Labshake search
Citations for Beckman :
751 - 800 of 944 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... PCR products from all wells were pooled and purified with 1× Agencourt AMPure XP beads (Beckman Coulter) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... The resulting PCR amplicons were cleaned-up with AMPure XP bead-based reagents (Beckman Coulter Life Science), DNA concentration and fragment distribution quantified on a fragment analyzer (Agilent ...
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bioRxiv - Cancer Biology 2024Quote: ... The PCR product was purified with 1.8 times the volume of Ampure XP beads (Beckman Coulter #A63881). Library quality was assessed using a BioAnalyzer 2100 High Sensitivity DNA Chip (Agilent Technologies) ...
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bioRxiv - Neuroscience 2024Quote: The indexed PCR products were pooled in a single tube and 0.8X Ampure XP purification (Beckman Coulter) was carried out as per manufacturer’s recommendation and finally the sequencing library was eluted in 35.5 ul of Elution buffer (Qiagen).
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bioRxiv - Synthetic Biology 2024Quote: ... PCR products were then pooled and purified using 2.0x AMPure XP beads (Beckman Coulter, Cat. No. A63880), then BsaI digested and ligated (NEB ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR products were then pooled and purified using 2.0x AMPure XP beads (Beckman Coulter, Cat. No. A63880), then BsaI digested and ligated (NEB ...
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bioRxiv - Genetics 2024Quote: ... PCR products were purified with Agencourt AMPure XP SPRI beads according to manufacturer’s instructions (Beckman Coulter, A63880). Samples were sequenced using Illumina technology (either MiSeq ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR products were then pooled and purified using 2.0x AMPure XP beads (Beckman Coulter, Cat. No. A63880), then BsaI digested and ligated (NEB ...
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bioRxiv - Neuroscience 2020Quote: ... Final 15 mL of solution was mixed by inverting the tube 10x and carefully pipetted into 2 ultracentrifuge tubes (Beckman Coulter #344059) onto the isolation buffer cushion (5 mL ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Clarified supernatants were layered over 2 ml of a 25% (w/v) sucrose solution in 13.2 ml Thinwall Polypropylene SW41 Ti ultracentrifuge tubes (Beckman-Coulter, Brea, USA) and made up to a total volume of 10 ml with PBS without calcium chloride or magnesium chloride (PBS[-]) ...
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bioRxiv - Genetics 2022Quote: ... The samples were size selected with 0.65x volume of Agencourt® AMPure® XP magnetic bead solution (Beckman Coulter, Brea, CA, USA), and suspended in 20µL elution buffer (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... The nuclei suspension was layered onto 10 mL of 29% OptiPrep solution (Buffer + 50% OptiPrep) in a 38.5 mL Ultra-Clear tube (Beckman-Coulter, CA, USA) before being centrifuged at 7,333g for 30 minutes at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... The combined cAW and vAW solutions were then centrifuged using 29 x 104 mm (50 mL) polypropylene centrifuge tubes (Beckman Coulter, # 357007) at 10,000 x g for 1 h at 4 ⁰C (JA-17 rotor ...
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bioRxiv - Plant Biology 2023Quote: ... and 2 mL of 5% solution in a 14 x 89 mm (13.2 mL) thin walled ultra-clear centrifuge tubes (Beckman Coulter, Cat. # 344059). Approximately 250 µl of crude EV sample resuspended in 20 mM Tris-HCl pH 6.0 was slowly dispensed on top of the column and centrifuged at 100,000 x g for 17 h at 4 ⁰C (SW 41 Ti rotor ...
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bioRxiv - Cell Biology 2023Quote: ... saturated solution) to reach a final sucrose concentration of ∼40% and layered onto 62% sucrose in polycarbonate centrifuge tubes (Beckman Coulter, 34058). 35% (1.02M) ...
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bioRxiv - Immunology 2023Quote: ... After adding 450 μL of RBC lysis solution (QuantoBio Technology, Beijing, China) and 15 min incubation the samples were analysed using a DxFLEX flow cytometer (Beckman Coulter, USA). All data were analysed using FlowJo X (FlowJo ...
