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Citations for Jackson ImmunoResearch :
601 - 650 of 668 citations for 3 hydroxy 20 oxopregn 5 en 21 yl acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Foxn3 is part of a transcriptional network that regulates cilia genes in the developing mouse retinabioRxiv - Developmental Biology 2024Quote: Retina sections were treated with a blocking solution (1% unlabeled adinity-purified Fab fragment donkey anti-mouse IgG (H+L) and 5% donkey serum (Jackson Immunoresearch) in PBS containing 0.1% Triton X-100 (ThermoFisher) ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary gold labeling was performed for 1.5 hours at 1:20 dilution in blocking solution (18 nm gold donkey-anti-mouse and donkey-anti-rabbit IgG, Jackson ImmunoResearch 705-215-147, 711-215-152). Post-staining ...
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bioRxiv - Immunology 2022Quote: ... The slides were washed with TBS-T (Tris-Buffered Saline with Tween 20) and incubated with secondary antibodies alpaca anti-mouse IgG Alexa Fluor 594 (Jackson ImmunoResearch; cat. 615-585-214; 1:1,000), donkey anti-goat IgG Alexa Fluor 488 (Abcam ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then washed 3 times in 0,05% Tween 20-PBS and stained with an appropriate secondary antibody conjugated to an Alexa Fluor (AF) dye (Jackson Immunoresearch 715-545-150; 711-545-152 (AF488); 715-165-150 (Cy3) ...
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bioRxiv - Biochemistry 2020Quote: ... The α-actin secondary antibody was a Cy-3 conjugated AffiniPure donkey anti-mouse at a dilution of 1:500 (Jackson ImmunoResearch Laboratories Inc. #715-166-150). Bands for p-ERK ...
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bioRxiv - Neuroscience 2020Quote: ... selected coronal sections were rinsed three times in TBS and incubated in blocking solution (3% normal donkey serum, Jackson Immunoresearch AB2337258; 0.3% Triton X-100 in TBS) at room temperature for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were incubated for 45 min with the secondary antibody Cy™3 AffiniPure F(ab’)2 Fragment Goat Anti-Mouse IgG (H+L) (Jackson ImmunoResearch Laboratories Inc., 1:200 dilution). DAPI (0.2 mg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were incubated with primary antibodies (see Key Resources Table) overnight and subsequent incubated with secondary antibodies labeled with Alexa Fluor 488- (Jackson ImmunoResearch, 115-545-003, 3 μg/mL) or Cy3- (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2024Quote: Cav2sfGFP (Gratz et al. 2019) localization (Figs. 2 and 3): Primary antibodies (α-brp nc-82, 1:400, DSHB; α-HRP rabbit, 1:500, Jackson Immunoresearch Cat# 323-005-021) were applied in PBS-Tx 0.3 % overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Blots were washed 3 x 5 mins in 1X TBST and incubated in 5% non-fat dry milk in 1X TBST containing anti-rabbit (H+L) horseradish peroxidase (Jackson ImmunoResearch Laboratories) secondary antibodies at a dilution of 1:20,000 for 1 hour at room temp ...
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bioRxiv - Cell Biology 2020Quote: ... Fc receptors were blocked by incubating cells in PBS supplemented with 5% FCS with 250 µ g.ml−1 purified mouse IgG (Jackson ImmunoResearch Laboratories). Antibody stainings were performed in PBS/5% FCS on ice for 30 minutes with optimal dilutions of commercially-prepared antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed 3 times for 5 minutes each with PBT (0.5% Triton X-100 in PBS) and incubated with the corresponding secondary antibodies Alexa Fluor 488 (Anti-Chicken IgG, Jackson ImmunoResearch Labs) and Cy3 (Anti-Rabbit IgG ...
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bioRxiv - Immunology 2020Quote: ... CAR detection was performed using anti-Fab specific antibody at day 5 post-transduction (109-606-006, Jackson ImmunoResearch, West Grove, PA).
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bioRxiv - Immunology 2021Quote: ... Plated peptides were incubated with the individual monoclonal antibodies (5 µg/ml diluted in blocking buffer) and further incubated with donkey anti-human alkaline phosphatase-conjugated secondary antibody (Jackson ImmunoResearch, USA). Immune complexes were identified following incubation with SIGMAFAST™ PNPP (SIGMA ...
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bioRxiv - Cell Biology 2021Quote: ... membrane was incubated with secondary Goat anti-Chicken HRP (1:5000 in 5%BSA/1X PBS-T, Jackson ImmunoResearch, 103-035-155) then incubated for 2 min in the dark with ECL Western Blotting Substrate (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... it was incubated for 30 min in PBS with 5% non-fat milk and then for 1h at RT with horseradish peroxidase-conjugated secondary anti-mouse antibody (1:10000 dilution in PBS with 5% non-fat milk, Jackson Immunoresearch Laboratories), and washed again ...
