Labshake search
Citations for Beckman :
851 - 900 of 944 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... sgRNA amplification was performed using a two-step PCR strategy and purified using Agencourt AMPure XP (A63880; Beckman Coulter UK), Illumina sequencing (19-bp single-end sequencing with custom primers on the HiSeq2500 Rapid Run ...
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bioRxiv - Neuroscience 2020Quote: ... PCR products were then cleaned up by double-sized selection by using Ampure beads (Agencout AMPure XP, Beckman Coulter, #A63880) to remove large and small DNA fragments ...
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bioRxiv - Immunology 2021Quote: ... The first PCR reaction was purified using the AMPure Size Select Magnetic Bead Kit at a ratio of 0.6X (Beckman Coulter). Amplicon libraries were then prepared according to the Pacific Biosciences Multiplex SMRT Sequencing protocol and sequenced on a Pacific Biosciences Sequel platform (Pacific Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were confirmed through visualization in 1% agarose gel and purified using AMPure XP beads (Beckman Coulter, Indianapolis, U.S.A.). PCR libraries were pooled together and sent to the Centre d’expertise et de services Genome Québec (Montréal ...
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bioRxiv - Microbiology 2022Quote: ... PCR products from the same samples were then pooled and cleaned using AMPure XP beads (Beckman Coulter, Pasadena, CA, USA). Concentration of PCR products was assessed using Qubit technology (Life Technologies ...
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bioRxiv - Plant Biology 2024Quote: The replicates of the second PCR were pooled for each sample and purified with Agencourt AMPure XP beads (Beckman Coulter) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR products of 100−1,000 bp (including adaptor and primer sequences) were purified using AMPure XP beads (Beckman Coulter) and captured on an Illumina flow cell ...
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bioRxiv - Molecular Biology 2024Quote: ... The initial PCR products of rbcL F3R3 were purified using an Agencourt AMPure XP kit (Beckman Coulter, Fullerton, CA, USA). To construct the DNA libraries for the second PCR ...
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bioRxiv - Cancer Biology 2024Quote: ... Each ATAC library was amplified with Nextera primers for 16 PCR cycles and purified with Agencourt AMPure XP (Beckman Coulter) to remove excess primers ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The P2-ligated DNA was then amplified for 12 rounds of PCR using custom RAD primers (Hohenlohe et al., 2012) and cleaned with 0.8X AMPure XP beads (Beckman Coulter). The final libraries were sequenced either on an Illumina HiSeq 4000 or an Illumina NovaSeq 6000 to generate 2×150 bp reads (Table S3).
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bioRxiv - Microbiology 2023Quote: ... This PCR amplification was followed by another purification using the Agencourt® AMPure® XP beads (#A63881, Beckman Coulter, UK). A ratio of 6.5 of Solid Phase Reversible Immobilisation beads (SPRI ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were pooled and purified using size-selective SPRIselect magnetic beads (0.8 left-sized, Beckman Coulter, Brea, CA, USA). The library was then diluted to 6pM for sequencing ...
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bioRxiv - Biochemistry 2023Quote: ... Eight 50 μL PCR reactions were combined in a 1.5 mL Eppendorf tube and mixed with 320 μL Ampure XP beads (Beckman Coulter) (0.8:1 ratio ...
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bioRxiv - Immunology 2023Quote: ... the PCR plate was briefly centrifuged and 25 µl of room-temperature AMPure XP beads (1:1 ratio; Beckman Coulter) was added to each well and incubated for 8 minutes at room temperature ...
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bioRxiv - Genetics 2023Quote: ... Amplicon PCR products were then purified by a 0.65X + 1.05X double size selection with AMPure XP Beads (Beckman Coulter, A63882). Purified amplicons were then pooled based on the concentrations measured by the Quant-iT™ dsDNA Assay HS Kit (Thermo Fisher ...
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bioRxiv - Evolutionary Biology 2023Quote: ... viral cDNA was purified either by standard PCR reaction desalting columns or with a 0.45X volume of AMPure XP beads (Beckman Coulter). Nextera XT libraries were generated with 1 ng of cDNA material ...
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bioRxiv - Genomics 2024Quote: ... Post-PCR DNA cleanup was performed by adding 50 µL (1x volume) of AMPure XP bead slurry (Beckman Coulter, A63882) and incubating for 10 min at RT ...
