Labshake search
Citations for Beckman :
1651 - 1700 of 1935 citations for 6H Pyrazolo 3 4 g benzothiazole 2 methyl 8CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Ribosomes were isolated by sucrose cushion ultracentrifugation at 100,000 rpm at 4°C for 1 h using TLA110 rotor (Beckman Coulter). RNAs in the ribosome pellet were isolated with TRIzol-LS (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... Cell debris and unbroken cells were removed by centrifugation at 2,000 xg for 10 minutes and membranes were collected by ultracentrifugation at 186,000 xg for 1 hour at 4°C with a 45 Ti rotor (Beckman Coulter). The collected membrane was homogenized using a Dounce homogenizer in cold TBS buffer containing 1 mM PMSF ...
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bioRxiv - Neuroscience 2023Quote: ... The gradients were centrifuged for 18 h at 100,000 xg at 4 °C in a SW 40 rotor (Beckman Coulter). 500 µl fractions were collected from the top of the ultracentrifuge tubes and analyzed by WB analysis.
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bioRxiv - Cell Biology 2022Quote: ... Lysates were cleared by centrifugation for 30 min at 4°C at 18,000 rpm (SS-34 rotor; Beckman-Coulter, USA).
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bioRxiv - Cancer Biology 2023Quote: ... Samples homogenized in lysis buffer were loaded onto a 5%-20% linear gradient and centrifugated at 37,000 rpm for 170 min at 4°C in an SW41Ti rotor (Beckman Coulter).
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bioRxiv - Cell Biology 2023Quote: ... down to 6 ml and ultracentrifuged at 10,000xg for 16 min at 4°C in MLA-80 rotor (Beckman Coulter). The pellet was resuspended and washed with PBS at the same conditions (volume ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... The whole cell homogenate was layered on top of 2 ml shelf of 0.8 M sucrose in HB and centrifuged at 2000 rpm for 10 min at 4°C in a JS5.2 rotor (Beckman Coulter) to yield a low speed nuclear pellet and a postnuclear supernatant ...
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bioRxiv - Bioengineering 2023Quote: ... The final supernatant was centrifuged at 100,000g at 4°C for 70 min using an ultracentrifuge (Optima MAX-XP Tabletop Ultracentrifuge, Beckman Coulter). The pellet was either used immediately or stored dry at −80°C.
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bioRxiv - Biochemistry 2023Quote: ... The reaction mixture was applied onto the top of the gradient solution and was centrifuged at 41,000 rpm at 4 °C for 17 hrs using an SW41 Ti rotor (Beckman Coulter). The gradient was fractionated using a Piston Gradient Fractionator (BioComp) ...
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bioRxiv - Cell Biology 2023Quote: ... and then centrifuged for 150 min at 39,000 rpm and 4°C in an Optima L-100XP Ultracentrifuge (Beckman-Coulter) using a SW41Ti rotor ...
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bioRxiv - Biophysics 2023Quote: ... at 4°C until equilibrium was reached using an An Ti 60 rotor in an XL-I analytical ultracentrifuge (Beckman), run using ProteomeLab XL-A/XL-I (version 6.2) ...
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bioRxiv - Molecular Biology 2023Quote: ... for 45[min at 25°C and then subjected to a sucrose cushion ultracentrifugation at 100,000[rpm for 1[h at 4°C with Optima MAX-TL ultracentrifuge and TLA-110 rotor (Beckman Coulter). The RNA fragments in the resulting pellet were recovered using TRIzol reagent (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and samples were centrifuged at 24,000 rpm at 4°C for 1h in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter). After discarding the supernatant ...
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bioRxiv - Immunology 2023Quote: ... virus solutions were clarified of debris by centrifugation at 10,000 rpm at 4°C for 30 minutes in a chilled SW32Ti rotor (Beckman Coulter), followed by pelleting through a 30% cold sucrose solution at 25,000 rpm for 90 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 200µl BD 1X Perm/Wash solution and stained 1h at 4°C with anti-HIV-1 p24 (RD1/PE, Beckman Coulter #6604667 ...
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bioRxiv - Biochemistry 2023Quote: ... The diluted extract was then centrifuged at 31,200 rpm for 30 minutes at 4°C in a Type 70 Ti rotor (Beckman Coulter). The supernatant was filtered through a 0.45 µm syringe filter (Millex-GP PES membrane (Millipore SLHP033RS ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was centrifuged at 40,000 rpm for 60 min at 4°C (XPN-100 ultracentrifuge, Type 45 Ti rotor, Beckman, USA). The supernatant was filtered through a 0.45 μm filter (Pall ...
