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Citations for Beckman :
501 - 550 of 1639 citations for 1 4 Nitrophenyl cyclobutanecarbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... supernatants were harvested and filtered through a 0.45μm filter and centrifuged at 27,000 rpm for 3h at 4°C with a SW41 rotor and Optima L-90 centrifuge (Beckman). Pellets were resuspended in PBS prior to use for western blot analysis.
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bioRxiv - Microbiology 2020Quote: ... The gradient was centrifuged for 17 h at 4°C and 100,000 rcf (23,700 rpm) using an SW 40 Ti rotor (Beckman Coulter), followed by manual fractionation into 20 590 μl fractions and measurement of the A260 nm of each fraction ...
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bioRxiv - Plant Biology 2020Quote: ... 50 μg/ml cycloheximide) and centrifuged for 3 h at 55,000 g and 4 °C in a Type 70 Ti rotor (Beckman). The pellet was dissolved in 1% SDS ...
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bioRxiv - Microbiology 2020Quote: ... the 100,000xg centrifugation was performed for 1h at 4°C using a SW41 TI swinging bucket rotor (Beckman Coulter). The pellet was then resuspended in 50 μL or 1500 μL of D-MEM for subsequent analysis.
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bioRxiv - Microbiology 2021Quote: ... Filtered supernatants were pelleted at 60,000 g for 16 hours at 4 °C in a SW28 Ti rotor (Beckman), and the resulting pellet was resuspended in TNE buffer (50 mM Tris-HCl [pH 7.4] ...
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bioRxiv - Molecular Biology 2020Quote: ... The reactions were then spun for 20 min at 120,000 rpm at 4°C (TLA120.1 rotor, Beckman Coulter, USA) to remove aggregates and ribosomes ...
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bioRxiv - Microbiology 2020Quote: ... Supernatant was transferred to a fresh tube and further clarified by centrifugation at 18,000 x g for 10 min at 4°C in a microfuge (Beckman), and supernatant transferred to a fresh tube.
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bioRxiv - Microbiology 2020Quote: ... Cell lysate was then clarified 200 x g for 10 min at 4°C in an Allegra centrifuge (Beckman). Supernatant was transferred to a fresh tube and further clarified by centrifugation at 18,000 x g for 10 min at 4°C in a microfuge (Beckman) ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was clarified by centrifugation (183,960 x g, 88 min, 4°C) in a Type 70Ti rotor (Beckman). The supernatant was mixed with 2 ml of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences ...
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bioRxiv - Molecular Biology 2022Quote: ... Gradients were spun for 3 hours at 4° C and 36,000 RPM (221,632 x g) on SW41-Ti rotor (Beckman Coulter). Monosome fractions were isolated from each sucrose gradient with Brandel Density Gradient Fractionator using 1.5 mL/min flow speed and 60% CsCl ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was clarified via centrifugation (66,000 x g, 30 min, 4°C) in a Type 70 Ti rotor (Beckman). The supernatant was mixed with 1.5 ml of anti-FLAG M2 affinity gel (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... sonicated using a sonicator (Smurt, Microtec) and ultracentrifuged at 100,000 x g for 60 minutes at 4°C (Optima XE-90, Beckman). Protein concentration of the bacterial membrane suspension was determined with Bio-Rad protein assay (BioRad).
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bioRxiv - Cell Biology 2022Quote: ... and centrifuged at 10,000g and 4°C for 30 minutes (SW41 rotor, Optima XPN-100 ultracentrifuge; Beckman Coulter, Inc.) to remove any potential large debris ...
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bioRxiv - Biochemistry 2022Quote: ... 5 mM MgCl 2 and 2 mM DTT and centrifuged at 70,000 x g for 20.5 h at 4 °C in an SW28 rotor (Beckman Coulter). Fractions corresponding to the 40S peak were collected ...
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bioRxiv - Biochemistry 2022Quote: ... Gradients were centrifuged at 42,800 x g for 15 h at 4 °C in an SW28 rotor (Beckman Coulter). Fractions of 1 mL were collected using an AKTA system (Cytiva) ...
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bioRxiv - Microbiology 2022Quote: ... at 4°C for 1.5-2h followed by 180,000 x g (Ti 70i rotor; Beckman Instruments, Inc., Fullerton, CA) at 4°C for 1.5-2h and resuspended in 1mL of phosphate buffered saline (PBS ...
