Labshake search
Citations for Beckman :
1901 - 1935 of 1935 citations for 6H Pyrazolo 3 4 g benzothiazole 2 methyl 8CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... A 12 ml 2 % step Optiprep gradient in LB ranging from 32 % to 10 % was prepared beforehand in Ultra-Clear tubes (Beckman Coulter). Samples were spun for 16 hours at 100000 g at 4 °C ...
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bioRxiv - Microbiology 2024Quote: Intracellular HIV-1 p24 staining was performed on fixed U1 and ACH-2 cells with KC57-FITC (Beckman Coulter, Cat#6604665). After allowing at least 30 minutes for fixation ...
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bioRxiv - Genomics 2024Quote: ... The crude nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI) and loaded into a flow cytometer (Beckman Coulter, MoFlo XDP). Nuclei were purified by flow sorting and washed as described previously [61] ...
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bioRxiv - Neuroscience 2023Quote: ... After PCR the samples were combined into small pool of 2-6 samples and size selection was carried out by adding 0.55x volume of AMPure XP beads (Beckman Coulter, #A68831). The concentrations of purified library pools were quantified with Collibri™ Library Quantification Kit (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... viruses were collected and concentrated by ultracentrifugation (37,000 rpm, 2 h 30, 15 °C; Beckman Type 45 Ti fixed-angle rotor). The virus titer was determined using a plaque assay.
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bioRxiv - Microbiology 2023Quote: ... the DNA monitored on 2% agarose gel was purified by means of AMPure XT beads (Beckman Coulter Genomics, Danvers, MA, USA) and quantified by Qubit (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... and 12-fold diluted in ice-cold SCL and subjected to ultracentrifugation at 100 000 x gav for 2 h in a Ti 70 fixed-angle rotor (Beckman Coulter). The resulting pellets were resuspended in SCL and freshly used for downstream analysis and never subjected to freezing and thawing.
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A laboratory framework for ongoing optimisation of amplification based genomic surveillance programsbioRxiv - Genomics 2023Quote: ... PCR amplicons for pools 1 and 2 were combined for each sample and purified with a 1:1 ratio of AMPureXP beads (Beckman Coulter) and eluted in 30μL of sterile water ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mL reaction buffer containing substrates (25 μM-0.16 mM NADH and 2 mM pyruvate) was added to the cuvette of a spectrophotometer (DU® 700, Beckman Coulter). The reaction was started by adding cell lysate ...
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bioRxiv - Neuroscience 2020Quote: ... Final 15 mL of solution was mixed by inverting the tube 10x and carefully pipetted into 2 ultracentrifuge tubes (Beckman Coulter #344059) onto the isolation buffer cushion (5 mL ...
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bioRxiv - Microbiology 2020Quote: ... were pelleted through a 20% (wt/vol) sucrose cushion at 25,000 rpm for 2 h in an SW41 rotor (Beckman Coulter, Tokyo, Japan). Then ...
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bioRxiv - Cancer Biology 2022Quote: ... The cell pellet was disaggregated and incubated with 2 μL of anti-pan cytokeratin-FITC (Beckman Coulter, Cat. No. IM2356U, Clone J1B3). A separate tube containing cells unstained for surface E-Cadherin was incubated after permeabilization with E-Cadherin antibody- (R&D Cat ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: ... Cells were finally washed in PBS with 0.5% BSA and 2 mM EDTA and analyzed by flow cytometry using a CytoFLEX (Beckman Coulter, Indianapolis, IN) and FlowJo software (BD Biosciences) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Clarified supernatants were layered over 2 ml of a 25% (w/v) sucrose solution in 13.2 ml Thinwall Polypropylene SW41 Ti ultracentrifuge tubes (Beckman-Coulter, Brea, USA) and made up to a total volume of 10 ml with PBS without calcium chloride or magnesium chloride (PBS[-]) ...
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bioRxiv - Molecular Biology 2020Quote: ... was subjected to centrifugation at 100,000g for 2 h in an Optima MAX-XP benchtop ultracentrifuge with a TLA-100.3 rotor (Beckman Coulter, Inc., Brea, CA). Pellets were resuspended in 100 µl PBS and stored at −80 °C.
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bioRxiv - Biochemistry 2019Quote: ... in iso-osmolar RB150/Mg2+ (containing a reduced level (2%) of glycerol) and 80µl were transferred to 7 × 20 mm polycarbonate tubes (Beckman Coulter, Brea CA), overlaid with 80µl of 35% ...
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bioRxiv - Microbiology 2021Quote: ... soil was thoroughly mixed with 20 ml of 0.01 M CaCl2 for 30 min on a Elmi Intelli-Mixer RM-2 (Elmi Tech Ltd, Latvia) and subjected to centrifugation in an Allegra X-22R centrifuge (Beckman Coulter, USA) at 3900 rcf for 20 min and to remove inorganic S (Dedourge et al. ...
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bioRxiv - Microbiology 2023Quote: ... the prepared DNA library was size-selected on a 2% agarose gel (410–470 bp) using the Agencourt SPRI system to reduce mitochondrial contamination (Beckman Coulter, Inc.). After library prep ...
