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Citations for Stemcell Technologies :
451 - 500 of 4606 citations for Rat T Cell Surface Glycoprotein CD8 Beta Chain CD8B ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: The CD4+ T-cell subpopulation was isolated from the CD14- fraction of PBMCs using the Easysep human CD4+ T-cell isolation kit (Stemcell Technology) as per the instructions of the manufacturer ...
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bioRxiv - Immunology 2024Quote: ... CD4+ cells were isolated from pooled splenic suspensions by magnetic negative selection using EasySep Mouse CD4+ T Cell Isolation Kit (StemCell Technologies) according to manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... Single cell suspensions were generated and enriched for CD4+ T cells by negative selection using EasySep magnetic bead kit (STEMCELL Technologies) and stained with anti-mouse CD4 (RM4-5 ...
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bioRxiv - Microbiology 2020Quote: ... CD4+ T cells were enriched from blood of healthy donors using RosetteSep Human CD4+ T cell enrichment cocktail (Stemcell Technologies, Canada) according to the manufacturer’s instructions followed by Ficoll density gradient centrifugation ...
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bioRxiv - Immunology 2023Quote: ... The percentage of CD3+ cells was determined by flow cytometry and T cells were stimulated with Immunocult Human CD3/CD28/CD2 T cell activator (StemCell Technologies) per fabricator instructions in IMDM (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... All MSCs were cultured on ACF Cell Attachment Substrate coated surfaces (Stem Cell Technologies; Catalogue #07130). ACF Cell Dissociation Kit (Stem Cell Technologies ...
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bioRxiv - Neuroscience 2021Quote: T lymphocytes were isolated from the spleen of C57BL/6J mice using a negative selection isolation kit (EasySep™ Mouse T Cell Isolation Kit, StemCell Technologies). Cells were plated in a 48 wells plate (Corning ...
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bioRxiv - Genomics 2022Quote: CD4 T-cells were isolated from 10-20 ml of peripheral blood using EasySep Direct Human CD4+ T cell Isolation kit (Stem Cell Technologies, cat #19662). The isolated CD4 T-cells were stored in Crystor CS10 (Stem Cell Technologies ...
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bioRxiv - Immunology 2022Quote: ... and/or rat serum (2%; Stem Cell Technologies) for 5min on ice prior to adding fluorescent antibodies (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... and CD4 T cells were isolated by immunomagnetic positive selection (EasySep Human CD4 positive selection kit; Stemcell Technologies). Sex and age for these individuals can be found in the metadata corresponding to the RNA-Seq data available from the Sequence Read Archive under accession number PRJNA832136.
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bioRxiv - Molecular Biology 2023Quote: ... starting from freshly isolated PBMCs from 69 PLWH using the EasySep CD4 T cell isolation kit (Stemcell Technologies). CD4+ T cells were frozen as dry pellet at −80°C prior to DNA extraction with the DNeasy Blood & Tissue kit (Qiagen) ...
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bioRxiv - Immunology 2023Quote: ... For Il-2ra time course naïve CD4+ T cells were enriched from splenocytes using EasySep kit (StemCell Technologies). Approximately 100,000 cells were activated using 2ug/ml plate bound anti-CD3/CD28 ...
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bioRxiv - Cell Biology 2023Quote: Primary human CD4+ T cells were isolated from leukocyte cones using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies). T cells were cultured at 37°C and 5% CO2 in RPMI 1640 (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: Human T cells were isolated from healthy donors who provided informed consent using RosetteSep Human T-Cell Enrichment Cocktail (STEMCELL Technologies, Canada) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Primary CD4+ T cells were isolated from peripheral blood mononuclear cells (PBMCs) and enriched via negative selection with an EasySep Human CD4+ T Cell Isolation Kit (Stemcell Technologies, Canada). Primary CD4+ T cells were cultured at 37°C in RPMI supplemented with 10% FBS ...
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bioRxiv - Immunology 2021Quote: 1 × 105 memory CD4+ T cells freshly purified from PBMC by negative selection and magnetic separetion(EasySep™ Human memory CD4+ T cell enrichment kit, STEMCELL Technologies) were cocultured at 1:1 ratio for 6 days with anti-CD3/CD28–coated beads (Dynabeads ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from pooled splenic cell suspensions by magnetic negative selection using EasySep™ Mouse CD4+ T Cell Isolation Kit (StemCell Technologies) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... in ImmunoCult-XF T Cell Expansion Medium (STEMCELL Technologies) with 5% fetal bovine serum (ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... CD3+ T cells were isolated by RosetteSep (STEMCELL Technologies).
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bioRxiv - Immunology 2021Quote: ... naïve CD4+ T cells were enriched (StemCell Technologies; 19555) from healthy donor human PBMCs and cultured in 96-well plates ...
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bioRxiv - Microbiology 2022Quote: ... CD4+ T cells were negatively enriched (STEMCELL Technologies, #19052), pelleted ...
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bioRxiv - Cancer Biology 2023Quote: ... and CD28 (ImmunoCult human T cell activator, StemCell Technologies) in total volume of 200 μl complete RPMI medium in a 96-well U bottom plate in the presence of either DMSO or soquelitinib for 48 hours ...
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bioRxiv - Molecular Biology 2024Quote: Primary T-cells were purchased from StemCell Technologies (#70024). Cells were cultured in X-Vivo 15 media supplemented with 5% human serum and 10 ng/mL of IL-2 ...
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bioRxiv - Biochemistry 2022Quote: ... Total T-cells were isolated by negative selection (Stem Cell Technologies #17951) and activated with CD3/CD28 Dynabeads (Life Technologies 11132D ...
