Labshake search
Citations for Beckman :
1701 - 1750 of 1935 citations for 6H Pyrazolo 3 4 g benzothiazole 2 methyl 8CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2021Quote: ... Syringes were placed inside 50-mL tubes and centrifuged for 20 min at 700g with slow acceleration (4°C, JA-14 rotor, Avanti J-20 XP Centrifuge; Beckman Coulter). The apoplastic wash fluid was then filtered through a 0.22 μm membrane and centrifuged at 10,000g for 30 minutes to remove any remaining large particles ...
-
bioRxiv - Microbiology 2021Quote: ... cells were fixed with 4% Paraformaldehyde for 30 minutes at room temperature and analyzed for GFP fluorescence on a Gallios flow cytometer (Beckman-Coulter). For SARS-CoV-2 experiments ...
-
bioRxiv - Microbiology 2021Quote: ... and cytoplasmic membranes were precipitated by ultracentrifugation for 45 min at 55,000 rpm and 4°C (Beckman Coulter rotor TLA 55). Membrane fractions resuspended in resuspension buffer (750 mM aminocaproic acid ...
-
bioRxiv - Biophysics 2021Quote: ... The crude lysate was sonicated on ice and clarified by centrifugation for 30 min at 4°C and 35,000 rpm in a Beckman L90K Optima and 50.2 Ti rotor (Beckman, Brea, CA, USA). The clarified lysate was applied to a HisTrapHP column (Cytiva ...
-
bioRxiv - Biophysics 2021Quote: ... and the fractions containing the protein of interest were combined and concentrated to 2mL at a final concentration of 3.85 mg/mL by repeated centrifugation in an Amicon Ultra Centrifugal Filter at 4k rpm for 5 min at 4 °C (Beckman SX4250). The concentrated protein was incubated overnight with TEV (1:50 mass ratio ...
-
bioRxiv - Cancer Biology 2022Quote: ... vehicle control or compounds from the small molecule library (Supplementary Table 4) were added using an Echo liquid handler (Labcyte/Beckman Coulter). Cells were then incubated in an IncuCyte Zoom (Sartorius ...
-
bioRxiv - Microbiology 2022Quote: ... Most of the supernatant was discarded leaving 12x∼200 µL of concentrated fibers that were pooled and layered on top of ultracentrifuge tubes of 4 mL (polypropylene centrifuge tubes, Beckman Coulter) containing a discontinuous sucrose gradient (40% ...
-
bioRxiv - Biophysics 2022Quote: ... and the supernatant was subjected to ultracentrifugation to separate the membrane fraction (100,000g, 1 hour, 4°C using an Optima XE-90, Beckman Coulter centrifuge). Membranes were resuspended in lysis buffer supplemented with cOmplete EDTA-free protease inhibitors (Roche) ...
-
bioRxiv - Molecular Biology 2020Quote: ... The filtered supernatant was then centrifuged in JA20 tubes at 6000 rpm overnight (O/N) at 4°C (Avanti J-25, Beckman Coulter). The following day the viral pellet was resuspended in 5 ml PBS and centrifuged at 20,000 rpm for 90 mins at 4°C (Optima XL-100K ...
-
bioRxiv - Molecular Biology 2020Quote: ... The gradients were centrifuged at 40000 rpm for 2h at 4°C in a SW 60 Ti swinging-bucket rotor (Beckman Coulter). Afterwards ...
-
Single cell analysis of the cellular heterogeneity and interactions in the injured mouse spinal cordbioRxiv - Neuroscience 2020Quote: ... were added to each sample well and cells were kept at 4°C until analysis on a Cytoflex Flow Cytometer (Beckman Coulter).
-
bioRxiv - Immunology 2021Quote: ... 4 ng of RNA were retrotranscribed and cDNA was amplified using 13 cycles and purified with AMPure XP beads (Beckman Coulter). Concentration was determined using Qubit 3.0 (Life Technologies ...
-
bioRxiv - Cancer Biology 2020Quote: ... cells were grown for 4-6 days and counted using an Incucyte (Essen Biosciences) or a Z2 Coulter Counter (Beckman Coulter). Small molecule dose-response curves were printed using an D300e Digital Dispenser (HP Specialty Printing Systems ...
-
bioRxiv - Microbiology 2022Quote: ... and the viral particles were concentrated by ultracentrifugation for 1h at 30,000 rpm at 4°C in an SW40 rotor (Beckman, Fullerton, CA). Viral pellets were resuspended in TNC buffer (10 mM Tris-HCl [pH 7.5] ...
-
bioRxiv - Neuroscience 2022Quote: ... dispersed and collected in a single tube and further centrifuged for 4 hours at 21000 rpm in a 20% sucrose gradient (SW55 Beckman rotor). The resulting pellet was suspended in 100 μl PBS and the virus was aliquoted (5-10 μl ...
-
bioRxiv - Systems Biology 2020Quote: ... The resultant supernatant was collected and underwent ultracentrifugation at 100,000xg for 40 minutes at 4°C (rotor MLA-55) in 10.4 ml 16×76 mm polycarbonate capped centrifuge tubes (Beckman Coulter 355603), the pellet was washed once in PBS−/− and centrifuged again at 100,000xg for 40 minutes at 4°C ...