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bioRxiv - Genomics 2020Quote: ... The PCR products were purified by using 1.3X Agencourt AMPure XP beads (2 or 3 cycles; Beckman Coulter) and evaluated by using an Agilent High Sensitivity DNA Kit on an Agilent 2100 Bioanalyzer (Agilent) ...
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bioRxiv - Genomics 2021Quote: ... and PCR reactions were performed with 12 cycles and libraries were purified using Agencourt AMPure XP beads (Beckman). Libraries were sequenced on NextSeq 500 High-Output 25bp PE platform (Illumina).
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bioRxiv - Genomics 2021Quote: ... The ligated products were amplified with 18 cycles of PCR followed by purification using AMPure beads (Beckman Coulter). The enriched libraries were diluted to a final concentration of 5 nM ...
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bioRxiv - Microbiology 2021Quote: ... 27 cycles were used for PCR amplification and cDNA was purified using Agencourt AMPure XP beads (Beckman Coulter) using 15 μl of elution buffer (Takara) ...
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bioRxiv - Microbiology 2020Quote: ... The DNA from these PCR reactions was purified using 90 uL Agencourt AMPure XP beads (Beckman Coulter A63880) before being pooled in equimolar ratios and sequenced using an Illumina MiSeq Paired-end 250-cycle kit.
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bioRxiv - Systems Biology 2020Quote: The barcoding PCR product was cleaned by adding 0.8X (by volume) AMPure XP SPRI magnetic beads (Beckman Coulter) according to manufacturer’s manual and eluted in 38μl DDW ...
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bioRxiv - Genomics 2021Quote: ... The two pools of PCR reactions were each purified with two rounds of AMPure XP beads (Beckman Coulter) at 1.2× sample volume of beads.
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bioRxiv - Genomics 2021Quote: ... the PCR product is pooled and purified with 1x AMPure XP beads to remove primers (Beckman Coulter A63881). Pooling ratios of PCR1 products were empirically determined (Supplemental Figure 4D ...
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bioRxiv - Genomics 2021Quote: ... the PCR product is pooled and purified with 0.8x AMPure XP beads to remove primers (Beckman Coulter A63881). Pooling ratios of PCR1 products were empirically determined (Supplemental Figure 4 ...
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bioRxiv - Genetics 2020Quote: ... Illumina modified adapters added with 10 new PCR cyles after purification with AMPure XP beads (Beckman-Coulter, USA) were ...
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bioRxiv - Immunology 2021Quote: ... PCR products were purified by performing a dual-sided size-selection using SPRIselect magnetic beads (Beckman Coulter #22667) to produce final ...
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bioRxiv - Immunology 2021Quote: ... PCR products were purified by performing a dual-sided size-selection using SPRIselect magnetic beads (Beckman Coulter #22667) to produce final ...
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bioRxiv - Genomics 2020Quote: ... We purified PCR products in a 1:1 ratio using AmpureXP purification beads (Beckman Coulter, High Wycombe, UK), and quantified the purified amplicon using the Qubit dsDNA High Sensitivity assay kit on a Qubit 4.0 instrument (Life Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA fragments were enriched by PCR to obtain barcoded libraries size-selected with AMPureXP beads (Beckman Coulter, #A63881). Quantification and quality control of each library was assessed by using a 2100 Bioanalyzer ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was amplified by PCR and the cDNA product was purified with the SPRIselect Reagent Kit (Beckman Coulter). Sequencing libraries were constructed using the reagents provided in the Chromium Single-Cell 3’ Library Kit following the user guide ...
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bioRxiv - Cell Biology 2023Quote: ... Preparation of PCR reactions was automated by the Echo 525 Acoustic Liquid Handler (Beckman Coulter, Brea, CA, USA). A secondary screen was subsequently performed only on the top 30 performing siRNAs at 6 concentrations (i.e. ...
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bioRxiv - Neuroscience 2023Quote: ... Equal volumes of barcoded PCR products were then pooled and cleaned-up using SPRISelect paramagnetic beads (Beckman Coulter) using a 0.6 × bead/sample ratio ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products were purified with Agencourt AMPure XP SPRI beads according to the manufacturer’s instructions (Beckman Coulter, A63880). Samples were sequenced on a MiSeq (Illumina) ...