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bioRxiv - Plant Biology 2023Quote: ... DNA extracts were purified using a bead clean up method with a 2:1 ratio of Ampure XP beads (Beckman Coulter, USA) to DNA elution ...
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bioRxiv - Biophysics 2023Quote: ... The virions were concentrated by ultracentrifugation through a 20% (w/v) sucrose cushion (2 h at 28,000 rpm in a SW32 rotor [Beckman Coulter Life Sciences]). The pellet was resuspended in PBS ...
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bioRxiv - Genetics 2023Quote: ... in-house VLPs were produced using the same protocol followed by ultracentrifugation at 24,000 RPM for 2 hours (SW 28 Beckman swinging bucket rotor) to concentrate the VLPs and avoid diluting the HSPCs culture media ...
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bioRxiv - Cancer Biology 2020Quote: ... and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1 E7335S, Index Primers Set 2, E7500S) with Agencourt AMPure XP magnetic beads (Beckman coulter A63881) exactly as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 3 μM or 10 μM from a 2 mM or 10 mM stock solution using a Biomek FX with 384-well head (Beckman Coulter, Indianapolis, IN). The DMSO concentration was kept below or at 0.5% v/v ...
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bioRxiv - Physiology 2022Quote: ... the library preparation step followed the CEL-Seq 2 protocol85: the RT product was digested by ExoI and HinFI and purified using AMpure XP beads (Beckman Coulter™, USA). Then ...
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bioRxiv - Microbiology 2024Quote: ... then the colonies were diluted and inoculated into the Neg MIC EN-2J panel containing β-lactams listed in Table 2 and incubated in MicroScan Walkway40 Plus (Beckman Coulter Inc.). Breakpoint criteria for susceptible (S) ...
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bioRxiv - Molecular Biology 2020Quote: ... bound antibodies were visualized using 100 µL of a 1:100 dilution of goat F(ab’)2 anti-human IgG coupled to phycoerythrin (Beckman Coulter, Brea, CA, USA) at 4°C for 30 min ...
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bioRxiv - Biochemistry 2020Quote: Sedimentation velocity experiments were performed using a ProteomeLab XL-1 analytical ultracentrifuge with the use of an AN60-Ti rotor and Epon-2 channel centerpieces (Beckman Coulter, Brea, CA, USA). Protein samples were diluted with SANS buffer to concentrations yielding an absorbance of 0.5 at 280 nm as measured with an 8454 UV–Vis spectrophotometer (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... The success of the amplifications was checked in a 2% agarose gel and PCR products were subsequently purified using Agencourt AMPure XP magnetic beads kit (Beckman Coulter, Brea, CA, USA), quantified with the QuantiFluor™ dsDNA System (Promega ...
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bioRxiv - Molecular Biology 2019Quote: ... The concentrated urine sample was resuspended in 3.2 ml of a 50% iodixanol solution and layered on the bottom of a 17 ml Thinwall Polypropylene Tube (Beckman Coulter, Fullerton, California, USA). A discontinuous DG was prepared by additional layering of 4 ml of 20% ...
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bioRxiv - Biochemistry 2019Quote: ... and 5 mM MgCl2 at pD 7.5 corresponding to a pH reading of 7.1) at 25°C as follows: spin column at 2000 rpm for 2 min in a tabletop centrifuge (Beckman Coulter Allegra R Tabletop Centrifuge), discard the flow-through ...
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bioRxiv - Microbiology 2023Quote: The amplicons obtained with each of the strategies (using pools 1 and 2) were pooled in each group and purified with one volume of AMPure XP beads (Beckman Coulter, Brea, CA, USA). The beads were washed with 80% ethanol ...
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bioRxiv - Biochemistry 2023Quote: ... cells were treated with the drugs (20 µM to a suspension at 500,000 cells/mL in PBS containing 2 % FBS at 37°C) and directly analyzed by flow cytometry (Gallios, Beckman-Coulter, 405/488 nm laser) over 2 h ...
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bioRxiv - Cancer Biology 2020Quote: ... Filtered media containing lentiviral particles were centrifuged at 24000rpm at 20°C for 2 hrs in 90Ti rotor in ultra-centrifuge (Beckman Coulter Optima TM L-100K). Viral particle pellet was obtained after discarding supernatant post centrifugation and suspended in 100 μl OptiMEM media for overnight at 4°C.The collected lentivirus suspension was used for transfection or stored for later use at −80°C ...
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bioRxiv - Microbiology 2024Quote: ... Viruses were collected 48 hours post-transfection and pelleted in DPBS over a 10% sucrose cushion at 25,000 RPM for 2 hours using a SW32Ti rotor (Beckman Coulter Life Sciences, Brea, CA, USA). Virus pellet was then resuspended in labeling buffer (50 mM HEPES pH 7.0 ...
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bioRxiv - Microbiology 2023Quote: ... another 2 mL of staining bacterial samples was analyzed with a flow cytometry to check cell viability (Beckman CytoFLEX FCM, USA, Channel FL2-A: PE-A).