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bioRxiv - Genetics 2022Quote: ... The lysate was centrifuged at 2000 rpm for 10 min at 4 °C in a JA-20 rotor (Beckman). The supernatant from the previous step was centrifuged in a Beckman Coulter high speed centrifuge equipped with a JA-20 rotor at 6000 rpm for 20 min at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... The gradient was centrifuged at 200,000 × g for 20 h at 4°C (35,000 rpm with # Optima-XE SW41 Beckman Coulter). The gradient was collected in 2-mL fractions ...
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bioRxiv - Neuroscience 2020Quote: ... this second supernatant was centrifuged for 4 hrs at 100,000g (Optima MAX-XP ultracentrifuge, TLA-100.3 rotor, Beckman Coulter). Following ultracentrifugation ...
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bioRxiv - Plant Biology 2020Quote: ... and centrifuged at 131,500 × g for 30 min at 4 °C using a SW 32 Ti rotor (Beckman Coulter). Thylakoid membranes sedimented in 1.3 M sucrose cushion were collected and washed with 25 mM Tricine-KOH (pH 7.8) ...
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bioRxiv - Biochemistry 2022Quote: ... mixed for 10 minutes at 4°C and centrifuged at 25,000 rpm for 45 minutes (Beckman Ti-45 rotor). The supernatant was warmed to 37°C for few minutes until it turned cloudy following which it was centrifuged at 11,000 rpm at room temperature for 10 minutes (Beckman JA-20 rotor ...
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bioRxiv - Neuroscience 2022Quote: ... and centrifuged at 24,000 rpm for 2 h at 4°C using a SW-Ti32 swinging bucket (Beckman Coulter). The upper layer was discarded ...
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bioRxiv - Systems Biology 2020Quote: ... the 500xg supernatant from enzymatic digestion of cardiovascular tissues underwent further ultracentrifugation at 10,000xg for 10 minutes at 4°C (Beckman Coulter ...
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bioRxiv - Cell Biology 2020Quote: ... The soluble fractions released by MNase were isolated by taking supernatants after centrifugation at 13,894 rpm (16,000 rcf) at 4 °C for 30 min using SX241.5 rotor in Allegron X-30R (Beckman Coulter). The supernatants were collected and layered onto the 10-22 % linear sucrose gradient solution with buffer SG (15 mM HEPES-KOH [pH 7.4] ...
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bioRxiv - Biochemistry 2021Quote: ... Eluted fractions were pooled and subjected to a 2.5 h ultracentrifugation at 135,500 x g at 4 °C using a TLA-55 rotor (Beckman-Coulter). Finally ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates were loaded on 15-50% sucrose gradient and centrifuged at 35,000 rpm for 2.5 h at 4°C in a SW41 rotor (Beckman Coulter). Polysomes were separated through a live optical density (OD ...
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bioRxiv - Molecular Biology 2020Quote: ... Ten OD260 units were loaded on a 10–50% sucrose gradient and centrifuged at 187,813 × g for 2.75 h at 4 °C in a SW41Ti rotor (Beckman Coulter). The fractions of the gradient were collected ...
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bioRxiv - Cell Biology 2021Quote: ... The crude extract was clarified by high-speed centrifugation (60 min at 36,000 ×g at 4 °C) by Beckman Coulter centrifuge.
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bioRxiv - Plant Biology 2021Quote: ... A soluble fraction was obtained by ultracentrifugation at 200,000 g for 20 minutes at 4°C on a Beckman Optima Ultracentrifuge (Beckman). Two biological replicates for SEC and IEX column separations were prepared in this manner.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... IL-4 and IFN-γ concentrations were determined using DTX 880 Multimode Detector (Beckman Coulter, Inc., Brea, CA, USA) set to 450 nm.
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bioRxiv - Biochemistry 2021Quote: ... The gradient was centrifuged at 100,000 g for 16 hours at 4°C (rotor MLS-50, Beckman Optima MAX). Twelve fractions of 400 μL were collected from the top of the gradient and pelleted by UC at 100,000 g for 2 hours at 4°C (Figure 1) ...
