with 2.5% NDS and 0.1% Triton-X 100 in 1x PBS either for 2 hours at room temperature (RT ...
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bioRxiv - Immunology 2024Quote: ... and specified combinations of 5 µg/mL (unless otherwise stated) goat anti-mouse IgM Fab2 (Jackson Immunoresearch 115-006-075), 1 µg/mL anti-CD40 (bioxcell BP0016-2) ...
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bioRxiv - Immunology 2024Quote: ... Fixed and permeabilized cells were then washed 3x with PBS, and blocked (PBS, 0.3% Triton X-100, and 5% normal human serum (Jackson ImmunoResearch)) for 1h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... cells were then permeabilised with 0.1% Triton X-100 for 10 minutes and then blocked in 5% goat serum (diluted in 0.01M PBS: block, Jackson ImmunoResearch) for 1 hr ...
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bioRxiv - Neuroscience 2024Quote: ... followed by blocking for 1□h with 0.2% Triton X-100 and 5% normal goat serum (Jackson ImmunoResearch, Suffolk, UK) in PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were washed with PBS three times at room temperature and were blocked in PBS 0.3% Triton X-100 (PBST) with 5% donkey serum (017-000-121, Jackson ImmunoResearch). Primary antibodies were diluted in PBST and incubated overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Immunohistochemistry for dsRed was performed with a 7 minute −20°C 100% acetone target retrieval and blocking in 2% normal goat serum (Jackson ImmunoResearch, cat. no. 005-000-121). Primary antibodies include rabbit anti-mCherry (1:100 ...