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bioRxiv - Evolutionary Biology 2024Quote: Barcoded PCR products were pooled from triplicate pools and purified by double-sided size selection using Ampure beads (Beckman Coulter). Large DNA was first removed by incubation with 0.8x bead volume and magnetization of beads ...
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bioRxiv - Microbiology 2024Quote: ... viral cDNA was purified either by standard PCR reaction desalting columns or with a 0.45X volume of AMPure XP beads (Beckman Coulter). Libraries were generated either by Nextera XT with 1 ng of cDNA material ...
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bioRxiv - Microbiology 2021Quote: ... 2.5 ml of each solution were used to form the gradient in a 14 ml Ultra-Clear™ centrifuge tube (Beckman Coulter, Brea, CA, USA), with the highest sucrose concentration at the bottom of the tube ...
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bioRxiv - Evolutionary Biology 2024Quote: ... were then added to the tubes and then solutions were allowed to stand for 20 min and the optical absorbance at 620nm was measured with DU800 spectrophotometer (Beckman Coulter Inc., Brea, CA, USA). The malachite green-ammonium molybdate solution was prepared as described by Onodera et ...
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bioRxiv - Neuroscience 2021Quote: ... 12 cycles of PCR were performed for library amplification and libraries were cleaned and size selected (Ampure XP beads; A63880, Beckman Coulter) as described (Sanz and Chédin ...
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bioRxiv - Cell Biology 2022Quote: ... The cDNA amplification from single cells was performed by PCR for 21 cycles and the amplified products were purified using AMPure XP paramagnetic beads (Beckman Coulter).
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bioRxiv - Genomics 2020Quote: ... Equal quantities of the four PCR products from each mosquito pool were mixed and purified using Agencourt AMPure XP (Beckman Coulter).
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bioRxiv - Neuroscience 2020Quote: ... A total of 25 μl of the PCR product was cleaned using 1X Agencourt AMPure XP beads (Beckman Coulter, Brea, USA) and eluted in 25 μl TE buffer ...
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bioRxiv - Cell Biology 2020Quote: ... The first PCR reaction was performed for 15 cycles with 60 °C annealing and 30 s elongation and then purified with AMPure XP PCR beads (Beckman Coulter). The second PCR was performed for 15 cycles for wild type-specific and for 21 cycles for tag-specific amplicons respectively using 60°C annealing and 30 s elongation ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were purified using 1.8X Agencourt AMPure XP PCR Purification beads according to the manufacturer’s instructions (Beckman Coulter, cat no A63881). The sheared DNA samples were quantified and qualified on a BioAnalyzer system using the DNA7500 assay kit (Agilent Technologies cat no ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were tagged using barcodes NB01 to NB05 of the Native Barcoding Expansion 1-12 PCR-free kit (EXP-NBD104, ONT) and purified using Agencourt AMPure XP beads (Beckman Coulter). The barcoded samples were quantified using a Qubit fluorometer and pooled equimolarly to reach 700 ng in 65 μL ...
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bioRxiv - Molecular Biology 2020Quote: ... The library was amplified with 9 PCR cycles and cleaned with 0.9x AMPure® XP beads (A63881, Beckman Coulter, CA, USA) to enrich for DNA fragments shorter than 50nt ...
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bioRxiv - Molecular Biology 2020Quote: ... The target library derived from the oocyte RNA polymerase II transcripts was PCR-amplified (15 cycles for P14 WT, 16 cycles for P14 Tbpl2−/− mutant) and purified using AMPure beads (Beckman Coulter) to remove short PCR artifacts (< 200bp ...
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bioRxiv - Molecular Biology 2021Quote: ... Indexing PCR amplicons were pooled (4◻μl of each) and purified using AMPure XP beads (Beckman Coulter, California, United States) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: The obtained PCR products were purified and filtered by using AMPure XP magnetic beads with double size selection (Beckman Coulter, Inc.). In brief ...
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bioRxiv - Genetics 2020Quote: ... PCR was then analyzed on an agarose gel and DNA was cleaned up using Ampure XP beads (Beckman Coulter, cat. A63881). For this the four PCR reactions were pooled resulting in 100 μL ...
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bioRxiv - Molecular Biology 2020Quote: ... The amplified DNA was purified using a Qiagen MinElute PCR Purification Kit and primer dimers (<100 bp) were removed using AMPure beads (Beckman Coulter). ATAC-Seq was performed with two biological repeats to ensure the robustness of the data sets ...
