Labshake search
Citations for Beckman :
601 - 650 of 1639 citations for 1 4 Nitrophenyl cyclobutanecarbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... supernatant was centrifuged for 3 h at 115,000 g and 4°C using the XPN-80 ultracentrifuge equipped with a Ti70 rotor (Beckman). EV pellets were resolved in 1 mL ice-cold PBS (Gibco ...
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bioRxiv - Plant Biology 2023Quote: ... Extract was then centrifuged at 40 000 rpm for 2 hours at 4°C using the 50Ti rotor (Beckman). A protocole previsouly used in the laboratory and described in Maclot et al ...
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bioRxiv - Microbiology 2022Quote: ... Glycoproteins in the supernatants were pelleted by centrifugation at 100,000×g at 4°C for 90 min using a TLA-55 rotor (Beckman). The resultant pellets ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation (183,960 x g, 88 min, 4°C) in a Type 70Ti rotor (Beckman). The supernatant was mixed with 2 mL of IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified via centrifugation (66,000 x g, 30 min, 4°C) in a Type 70 Ti rotor (Beckman). The supernatant was mixed with 1.5 mL of anti-FLAG M2 affinity gel (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... then samples were centrifuged at 2,700 RPM for 10 minutes at 4°C in a tabletop centrifuge (Beckman Coulter). The supernatant (serum ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 200,000 x g for 2.5 h (high g) at 4°C in a TLA-100.3 fixed angle rotor (k-factor 14) (Beckman Coulter) and the pellet was resuspended in 25 ∝l lysis buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Gradients were spun at 45,000 RPM for 16 hours at 4°C in SW55Ti rotor using an ultracentrifuge (Beckman). After centrifugation ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was then clarified (using a JA 25.50 Beckman rotor at 40000x g for 30 min, 4°C) and the soluble fraction was incubated with 2 ml of Glutathione Sepharose 4B resin for 2h at 4°C under constant agitation ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 μg/mL cycloheximide if reaction contained cycloheximide) for 3.5 hrs at 240,000 g at 4 °C using a SW41 rotor (Beckman Coulter). Gradients were fractionated using Teledyne Isco Tris Peristaltic Pump and fractions were collected and pooled according to the UV trace ...
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bioRxiv - Biochemistry 2023Quote: ... Density gradient centrifugation was done at 50,000 rpm for two hours at 4 °C using a 70.1 Ti rotor (Beckman Coulter). Post centrifugation ...
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bioRxiv - Immunology 2023Quote: ... and then centrifuged at 260,800 x g for 2.5 h at 4°C in an Optima L-100XP ultracentrifuge (Beckman–Coulter) using the SW41Ti rotor with brake ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 200,000 x g for 2.5 h (high g) at 4°C in a TLA-100.3 fixed angle rotor (k-factor 14) (Beckman Coulter) and Red-C12 fluorescence of the starting material ...
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bioRxiv - Cell Biology 2024Quote: ... The lysate was clarified by centrifugation (66,000 x g, 30 minutes, 4 °C) in a Type 70Ti rotor (Beckman). For His-ZZ-TEV-Halo-CCSer2650–850 ...
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bioRxiv - Cell Biology 2024Quote: ... 0.1 mM PMSF buffer and subjected to ultracentrifugation at 36,000 rpm at 4°C for 2h using a SW41 rotor (Beckman) and 14 x 89 mm polypropylene tubes (Beckman ...
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bioRxiv - Microbiology 2024Quote: ... Samples within each biological replicate were centrifuged simultaneously for 2.5 h at 209,490 x g and 4°C using the SW41Ti rotor in an LE-80 ultracentrifuge (Beckman). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... Solutions of 15% and 23% Optiprep were layered on top or below the sample and centrifuged at 4°C for 16 h at 120,000g in a SW 28 Ti swinging bucket rotor (Beckman).
