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Citations for Beckman :
901 - 950 of 1229 citations for Borrelia burgdorferi C p Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... This was incubated for 1 hour at room temperature while rotating before centrifugation at 100,000 x g for 90 minutes at 4°C using the Optima™ MAX-XP Ultracentrifuge (Beckman Coulter). The supernatant was then collected (sarkosyl-soluble fraction) ...
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bioRxiv - Molecular Biology 2021Quote: ... pombe cells were pelleted down for 10 min at 5000 rpm at 4°C (Rotor: F10-6X500, FiberLite Beckman J2-MC). The pellet was washed with buffer 1 (HEPES- 20 mM ...
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bioRxiv - Bioengineering 2022Quote: ... The 60-70 mL were then divided equally into two 50-mL centrifuge tubes and spun down outside the anaerobic chamber at 4 °C and 3500 rpm for 10 min using an Allegra 64R ultracentrifuge (Beckman Coulter). Next ...
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bioRxiv - Bioengineering 2022Quote: ... Each 250 mL culture was divided equally into six 50-mL centrifuge tubes and spun down at 4 °C and 3500 rpm for 10 min using an Allegra 64R centrifuge (Beckman Coulter). The supernatant was discarded and 6 mL of ice-cold 10% glycerol was used to wash and combine the six cell pellets into one suspension ...
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bioRxiv - Genomics 2020Quote: ... Lentiviral supernatant was collected at 48 and 72hrs post-transfection and subsequently concentrated by ultracentrifugation (24,000 rpm, 4°C, 2hrs) (Beckman Coulter SW32).
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bioRxiv - Cancer Biology 2019Quote: ... betulina (LB) after oven dried at 50°C temperature for 48 h were ground by mixture grinder (Beckman food mixer, India). This smashed biomass (100 g ...
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bioRxiv - Molecular Biology 2019Quote: ... The cleared supernatant was layered over 15-40% sucrose gradients in disassociation buffer and spun 28000 rpm for 16 hours at 4°C in a SW-32 rotor (Beckman-Coulter). Gradients were fractionated with an ISCO fractionation system and 30S + 50S peaks were combined ...
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bioRxiv - Immunology 2021Quote: ... were incubated with monoclonal antibodies at 4°C for 30 min and then fixed with 4% paraformaldehyde before analysis on a Cytoflex cytometer (Beckman Coulter). Data were analyzed by Flow Jo software v.10.7 (BD Biosciences) ...
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bioRxiv - Genetics 2020Quote: ... The homogenates were incubated on ice for 15–20 min and then centrifuged at 3000xg for 10 min at 4°C in a GPR Data Settop centrifuge (Beckman, Inc.). The supernatant solutions were centrifuged at 15,800xg for 15 min at 4°C in a microcentrifuge (Model 5042 ...
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bioRxiv - Plant Biology 2020Quote: ... The loaded density gradients were centrifuged at 33,000 g for 14.5 h at 4°C using a six-position SW41 Ti rotor (Beckman Coulter, Kerfeld, Germany). The positions of ribosome complexes varied slightly with each centrifugation run that comprised a set of six samples ...
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bioRxiv - Bioengineering 2020Quote: ... Ultracentrifugation was performed at 100,000 x g at 4 C for 45 min in an Optima MAX-XP ultracentrifuge (Beckman Coulter, Inc.). Supernatant was discarded ...
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bioRxiv - Biochemistry 2020Quote: ... in BC300N buffer and centrifuged for 4hr at 55,000rpm at 4°C in a TLS-55 rotor (with Beckman 356860 adaptors). 40µl fractions were collected by pipetting from the top ...
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bioRxiv - Biophysics 2021Quote: Purified 3C DNA from each sample was sonicated to 200-400 bp with a Soniprep 150 probe sonicator at 4°C and purified with a standard Ampure XP Bead protocol (Beckman Coulter) using a 1/1.5 DNA to bead ratio ...
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bioRxiv - Biophysics 2021Quote: ... transferred to 15 mL conical tube and centrifuged at 233 x g for 5 min at 22 °C using a swinging bucket rotor (Beckman Coulter). Pelleted cells were resuspended and washed once with 1x PBS (-CaCl2 ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was then collected and subjected to ultracentrifugation at 100,000 x g for 70 min at 4°C using an Optima XPN-80 ultracentrifuge with SW 32 Ti swinging rotor (Beckman Coulter). Pellets were washed in PBS 1X and EVs were subjected to an additional centrifugation at 100,000 x g for 30 min at 4°C using an Optima MAX ultracentrifuge ...
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bioRxiv - Neuroscience 2020Quote: ... centrifuged at 100,000 g for 70 min at 4°C using an Optima XPN-80 ultracentrifuge with SW 32 Ti swinging rotor (Beckman Coulter) and then processed for further analyses.
