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Citations for Jackson ImmunoResearch :
451 - 500 of 668 citations for 3 hydroxy 20 oxopregn 5 en 21 yl acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... brains were blocked for 30 min in PBS supplemented with 5% normal donkey serum (Jackson Immunoresearch (PA, USA)) and 0.3% Triton X-100 (Fisher) ...
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bioRxiv - Neuroscience 2021Quote: ... sections were blocked with 5% normal donkey serum (cat# 017-000-121, Jackson ImmunoResearch, West Grove, PA, USA) for 1 hr ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed in PBS before blocking in 5% normal goat serum (Jackson ImmunoResearch 005-000-121; NGS) with 0.05% Triton X-100 (Sigma #T9284100 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then blocked with 5% NGS/PBS and incubated with rhodamine (TRITC) streptavidin (1:400, Jackson ImmunoResearch Labs Inc. ...
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bioRxiv - Cancer Biology 2024Quote: ... fixed cells were incubated with PBS solution containing 5% donkey serum (Jackson ImmunoResearch Labs, West Grove, PA, USA) and 0.2% Triton X-100 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Cy™5 AffiniPure Donkey Anti-Guinea Pig IgG (H+L) (706-175-148) were from Jackson ImmunoResearch Laboratories ...
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bioRxiv - Physiology 2023Quote: ... Un-specific binding was blocked with 5% bovine serum albumin and 10% donkey serum (Jackson ImmunoResearch, 017-000121) in 1X PBS for 60 min ...
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bioRxiv - Cancer Biology 2023Quote: ... The sections were subsequently incubated in the blocking buffer of 5% normal donkey serum (Jackson ImmunoResearch, Cat: 017000121) or 10% normal goat serum (Life Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated in blocking solution (5% normal donkey serum in 0.3% PBST; 017-000-001, Jackson ImmunoResearch Laboratories) for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated in blocking solution (5% normal donkey serum in 0.3% PBST; 017-000-001, Jackson ImmunoResearch Laboratories) for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... slides were washed 3× in PBST for 10min each and stained with secondary antibody Goat α-Rabbit-Cy3 (Jackson ImmunoResearch, West Grove, PA) 1:500 in 5% normal goat serum PBST for 2h at RT and then rinsed 3× ...
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bioRxiv - Bioengineering 2020Quote: ... Total mouse IgG against GAA was measured using HRP-goat anti-mouse IgG (subclasses 1 + 2a + 2b + 3) (Jackson Immunoresearch, 115-035-164) and a colorimetric kit (BD Biosciences ...
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bioRxiv - Neuroscience 2021Quote: ... samples were incubated with secondary antibody (PTwH, 3% donkey serum, Cy3-conjugated Donkey Anti-Goat, Jackson ImmunoResearch Labs 705-165-147, 1:1000) overnight ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated for 3 hours at 22-25 °C with Cy3-conjugated anti-mouse and secondary antibodies (Jackson ImmunoResearch, 715-165-150) at 1:500 in a solution of 1.5% BSA in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated for 3 hours at 22-25 °C with Cy3-conjugated anti-mouse and secondary antibodies (Jackson ImmunoResearch, 715-165-150) at 1:500 in a solution of 1.5% BSA in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... The slices were rinsed with PBS (3 × 10 min) and incubated with a secondary antibody solution (1:1000, 711-175-152, Jackson Immunoresearch, Ely, UK)) containing 3 % NDS in PBS for 1.5 h at RT.
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bioRxiv - Neuroscience 2022Quote: ... DRN sections were rinsed in PBS (3 × 10 min) and incubated with a secondary antibody solution (donkey anti rabbit Cy5 (1:1000, 711-175-152, Jackson Immunoresearch, Ely, UK) and goat anti mouse Alexa 555 (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... This was followed by further rinses in 0.01 M PBS (3 × 10min) and a 12h incubation at 4 °C in biotin-streptavidin conjugated AffiniPure IgG (anti-rabbit-streptavidin Jackson Immunoresearch, 1:200), followed by three washes with 0.01 M PBS ...
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bioRxiv - Immunology 2023Quote: ... Endogenous peroxidase activity was blocked with 3% hydrogen peroxide and sections were blocked with 2% BSA (Jackson ImmunoResearch Lab, Cat# 001-000-162) for 1h at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... Membranes were then washed with TBST (3 times for 15 min) and incubated with anti-rabbit-HRP (111-036-045; Jackson ImmunoResearch; 1:5000) secondary antibody ...
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bioRxiv - Genomics 2023Quote: ... 3 times at room temperature and then incubated with secondary antibodies: Cy3 donkey anti-goat (Jackson ImmunoResearch, Cat# 715-165-150, 1:500), Alexa Fluor 488 donkey anti-mouse (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3 times for 10 minutes in PBS before incubation with the secondary antibody (1:1000, donkey anti-rabbit IgG, Jackson ImmunoResearch, PA, USA) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... neurons were incubated with Cy™3 AffiniPure Donkey Anti-Rabbit IgG (H+L) (1.9 µg/ml; Jackson ImmunoResearch, cat #111-165-003) for standard epifluorescence assays ...
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bioRxiv - Physiology 2023Quote: ... incubated for 90 min at room temperature with a donkey anti-human CyTM 3 antibody (1:500; Jackson ImmunoResearch, Cat #709-165-149) and coverslipped ...
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bioRxiv - Microbiology 2024Quote: ... membranes were washed 3 times with PBS-T before incubation with HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, 115-035-146; 1:5000) for 1 h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... membranes were washed 3 times with PBS-T before incubation with HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, 115-035-146; 1:5000) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were washed (3 × 5mins in 0.01M PBS) and then incubated with secondary antibody for 2 hr (Jackson ImmunoResearch, 1:200 in block). Following washing (3 × 5mins in 0.01M PBS) ...
