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Citations for Beckman :
1251 - 1299 of 1299 citations for Borrelia burgdorferi C p Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... Following incubation (37 °C, 20-24 h), the samples were ultracentrifuged (1h, 288’000 x g) (Optima TLX Ultracentrifuge, Beckman Coulter, TLA-120.2 Beckman rotor) to remove large aggregates ...
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bioRxiv - Physiology 2019Quote: ... Samples were then centrifuged at 100,000 xG at 4 °C for 20 hours in a swinging bucket rotor (Beckman L8-M Ultracentrifuge, SW28 Rotor).
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bioRxiv - Biochemistry 2019Quote: ... and 5 mM MgCl2 at pD 7.5 corresponding to a pH reading of 7.1) at 25°C as follows: spin column at 2000 rpm for 2 min in a tabletop centrifuge (Beckman Coulter Allegra R Tabletop Centrifuge), discard the flow-through ...
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bioRxiv - Cell Biology 2020Quote: ... Bacteria were harvested by centrifugation at 4000 rpm for 25 min at 4°C in a Beckman J6M clinical centrifuge (Beckman Coulter Diagnostics; Brea, California), and re-suspended on ice in lysis buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... and layered on top of a two-step sucrose gradient (20%/50% w/v in HNE) and centrifuged for 2 h at 154 000 x g at 4 °C in a SW41 rotor (Beckman Coulter, Woerden, the Netherlands) to clear from cell debris ...
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bioRxiv - Systems Biology 2020Quote: ... and the resultant supernatant was ultracentrifuged at 100,000xg for 40 minutes at 4°C (rotor TLA 120.2; Beckman Coulter 343778 1 ml uncapped tubes).
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bioRxiv - Physiology 2021Quote: ... sEV were further isolated from lEV-depleted supernatants following a 100,000 xg ultracentrifugation step for 1 hour at 4°C (rotor MLA-50, Beckman Coulter Optima MAX-XP Ultracentrifuge) and two washes in NaCl before resuspension in NaCl.
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bioRxiv - Neuroscience 2022Quote: ... The samples were then spun at 31000 x g (18468 rpm) for 5 min at 4°C in a fixed-angle rotor (50.2 Ti rotor in Beckman Coulter Optima L-100 XP) with slow brake ...
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bioRxiv - Biophysics 2023Quote: ... Cell debris was removed by centrifugation at 20,000 rpm at 4°C for 1 h (Avanti® J-E centrifuge from Beckman Coulter, rotor: JLA 25.50). The supernatant was collected and loaded onto a pre-equilibrated 5 ml His-Trap FF crude column ...
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bioRxiv - Biochemistry 2023Quote: ... cells were treated with the drugs (20 µM to a suspension at 500,000 cells/mL in PBS containing 2 % FBS at 37°C) and directly analyzed by flow cytometry (Gallios, Beckman-Coulter, 405/488 nm laser) over 2 h ...
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bioRxiv - Microbiology 2023Quote: ... and 1.45 g/mL layers and centrifuged for 1 h at 28,500 rpm at 15 °C using an Optima XE-90 Ultracentrifuge and SW 41 Ti Swinging-Bucket Rotor (Beckman Coulter, Inc., Pasadena, CA, USA). After centrifugation ...
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bioRxiv - Molecular Biology 2023Quote: ... and were centrifuged at 120,000 g for 120 minutes at 4°C with a MLA55 fixed-angle rotor using an Optima MAX-XP ultracentrifuge (Beckman Coulter, California, United States). The pelleted hVSMCs EVs were resuspended in 100 μL PBS and washed in 13.2 mL PBS by ultracentrifugation at 120,000 g for 60 minutes at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... placed inside 250 mL centrifuge bottles and centrifuged for 10 min at 100 g at 4 °C (JA-14 rotor, Avanti J-20 XP Centrifuge; Beckman Coulter, Indianapolis, IN, USA). LSW was then filtered through a 0.2 µm syringe filter (Acrodisc syringe filter ...
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bioRxiv - Genomics 2024Quote: ... cDNA release and collection is performed by incubating the chip in cDNA release mix overnight at 55*C and the release cDNA is purified with Ampure XP beads (Beckman Coulter; Cat. No. A63882) using the manufacturer’s recommendation ...
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bioRxiv - Microbiology 2024Quote: ... EMVs were obtained by ultracentrifugation from 3 mL of filtrate at 100,000 × g (average centrifugal force) and 4 °C for 2 h with 50Ti rotor and Optimax centrifuge (Beckman Coulter, Brea, CA, United States). The supernatant after removing EMVs was collected as PVF ...
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bioRxiv - Neuroscience 2022Quote: ... The amount of p24 capsid protein was determined by the HIV-1 p24 ELISA antigen assay (Beckman Coulter, Fullerton, CA). Virus from different productions averaged 175 ng/µl of p24 antigen.
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bioRxiv - Biochemistry 2021Quote: ... 300 μL of metabolite pool was aliquoted per target protein replicate and sealed with aluminum foil seals (Beckman Coulter 538619) to create the metabolite chambers ...