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bioRxiv - Plant Biology 2021Quote: ... Syringes were placed inside 50-mL tubes and centrifuged for 20 min at 700g with slow acceleration (4°C, JA-14 rotor, Avanti J-20 XP Centrifuge; Beckman Coulter). The apoplastic wash fluid was then filtered through a 0.22 μm membrane and centrifuged at 10,000g for 30 minutes to remove any remaining large particles ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM DTT) and centrifuged for 2 hours and 15 minutes at 39000 rpm at 4°C (Beckman Coulter TLS55 rotor). Fractions corresponding to the large mitochondrial subunit were collected and subjected to buffer exchange (25 mM HEPES-KOH pH = 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... The first PCR reaction was performed for 15 cycles with 60 °C annealing and 30 s elongation and then purified with AMPure XP PCR beads (Beckman Coulter). The second PCR was performed for 15 cycles for wild type-specific and for 21 cycles for tag-specific amplicons respectively using 60°C annealing and 30 s elongation ...
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bioRxiv - Molecular Biology 2021Quote: ... and spung for 20hrs at 151,000 x g (rav) at 4°C in a swinging bucket rotor (Beckman SW-41 Ti). 12 1ml fractions were taken off the gradient and incubated for 10min at RT with 100μl 0.15% deoxycholine ...
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bioRxiv - Cell Biology 2021Quote: ... or Lin−Sca-1+c-Kit+CD150+CD48−CD34− (CD34−HSC) and Lin−Sca-1+c-Kit+ (LSK) cells were sorted on MoFlo Astrios EQ (Beckman Coulter). Quantification of CD71 ...
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bioRxiv - Microbiology 2021Quote: ... and cytoplasmic membranes were precipitated by ultracentrifugation for 45 min at 55,000 rpm and 4°C (Beckman Coulter rotor TLA 55). Membrane fractions resuspended in resuspension buffer (750 mM aminocaproic acid ...
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bioRxiv - Biophysics 2021Quote: ... The crude lysate was sonicated on ice and clarified by centrifugation for 30 min at 4°C and 35,000 rpm in a Beckman L90K Optima and 50.2 Ti rotor (Beckman, Brea, CA, USA). The clarified lysate was applied to a HisTrapHP column (Cytiva ...
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bioRxiv - Biophysics 2021Quote: ... and the fractions containing the protein of interest were combined and concentrated to 2mL at a final concentration of 3.85 mg/mL by repeated centrifugation in an Amicon Ultra Centrifugal Filter at 4k rpm for 5 min at 4 °C (Beckman SX4250). The concentrated protein was incubated overnight with TEV (1:50 mass ratio ...
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bioRxiv - Cell Biology 2021Quote: ... and loaded onto of a step-wise sucrose gradient as described by the manufacturer and subjected to ultracentrifugation at 150,000 xg for 4 h at 4 °C using SW50.1 rotor (Beckman Coulter, Mississauga, ON). Fractions were then collected and subject to denaturation with Laemmli buffer until further use.
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bioRxiv - Biophysics 2020Quote: ... and clearance was determined by monitoring Optical Density (DO) at 325 nm and 24°C in a Beckman Coulter DTX 880 Microplate Reader (Beckman, CA). Curves were adjusted by fitting to a single exponential decay y = y0 + ae−bx.
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bioRxiv - Biophysics 2022Quote: ... and the supernatant was subjected to ultracentrifugation to separate the membrane fraction (100,000g, 1 hour, 4°C using an Optima XE-90, Beckman Coulter centrifuge). Membranes were resuspended in lysis buffer supplemented with cOmplete EDTA-free protease inhibitors (Roche) ...
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bioRxiv - Microbiology 2022Quote: ... through a syringe needle (0.4-by 20 mm needles) and the cell wall was collected by ultracentrifugation at 90,000 × g for 45 min at 4°C (Beckman Coulter, Inc.). To the pellet ...
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bioRxiv - Bioengineering 2022Quote: ... The final supernatant was collected and EVs were pelleted at 100,000 g at 4°C for four hours in an Optima ultracentrifuge using the Beckman Ti45 rotor (Beckman Coulter, USA). The EVs pellet was washed with PBS and resuspended in 0.22 μm filtered PBS ...
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bioRxiv - Cell Biology 2022Quote: ... A new supernatant was collected in a prechilled 1.5 ml ultracentrifuge tube and centrifuged at 100000 g for 1 h at 4°C in a TLA-55 rotor (Beckman Coulter). The resulting pellet was resuspended in the same isotonic buffer containing antiproteases and was frozen at -80°C together with the 100000 g supernatant ...
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bioRxiv - Cancer Biology 2022Quote: ... Putative LSC-enriched populations were isolated from freshly expanded MLL-AF9 mouse leukemia cells by sorting c-Kithigh (top 10%) cells on a MoFlo XDP cell sorter (Beckman Coulter). Sorted cells were plated in triplicate in semi-solid methylcellulose medium (Methocult M3434 ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were incubated for 2h at 20°C in an AN60-Ti rotor in the Optima-AUC analytical ultracentrifuge (Beckman Coulter) before data acquisition for 15h at 42,000 rpm ...
