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Citations for Beckman :
951 - 1000 of 1229 citations for Borrelia burgdorferi C p Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... Gradients were ultra-centrifuged at 221,600 × g for 2 hours at 4°C (SW-41Ti rotor, 331362, Beckman Coulter, Brea, CA). Gradients were fractionated (Piston Fractionator ...
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bioRxiv - Genomics 2019Quote: ... Cleared cell extracts were ultracentifuged at 34400 rpm for 2 hours 40 minutes at 4C using a C-1000 XP centrifuge with SW40 rotor (Beckman Coulter). Gradient UV absorption at 254 nm was measured and selected fractions were selected for 5’cap library preparation (5µg purigied RNA per sample) ...
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bioRxiv - Molecular Biology 2020Quote: ... the lysate was incubated under rotation for 30 min at 4 °C and cleared for 10 min in an MLA-80 rotor (Beckman Coulter) at 11,500 xg and 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was ultracentrifuged at 120,000 g for 3 h at 4°C using an MLS-50 rotor (Beckman Coulter, USA) to collect 120k pellet vesicles ...
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bioRxiv - Immunology 2021Quote: ... To sort specific subsets of C-LP mononuclear phagocytes cells were gated as above and sorted by MoFlo Astrios Sorter (Beckman Coulter).
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bioRxiv - Biochemistry 2019Quote: ... Gradients were centrifuged at 49,123 x g for 16 hours at 4°C using an SW32Ti rotor (Beckman Coulter, ref. 369694). Gradients were fractionated with a Brandel gradient fractionation system using a 1 mm UV cell detector ...
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bioRxiv - Systems Biology 2020Quote: ... the 500xg supernatant was first spun at 10,000xg for 10 minutes at 4°C (Beckman Coulter, fixed angle rotor TLA 120.2) in 1 ml 11×34 mm polycarbonate uncapped centrifuge tubes (Beckman Coulter 343778) ...
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bioRxiv - Microbiology 2019Quote: ... saliva EVs were pelleted by ultracentrifugation at 100,000 × g for 90 min at 4 °C (Optima™ Max-XP, Beckman Coulter). The EVs were washed in 2 ml PBS and pelleted again at 100,000 × g for 90 min and suspended in PBS ...
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bioRxiv - Systems Biology 2020Quote: ... each resultant fraction underwent separate ultracentrifugation at 100,000xg for 40 minutes at 4°C (rotor TLA 120.2) in 1 ml 11×34 mm polycarbonate uncapped centrifuge tubes (Beckman Coulter 343778). In EV-enriched pooled fraction experiments ...
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bioRxiv - Systems Biology 2020Quote: ... and underwent ultracentrifugation at 100,000xg for 40 minutes at 4°C (rotor MLA-55; Beckman Coulter 355603 10.4 ml capped tubes). Supernatant was discarded from each fraction ...
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bioRxiv - Biophysics 2019Quote: ... Samples were loaded onto the gradient and spun at 15°C for 4h at 140,000g using a SW28 rotor (Beckman Coulter, CA). The VLPs appeared as an opaque band at the interface of the 1.25 and 1.4 g/ml CsCl layers and were collected by piercing the tube with an 18G needle and syringe ...
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bioRxiv - Genetics 2020Quote: ... Reverse crosslinking reactions were incubated 2 h at 65°C and cleaned using Agencourt Ampure XP SPRI beads (Cat #A63880, Beckman Coulter) with a 2x bead:sample ratio ...
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bioRxiv - Cell Biology 2020Quote: ... 50 µl of alkaline carbonate treated mitochondria were set aside as input (kept on ice) and 50 µl were centrifuged at 100 000g for 30 min at 4°C in TLA100 rotor (Beckman Coulter). Pellet was gently washed with ice-cold sodium carbonate buffer and resuspended in 20 µl of 1x Laemmli loading buffer ...
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bioRxiv - Microbiology 2020Quote: ... Vesicles were obtained by centrifugation at 38,400 x g for 1 h at 4°C in an Avanti J-20XP centrifuge (Beckman Coulter, Inc). Pelleted vesicles were resuspended in 20 ml of 50 mM HEPES (pH 6.8 ...
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bioRxiv - Microbiology 2021Quote: ... with a final extension at 72 °C for 10 min PCR products were purified using Agencourt AMPure XP beads (Beckman Coulter) ratio 0.6× ...
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bioRxiv - Microbiology 2020Quote: ... cells were fixed with 4% FA in PBS for 90 min at room temperature and immunostained for intracellular HIV-1 CA for 30 min at 4°C using KC57-FITC antibody (Beckman Coulter) diluted in 0.1% Triton X-100 ...
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bioRxiv - Immunology 2020Quote: Intracellular cytokine production by T cells was analyzed after stimulating PBMC or whole blood (fresh or frozen) 4h at 37°C in DurActive1 tubes (Beckman Coulter), which contain PMA ...