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bioRxiv - Bioengineering 2023Quote: ... The final supernatant was centrifuged at 100,000g at 4°C for 70 min using an ultracentrifuge (Optima MAX-XP Tabletop Ultracentrifuge, Beckman Coulter). The pellet was either used immediately or stored dry at −80°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Filtered supernatants were concentrated by ultracentrifugation at 22.000 rpm for 2h at 4°C with OptimaTM L-90K ultracentrifuge (Beckman Coulter) and stored at −80°C (never refrozen).
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bioRxiv - Cell Biology 2023Quote: ... and pelleted at 100,000xg for 20 min at 4 °C using fixed angle rotor (TLA-100 rotor, Beckman Coulter, 349481) in a benchtop ultracentrifuge (OptimaTL ...
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bioRxiv - Cell Biology 2023Quote: ... Equilibrated tubes were ultra-centrifuged at 100,000xg for 1h at 4°C using a swinging-bucket rotor (SW 32Ti, Beckman Coulter, 369650; Optima XE, Beckman Coulter). Phagosome-containing beads float at the interface between the 10% and 25% layers ...
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bioRxiv - Biophysics 2023Quote: ... The lysate was clarified by centrifugation at 40,000 rpm for 35 min at 4°C (Type 45 Ti Rotor, Beckman Coulter). The resulting supernatant was mixed with 1ml pre-washed IgG Sepharose 6 FastFlow beads (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... 20 mL of supernatant was gently layered on top of 10mL of 20% sucrose dissolved in PBS (4°C, sterile filtered) in an ultra-clear 1×3.5′ centrifuge tube (Beckman 344058). The tubes were spun down in a SW28 rotor (Beckman ...
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bioRxiv - Neuroscience 2024Quote: ... and samples were centrifuged at 24,000 rpm at 4°C for 1h in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter). After discarding the supernatant ...
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bioRxiv - Cell Biology 2022Quote: ... The product from PCR-2 was purified using 1x volume of AMPure XP beads (Beckman Coulter) and eluted in 15 μL of IDTE buffer (IDT DNA) ...
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bioRxiv - Bioengineering 2021Quote: ... The supernatant was then ultracentrifuged for 2 hours using Optima™ L-80XP Ultracentrifuge (Beckman Coulter) at 100,000 x g at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The solution was centrifuged at 36,000 rpm for 2 h in a SW41Ti rotor (Beckman Coulter) at 4°C ...
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bioRxiv - Genetics 2021Quote: ... We purified the final libraries with 2 rounds of 0.8X volume of SPRISelect (Beckman Coulter, B23318).
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bioRxiv - Immunology 2020Quote: ... were incubated with 1-2 million 1D8 cells following standard FACS protocol and analyzed by Beckman.
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... Gradients were centrifuged at 35,000 rpm for 2 hrs 40 min using a SW41Ti rotor (Beckman). Gradients were fractionated using a Brandel (UV ...
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bioRxiv - Microbiology 2023Quote: ... Three 1:2 serial dilutions were done suing a Biomek i7 liquid handler (Beckman Coulter, USA), in order to obtain the four working concentrations ...
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bioRxiv - Cell Biology 2022Quote: ... Equal volume (2 ml i.e 1600 µg) of cell extracts were loaded in ultracentrifuge tubes (Beckman). Samples were ultracentrifuged at 100,000 g for 30 minutes at 4°C in a Optima XPN-100 ultracentrifuge (Beckman) ...
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bioRxiv - Bioengineering 2023Quote: ... in 2%BSA and 2mM EDTA and sorted using FACS sorter (Beckman Coulter, MoFlo Astrios Zellsortierer). For gating ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 2% BSB-PFA and analysed by Flow Cytometry on BD Celesta instrument (Beckman Coulter) using FlowJoTM software ...
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bioRxiv - Immunology 2023Quote: Cells were washed with 2% FACs and data was acquired using CytoFLEX S (Beckman Coulter, USA) and analyzed via FlowJo v10 software (Oregon ...
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bioRxiv - Biochemistry 2021Quote: The absorbance spectra of recombinant DosS and DosT (3 µM) were determined at room temperature using quartz cuvettes with rubber stoppers in a DU800 spectrophotometer (Beckman Coulter, Fullerton, CA). The reduction of recombinant DosS was achieved via addition of sodium dithionite (DTH ...