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bioRxiv - Genomics 2023Quote: ... The supernatant of the PCR product was purified and dual size-selected using 0.5x and 0.3x volumes of AMPure XP beads (Beckman) and eluted with 13 µl of TE buffer ...
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bioRxiv - Genomics 2023Quote: ... PCR reactions were purified and unused adaptors removed with an equal volume of AMPure XP beads (Beckman, A63880) following the manufacturer’s protocol and eluted in 20 µl Tris pH 7.8 ...
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bioRxiv - Cancer Biology 2022Quote: ... The captured libraries were amplified by PCR for 15 cycles and cleaned up using AMPure beads (Beckman Coulter).
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bioRxiv - Cancer Biology 2022Quote: ... PCR products were purified with Agencourt AMPure XP SPRI beads according to the manufacturer’s instructions (Beckman Coulter, A63880). Samples were sequenced on a HiSeq2500 HighOutput (Illumina ...
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bioRxiv - Genomics 2022Quote: ... The amplified RNA-seq library was purified by immobilization onto AMPure XP PCR purification system (Beckman Coulter, A63881). The library fragments originated from rRNA and mitochondrial rRNA were treated with ZapR v2 and R-Probes according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Adapter-ligated fragments were PCR amplified using indexing primers followed by purification using Agencourt XP beads (Beckman Coulter). The library electropherograms were assessed using an Agilent Bioanalyzer 2100 and Agilent DNA 1000 kit ...
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bioRxiv - Microbiology 2023Quote: ... The PCR amplified dual indexed libraries were cleaned up with Agencourt AMPure XP magnetic beads (Beckman Coulter; A63881), quantified using Qubit Fluorometer 4 (Life Technologies ...
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bioRxiv - Immunology 2024Quote: ... PCR amplicons were purified by size selection (0.5x followed by 1.2x) using AMPure XP Reagent (Beckman Coulter #A63881), quantified using a Qubit Flex Fluorometer (ThermoFisher #Q3327 ...
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bioRxiv - Molecular Biology 2024Quote: ... Post-PCR size-selection and clean-up was performed by adding 0.9x AMPure XP beads (A63880, Beckman Coulter) to the amplified libraries ...
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bioRxiv - Synthetic Biology 2024Quote: ... The KAPA-PCR products were cleaned using Agencourt® AMPure® XP magnetic beads (Beckman Coulter, Cat. # A63881) and 200 ng of this product was used as template for a second PCR with KAPA Ready Mix to introduce Illumina Barcodes through 10 PCR cycles followed by cleanup using magnetic beads as mentioned before ...
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bioRxiv - Cancer Biology 2024Quote: ... Post-PCR clean-up was performed by adding 1.3× volume (65 µl) of Ampure XP beads (Beckman Counter) to the PCR reaction (50 µl ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were purified using AMPure XP beads at a 0.6 ratio (Beckman Coulter Inc. Brea, CA, USA). Amplicons were prepared for sequencing using a native barcoding kit (Oxford Nanopore SQK-NBK114.96 ...
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bioRxiv - Molecular Biology 2024Quote: ... using 12 PCR cylces following manufacturer’s instruction (except for the use of Ampure XP beads (Beckman Coulter, #A63880) instead of SRPIselect beads) ...
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bioRxiv - Molecular Biology 2024Quote: ... The barcoded PCR products were further purified with 0.4x volume of AMPure XP Beads (Beckman Coulter, California, USA). All barcoded PCR products were pooled in equal amounts to prepare 1 µg of DNA in 50 µL nuclease-free water for library preparation ...
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bioRxiv - Neuroscience 2024Quote: ... to index the clean PCR products and repurified them with AMPure XP magnetic beads (Beckman Coulter, Chaska, MN). Each 50 μL index PCR reaction contained 5 μL 10X AccuPrime PCR Buffer II ...
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bioRxiv - Bioengineering 2024Quote: ... Purification of PCR amplicons are carried out using AMPure XP bead-based reagent (Beckman Coulter Cat. No. A63882) according to manufacturer’s protocol ...