Labshake search
Citations for Beckman :
651 - 700 of 944 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... PCR cleanup was performed after PCR1 as well as PCR2 by AMPure XP beads (Beckman Coulter). The concentration and purity of final PCR products was determined by Qubit and Bioanalyzer2100.
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bioRxiv - Genetics 2024Quote: ... 18μL PCR product was purified with an equal volume of AMPure XP beads (Beckman Coulter, A63880). The DNA was eluted with 52.5 μL of 10 mM Tris (pH 8.5 ...
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bioRxiv - Immunology 2024Quote: ... A fraction of each sample’s pooled PCR product was cleaned using AMPure XP beads (Beckman Coulter), then quantified by qubit and diluted to 4nM ...
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bioRxiv - Genetics 2023Quote: ... Nested PCR amplicons were purified using 1.12 × AMPure XP beads (#A63881; Beckman Coulter Genomics, Brea, CA). A 10% spike-in of PhiX control V3 (#FC-110-3001 ...
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bioRxiv - Genetics 2023Quote: ... The gel-extracted DNA was PCR-amplified and purified using AMPure XP Beads (Beckman Coulter A63880) with a 0.9x bead cleanup ratio ...
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bioRxiv - Developmental Biology 2023Quote: ... Surplus PCR primers were further removed by purification using AMPure XP beads (Beckman-Coulter, Villepinte, France) and the final cDNA libraries were checked for quality and quantified using capillary electrophoresis ...
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bioRxiv - Pathology 2023Quote: ... Each subpool of PCR products was purified with two rounds of AMPure XP beads (Beckman Coulter) at 1.2× sample volume of beads.
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bioRxiv - Microbiology 2023Quote: ... the PCR products were cleaned using AMPure XP binding beads (Beckman Coulter Genomic, Indianapolis, IN, USA). The AMPure XP binding beads (Beckman Coulter Genomic ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR products were purified using a 1.8X volume of AMPure XP beads (Beckman Coulter, CA, USA) and submitted for Sanger sequenced (Azenta Genewiz ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries amplified in 14 PCR cycles were purified using Agencourt AMPure XP beads (Beckman-Coulter, USA), at a ratio 0.9x to remove adapter dimer contamination ...
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bioRxiv - Molecular Biology 2022Quote: ... the second PCR products were again purified with magnetic beads (Agencourt AMPure XP® – Beckman Coulter), quantified using NanoDrop® 2000 (Thermo Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... PCR reactions were purified with 0.7X AMPure XP beads (Agencourt AMPure XP beads; Beckman, Cat.no. A63881), quantified with Quant-it High Sensitivity Kit (Q33120 ...
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bioRxiv - Cell Biology 2022Quote: ... The PCR primers were removed with 1x 0.9:1 SPRI beads (Beckman Coulter, Cat no. A63880) according to manufacturer’s instructions and samples eluted in 20μl ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was amplified using PCR Primer II A and subsequently purified using Ampure XP beads (Beckman). Illumina libraries were prepared using Nextera XT DNA library preparation kits (Illumina ...
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bioRxiv - Genetics 2023Quote: ... Nested PCR amplicons were purified using 1.12X AMPure XP beads (Beckman Coulter Genomics, Brea, CA, USA). Ten percent spike-in of PhiX control V3 (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... and purification of the PCR products was completed using AmpureXP magnetic beads (Beckman Coulter, CA, USA) following manufacturer protocols ...
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bioRxiv - Developmental Biology 2024Quote: ... the clean-up of PCR product was carried out by using AMPure X beads (Beckman Coulter) (in a ratio of 0.9:1) ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR products were cleaned up by using Agencourt AMPure XP beads (1.4X volume; Beckman Coulter) and sequenced on an Illumina NextSeq 500 to obtain 2 × 75 nt pairedend reads or on an Illumina NovaSeq 6000 to obtain 2 × 150 nt paired-end reads at the Genome Sequence and Analysis Facility of the University of Texas at Austin.