-
bioRxiv - Microbiology 2019Quote: ... The concentrated vesicles were washed twice with PBS by ultracentrifugation at 110,000xg at 4°C for 1 hour (Beckman Optma MAX-XP Ultracentrifuge, rotor TLA 120.2; Beckman Coulter Inc., USA). The final pellet (exosomes ...
-
The antibiotic bedaquiline activates host macrophage innate immune resistance to bacterial infectionbioRxiv - Immunology 2019Quote: ... Cells were detached and were fixed with 4% paraformaldehyde and the fluorescence was analyzed using a CytoFLEX Flow Cytometer (Beckman Coulter).
-
bioRxiv - Cell Biology 2020Quote: ... Reconstituted nucleosome samples were overlaid onto the sucrose gradient and spun at 32000 rpm for 20 h at 4 ° using SW55Ti rotor (Beckman Coulter) in Beckman Optima L80 ...
-
bioRxiv - Molecular Biology 2020Quote: ... the lysate was incubated under rotation for 30 min at 4 °C and cleared for 10 min in an MLA-80 rotor (Beckman Coulter) at 11,500 xg and 4 °C ...
-
bioRxiv - Molecular Biology 2021Quote: ... Indexing PCR amplicons were pooled (4◻μl of each) and purified using AMPure XP beads (Beckman Coulter, California, United States) according to manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2020Quote: ... cells were centrifuged at 4 ℃ for 5 minutes at 300g and suspended in HBSS and analyzed with a flow cytometer (CytoFLEX, Beckman Coulter). Mean fluorescence intensity was calculated for each group.
-
bioRxiv - Systems Biology 2020Quote: ... the 500xg supernatant was first spun at 10,000xg for 10 minutes at 4°C (Beckman Coulter, fixed angle rotor TLA 120.2) in 1 ml 11×34 mm polycarbonate uncapped centrifuge tubes (Beckman Coulter 343778) ...
-
bioRxiv - Systems Biology 2020Quote: ... each resultant fraction underwent separate ultracentrifugation at 100,000xg for 40 minutes at 4°C (rotor TLA 120.2) in 1 ml 11×34 mm polycarbonate uncapped centrifuge tubes (Beckman Coulter 343778). In EV-enriched pooled fraction experiments ...
-
bioRxiv - Systems Biology 2020Quote: ... and underwent ultracentrifugation at 100,000xg for 40 minutes at 4°C (rotor MLA-55; Beckman Coulter 355603 10.4 ml capped tubes). Supernatant was discarded from each fraction ...
-
bioRxiv - Cell Biology 2020Quote: ... 50 µl of alkaline carbonate treated mitochondria were set aside as input (kept on ice) and 50 µl were centrifuged at 100 000g for 30 min at 4°C in TLA100 rotor (Beckman Coulter). Pellet was gently washed with ice-cold sodium carbonate buffer and resuspended in 20 µl of 1x Laemmli loading buffer ...
-
bioRxiv - Microbiology 2020Quote: ... cells were fixed with 4% FA in PBS for 90 min at room temperature and immunostained for intracellular HIV-1 CA for 30 min at 4°C using KC57-FITC antibody (Beckman Coulter) diluted in 0.1% Triton X-100 ...
-
bioRxiv - Immunology 2020Quote: ... during 30min and then transferred into a DuraClone IF T Helper cell tube (CD3, CD4, IL-4, IL-17A, IFNγ) (Beckman Coulter), using the IntraPrep fixation permeabilization kit (Beckman Coulter) ...
-
bioRxiv - Bioengineering 2022Quote: ... Cells were subsequently incubated with 2.5×103 CFSE-RJ-EVs per cell for 4 hours and analyzed with flow cytometry (CyAn ADP, Beckman Coulter, US). Treatment groups were compared to unstained control as well as CFSE-RJ-EVs without inhibitor ...
-
bioRxiv - Cell Biology 2022Quote: ... The supernatant was recovered and centrifuged at 60,000 rpm for 15 min at 4°C in an Optima TL micro-ultracentrifuges (TLA 100.3 rotor Beckman Coulter, Fullerton, CA) to obtain the Golgi-enriched p100 pellet fraction ...
-
bioRxiv - Physiology 2022Quote: ... 40 ml of filtrates were ultracentrifuged twice in sterile 10.4 mL polycarbonate bottles at 100 000 x g for 70 min at 4°C using an Optima L-100 XP Ultracentrifuge equipped with 90 Ti Fixed-Angle Titanium Rotor (Beckman Coulter). The final EVs pellets were suspended in 200 µl of PBS (Lonza) ...
-
bioRxiv - Microbiology 2022Quote: ... respectively) by centrifugation at 4000 × rpm for 10 min at 4 °C using an Allegra® X-15R centrifuge (Beckman Coulter) and flash-frozen in liquid nitrogen before RNA isolation ...
-
bioRxiv - Biophysics 2024Quote: ... The virions were harvested from the supernatant by centrifugation through 1.5ml of 30% sucrose at 10,000 RPM at 4°C for 2hrs (Beckman L8-70M Ultracentrifuge). The resulting pellet of virions was resuspended in 20ul of RIPA lysis buffer containing protease inhibitors.
