Labshake search
Citations for Stemcell Technologies :
401 - 450 of 4606 citations for Rat T Cell Surface Glycoprotein CD8 Beta Chain CD8B ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: from CD4-T cells following the protocol described in the EasySep PE-selection kit (STEMCELL technologies). Cells from the negative fraction (CD4+CD25- ...
-
bioRxiv - Immunology 2019Quote: Human T-cell subsets were isolated from leukapheresis produts using negative selection kits from STEMCELL Technologies. Rosette Sep (Stemcell Technologies ...
-
bioRxiv - Immunology 2021Quote: ... negative selection was performed using EasySep Human T cell isolation kit (#17951, Stemcell technologies, Vancouver, Canada). The CD3+ T cells were labelled with 1µM Cell Trace Violet dye (#C34557 ...
-
bioRxiv - Bioengineering 2023Quote: ... ImmunoCult-XF T Cell Expansion Medium and immunomagnetic negative selection kits were purchased from STEMCELL Technologies. FITC-dextran molecules (500 kDa ...
-
bioRxiv - Bioengineering 2024Quote: ... Tregs were isolated using the EasySep human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (StemCell Tech.) and cultured in X-Vivo15 ...
-
bioRxiv - Immunology 2021Quote: ... ICM T cells were stimulated with ImmunoCult™ Human CD3/CD28 T Cell Activators (25 μl/ml, STEMCELL Technologies). TAC T cells were stimulated with T Cell CD3/CD28 TransAct™ ...
-
bioRxiv - Molecular Biology 2021Quote: ... Total CD4+ T cells were separated by negative selection using EasySep Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies). Primary CD4+ T cells were plated at a concentration of 1,5x10 6 cells/mL left overnight for recovery ...
-
bioRxiv - Immunology 2020Quote: ... we treated CD4+ T cells with either αCD3αCD28 (ImmunoCult™ Human CD3/CD28 T Cell Activator; STEMCELL Technologies, 10971) or IFN I (1:1000 ...
-
bioRxiv - Molecular Biology 2023Quote: ... T cells were cultured at ∼1-2 x 106mL in ImmunoCult-XF T cell expansion medium (StemCell Technologies, Canada) supplemented with Penicillin-Streptomycin (Gibco ...
-
bioRxiv - Cancer Biology 2023Quote: ... 5 x 105 CD3+ T cells were plated in 0.5 ml T cell expansion medium (Stemcell Technologies, Cologne, Germany) with 50 IE/ml IL-2 ...
-
bioRxiv - Bioengineering 2024Quote: ... CD4+ T cells were activated on-chip by adding ImmunoCult™ Human CD3/CD28 T Cell Activator (StemCell Technologies) to the culture media ...
-
bioRxiv - Immunology 2024Quote: ... CD3 T cells were isolated by negative selection following manufacturer’s protocol (RosetteSep Human T cell enrichment cocktail, STEMCELL Technologies). Following isolation ...
-
bioRxiv - Immunology 2023Quote: ... T cells were obtained from whole blood using the RosetteSep™ Human T Cell Enrichment Cocktail (StemCell Technologies, 15061), followed by density gradient separation using Ficoll® Paque Plus (Cytiva Life Sciences ...
-
bioRxiv - Immunology 2020Quote: ... Purity and viability of isolated T cells was assessed using flow cytometry prior to cryopreservation of isolated T cells in CryoStore CS10 cryopreservation media (Stem Cell Technologies) at a density of 1-1.5×107 cells per ml.
-
bioRxiv - Synthetic Biology 2023Quote: ... T cells were then resuspended at 1×106 cells/mL in ImmunoCult™-XF T Cell Expansion Medium (Stemcell Technologies, Cat.#10981) supplemented with Human Recombinant IL-2 (12.5 ng/mL ...
-
bioRxiv - Cancer Biology 2023Quote: ... After an additional 48 hours non-adhered cells were discarded and adhered T-ALL cells were separated from glioblastoma cells using EasySep Human CD3 positive Selection kit II (Stem Cell Technologies) and viability was determined by trypan blue staining (Bio-Rad) ...