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bioRxiv - Bioengineering 2023Quote: ... and ImmunoCult Human CD3/CD28 T Cell Activator (Stem Cell Technologies, 10971) was added at 5 μL/mL to a total volume to 200 μL ...
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bioRxiv - Microbiology 2019Quote: ... CD4+ T cells at 106 cells/mL were stimulated with 25 μL/mL ImmunoCult™ Human CD3/CD28 T Cell Activator (STEMCELL Technologies, Vancouver, Canada, catalogue #10991). At day 3 post-stimulation ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs were immediately processed to isolate CD4+ T cells by negative selection using the EasySep Human CD4+ T Cell Isolation Cocktail (StemCell Technologies, Cat. #17952) according to manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... the CAR T cells were recovered by two days of cultivation in ImmunoCult™-XF T Cell Expansion Medium (Stemcell Technologies, Cat.#10981) supplemented with Human Recombinant IL-2 (12.5 ng/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with NeuroCult proliferation kit (mouse & rat, STEMCELL Technologies, #05702), human recombinant EGF (20 ng/mL ...
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bioRxiv - Cell Biology 2021Quote: ... T cells were purified from peripheral blood samples by the negative selection EasySepTM Human T cell Enrichment Kit (Catalog #17951, StemCell Technologies, Vancouver, Canada). Enriched T cells were stained with Cell Proliferation Dye eFluorTM 450 (Catalog #65-0842 ...
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bioRxiv - Bioengineering 2020Quote: ... PBMCs were further purified for CD3+ cells by immunomagnetic negative selection using the EasySep Human T-cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). T cells were frozen at 10–20 × 106 cells per 1 mL of Cryostor CS10 (STEMCELL Technologies ...
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bioRxiv - Immunology 2023Quote: ... Tcon and Treg cells were isolated from the spleen by pre-enrichment with EasySep Mouse CD4+ T cell Isolation Kit (STEMCELL Technologies, Cat# 19852), and FACS sorting ...
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bioRxiv - Immunology 2023Quote: ... naïve CD4+ T-cells were isolated via two-stage negative selection with EasySep™ Human Naïve CD4+ T-Cell Isolation Kit II (Stemcell Technologies Cat# 17555); and CD8+ T-cells were isolated by negative selection from PBMCs with Stem Cell Technology Easy Sep CD8+ kit ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were isolated from fresh or frozen PBMCs by negative selection using the EasySep™ human CD4+ T cell isolation kit according to the manufacturer’s instructions (Stem Cell Technology, USA), and activated with CD2/CD3/CD28 magnetic beads (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2024Quote: Human CD4+CCR6+CXCR3- T cells were isolated from human donor leukopaks using EasySep™ Human Th17 Cell Enrichment Kit (StemCell Technologies, 18162). To obtain large quantities of cells ...
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bioRxiv - Genomics 2023Quote: ... The washed PBMCs were resuspended at 200E6 cells/mL in EasySep buffer and isolated with the EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies, #18063), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells were isolated from single-cell suspensions from spleen tissue by negative selection using the EasySep Mouse CD4+ T cell Isolation Kit (# 19852, StemCell Technologies, Cambridge, MA) and differentiated into different T helper (Th ...
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bioRxiv - Immunology 2020Quote: ... and used directly for total T cell enrichment by negative magnetic selection using Easysep magnetic separation kits (STEMCELL Technologies). Cell suspension was stained with anti-CD4 (RPA-T4) ...
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bioRxiv - Cell Biology 2023Quote: ... CD4+ T cells were isolated from monocyte-depleted PBMCs using EasySepTM Human CD4+ Selection Kit II (StemCell Technologies Ltd.) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... APCs from NOD spleens were obtained by depleting T cells using the Mouse CD90.2 Positive Selection Kit (STEMCELL Technologies) then pulsed with 15 µM of the indicated peptides and co-cultured with Tregs/Tconvs at a 1:1 ratio ...
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bioRxiv - Neuroscience 2023Quote: ... and Naïve CD4+ T cell were isolated from splenocytes with commercial kits (STEMCELL Technologies, Cat No. 19852, 19853, 19765) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... and NKX2.5EGFP/+ hPSCs31 were maintained on Matrigel (Corning)-coated surfaces in mTeSR1 (STEMCELL Technologies) as previously described44 ...
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bioRxiv - Bioengineering 2020Quote: ... were maintained on Matrigel (Corning)-coated surfaces in mTeSR1 (STEMCELL Technologies) as previously decribed46 ...
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bioRxiv - Cell Biology 2022Quote: ... cryopreserved CD4+ human cord blood T cells were purchased from Stem Cell Technologies. T cells were isolated from mixed T and B cell cultures using nylon wool separation96 ...
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bioRxiv - Genomics 2021Quote: ... 2×107 T cells were aliquoted into CryoStor cryopreservation media (Stem Cell technologies) for long-term storage.
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bioRxiv - Immunology 2023Quote: ... Isolated T cells were cryopreserved in CryoStor CS10 cryopreservation medium (Stem Cell Technologies).
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bioRxiv - Bioengineering 2023Quote: ... were cultured in ImmunoCult XF T Cell Expansion media (Stem Cell Technologies, 10981) at 37 °C with 5% CO2 ...
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bioRxiv - Bioengineering 2023Quote: ... were cultured in ImmunoCult-XF T Cell Expansion media (Stem Cell Technologies, 10981) at 37 °C with 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... (2) ImmunoCult™-XF T Cell Expansion Medium (STEMCELL Technologies) was supplemented with 1X Penicillin Streptomycin solution (ICM) ...
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bioRxiv - Microbiology 2020Quote: ... Immunocult human CD3/CD28 T cell activator (STEMCELL Technologies, USA) was used to stimulate the PBMCs as a positive control for immunological activation.