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bioRxiv - Immunology 2021Quote: ... The 1st-round PCR product was cleaned up and followed by a double size selection step using SPRIselect reagent (Beckman Coulter) to separate protein library from cDNA library.
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bioRxiv - Neuroscience 2021Quote: ... 50 μL of PCR product from the pre-amplification step were purified using 50 μL of Ampure XP beads (Beckman Coulter), resuspended ...
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bioRxiv - Developmental Biology 2021Quote: ... PCRs were monitored by qPCR to avoid over-ampliciation and following every PCR reaction the samples were purified using SPRI beads (Beckman Coulter) and libraries were sequenced at 1:10 proportion of the transcriptome library on Illumina’s NovaSeq.
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bioRxiv - Genomics 2020Quote: ... We carried out the first round of PCR for 3 cycles and purified the reactions with Ampure XP beads (Beckman-Coulter). We then carried out a second round of PCR with primers targeting the P5 and P7 sequences only ...
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bioRxiv - Microbiology 2021Quote: ... with a final extension at 72 °C for 10 min PCR products were purified using Agencourt AMPure XP beads (Beckman Coulter) ratio 0.6× ...
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bioRxiv - Microbiology 2020Quote: ... We pooled the triplicate PCR products and the 24 μL of final volume were purified by adding 44 μL of AMPure XP beads (Beckman Coulter), mixing with pipette followed by a 5 minute incubation ...
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bioRxiv - Immunology 2020Quote: ... 2) first-round PCRs were set up using a Biomek FX liquid handling system with a 96- well head (Beckman Coulter); 3 ...
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bioRxiv - Genomics 2020Quote: ... These pooled single-cell libraries were used in an AMpure XP Bead-based Dual Bead Cleanup and Size Selection reaction (Agencourt AMPure XP PCR Purification modified Instructions for Use, Beckman Coulter). In both bead cleanup reactions ...
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bioRxiv - Molecular Biology 2023Quote: ... Size selection purifications following the ligation and amplification PCR steps were performed with 0.9× reaction volumes of Agencourt AMPure XP beads (Beckman Coulter A63880). Purified libraries were combined as an 8-sample equimolar pool containing a combination of indexes from 1–12 and sequenced on an Illumina NextSeq on an Illumina NextSeq 2000 on a P2 flow cell (paired-end 75-bp reads).
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bioRxiv - Microbiology 2024Quote: ... The PCR products from thew first PCR were individually purified using 0.6X volumes of AMPure XP magnetic beads (AMPure XP beads kit / Beckman coulter, USA), followed by a second PCR reaction to attach the dual indices (S5 and N7 ...
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bioRxiv - Immunology 2023Quote: ... Final PCR reaction was then purified with a MinElute PCR Purification Kit followed by size-selection (150bp-1000bp using Ampure XP beads, Beckman Coulter). Sequencing was performed using a MGISEQ-2000 (BGI Tech Solutions) ...
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bioRxiv - Genomics 2024Quote: ... c) increasing PCR cycles (15 in total) and eliminating primer dimers prior to sequencing (Agencourt AMPure XP magnetic beads, Beckman Coulter). Primary PDAC were sequenced on the NovaSeq6000 ...
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Reduction of chromosomal instability and inflammation is a common aspect of adaptation to aneuploidybioRxiv - Cell Biology 2023Quote: ... Samples were purified using 1.8X Agencourt AMPure XP PCR Purification beads according to the manufacturer’s instructions (Beckman Coulter, cat no A63881). The sheared DNA samples were quantified and qualified on a BioAnalyzer system using the DNA7500 assay kit (Agilent Technologies cat no ...
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bioRxiv - Genomics 2024Quote: ... the PCR product was cleaned up and followed by the 10X Genomics double size selection step using SPRIselect reagent (Beckman Coulter) to separate the protein library (2X SPRI ...
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bioRxiv - Genomics 2023Quote: PCR reaction products were cleaned and size selected using a 0.7:1 ratio of AMPure XP SPRI beads (Beckman Coulter Inc.) to sample ...
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bioRxiv - Genomics 2023Quote: ... The PCR amplification is followed by a bead clean up step with 1X sample-to-bead ratio AMPure XP beads (Beckman Coulter), binding at room temperature for 5 min ...