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bioRxiv - Microbiology 2022Quote: ... induced by doxycycline (DOX) at 3 μg/ml and sorted for high GFP-expressing cells by MoFLO Astrios EQs (Beckman Coulter Life Sciences) using 488 nm lasers ...
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bioRxiv - Bioengineering 2023Quote: ... the supernatant was then taken and loaded into a 10% to 45% sucrose gradient solution and centrifuged at 360000 rpm for 3 h (Beckman Optima XE-100). 18 fractions from each sample were collected using a piston gradient fractionator (Biocomp ...
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bioRxiv - Microbiology 2024Quote: ... reinhardtii cell density and size were determined using a hemocytometer and ImageJ or a Beckman Coulter Multisizer 3 with a 50 μm orifice (Beckman Coulter, CA, USA). Bacterial cell density was determined by counting colony forming units (CFU ...
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bioRxiv - Plant Biology 2021Quote: ... the samples were centrifuged at 35 000 RPM for 18 hours at 4°C in a 70Ti rotor and L8-M ultracentrifuge (Beckman Coulter). The ribosome pellets were then gently washed with RNase-free water and resuspended in 300 μl of resuspension buffer (100 mM Tris·HCl (pH 8.0) ...
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bioRxiv - Molecular Biology 2021Quote: ... The gradient was ultracentrifuged at 55,000 rpm for 55 min at 4 °C with max acceleration and slow deceleration using an Optima LE-80K Ultracentrifuge and SW55Ti rotor (Beckman Coulter). The sucrose gradient was fractionated according to the number of associated ribosomes as determined by the profile of the absorbance at 254 nm using a Density Gradient Fractionation System (Brandel ...
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bioRxiv - Molecular Biology 2021Quote: ... pombe cells were pelleted down for 10 min at 5000 rpm at 4°C (Rotor: F10-6X500, FiberLite Beckman J2-MC). The pellet was washed with buffer 1 (HEPES- 20 mM ...
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bioRxiv - Bioengineering 2022Quote: ... The 60-70 mL were then divided equally into two 50-mL centrifuge tubes and spun down outside the anaerobic chamber at 4 °C and 3500 rpm for 10 min using an Allegra 64R ultracentrifuge (Beckman Coulter). Next ...
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bioRxiv - Bioengineering 2022Quote: ... Each 250 mL culture was divided equally into six 50-mL centrifuge tubes and spun down at 4 °C and 3500 rpm for 10 min using an Allegra 64R centrifuge (Beckman Coulter). The supernatant was discarded and 6 mL of ice-cold 10% glycerol was used to wash and combine the six cell pellets into one suspension ...
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bioRxiv - Genomics 2020Quote: ... Lentiviral supernatant was collected at 48 and 72hrs post-transfection and subsequently concentrated by ultracentrifugation (24,000 rpm, 4°C, 2hrs) (Beckman Coulter SW32).
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bioRxiv - Biophysics 2019Quote: AUC-SV analyses were performed in XL-A analytical ultracentrifuge equipped with a 4-hole An-60 Ti rotor and 12-mm double-sector charcoal-filled epon centerpieces (Beckman Coulter). Sedimentation velocity measurements using absorbance optics of reference buffer and samples in 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Molecular Biology 2019Quote: ... The cleared supernatant was layered over 15-40% sucrose gradients in disassociation buffer and spun 28000 rpm for 16 hours at 4°C in a SW-32 rotor (Beckman-Coulter). Gradients were fractionated with an ISCO fractionation system and 30S + 50S peaks were combined ...
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bioRxiv - Genetics 2020Quote: ... The homogenates were incubated on ice for 15–20 min and then centrifuged at 3000xg for 10 min at 4°C in a GPR Data Settop centrifuge (Beckman, Inc.). The supernatant solutions were centrifuged at 15,800xg for 15 min at 4°C in a microcentrifuge (Model 5042 ...
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bioRxiv - Biochemistry 2020Quote: ... in BC300N buffer and centrifuged for 4hr at 55,000rpm at 4°C in a TLS-55 rotor (with Beckman 356860 adaptors). 40µl fractions were collected by pipetting from the top ...
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bioRxiv - Biophysics 2021Quote: Purified 3C DNA from each sample was sonicated to 200-400 bp with a Soniprep 150 probe sonicator at 4°C and purified with a standard Ampure XP Bead protocol (Beckman Coulter) using a 1/1.5 DNA to bead ratio ...