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bioRxiv - Physiology 2023Quote: ... slices were washed 3×5 min in PBS 0.01M at RT – 4 mL/well and incubated in donkey anti-guinea pig-AF647 (Jackson ImmunoResearch, 1:500) diluted in [PBS 0.01M + 0.3% Triton] ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were subsequently washed four times for 5 minutes in TBS-T before secondary antibody (Jackson ImmunoResearch, 711-035-152, 1:15,000) was added for 1 hour at room temperature in 5% skim milk ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were permeabilized and blocked for 1–2 h at room temperature (RT) in 1X PBS with 0.5% Triton-X (0.5% PBST) and 5–10% normal donkey serum (NDS, Jackson ImmunoResearch 017-000-121). Primary and secondary antibodies were diluted in 0.5% PBST and 5% NDS ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed 3X using TBST-High Salt for 5 minutes on a rocking surface at room temperature before being incubated with secondary antibodies conjugated with Horseradish Peroxidase (Jackson ImmunoResearch Labs) for 45 minutes in 5% Non-fat dry milk rocking at room temperature ...
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bioRxiv - Immunology 2023Quote: ... before cultured in complete RPMI-1640 media without/with 5 μg/mL goat anti-mouse IgM (Jackson ImmunoResearch; cat # 115-006-075) and/or recombinant murine 25 ng/ml IL-4 (Peprotech ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were permeabilized and blocked in a single step with 0.2% Triton-X and 5% donkey serum (Jackson Immunoresearch, 017-000-121). Primary antibodies were subsequently added and incubated overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Slide regions were washed in PBS for 5 mins and then blocked with 10% normal goat (Jackson ImmunoResearch, West Grove, PA, USA) or donkey serum (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: Samples were washed twice for 10 minutes with PBS-T (0.5% Triton-X100 in PBS) and incubated with Cy3 and/or Alexa Fluor 647-conjugated secondary antibody (1:500, Jackson ImmunoResearch Laboratories), DAPI (4’,6-Diamidino-2-Phenylindole ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated for 3 hours at 22-25 °C with Cy3-conjugated anti-mouse and Alexa488-conjugated anti-chicken secondary antibodies (Jackson ImmunoResearch, 715-165-150 and 711-225-152) at 1:500 and 1:1000 dilution in a solution of 1.5% BSA in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were washed 3 times with PBS and then incubated with 1:200 Alexa Fluor 488 donkey anti-mouse IgG (H+L) (Jackson ImmunoResearch, West Grove, PA, USA; 715-545-151), 1:200 Fluorescein (Cy3 ...
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bioRxiv - Genetics 2021Quote: ... The fixed tissues were then subjected to three rounds of 15 minute-each wash with 0.1% PBST (0.1% Triton X-100 in PBS) and blocked with 5% donkey serum (Jackson ImmunoResearch Laboratories, 107-000-121) for 1 hour at room temperature followed by incubation with primary antibodies in 5% donkey serum at 4°C ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated for 1h at RT with blocking solution of 5% goat serum in PBS (Jackson ImmunoResearch, catalog no. 005–000-121). A rabbit polyclonal anti-SARS-CoV-2 nucleocapsid antibody (GeneTex ...
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bioRxiv - Neuroscience 2021Quote: ... The membranes were blocked in 5% w/v bovine serum albumin (IgG-free and protease-free BSA, Jackson ImmunoResearch Laboratories, West Grove, PA) in 50 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were washed 3 times for 5 minutes each in PBS+0.3% TritonX-100 and incubated with appropriate fluorescent conjugated secondary antibody (Jackson ImmunoResearch, West Grove, PA) for 45 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Antibodies for O1 and O4 were diluted in N2B27 base medium supplemented with 5% Donkey Serum (v/v) (Jackson ImmunoResearch, 017-000-121) and added to wells for 18 minutes at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... 0.1 M glycine, 0.1% Triton-X, syringe filtered) for 10 min, then blocking buffer (1X PBS, 5% goat serum (Jackson ImmunoResearch; 005-000-121), 2% bovine serum albumin (Sigma-Aldrich ...
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Differential impact of a dyskeratosis congenita mutation in TPP1 on mouse hematopoiesis and germlinebioRxiv - Cell Biology 2021Quote: ... deparaffinized sections were boiled in 0.1 M Sodium Citrate for 10 minutes for antigen retrieval and sections were blocked in suppressor serum (5% goat serum (Jackson ImmunoResearch #005-000-1210), 95% blocking solution (3% BSA and 0.5% Tween in PBS) ...