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bioRxiv - Molecular Biology 2021Quote: ... The samples were then separated into membrane and matrix fractions by ultracentrifugation at 100 000 g (TLA 110 rotor) for 30 min at 4°C in OptimaTM TLX table-top ultracentrifuge (Beckman-Coulter). The supernatant fraction was transferred to new tube ...
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bioRxiv - Immunology 2020Quote: ... and the boundary layers between the two sucrose layers were harvested after ultracentrifugation (90 min, 51,000 rpm, 18 °C, Optima™ L90K centrifuge, rotor type: 70.1 Ti, tubes: 16 * 76 mm, Beckman Coulter, USA). These fractions were subsequently mixed with 6 M CsCl (AppliChem ...
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bioRxiv - Immunology 2021Quote: ... Samples were spun at 76,650 x g for 2 hours at 4° C inside a SW 32i rotor using an Optima L-90K Ultracentrifuge (Beckman Coulter). Purified virus was quantified using a bicinchoninic acid assay (BCA ...
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bioRxiv - Immunology 2021Quote: ... Insoluble proteins and cell debris were removed by centrifugation (13,000 rpm, 30 minutes at 5°C, JA-30.50Ti rotor, Beckman, Palo Alto, CA). The soluble CuMVTT CP protein in clarified lysate was pelleted using saturated ammonium sulfate (1:1 ...
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bioRxiv - Genomics 2021Quote: ... The samples were then centrifuged at 28,600 rpm for 2 h at 4°C (Beckman Optima XE-90; SW32 Ti rotor). After centrifugation ...
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bioRxiv - Immunology 2020Quote: ... Vesicles recovered by ultracentrifugation of filtered supernatant at 150,000 x g for 2 h at 4 °C using a Ti 45 rotor (Beckman Instruments, USA), were washed with phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2022Quote: Whole bovine milk samples (200 mL) were centrifuged at 3,000 x g for 15 min at 4°C (Allegra X-15R, Beckman Coulter, France) to separate the fat from the skimmed milk ...
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bioRxiv - Cell Biology 2022Quote: ... After ultracentrifugation at 200,000 x g for 5 h at 4°C (SW55Ti rotor in a XL-90 Beckman Coulter centrifuge), fractions containing purified EVs or purified phages were extracted from the top following the scheme in Supp ...
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bioRxiv - Biochemistry 2022Quote: ... Soluble and insoluble fractions were isolated via centrifugation (90,000 x g, 30 min, 4 °C) in an Optima™ MAX-OP (Beckman Coulter) centrifuge using the TLA-100.3 rotor ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was recovered and centrifuged at 60,000 rpm for 15 min at 4°C in an Optima TL micro-ultracentrifuges (TLA 100.3 rotor Beckman Coulter, Fullerton, CA) to obtain the Golgi-enriched p100 pellet fraction ...
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bioRxiv - Physiology 2022Quote: ... 40 ml of filtrates were ultracentrifuged twice in sterile 10.4 mL polycarbonate bottles at 100 000 x g for 70 min at 4°C using an Optima L-100 XP Ultracentrifuge equipped with 90 Ti Fixed-Angle Titanium Rotor (Beckman Coulter). The final EVs pellets were suspended in 200 µl of PBS (Lonza) ...
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bioRxiv - Genomics 2022Quote: ... The eluted chromatin was reverse-crosslinked overnight at 65°C and the DNA was purified with AMPure XP beads (Beckman Coulter).
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bioRxiv - Microbiology 2022Quote: ... respectively) by centrifugation at 4000 × rpm for 10 min at 4 °C using an Allegra® X-15R centrifuge (Beckman Coulter) and flash-frozen in liquid nitrogen before RNA isolation ...
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bioRxiv - Neuroscience 2022Quote: ... The discontinuous sucrose gradient was centrifuged at 242K g for 47 min at 4 °C in a vertical rotor (VTi50 rotor, Beckman Coulter). The bulk GC fraction (GCF ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were then submitted to centrifugation at 100,000 g for 45 min at 4°C in an Ultracentrifuge Beckman XL-90 (Beckman France, Villepinte) equipped with a 70.1T1 rotor ...
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bioRxiv - Microbiology 2023Quote: ... viruses were collected and concentrated by ultracentrifugation (37,000 rpm, 2 h 30, 15 °C; Beckman Type 45 Ti fixed-angle rotor). The virus titer was determined using a plaque assay.