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bioRxiv - Cell Biology 2024Quote: ... The gradients were centrifuged at 39,000 rpm for 2.5 h at 4°C using a SW41Ti rotor (Beckman Instruments), and 21 fractions of 0.5 ml were collected ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was passed through a 0.22 μm filter before undergoing ultracentrifugation at 100,000g for 70 min at 4°C (Optima-XE SW41 Beckman Coulter). The resulting pellet was then resuspended in 2 ml of 0.475 M sucrose solution prepared in double-filtered PBS ...
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bioRxiv - Microbiology 2024Quote: ... the supernatants were loaded onto 1 mL of 30% sucrose in PBS buffer and centrifuged at 25,000 rpm at 4°C for 3 hours using the 90AT rotor (Beckman). The pelleted viral particles were resuspended in 100 μL of PBS and fixed with formaldehyde (working concentration 0.1% ...
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bioRxiv - Molecular Biology 2024Quote: ... Proliferation was measured after 4-5 days either by trypan-blue mediated count (Vi-cell blu counter, Beckman Coulter), or by adding the Prestoblue dye (Life Technologies ...
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bioRxiv - Microbiology 2024Quote: ... were centrifuged at approximately 108,000 x g for 107 min at 4°C using an ultracentrifuge device equipped with a TLA 100.3 fixed-angle rotor (Beckman). The supernatants were retained as soluble fractions and kept at 4°C for downstream analyses ...
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bioRxiv - Immunology 2024Quote: ... Sartorus) at 100,000 x g for 70 min at 4°C using the ultracentrifuge Optima MAX-TL (Beckman Coulter). The pellets were suspended in PBS(– ...
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bioRxiv - Cell Biology 2024Quote: ... The lysate was clarified via centrifugation (66,000 x g, 30 minutes, 4 °C) in a Type 70 Ti rotor (Beckman). The supernatant was mixed with 1.5 mL of α-FLAG M2 affinity gel (Sigma–Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... The lysate was clarified by centrifugation (183,960 x g, 88 minutes, 4 °C) in a Type 70Ti rotor (Beckman). The supernatant was mixed with 2 mL of IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Biochemistry 2024Quote: ... The unbroken cells and debris were removed by centrifugation at 20,000 x g for 10 min at 4 °C (Beckman Coulter Avanti J20 XPI centrifuge ...
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bioRxiv - Biochemistry 2024Quote: ... dimethylammonio)-1-propanesulfonate)] and lysates were cleared by centrifugation at 75,600 g at 4 °C for 30 min using a JA 25.50 rotor (Beckman-Coulter). Next ...
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bioRxiv - Neuroscience 2024Quote: ... EV-depleted FBS medium was obtained by the ultracentrifugation of BV-2 complete medium supplemented with 20% FBS for 18h at 100.000 × g and 4°C in 38.5 mL polyallomer Ultra Clear ultracentrifuge tubes (Beckman Coulter), using an Optima XPN-80 ultracentrifuge and an SW 32 Ti Rotor (Beckman Coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... The samples were ultracentrifuged at 100,000 × g for 135 min at 4°C (MLA-55 rotor, Beckman Coulter, USA). After removal of the supernatant ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... containing cell nucleus and other heavy organelles was discarded and supernatant was centrifuged for 30 min at 48,000 × g at 4 °C (Beckman Avanti J-251 Ultra-centrifuge ...
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bioRxiv - Neuroscience 2024Quote: ... Tubes were centrifugated at 100,000 x g (32,500 RPM) for 3 hours at 4°C using a SW55Ti rotor (Beckman Coulter). Synaptosomes enriched in the interface between 1.0-1.25 M sucrose were collected and diluted with ice-cold 0.1 mM CaCl2 ...
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bioRxiv - Cell Biology 2024Quote: ... and the membranes were pelleted at 100,000 g for one hour at 4°C in a TLA 100.3 rotor (Beckman-Coulter). Afterwards the supernatant was taken off and stored at −20°C (“S100” ...
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bioRxiv - Biophysics 2024Quote: ... Lysates were clarified by ultracentrifugation at 34,000 rpm for 45 minutes at 4°C in a Type 45.1 Ti rotor (Beckman-Coulter). The supernatants were then passed through a 0.22 μm syringe sterile filter (Thermo-Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... were layered on top of each sucrose gradient and centrifuged (41,000 rpm for 2 hours at 4°C) in an SW41 Ti rotor (Beckman). UV profiling (254 nm ...
