Labshake search
Citations for Beckman :
901 - 944 of 944 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The remaining PCR reaction of samples treated with a single gRNA was subjected to purification with AMPure XP reagent (Beckman Coulter) according to the manufacturer’s manual using AMPure beads ...
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bioRxiv - Molecular Biology 2023Quote: ... Size selection purifications following the ligation and amplification PCR steps were performed with 0.9× reaction volumes of Agencourt AMPure XP beads (Beckman Coulter A63880). Purified libraries were combined as an 8-sample equimolar pool containing a combination of indexes from 1–12 and sequenced on an Illumina NextSeq on an Illumina NextSeq 2000 on a P2 flow cell (paired-end 75-bp reads).
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bioRxiv - Genomics 2023Quote: ... The PCR amplification is followed by a bead clean up step with 1X sample-to-bead ratio AMPure XP beads (Beckman Coulter), binding at room temperature for 5 min ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The PCR amplicons of the samples were then pooled after a purification and concentration equalization process with the AMPureXP Kit (Beckman Coulter). The libraries were processed in an Illumina MiSeq v2 (500 cycles and paired-end).
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bioRxiv - Developmental Biology 2024Quote: ... Post-capture PCR enrichment was followed by final purification of the libraries with AMPure XP Beads (Beckman Coulter, catalog no. A63881). Library quality was evaluated using Bioanalyzer profiles (Agilent Technologies) ...
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bioRxiv - Genomics 2024Quote: ... Sequencing libraries were generated using a PCR-free approach (33) and then cleaned up using Agencourt AMPure XP SPRI beads (Beckman Coulter). The resulting libraries were sequenced on the Illumina NovaSeq platform at the Wellcome Sanger Institute ...
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bioRxiv - Plant Biology 2024Quote: ... The index PCR was stopped after 8–10 cycles and the library DNA was purified using AMPure XP (A63880, Beckman Coulter). The library was sequenced on an Illumina NovaSeq 6000 instrument (150-bp paired-ends ...
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bioRxiv - Immunology 2024Quote: ... The libraries were pooled in the VBLOK200 reservoir (ClickBio) by spinning the PCR II plate upside down and purified using 1X AMPure XP beads (Beckman Coulter). Final libraries were sequenced on Illumina NovaSeq platform at 300 000 reads per well.
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bioRxiv - Immunology 2024Quote: ... The N26 barcode was PCR amplified using the above extracted plasmid as the template and final PCR products were purified by Ampure XP beads (Beckman Coulter), quantified ...
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bioRxiv - Neuroscience 2024Quote: ... the reaction was added 1 µL of 0.2µM HTO primer (5’-GTGACTGGAGTTCAGACGTGTGC*T*C) prior to PCR cycling and cleaned up using SPRISelect magnetic beads (Beckman Coulter, #B23318), 80% ethanol ...
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bioRxiv - Neuroscience 2020Quote: ... we generated double-stranded DNA template for transcription of each sgRNA via a template-free polymerase chain reaction (PCR) with partially overlapping primers (IDT, PAGE purified) and purified the product with Ampure XP beads (Beckman Coulter, A63880). We then transcribed sgRNAs in vitro using the MEGAscript T7 Transcription Kit (ThermoFisher Scientific ...
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bioRxiv - Genomics 2021Quote: Sequencing library amplified from the second round of PCR were size-selected and purified with a magnetic bead-based SPRIselect reagent (Beckman Coulter, B23318), and subjected to HiSeq4000 Illumina NGS platform using a single read (SR75 ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were purified with the kit from Agencourt AMPuer XPsystem (Cat. No. A63880, Beckman Coulter, South San Francisco, CA, USA), and the purified products were used with the second PCR amplification ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we applied 1.8× (after end repair) and 1.0× (after adapter ligation and PCR) volumes of Agencourt AMPure XP (Beckman Coulter, Cat. No. A63880). The optimal number of PCR cycles for library amplification was determined by a preliminary quantitative PCR using KAPA HiFi HotStart Real-Time Library Amplification Kit (KAPA ...