-
bioRxiv - Immunology 2023Quote: ... T cells from the transgenic mouse splenocytes were enriched by magnetic isolation using the EasySepTM Mouse T Cell Isolation Kit (Stem Cell Technologies). T cells were centrifuged and resuspended in PBS ...
-
bioRxiv - Immunology 2021Quote: 0.25-0.5 million non-parenchymal cells were used with the EasySepTM Mouse T cell Isolation Kit (Stemcell Technologies, Cat.# 19851) for negative selection of T cells ...
-
bioRxiv - Biochemistry 2020Quote: CD8+ or total T cells were isolated from spleens with an EasySep(tm) T Cell Isolation Kit according to the manufacturer’s instructions (STEMCELL Technologies).
-
bioRxiv - Immunology 2022Quote: ... Naive human CD4+ T cells were isolated using the EasySep Human Naïve CD4 TCell Iso Kit II (Stem Cell Technologies) according to the manufacturers’ instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... followed by sorting of cells using the SONY SH800 sorter or obtained with the naïve human CD4+ T cell isolation kit (StemCell Technology). For T helper cell culture ...
-
bioRxiv - Cell Biology 2024Quote: ... MDM cells were detached from the growth surface by accutase (StemCell Technologies) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2021Quote: ... Primary human T cells were isolated using negative selection per the manufacturer’s instructions (RosetteSep Human T Cell Enrichment Cocktail, STEMCELL Technologies). T cells were counted using a Countess II FL Automated Cell Counter (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2021Quote: ... Total CD4+ T cells were separated by negative selection using RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies). Primary CD4+ T cells were plated at a concentration of 1,5×10 6 cells/mL left overnight for recovery and infected ...
-
bioRxiv - Immunology 2020Quote: CD4+ T cells were isolated from healthy adults using the RosetteSep human CD4+ T cell enrichment cocktail (STEMCELL Technologies, 15062) followed by a density gradient isolation (STEMCELL Technologies ...
-
bioRxiv - Immunology 2020Quote: ... PBMCs were used to isolate T cells by CD3-negative selection (EasySep Human T cells Isolation, STEMCELL Technologies, Vancouver, Canada). Tumor-infiltrating lymphocytes (TILs ...
-
bioRxiv - Cancer Biology 2023Quote: ... total PBMCs or purified human naïve CD4+ T cells were stimulated with the ImmunoCult human T cell activator (StemCell Technologies) in the presence of either DMSO or soquelitinib for 72 hours ...
-
Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from elutriated lymphocytes by negative selection using RosetteSep CD4+ T cell enrichment cocktail (StemCell Technologies) followed by Ficoll/Hypaque (Amersham Biosciences ...
-
bioRxiv - Bioengineering 2023Quote: Primary human CD3+ T cells from 3 unique donors were isolated by negative selection (T Cell Enrichment Cocktail, Stemcell Technologies) from peripheral blood and were grown in RPMI + 10% FBS + 1% PS supplemented with 50 U/mL of IL2 ...
-
bioRxiv - Immunology 2024Quote: ... CD4 T cells were isolated from the spleens of young (2- to 3-month-old) mice spleen using a magnetic microbeads negative selection kit (EasySep Mouse CD4 T Cell Isolation Kit; STEMCELL Technologies) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2021Quote: ... T cells were cryopreserved in CryoStor10 (STEMCELL technologies). After thawing ...
-
bioRxiv - Biochemistry 2023Quote: Human peripheral blood Pan-T cells (Stemcell Technologies) were thawed and expanded following manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... and passaged using T ReLeSR (Stem Cell Technologies). Quality control was performed with karyotyping (G-banding ...
-
bioRxiv - Immunology 2022Quote: Preparation of cells: Human CD4+ cells were isolated from buffy coats using EasySep™ Human CD4+ T Cell Isolation Kit (Stemcell Tech, # 17952), resuspended with complete RPMI (cRPMI ...
-
bioRxiv - Immunology 2021Quote: Purified CD4+CD25− T cells were magnetically isolated from spleen and peripheral lymph node mononuclear cells using an EasySep magnetic T cell negative isolation kit (Stem Cell Technologies, Inc.) with addition of a biotin anti-mouse CD25 antibody (0.5 μg/mL ...