-
bioRxiv - Molecular Biology 2024Quote: ... for ultracentrifugation at 100,000 rcf for 70 min at 4 °C using a Optima XE-90 ultracentrifuge with a SW 32 Ti rotor (Beckman Coulter). The EV pellet was resuspended in 0.22 μm-filtered sterile PBS and freshly-used or stored at −80 °C until further use ...
-
bioRxiv - Cell Biology 2024Quote: ... The samples were ultracentrifuged at 39,000 rpm at 4°C for 2.5 h in an SW41 rotor (Beckman Coulter, California, USA). Finally ...
-
bioRxiv - Microbiology 2024Quote: ... Most of the supernatant was discarded leaving 12x∼200 µL of concentrated broken pieces of genomic fiber that were pooled and layered on top of ultracentrifuge tubes of 4 mL (polypropylene centrifuge tubes, Beckman Coulter) containing a discontinuous cesium chloride gradient (1.4 ...
-
bioRxiv - Cancer Biology 2024Quote: ... CRC cells were then cocultured at a 1:1 ratio with BMDCs for 4 hours and analyzed for uptake of CRC DNA by flow cytometry on the CytoFlex S cytometer (Beckman Coulter). Analysis was performed in FlowJo.
-
bioRxiv - Cell Biology 2024Quote: ... Samples were then spun at 100,000 xg for 16 h at 4°C using a TLS-55 swinging-bucket rotor and benchtop ultracentrifuge (Optima Max-XP, Beckman Coulter). Fractions were collected and loaded in SDS-PAGE for WB.
-
bioRxiv - Biochemistry 2023Quote: ... The sample was run on the gradient for 16 hrs at 4 °C using an Optima XE-90 ultracentrifuge (SW40Ti rotor, Beckman-Coulter) at 30,000 rpm ...
-
bioRxiv - Genomics 2023Quote: ... cellular/protein debris were then collected by centrifugation at 1,000xg for 15 minutes at 4°C (Beckman Coulter Allegra X-14R), and the supernatant (DNA ...
-
bioRxiv - Biochemistry 2023Quote: ... then centrifuged at 27,000 gmax for 15 min at 4°C (Optima XPN-100 Ultracentrifuge, Beckman Coulter, with SW60 Ti rotor). Resuspension of the pellet (sub-mitochondrial particles ...
-
bioRxiv - Cancer Biology 2024Quote: ... cells were resuspended in 100µL of PBS and stored at 4°C in the dark until samples were analyzed on a CytoFLEX LX Flow Cytometer (Beckman Coulter). Data was analyzed using FlowJo® software.
-
bioRxiv - Bioengineering 2024Quote: ... we first measured a cell line population in PBS suspension using a coulter counter (Beckman Coulter Multisizer 4, 100 μm aperture) to obtain a cell volume distribution with the standard unit ...
-
bioRxiv - Cell Biology 2024Quote: ... We loaded 2 mL of the homogenate mixture on top of a 3.92 ml 62% sucrose in MAB cushion in an Ultra-Clear 5/8” X 4” Beckman Coulter tube (344061, Beckman Coulter). We added the remaining sucrose in MAB solutions ...
-
bioRxiv - Biochemistry 2024Quote: ... Non-solubilized materials were removed by centrifuging the sample at 41,000 r.p.m for 1 h at 4 °C using rotor type 45 Ti in the OptimaTM L-100 XP Ultracentrifuge (BECKMAN COULTER). The resulting supernatant containing the solubilized LysP-GFP was incubated with Ni2+-NTA resin (Qiagen ...
-
bioRxiv - Microbiology 2024Quote: ... Samples were balanced and centrifuged at 42,000 rpm for 35m at 4°C in a SW55Ti rotor and buckets with tube adaptors (Beckman REF: 356860). The supernatant was then concentrated to 80μl by spinning through Amicon 3kDa MWCO concentrator units at 14,000xg for 15-30m at 4°C ...
-
bioRxiv - Microbiology 2024Quote: ... and labeled intracellularly for the structural HIV-1 p24 protein with the anti-p24 clone KC57 antibody (30 min RT followed by 30 min 4°C, Beckman Coulter). HIV-1 RNA probing was performed using the PrimeFlow RNA Assay (ThermoFisher) ...
-
bioRxiv - Microbiology 2024Quote: Bacterial isolates were grown overnight on LB agar and DNA extraction was performed using the GenFind v3 gDNA extraction kit according to standard protocol (Supp. Table 4) (Beckman Coulter). For the mock microbial community ...
-
bioRxiv - Biochemistry 2023Quote: ... Colorimetric changes was measured 3 min after mixing the conditioned media with DTNB solution at 450 nm of absorbance (DTX880, Beckman Coulter, Indianapolis, IN, USA). The value from DTNB only was substracted as noise ...
-
bioRxiv - Microbiology 2019Quote: ... Gradients were then spun at 38,000 RPM for 2 h in a SW41Ti rotor (Beckman Coulter; 331362) at 4°C ...