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bioRxiv - Immunology 2020Quote: ... The resultant products were column-purified and PCR-enriched to generate final Illumina-compatible libraries (Agencourt AMPure XP, Beckman Coulter, Brea, CA). The libraries from young peri-implant tissues (n=6 ...
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bioRxiv - Genomics 2021Quote: ... with 5 PCR reactions per column then pooled together and cleaned up a second time with 1.6X SPRI beads (Beckman Coulter, Brea, California). The CHEQ-seq vector containing a random 17 bp BC upstream of the synthetic intron was linearised via inverse PCR with primers “CHEQseq_lin_A_For” and “CHEQseq_lin_A_Rev” or “CHEQseq_lin_B_For” and “CHEQseq_lin_B_Rev” ...
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bioRxiv - Synthetic Biology 2023Quote: ... The PCR products were analyzed with 2% agarose gel and transferred into 96-well PCR plate wells for clean-up with 20 µL of AMPure XP beads (Beckman Coulter #A63881) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Triplicate PCR reactions for each sample were then pooled and purified using Agencourt® AMPure® XP beads (#A63881, Beckman Coulter, UK).
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bioRxiv - Microbiology 2023Quote: ... The 230bp band was verified to be present and the amplified PCR products were cleaned up using double-sided SPRI via AMPure Beads (Beckman Coulter, A63880). Purified samples were normalized to a concentration of 10 nM using Qubit dsDNA HS Assay Kit (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... PCR reactions were pooled into eight 1.5 mL Eppendorf tubes (600 uL) with an equal volume of SPRI (Beckman-Coulter, Brea, CA) beads that incubated for 5 minutes at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was examined on gel electrophoresis and cleaned up by the AMpure XP beads (PCR product: bead 1:1 v:v, Beckman Coulter, Krefeld, Germany). The generated libraries were pooled with equal molar DNA ...
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bioRxiv - Microbiology 2022Quote: ... Then 40 µL of the elution was transferred to a fresh PCR tube and subjected to a 0.6x Cleanup with AMPure XP beads (Beckman Coulter, CN A63881). The cDNA was then eluted in 30 µL nuclease-free water.
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bioRxiv - Microbiology 2022Quote: ... The V4 region of 16S rRNA gene was amplified using the universal primer set 515F and 806R.27 PCR products were cleaned up with an AMPure XP kit (Beckman Coulter, CA), followed by electrophoresis on 0.7% agarose gel and 1.15% Synergel (Diversified Biotech ...
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bioRxiv - Microbiology 2023Quote: ... The PCR product (barcoded 16S amplicon) was cleaned up from the PCR reaction mixtures using AMPure XP Solid Phase Reversible Immobilization (SPRI) paramagnetic beads (Beckman-Coulter #A63880). 50 µL of the PCR reaction mixture containing the amplified product was mixed with equal volume of SPRI bead suspension and a magnetic separation rack was used to separate DNA-bound beads from rest of the solution ...
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bioRxiv - Genomics 2023Quote: ... The tagmented DNA was amplified with eight cycles of PCR amplification and the libraries were cleaned up with SPRselect beads (Beckman Coulter, #B23317). The mean size of the library molecules and the quality of the libraries were determined on an Agilent Bio-analyser High Sensitivity DNA chip (Agilent technologies ...
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bioRxiv - Genetics 2024Quote: We separate the resulting product into long (“L”) and short (“S”) molecular libraries from the same PCR reactions by using SPRIselect beads (Beckman Coulter, #B23319). The next step involves further preparing libraries for long-read sequencing ...
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bioRxiv - Systems Biology 2024Quote: ... The supernatant was then transferred to a 96-well PCR plate and centrifuged at 2100 g (Allegra X-12R, Beckman Coulter Inc.) for 3 min ...
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bioRxiv - Neuroscience 2020Quote: ... Sequencing products were cleaned up using magnetic beads (CDTR-0050; CleanNA, Waddinxveen, The Netherlands) and according to the Agencourt AMpure PCR purification (Beckman Coulter, Brea, USA) protocol ...