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bioRxiv - Cell Biology 2023Quote: ... and the uptake of endocytosed fluorescent proteins was analyzed in CD42b-positive population using a CytoFlex S flow Cytometer (Beckman).
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bioRxiv - Microbiology 2023Quote: ... Not solubilised protein was removed by a second pelleting at 100×kg for 1 h in Ti70 rotor (Beckman Coulter). The solubilized and denatured protein was loaded on a 5 ml HisTrap HP column (Cytiva) ...
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bioRxiv - Molecular Biology 2023Quote: ... the extract and the protein ladder were overlaid onto separate gradients and centrifuged with an SW41Ti rotor (Beckman Coulter, 20U113807) in an Optima XPN-100 Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Cell Biology 2024Quote: Cell populations were analyzed for fluorescent protein expression using a CytoFLEX S flow cytometer (Beckman Coulter, San Jose, CA, USA). Single cell suspensions were isolated from each experimental condition 3-5 days after transfection ...
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bioRxiv - Immunology 2024Quote: ... and stained for Gag p24 protein with anti-HIV-1 core antigen monoclonal antibody (clone KC57, Beckman Coulter, cat. 6604665).
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bioRxiv - Cancer Biology 2020Quote: ... Filtered media containing lentiviral particles were centrifuged at 24000rpm at 20°C for 2 hrs in 90Ti rotor in ultra-centrifuge (Beckman Coulter Optima TM L-100K). Viral particle pellet was obtained after discarding supernatant post centrifugation and suspended in 100 μl OptiMEM media for overnight at 4°C.The collected lentivirus suspension was used for transfection or stored for later use at −80°C ...
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bioRxiv - Biochemistry 2022Quote: ... Around 100 µL of sample at 15 µM was applied to each gradient and centrifuged by ultracentrifugation at 45 000 rpm at 4°C in SW 60 Ti Swinging-bucket rotor (Beckman Coulter, Palo Alto, US-CA) for 16 hours ...
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bioRxiv - Microbiology 2020Quote: ... Prepared phage suspension was transferred to U-formed centrifuge tubes suitable for ultra-centrifugation (25 000 × g, 120 min, 4 °C) (Beckman Coulter, Optima™ MAX-XP). Pellets were resuspended in 200 µL of SM buffer and phage stock suspensions were stored at 4°C and −80°C.
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bioRxiv - Neuroscience 2019Quote: ... The ultracentrifuge tubes were centrifuged at 124000 x g at 4 ° C for 30 minutes in Optima L-100XP ultracentrifuge (Beckman Coulter, SW 55 Ti rotor). The resulting pellet was suspended in 50 μL of TKM with protease inhibitors and commercial phosphatases (78440 ...
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bioRxiv - Biochemistry 2019Quote: ... SS-34 rotor) at 10,000 rpm at 4°C for 1 hours followed by 1 hour of ultracentrifugation (Beckman Optima™ LE, 80K, SW28 rotor) at 28,000 rpm (104000 x g ...
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bioRxiv - Cell Biology 2021Quote: ... This lysate was sonicated for 2 min (15 s on and 20 s off for 2 min at 20% amplitude) and centrifuged at 100,000 x g for 40 min at 4°C (Avanti J-301 Beckman Coulter centrifuge with rotor JA-30.50). The supernatant was filtered and passed through preequilibrated 5 ml HisTrap FF columns (GE Healthcare) ...
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bioRxiv - Biophysics 2022Quote: ... Labeled G-actin was clarified to remove any remaining F-actin or precipitated FMAL by centrifugation (10 min, 90,000 rpm in a Beckman TLA-120.2 rotor at 4°C). The extent of labeling (typically around 70% ...
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bioRxiv - Molecular Biology 2024Quote: ... placed at the bottom of the tube and centrifuged at 5,000x g for 15 min at 16 °C in a swinging bucket rotor (Beckman Coulter, JS-5.3 or JS-7.5). The coverslips were recovered and fixed with pre-chilled methanol (–20 °C ...
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bioRxiv - Biochemistry 2024Quote: ... Pellets were washed and gently resuspended in 1 mL of PBS and centrifuged at 50,000 x g for 1 h at 4°C (Beckman Optima MAX-E, TLA-55 rotor). Pellets were resuspended in 500 μl of PBS to obtain concentrated lentiviral stocks that were stored at −80°C in aliquots ...
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bioRxiv - Biochemistry 2024Quote: ... NaCl was then added to 1 M and the lysed solution was centrifuged at 20,000 g at 1°C for 45 minutes (Beckman Coulter J2-21, JA 20 rotor). Tween-20 was added to the supernatant to 0.1% (v/v ...
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bioRxiv - Biophysics 2023Quote: ... Cells were harvested by centrifugation at 8000 g at 4°C for 20 min (Avanti® J-E centrifuge from Beckman Coulter, rotor: JLA 16.250).