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bioRxiv - Physiology 2023Quote: ... was ultracentrifuged twice in sterile 10.4 mL polycarbonate bottles at 100 000 × g for 70 min at 4°C (Optima L-100 XP Ultracentrifuge, 90 Ti Fixed-Angle Titanium Rotor; Beckman Coulter). The final EV pellet was suspended in 50 µl of PBS without Ca2+ and Mg2+ (Lonza ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatant were loaded on top of a discontinuous 0.75/1.0/2.33 M sucrose density gradient and centrifuge at 242,000 x g for 60 min at 4°C (Beckman SWTi41 swing rotor). The growth cone depleted fraction was collected between 0.75 M and 1.0 M sucrose ...
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bioRxiv - Microbiology 2023Quote: ... The isopycnic centrifugation of DNA was performed with an initial CsCl buoyant density of 1.725 g mL-1 subjected to centrifugation at 177,000 × g for 36-40 h at 20 °C in an Optima XPN-80 ultracentrifuge (Beckman Coulter, USA). At the end of the centrifugation ...
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bioRxiv - Cell Biology 2023Quote: ... Homogenates were then transferred to a 50 ml tube and centrifuged for 10 mins at 1 000 × g at 4°C (Beckman Coulter). The resulting supernatant was then transferred to microfuge tubes for storage at -80°C as whole liver lysates or to fresh ultracentrifuge tubes for further processing.
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bioRxiv - Cell Biology 2023Quote: ... The cilia in the upper phase were sedimented by centrifugation (27,000 g, 4°C, 20 min; Beckman Coulter, Avanti JXN-26) and resuspended in Hepes-Magnesium-EGTA-Potassium (HMEK ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was run on the gradient for 16 hrs at 4 °C using an Optima XE-90 ultracentrifuge (SW40Ti rotor, Beckman-Coulter) at 30,000 rpm ...
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bioRxiv - Biophysics 2023Quote: ... the SWCNT suspension was centrifuged at 30000 rpm (164 000 x g) for 4 h at 25 °C (Optima XPN-80, Beckman Coulter) to remove any remaining nanotube aggregates.
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bioRxiv - Cancer Biology 2023Quote: ... Crosslinks were reversed with 25 µg Proteinase K for 16 hours at 65°C and DNA was purified using AmpureXP beads (Beckman Coulter). Enrichment was confirmed using qRT-PCR with known positive and negative binding sites for each protein target ...
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bioRxiv - Biophysics 2022Quote: ... EVs were separated from protein aggregates and free reagents by bottom-up density gradient centrifugation in sucrose for 17.30 h at 192,000 g and 4°C using a SW40 rotor (k-factor 144.5; Beckman Coulter, Fullerton, California, USA). Twelve fractions of 1 mL were then collected from the top of the gradient and respective densities were determined by refractometry using an Atago Illuminator (Japan) ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting supernatant was filtered with a membrane pore size of 0.2 μm (Whatman) and centrifuged at 120,000 × g for 4 h at 4 °C in polycarbonate bottle assemblies (Beckman coulter, 355603) in a 70.1 Ti fixed angle rotor (Beckman coulter ...
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bioRxiv - Biophysics 2023Quote: ... and concentrated by several washing steps in FBS-free Opti-MEM® and virus particles were concentrated by ultracentrifugation on a 60% (w/V) iodixanol cushion at 21°C (131,500 x g, 90 min, SW28 rotor, Beckman Coulter, Ireland) to a final volume of 1 mL ...
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Expanding the toolbox for phycobiliprotein assembly: phycoerythrobilin biosynthesis in SynechocystisbioRxiv - Synthetic Biology 2023Quote: ... Ultracentrifugation was performed in a SW40 Ti swinging-bucket rotor at 130000 × g for 16 h at 20 °C (Optima L-80 XP, Beckman Coulter).
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bioRxiv - Plant Biology 2023Quote: ... The cAW was collected by spinning at 700 x g for 20 min at 4 ⁰C (JA-14 rotor, Beckman Coulter; Avanti J-20TM centrifuge, Beckman Coulter). vAW was filtered through a single layer of Miracloth and was combined with the cAW ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were pelleted by centrifugation at 12,000 xg and 4 °C for 10 min in an Avanti J26-XPI centrifuge (Beckman Coulter Inc.). The cell pellets were homogenized in lysis buffer (25 mM Tris ...
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bioRxiv - Microbiology 2023Quote: ... The normalized cultures were then centrifuged at 5030 x g and 4°C for 20 minutes on an Avanti J-26 XPI (Beckman-Coulter) centrifuge (JS 5.3 rotor) ...
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bioRxiv - Bioengineering 2023Quote: ... The gradient tubes were balanced and subjected to centrifugation at 135,000 × g for 2 h at 4 °C using a fixed-angle rotor (TLA-55, Beckman Coulter, USA). After DGUC ...
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bioRxiv - Bioengineering 2023Quote: ... The gradient tubes were subjected to centrifugation at 135,000 × g for 2 h at 4 °C using a fixed-angle rotor (TLA-55, Beckman Coulter, USA). After DGUC ...