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bioRxiv - Cell Biology 2020Quote: ... Blots were washed 3X with TBS containing 0.1% Tween-20 and incubated for 1 hr RT with secondary antibody conjugated to horseradish peroxidase diluted into blocking buffer (Jackson ImmunoResearch—West Grove, PA). Blots were washed a final 3X and imaged using Clarity Western ECL substrate (BioRad ...
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bioRxiv - Developmental Biology 2023Quote: ... The grids were incubated with 6 nm Colloidal Gold AffiniPure Goat Anti-Mouse IgG (H+L) (Jackson ImmunoResearch, 115-195-146, 1:20, AB_2338728) for 30 min at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary anti-neurofilament antibody (DHSB, 2H3) was used at a 1:20 dilution and anti-mouse HRP-conjugated secondary antibody (Jackson ImmunoResearch, 115-035-003) was used at 1:1000 dilution ...
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bioRxiv - Immunology 2024Quote: ... After washing twice with 200 μl FACS buffer the cells were incubated in 30 μl of FACS buffer for 20 min with 1:1000 diluted APC-conjugated F(ab’)₂ fragment goat anti-human IgG (Jackson ImmunoResearch, West Grove, USA). Following two further washes with FACS buffer the cells were resuspended in 100 μl FACS buffer with 0.1 µg/ml propidium iodide (BD Pharmingen) ...
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bioRxiv - Cell Biology 2020Quote: ... wherein fixed CDMs and/or cells were blocked in 5% normal donkey serum (017-000-121, Jackson Immunoresearch Labs) and 0.3% Triton-X 100 (T8787 ...
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bioRxiv - Developmental Biology 2021Quote: ... After sequential blocking for each 1 h in 5% donkey and fetal calf serum (Jackson Immunoresearch, Ely, United Kingdom) and 0.5% Triton X-100 (Merck) ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated for 5 mins with 2% (vol/vol) normal mouse and normal rat serum (Jackson ImmunoResearch Laboratories), prior to staining with a 2X master mix containing fluorophore-labeled antibodies at pre-titrated concentrations in Brilliant Violet Staining Buffer (BD Biosciences) ...
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bioRxiv - Neuroscience 2021Quote: ... permeabilized with PBS containing 0.3% Triton X-100 (PBS-T) and blocked with 5% normal donkey serum (Jackson ImmunoResearch). The tissues sections were then incubated with primary antibody (rabbit anti-c-Fos ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections were washed in PBS and blocked for 1 hour in 5% normal donkey serum (NDS, Jackson Immunoresearch) and 0.3% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... and blocked for 45 min in block buffer (0.05 % NP40, 0.05 % Triton-X 100 and 5 % goat serum (Jackson ImmunoResearch) in PBS) ...
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... and Cyanine Cy™5-conjugated donkey anti-goat IgG (Jackson ImmunoResearch Laboratories, 705-175-147; IF 1:400).
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bioRxiv - Immunology 2020Quote: ... 2% heat-inactivated FBS (Omega Scientific)] and blocked with blocking buffer [FACS buffer with 5% normal Syrian hamster serum (Jackson Immunoresearch, cat#007-000-120), 5% normal mouse serum (Jackson Immunoresearch ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated in blocking solution including TBS + 0.3% Triton X-100 (0.3% TBST) + 5% Donkey Normal Serum (DNS) + IgG Fab blocking reagent (90uL/ml; Jackson Immunoresearch) for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... the brains were blocked in 5% normal goat serum solution (NGS; 005-000-121, Jackson Immunoresearch Laboratories; in PBS) for 2 h at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... (Yin et al. 1997)) and then by goat-anti-rabbit IgG-HRP (1:5000 in 5% milk, Jackson ImmunoResearch). HRP signal was detected using EZ-ECL kit (Biological Industries) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were blocked for 1 h in blocking buffer (0.05% NP40, 0.05% Triton-X 100 and 5% goat serum (Jackson ImmunoResearch) in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Blocking buffer consisted of 5% heat-shocked BSA in Tris-buffered saline and 10% normal donkey serum (Jackson Immunoresearch). Immunofluorescence buffer was comprised of 2% heat-shocked BSA in Tris-buffered saline ...
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bioRxiv - Immunology 2023Quote: ... washed in distilled water and blocked for 30 minutes with 5% normal goat serum (Jackson ImmunoResearch 005-000-121) in PBS-T (PBS (VWR 45000-436) ...
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bioRxiv - Immunology 2023Quote: ... a secondary stain was performed using 5 ng/ml anti-human IgG-AlexaFluor647 (AF647) (Jackson Immunoresearch, 109-605-098) (1:200 dilution) ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 1.5 h incubation in blocking solution of 0.1 M PBS containing 0.3% Triton X-100 and 5% Normal Donkey Serum (NDS, Jackson Immunoresearch). Sections were then transferred in blocking solution containing primary antibodies and incubated for 1-2 overnights at 4°C ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... permeabilization/wash buffer (0.1% Triton X-100 in 1X PBS) and blocking buffer (5% normal goat serum (Jackson ImmunoResearch), 0.1% Triton X-100 in 1X PBS ...
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bioRxiv - Neuroscience 2022Quote: ... slides were incubated for one hour in blocking buffer containing 5% normal donkey serum (Jackson ImmunoResearch, West Grove, PA) and 0.3% Triton X-100 in PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were cut at 30 um thickness and blocked in 5% normal donkey serum (017-000-121, Jackson ImmunoResearch) in PBS with 0.3% Triton X-100 (PBST ...