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bioRxiv - Biochemistry 2023Quote: ... Pellets were washed and gently resuspended in 1 ml of PBS and centrifuged at 50,000 × g for 1 h at 4°C (Beckman Optima MAX-E, TLA-55 rotor). The resulting pellets were resuspended in 500 μl of PBS to obtain concentrated lentiviral stocks that were stored at −80°C in aliquots ...
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bioRxiv - Bioengineering 2023Quote: ... subtilis spores were collected from the culture by the centrifugation at 8000 rpm at 4 °C for 20 min (Avanti J-E, Beckman Coulter Life Science, IN, USA). Supernatant was removed and the spore pellets were resuspended in fresh 100 mM PBS pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... lysosomes and mitochondria were enriched by centrifugation of the pooled PNS at 56,500 ×g for 7 min at 4 °C (Beckman-Coulter, 70 Ti fixed-angle rotor). The supernatant was removed ...
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bioRxiv - Biochemistry 2022Quote: ... 750 µL of the 80% Histodenz solution were added and thoroughly mixed with 750µL of the protein-LUV sample in Thinwall centrifuge tubes (Beckman 50.2ti rotor). 1.5 mL of the 30% Histodenz solution was added as the intermediate layer ...
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bioRxiv - Biochemistry 2020Quote: ... Protein samples were clarified by centrifugation using a TLA-55 rotor in an Optima MAX-XP table top ultracentrifuge (Beckman Coulter) (90,720 x g ...
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bioRxiv - Immunology 2022Quote: ... and the expression of the Gag- p24 viral protein was detected with a PE-anti-p24 antibody (clone KC57 RD1; Beckman Coulter). Cell viability was determined using an aqua viability dye for flow cytometry (LIVE/DEAD® Fixable Aqua Dead Cell Stain kit ...
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bioRxiv - Neuroscience 2024Quote: ... Biological titers of the viral preparations were assessed by transducing HEK293T cells with serial dilutions and checking the percentage of green fluorescent protein (GFP)-positive cells by flow cytometry (Cytomics FC 500, Beckman Coulter). Hippocampal-cultured neurons were transduced at 7DIV with lentiviral vectors (1-2 transducing units/cell).
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bioRxiv - Biochemistry 2023Quote: The tendency of the protein to form aggregates was determined by dynamic light scattering (DLS) in the Delsa Max Core (Beckman Coulter). Prior to measurement ...
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bioRxiv - Pathology 2023Quote: ... the proportion of HIV-1-infected cells was determined by intracellular staining of the viral protein Gag-p24 with a Mouse anti-p24-FITC antibody (clone KC57, Beckman Coulter).
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bioRxiv - Biochemistry 2019Quote: ... centrifuged at 2000 × g for 10 min at room temperature and the plasma was separated and stored at −20°C until analysis on clinical chemistry analyzer (Beckman Coulter AU5800, Beckman Coulter Inc. Brea, CA; HUCFF-UFRJ), using kinetic colorimetric assays for α-amylase (Beckman OSR6006 ...
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bioRxiv - Neuroscience 2020Quote: ... for further depletion of cell debris and spun at 10,000 × g for 30 min at 4°C (AH-650 rotor, Beckman Ultra-Clear Tube with 5 ml capacity). The pellet was resuspended in 100 μl PBS and considered to be the “10K pellet” (10K or 10K fraction) ...
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bioRxiv - Immunology 2023Quote: ... UC-EVs were obtained by centrifuging 50 mL of conditioned media at 100,000 × g for 70 min at 4 °C (AH-629/36 rotor, Beckman Ultra-Clear Tubes with 36 ml capacity). Pellet was re-suspended in 250 µL of DPBS ...
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bioRxiv - Microbiology 2023Quote: ... briefly mixed and equilibrated for ∼30 min (Albumax equilibration was conducted in a 5% CO2 incubator to maintain pH 7.4) after which they were subjected to ultracentrifugation at 37°C using a sealed rotor (Beckman Rotor type 42.2 Ti; 223,000 x g) for 4.2 h to separate proteins ...
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bioRxiv - Biochemistry 2020Quote: ... Protein expression was induced with 0.4 mM Isopropyl β-D-1-thiogalactopyranoside and cells were grown at 37 °C overnight before harvesting by centrifugation at 4000 rpm (Beckman Coulter Avanti JXN-26; JLA8.1 rotor) at 10 °C for 10 min.
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bioRxiv - Biochemistry 2019Quote: ... Protein expression was induced with 0.4 mM Isopropyl β-D-1-thiogalactopyranoside and cells were grown at 25 °C overnight before harvesting by centrifugation at 4000 rpm (Beckman Coulter Avanti JXN-26; JLA8.1 rotor) at 4 °C for 15 min.
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bioRxiv - Genomics 2019Quote: ... Membranes and other cellular debris were sedimented away from soluble protein on a Beckman J2-21 large floor centrifuge (Beckman Coulter, Brea, CA). Supernatant containing the recombinant protein was incubated with 10 mL of precleared Ni-NTA beads for 10min at 4°C ...