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bioRxiv - Biochemistry 2021Quote: ... and 10 mM NH4Cl) and centrifuged for 16 h at 4°C with 18,000 rpm in an SW60Ti rotor (Beckman). Gradients were hand-fractionated and ribosome concentrations were determined by absorbance at 260 nm (A260 ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were centrifuged at 50,000 rpm (∼166,000 × g) for 3h at 4°C using TLS-55 rotor (Beckman Coulter) in an Optima benchtop ultracentrifuge (Beckman Coulter ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were collected by ultracentrifugation at 220 000 x g for 90 min at 4°C (45Ti rotor, Beckman).
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bioRxiv - Biochemistry 2021Quote: ... and the lysate was clarified by centrifugation at 30,000 x g for 60 min at 4 °C in a JA25.50 rotor in an Avanti J-E centrifuge (Beckman). The clarified lysate was then added to 10 mL of Ni-NTA Agarose beads (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... Pellets were resuspended in 0.9% NaCl and spun at 110,000 × g for 2 hours at 4°C in an XPN-80 ultracentrifuge equipped with a Type 50.4 Ti rotor (Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... The VLPs were concentrated by ultracentrifugation for 2 h at 31,000 rpm at 4 °C (Beckman SW-31Ti rotor), and then resuspended in 500 μL of Ex-Cell 405 medium ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifugation for 30 min at 35,000 rpm at 4°C in a Type 45 Ti rotor (Beckman). Cleared lysate was incubated with 2 mL glutathione-sepharose (Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifugation for 30 min at 35,000 rpm at 4°C in a Type 45 Ti rotor (Beckman). Cleared lysate was incubated with 2 mL Ni-NTA agarose (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were ultracentrifuged at 100,000 g for 30 minutes at 4°C in a Optima XPN-100 ultracentrifuge (Beckman). Supernatants were kept aside as “Supernatant” fraction ...
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bioRxiv - Microbiology 2023Quote: ... All centrifugation steps were performed at 4 °C in an Avanti J-E high-speed fixed-angle centrifuge (Beckman Coulter Life Sciences ...
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bioRxiv - Neuroscience 2023Quote: ... DTT-treated EVs were pelleted at 100,000 x g for 70 min at 4°C in a TLA100 rotor (Beckman). The supernatant ...
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bioRxiv - Neuroscience 2023Quote: ... The prepared gradients were centrifuged at 200,000 x g for 16 h at 4°C in an SW41Ti rotor (Beckman), with no braking during deceleration ...
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bioRxiv - Neuroscience 2023Quote: ... The pellet was resuspended in 4.5 volumes of ice-cold extraction buffer and re- centrifuged at 11,300 RPM for 10 min at 4°C using a Ti45 rotor (Beckman). The supernatant was filtered through a 70 μm pore size cell-strainer and combined with the first supernatant ...
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bioRxiv - Neuroscience 2023Quote: ... The tube was topped up to 23 mL with PBS and centrifuged at 59,300 RPM for 30 min at 4°C using a Ti70 rotor (Beckman). The floating lipid layer and supernatant were discarded and the pellet was transferred to a 2 mL tube in 50 μL PBS per g initial grey matter ...
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bioRxiv - Neuroscience 2023Quote: ... The homogenate was centrifuged at 11,300 revolutions per minute (RPM) for 10 min at 4°C using a Ti45 rotor (Beckman). The supernatant was filtered through a 70 μm pore size cell-strainer and retained on ice ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was transferred to fresh tubes and centrifuged at 100,000 x g for 70 min at 4°C using a Ti45 rotor (Beckman). The 100,000 x g pellets were resuspended in 1 mL ice-cold PBS and brought to 60 mL using PBS ...
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bioRxiv - Microbiology 2022Quote: ... Cell debris was removed by centrifugation at 14,800 rpm for 20 minutes 4 °C in F241.5P rotor using 149 Microfuge 22R centrifuge (Beckman Coulter). The supernatant was combined with 100 µL of ANTI-FLAG M2 Affinity Gel (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... The lysate was centrifuged at 40,000 rpm for 50 min at 4°C (45 Ti fixed-angle rotor, Beckman) and the transporter-Fab complex was isolated by affinity chromatography using Strep-Tactin resin ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed by extrusion using a Teflon Dounce homogenizer and clarified by centrifugation at 30000g for 40 minutes at 4°C using a Ja 25.50 rotor (Beckman). The clarified lysate was incubated with 2 ml of pre-equilibrated Protein C affinity resin (Roche ...