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bioRxiv - Microbiology 2021Quote: ... Cell supernatants containing the pseudotype viral particles were centrifuged at 109,000 g or 35 min at 4 °C using a TLA100.3 rotor with an Optima TLX ultracentrifuge (Beckman Coulter, CA, USA), and the pellet was then lysed for western blotting analysis.
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... and centrifuged at 4°C at 31,000 rpm for 2 h and 30 min using an SW 32 Ti rotor(Beckman Coulter, 369694). The gradients were fractionated using a Teledyne ISCO Density Gradient Fractionation System and 12 fractions were collected ...
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bioRxiv - Molecular Biology 2020Quote: ... The filtered supernatant was then centrifuged in JA20 tubes at 6000 rpm overnight (O/N) at 4°C (Avanti J-25, Beckman Coulter). The following day the viral pellet was resuspended in 5 ml PBS and centrifuged at 20,000 rpm for 90 mins at 4°C (Optima XL-100K ...
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bioRxiv - Molecular Biology 2019Quote: ... Centrifugation was performed at 22 °C for 3 h at 217 874 g using a SW 40 Ti rotor (Beckman Coulter). Fractions of 600 µl were carefully collected manually ...
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bioRxiv - Molecular Biology 2020Quote: ... The gradients were centrifuged at 40000 rpm for 2h at 4°C in a SW 60 Ti swinging-bucket rotor (Beckman Coulter). Afterwards ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 100,000 g at 4°C for 60 min in a swinging rotor (SW40Ti) (Beckman Coulter OptimaTM L–100K). Following centrifugation ...
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Single cell analysis of the cellular heterogeneity and interactions in the injured mouse spinal cordbioRxiv - Neuroscience 2020Quote: ... were added to each sample well and cells were kept at 4°C until analysis on a Cytoflex Flow Cytometer (Beckman Coulter).
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bioRxiv - Cell Biology 2020Quote: ... were pelleted in Beckman Coulter polypropylene open top tubes (110,000 x g for 70 min at 4°C, in Beckman rotor SW28). After washing ...
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bioRxiv - Microbiology 2022Quote: ... and the viral particles were concentrated by ultracentrifugation for 1h at 30,000 rpm at 4°C in an SW40 rotor (Beckman, Fullerton, CA). Viral pellets were resuspended in TNC buffer (10 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Genetics 2022Quote: ... A final DNase treatment was performed in 50 μL total volume using 1 μL of Turbo DNase incubated for 4 hours at 37°C followed by inactivation with 1 μL of 10% SDS and purification using RNA Ampure XP beads (Beckman Coulter).
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bioRxiv - Developmental Biology 2022Quote: ... proteinase K and heat for de-crosslinking (overnight at 65°C) followed by ethanol precipitation and SPRI bead clean up (Beckman Coulter). The resulting DNA was quantified by Clariostar (BMG Labtech) ...
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bioRxiv - Microbiology 2022Quote: ... Samples were incubated for 2 h at 16°C and double stranded cDNA was purified using AMPure XP beads (Beckman Coulter). For testing transcription activity of predicted genes ...
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bioRxiv - Cancer Biology 2022Quote: ... Crude vesicle pellets were resuspended in 1 ml of exosome-depleted 1X PBS and re-pelleted by centrifugation at 100,000 g for 70 minutes at 4°C (Beckman 45 Ti). Pellets at the bottom of the centrifugation tubes were resuspended in approximately 50ul of 1X PBS ...
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bioRxiv - Systems Biology 2020Quote: ... The resultant supernatant was collected and underwent ultracentrifugation at 100,000xg for 40 minutes at 4°C (rotor MLA-55) in 10.4 ml 16×76 mm polycarbonate capped centrifuge tubes (Beckman Coulter 355603), the pellet was washed once in PBS−/− and centrifuged again at 100,000xg for 40 minutes at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... EDS was obtained after 18 h ultracentrifugation at 100,000 g and 4 °C (SW55 Ti rotor, Beckman Coulter, Fullerton, California, USA), followed by 0.22 µm filtration ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by 3 h ultracentrifugation at 100,000 g and 4 °C using SW 32.1 Ti rotor (Beckman Coulter, Fullerton, California, USA). Resulting pellets were resuspended in 100 µl PBS and stored at −80 °C until further use ...
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bioRxiv - Microbiology 2019Quote: ... The concentrated vesicles were washed twice with PBS by ultracentrifugation at 110,000xg at 4°C for 1 hour (Beckman Optma MAX-XP Ultracentrifuge, rotor TLA 120.2; Beckman Coulter Inc., USA). The final pellet (exosomes ...
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bioRxiv - Biophysics 2019Quote: ... to final volume of 12 ml and then spun at 15°C for 16hr at 155,000g using a SW 41Ti rotor (Beckman Coulter, CA). The VLPs were detected at the lower part of the tube and recovered as described above ...