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bioRxiv - Microbiology 2021Quote: ... Equimolar concentrations of PCR products were purified and pooled using a 0.8 volume of AMPure XP beads (Beckman Coulter Genomics, Danvers, MA, USA) and eluted with molecular water ...
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bioRxiv - Synthetic Biology 2022Quote: ... 20 μL reactions were prepared by dispensing 1 μL of each 10 μM reverse primer into the wells of a 96-well PCR plate using the Echo liquid handler (Beckman Coulter, Brea, CA). A mastermix consisting of polymerase premix ...
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bioRxiv - Microbiology 2020Quote: ... The DNA from the 50 μL PCR reactions was purified using 60 μL Agencourt AMPure XP beads following manufacturers protocols (Beckman Coulter no. A63880). For the second PCR ...
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bioRxiv - Immunology 2021Quote: ... as well as the mRNA PCR product were separated and purified by double-sided size selection using AMPure XP magnetic beads (Cat. No. A63880; Beckman Coulter, Krefeld, Germany). A fraction of the Abseq/multiplex sample tag PCR 1 product ...
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bioRxiv - Genomics 2024Quote: ... Illumina P5/P7 adapters were added during sample index PCR and the final libraries were cleaned using SPRIselect beads (Beckman Coulter cat. #B23318). For snRNA-seq ...
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bioRxiv - Cancer Biology 2024Quote: 4ul each of the resultant libraries (from each well of the PCR plate) are pooled into a 1.5 ml tube and cleaned using AMPure XP beads (Beckman Coulter Life Sciences, IA). The quality of the pooled libraries is assessed on tapestation ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Junction PCR products were purified with a 0.8x bead to sample volume ratio with AMPure XP Reagent beads (Beckman Coulter cat. no. A63881). Either 10 ng plasmid DNA (input ...
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bioRxiv - Evolutionary Biology 2021Quote: ... one half was purified using silica columns (MinElute PCR Purification Kit) and the other using 3x SPRI beads (Beckman Coulter™ Agencourt AMPure XP). Indexing PCR ...
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bioRxiv - Molecular Biology 2021Quote: The VHH-coding regions within parent libraries and 1-round target-enriched sublibraries were PCR amplified and purified using AMPure XP beads (Beckman Coulter, High Wycombe, UK). Then ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Barcoded PCR products (234 bp) were pooled from triplicate tubes and purified by double-sided size selection using Ampure beads (Beckman Coulter, Pasadena, CA, USA). In brief ...
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bioRxiv - Microbiology 2022Quote: ... The success of the amplifications was checked in a 2% agarose gel and PCR products were subsequently purified using Agencourt AMPure XP magnetic beads kit (Beckman Coulter, Brea, CA, USA), quantified with the QuantiFluor™ dsDNA System (Promega ...
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bioRxiv - Microbiology 2022Quote: ... and (III) the PCR products as the final construction of the libraries were purified with AMPure XP system (Beckman Coulter Inc., Indianapolis, IN, USA). Sequencing library size distribution quality control was performed with an Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Cancer Biology 2023Quote: The VHH-coding regions within the parent (mother) libraries and the target-panned sublibraries were PCR amplified and purified using AMPure XP beads (Beckman Coulter, High Wycombe, UK). Dual-indexed libraries were prepared and sequenced on an Illumina MiSeq37 (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... Any amplicon contaminated with traces of non-specific fragments was further purified using the Agencourt AMPure™ PCR purification kit (Beckman Coulter, Beverly, MA, USA). The concentration of each PCR amplicon was determined using the Quant-iT™ PicoGreen kit (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... The final RNA-Seq library was obtained after 15 cycles of PCR amplification and purified using Agencourt AMPure XP beads (Beckman and Coulter, Beverly, MA, USA). The library size distribution was evaluated by running samples on the Agilent 2100 Bioanalyzer ...
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bioRxiv - Microbiology 2021Quote: ... Each PCR amplicon was cleaned twice to remove the primers and short DNA fragments using the Agencourt AMPure XP system (Beckman Coulter, Inc., Brea, CA, USA) and quantified using a Qubit Fluorometer (Invitrogen Corporation ...