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bioRxiv - Cancer Biology 2022Quote: ... Coverslips were washed with PBS 1X three times for 5 min at RT and incubated with Donkey anti-mouse IgG Alexa Fluor 488 secondary antibody (1:200; Jackson ImmunoResearch, 715-095-150) and Rhodamine-conjugated Phalloidin (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... permeabilized with 0.1% Triton X-100 in PBS (PBST) for 30 min and blocked with 5% normal donkey serum (Jackson ImmunoResearch Laboratories, #017-000-121) diluted in PBST (blocking solution ...
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bioRxiv - Immunology 2020Quote: ... plates were washed 5 times with 0.05% PBST and then incubated with horseradish peroxidase (HRP)-conjugated anti-mouse IgG (Jackson ImmunoResearch Laboratories 115-035-003), IgG1 (Jackson ImmunoResearch Laboratories 115-035-205) ...
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bioRxiv - Neuroscience 2023Quote: ... were washed in 1X TBS three times and then blocked and permeabilized for one hour in 1X TBS containing 5% Normal donkey serum (NDS, Jackson ImmunoResearch Laboratories, Lot #161590) and 0.5% Triton x-100 ...
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bioRxiv - Developmental Biology 2024Quote: P5-6 retinas were incubated in wash buffer (PBS with 0.1%Triton X100) with gentle rocking for 15 min and then in blocking buffer (wash buffer with 5% normal donkey serum (Jackson ImmunoResearch, 017-000-121)) for 1-2 hrs ...
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bioRxiv - Developmental Biology 2023Quote: ... Secondary antibody incubation was performed in 5% milk for 1 hour at room temperature using 1:5000 for anti-rabbit-HRP (Jackson Immunoresearch, 711-035-152) or anti-mouse-HRP (Jackson Immunoresearch ...
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bioRxiv - Neuroscience 2023Quote: ... the tissue was washed six times in PBS (5 min each) then incubated with a biotinylated goat anti-rabbit antibody (1:500; Jackson ImmunoResearch Laboratories; RRID: AB_2337965) prepared in NDS for 1 hr (RT) ...
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bioRxiv - Microbiology 2022Quote: ... The grids were washed on three drops of PBS before being incubated for 12 min with a 5 nm gold-labelled anti-rabbit secondary antibody (Jackson ImmunoResearch West Grove, PA) at dilutions of 1:10 ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were blocked for 1 hour with block buffer (0.05% IGEPAL CA-630, 0.05% Triton X-100, 5% goat normal serum [Jackson ImmunoResearch; 005-000-121] in 1xPBS). Samples were incubated with primaries/nanobodies diluted in block buffer for 16 hours at 4 °C on a rocker ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were then washed with PBS three times (5 minutes each) and incubated with Alexa Fluor 488 anti-mouse secondary antibody (1:1000, Jackson Immunoresearch #115-545-146) at 4°C overnight ...
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bioRxiv - Bioengineering 2024Quote: ... The cells were washed with 1X DPBS and then incubated in blocking buffer (consisting of 5% (v/v) normal donkey serum (Jackson ImmunoResearch, West Grove, PA) and 1% (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... The following secondary antibodies were used at 1:10,000 dilution and incubated for 1 hour at room temperature in 5% milk w/ TBST: HRP donkey anti-rabbit (Jackson ImmunoResearch #711-035-152) and HRP goat anti-mouse IgG subclass 1-specific (Jackson ImmunoResearch #115-035-205) ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were first washed in PBS three times for 5 min each and then incubated in blocking solution containing 10% normal donkey serum (017-000-121, Jackson ImmunoResearch, West Grove, PA, USA), 1% bovine serum albumen (A2153 ...
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bioRxiv - Microbiology 2020Quote: ... cells were incubated for 1 hour at room temperature with blocking solution of 5% goat serum in PBS (Jackson ImmunoResearch: catalog no. 005–000-121). To stain for SARS-CoV-2 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... treated with glycine (100 nM in PBS) and blocked with 5 % skim milk powder or 10 % normal goat serum (Jackson ImmunoResearch, West Grove, Pennsylvania, USA) and 1 % BSA in PBST for 1 h at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed 4 x 5 min in 0.1 M PB then incubated with a biotinylated secondary antibody (biotinylated donkey anti-mouse, Jackson ImmunoResearch, 715-065-151, 1:500) for 1 hour at RT ...
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bioRxiv - Neuroscience 2022Quote: ... RT) in PBS-T containing 5% NGS at the following concentrations: donkey anti guinea pig DyLight 405 (1:500, Jackson ImmunoResearch, West Grove, PA, USA), goat anti mouse Cy3 (1:500 ...