-
bioRxiv - Immunology 2020Quote: ... CD4+ T-cells were isolated from the pooled cell suspensions using an EasySep(tm) Mouse CD4+ T Cell Isolation Kit from Stemcell Technologies (Vancouver, Canada) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... were purified via negative selection (EasySep™ Mouse CD4+ T cell Isolation Kit, STEMCELL Technologies, Lot: 18H94653). Purified CD4+ T cells were cultured for 72 hours with interleukin-12 (IL-12 ...
-
bioRxiv - Immunology 2020Quote: ... For Il2ra time course naïve CD4+ T cells were enriched from splenocytes using EasySep kit (StemCell Technologies). Approximately 100,000 cells were activated using 2ug/ml plate bound anti-CD3/CD28 ...
-
bioRxiv - Immunology 2023Quote: ... Lymph node CD8α+ T cells were isolated and sorted using the CD8α Positive Selection Kit (Stemcell Technologies). Isolated cells were washed and resuspended at 1 x 106 cells/ml in stimulation media ...
-
bioRxiv - Immunology 2023Quote: ... and monocytes were isolated from PBMCs by EasySep Human T Cell Isolation Kit (StemCell Technologies, Vancouver, Canada), MACSxpress Whole Blood NK Cell Isolation Kit (Miltenyi Biotec ...
-
bioRxiv - Immunology 2019Quote: ... ImmunocultTM-XF T cell expansion medium (STEM CELL Technologies; Vancouver, Canada). Control comparisons were performed using RPMI 1640 medium with L-glutamine and phenol red (Thermo Fisher ...
-
bioRxiv - Immunology 2023Quote: ... Cells were maintained in ImmunoCult T Cell Expansion Medium (STEMCELL Technologies) supplemented with 20 ng/mL of recombinant mouse interleukin (IL)- 2 (R&D systems) ...
-
bioRxiv - Bioengineering 2023Quote: Human peripheral blood CD4+ T cells (Stem Cell Technologies, 200-0165) were cultured in ImmunoCult-XF T Cell Expansion media (Stem Cell Technologies ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Regulatory T cells were isolated using the EasySep human CD4+CD127lowCD25+ regulatory T cell isolation kit according to manufacturer’s protocol (STEMCELL Technologies, 18063). Primary T cells were cultured in X-Vivo 15 media (Lonza ...
-
bioRxiv - Cancer Biology 2021Quote: Aliquots of fresh blood or frozen ficoll-separated peripheral blood cells were fractionated using EasySep Human Myeloid Positive Selection and EasySep Human T Cell Enrichment kits (StemCell Technologies. DNA was extracted from the fractionated subsets using MagMAX DNA Multi-Sample Ultra 2.0 Kit (ThermoFisher ...
-
bioRxiv - Immunology 2021Quote: Memory and effector CD4 T cells were purified from total splenocytes isolated from immunized mice using EasySepTM Mouse Memory CD4+ T Cell Isolation Kit (StemCell Technologies) as per manufacturer instructions ...
-
bioRxiv - Immunology 2022Quote: ... and B and T cells were isolated using EasySep Negative selection Mouse B and T cell Enrichment Kits according to the manufacturers protocol (StemCell Technologies). Cells were then suspended in transfer buffer (10 mM HEPES ...
-
bioRxiv - Bioengineering 2023Quote: ... Human T cells were purified (> 96% purity) by magnetic negative selection using an EasySep Human T Cell Isolation Kit (Cat#17951, Stemcell Technologies) according to the manufacturer’s instruction ...
-
bioRxiv - Molecular Biology 2023Quote: CD4+ cells were isolated from freshly dissociated mouse spleen using the EasySep Mouse CD4+ T cell Isolation Kit (STEMCELL Technologies, 19852). CD4+ cells were then immediately used for bulk ATAC-seq ...
-
bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were isolated from thawed PBMCs by negative magnetic selection using the EasySep Human CD4 T Cell Enrichment Kit (STEMCELL Technologies) according to the manufacturer’s protocol.