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bioRxiv - Neuroscience 2024Quote: ... The homogenate was layered on the gradient and centrifuged (3h, 4°C, 160,000 xg) using a SW40 rotor (Beckman). Top layer of debris was removed and ∼18 fractions of 500 µl were collected from each gradient from top (fraction 1 ...
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bioRxiv - Biophysics 2024Quote: Tau proteins were diluted in cold 1x BRB80 to a concentration of a few tenths of micromolar and centrifuged to remove aggregates (10 min/4°C /215,000 x g in Type 70 Ti rotor [Beckman]). Prior to use ...
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bioRxiv - Biophysics 2024Quote: ... The lysate was clarified via centrifugation (66,000 x g, 30 min, 4°C) in a Type 70 Ti rotor (Beckman), before being incubated overnight at 4°C with 1.5 mL of anti-FLAG M2 affinity gel (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... The crude membrane fraction containing MsbA was isolated by ultracentrifugation (100000g, 45 min, 4°C, Beckman Coulter rotor Ti70). About 2 mg of MsbA-enriched membranes were solubilized with 1% DDM (w/v ...
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bioRxiv - Biophysics 2024Quote: ... Lysis was accomplished via douncing and the lysate was clarified via centrifugation (183,960 x g, 88 min, 4°C) in a Type 70Ti rotor (Beckman). 2 mL of IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Bioengineering 2024Quote: ... for 15 minutes and centrifuged at 10,000 x g for 25 minutes at 4°C (JA20 rotor, Beckman Coulter). The supernatant containing the recombinant proteins was filtered through a 0.45 µm sterile filter (Whatman Puradisc) ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were centrifuged at 24,000 rpm under vacuum for 1.5 hrs at 4°C (Allegra X-15R Centrifuge, Beckman Coulter). The viral pellet was resuspended in 300 µL PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... which was then centrifuged at 100,000 × g for 18 hours at 4°C (344060, SW 40 Ti, Beckman Coulter) for further purification ...
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bioRxiv - Cell Biology 2024Quote: ... and the lysate was cleared twice by centrifugation at 40,000 rcf for 20 min each at 4°C using a JA 25.50 rotor (Beckman Coulter). The cleared lysate was incubated with 2 mL Ni-NTA resin (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... 36 mL of media per condition was subject to ultracentrifugation at 100,000 x g for 6 hours at 4 °C in a SW-32-Ti swinging bucket rotor (Beckman). The media was decanted and 500 μL PBS was added to the pellet and left to resuspend overnight at 4 °C ...
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bioRxiv - Bioengineering 2024Quote: ... the suspension was centrifuged at 10,000 RPM for 10 min at first and 30,000 RPM for 4 hours (Optima XPN-80, Beckman Coulter), and the supernatant was collected to remove the precipitates ...
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bioRxiv - Bioengineering 2024Quote: ... The virus was then pelleted by centrifugation at 4°C for 45 min at 25,000 rpm in a sw28 rotor (Beckman) and gently suspended in dilution buffer (150 mM NaCl ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were loaded based on equal total RNA onto a 10%–50% sucrose gradient and subjected to ultracentrifugation (187,813 g for 1.5 hours at 4 °C) using a SW 41 Ti rotor (Beckman Coulter). Subsequently ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Ribosomes were pelleted by centrifugation at 27,000 rpm (57,000 xg) for 16 h at 4 °C in a Ti-45 rotor (Beckman-Coulter). Ribosomes pellets were then resuspended in dissociation buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... All ultracentrifugation steps will be performed at 4°C using a SW41 Ti rotor (Beckman Coulter, Brea, CA, USA).
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bioRxiv - Cell Biology 2024Quote: ... Cells were harvested at day 3 post-infection by centrifuging at 2,000xg for 15 mins at 4°C using a JLA 8.1000 rotor (Beckman Coulter). Insect cell pellets were frozen in liquid nitrogen for storage or used immediately for protein purification.