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bioRxiv - Plant Biology 2024Quote: ... The amplified PCR products were purified with Agencourt AMPure beads (Beckman Coulter, Brea, CA, United States). Illumina libraries were constructed using the Ovation Rapid DR Multiplex System 1-96 (NuGEN Technologies ...
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bioRxiv - Microbiology 2024Quote: ... The digested DNA fragments were purified using the Agencourt AMPure XP PCR Purification Kit (Beckman Coulter) and sent to GENEWIZ for fragment analysis ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were amplified for 11 PCR cycles and size selected using SPRI selected beads (Beckman Coulter).
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bioRxiv - Neuroscience 2024Quote: ... The resulting PCR products were purified through double-sided AMPure XP beads cleanup (Beckman Coulter, A63882), followed by bioanalyzer quality control to ensure the obtainment of clean PCR products with appropriate size ...
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bioRxiv - Genomics 2024Quote: ... We purified the amplified PCR product with 1.8x volumes of AMPureXP SPRI beads (Beckman Coulter, A63881). We pooled libraries based on molar concentrations and sequenced on a NextSeq 500 instrument (Illumina ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR products were pooled and purified using 1.2x AMPure XP beads (Beckman Coulter, Cat. No. A63880). Sequence flow cell adapters and dual sample indices were then appended in a second PCR reaction (cycling conditions ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR products were pooled and purified using 1.2x AMPure XP beads (Beckman Coulter, Cat. No. A63880). Sequence flow cell adapters and dual sample indices were then appended in the second PCR reaction using Q5 polymerase (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... The PCR-products were purified with 20 µl AMPure XP beads (Beckman Coulter Genomics, Cat: #A63881) before performing the second PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... The gradient was formed by adding 3 mL of the iodixanol solutions to a 17 mL polypropylene tube (Beckman Coulter). Ultracentrifugation was performed using a SW Type 32.1 Ti rotor in Optima XPN-100 Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Bioengineering 2020Quote: ... the EV pellets were resuspended in 3 ml 0.32 M sucrose (diluted in PBS) solution and centrifuged again at 100,000xg for 60 minutes at 4°C (SW60 rotor, Beckman Coulter). Sucrose supernatant was removed and pellet was resuspended in 500 μl phosphate-buffered saline containing 0.01% bovine serum albumin (0.2 μm filtered ...
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bioRxiv - Cell Biology 2020Quote: 5-20% sucrose gradients were prepared on a Biocomp Gradient Station IP from 5% and 20% solutions of sucrose in gradient buffer (50mM Tris HCl pH7.5, 150mM NaCl, 1mM EDTA) to Seton tubes (Beckman Coulter). Single cell suspensions of approximately 3×108 cells from thymus and bone marrow were obtained as above ...
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bioRxiv - Microbiology 2020Quote: ... parkeri was overlaid on a 30% MD-76R (Merry X-Ray) gradient solution in ultracentrifuge tubes (Beckman/Coulter Cat 344058). Gradients were centrifuged at 18,000 r.p.m ...
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bioRxiv - Genomics 2020Quote: ... the beads were removed from the solution using a magnet and DNA was purified using 1.8× volume of Agencourt AMPure XP (Beckman Coulter) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: LCM samples were washed from lysis buffer by mixing with an equal portion of SPRI bead solution (Beckman Coulter, A63881), incubated 5 min R/T and washed twice with 100% EtOH under magnetic field ...
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bioRxiv - Plant Biology 2023Quote: ... and all granules purified within this fraction was resuspended in known volumes of Isoton II electrolyte solution (Beckman Coulter, Indianapolis). The suspension was analysed in a Multisizer 4e Coulter counter (Beckman Coulter ...
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bioRxiv - Neuroscience 2024Quote: ... the pellet was suspended in 250 μl of cold FACS buffer and the resulting solution was finally sorted on a MoFloAstrios device (Beckman) to reach a concentration of 410 cells/μl.
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bioRxiv - Developmental Biology 2023Quote: ... The top 1 mL was loaded onto a 52% isotonic Percoll solution and centrifuged at 27,000 × g for 10 min at 10°C (Optima-XPN-80, Beckman Coulter). Following centrifugation ...
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bioRxiv - Microbiology 2023Quote: ... and 5.5 ml was layered on top of a two-step sucrose gradient consisting of 5 ml of 40% sucrose solution layered on top of 1.5 ml of a 65% sucrose solution in a 14 x 89mm Ultra-Clear tube (Beckman Coulter). All sucrose (w/w ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 200µl BD 1X Perm/Wash solution and stained 1h at 4°C with anti-HIV-1 p24 (RD1/PE, Beckman Coulter #6604667 ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysates were sheared with a 25-gauge 5/8 needle and loaded onto CsCl solution (150% w/v) for ultracentrifugation in NVT 65.2 rotor (Beckman coulter) at 42,000 rpm for 20 hours at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... step gradients were made by superposing sucrose solutions of different concentration (50%, 40%, 30%, 20%, 10%) in an ultra-clear polycarbonate tube (Beckman), and a linear gradient was allowed to form overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mL samples were supplemented with 500 uL of Optiprep to yield a 20% iodixanol solution and underlayered beneath 1.5 mL layers of 9% and 12% Optiprep/PBS solutions in a 4.9 mL OptiSeal tube (Beckman-Coulter, 362185) using a disposable glass cannula ...
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bioRxiv - Biochemistry 2023Quote: ... The reaction mixture was applied onto the top of the gradient solution and was centrifuged at 41,000 rpm at 4 °C for 17 hrs using an SW41 Ti rotor (Beckman Coulter). The gradient was fractionated using a Piston Gradient Fractionator (BioComp) ...
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bioRxiv - Microbiology 2023Quote: ... Carbon source stock solutions were added to microplates using a Biomek FxP liquid handling robot (Beckman Coulter, Indianapolis, IN, USA) and kept in an anaerobic chamber (Coy ...
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bioRxiv - Biochemistry 2024Quote: ... and the solution was centrifuged for 45 min at 20,000g at 1 °C (Beckman Coulter J2-21, JA-20 rotor). The supernatant was then loaded onto a 5 ml HisTrap column (Cytiva ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and fragments between 400-500 bp were selected for purification using a 25X AMPure beads solution (Beckman Coulter Inc, Indianapolis, IN). Fragments were then amplified with a NEBNext Ultra Q5 PCR protocol ...
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bioRxiv - Systems Biology 2020Quote: ... and 1 mL of 5% (w/v) iodixanol solutions on top of each other in a 5 mL open top polyallomer tube (Beckman Coulter). Crude exosomes were resuspended in 200 µL PBS and overlaid on top of the gradient ...
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bioRxiv - Genomics 2021Quote: ... Cells were washed with 25 mm supplemented HBSS before depleting the sample of unnucleated cells using IOTest 3 Lysing solution (Beckman Coulter) following provider instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Sample solution containing EVs was placed at the top of the tube (at RT) and ultracentrifugation (Beckman Optima L-90K Ultracentrifuge) was performed at 40,000 RPM for 16 hours at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... 750 µL of the 80% Histodenz solution were added and thoroughly mixed with 750µL of the protein-LUV sample in Thinwall centrifuge tubes (Beckman 50.2ti rotor). 1.5 mL of the 30% Histodenz solution was added as the intermediate layer ...
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bioRxiv - Cell Biology 2022Quote: ... which was then slowly poured on top of 10 ml cushion solution (2.05M sucrose with protease inhibitors, DTT and PMSF) in the ultracentrifugation tube (Beckman polyallomer #326823). After a 45-min spin at 24,600 rpm in pre-chilled SW32Ti rotor at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... the homogenized solution was added onto a sucrose cushion (2 ml) in a 10 ml Ultra-Clear centrifuge tube (Beckman Coulter). Separation of nuclei from the tissue was done by centrifugation (26.500xg at 4°C for 1.5 h ...